Clostridium botulinum C str. Eklund

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium botulinum; Clostridium botulinum C

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2552 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1B7Y6|B1B7Y6_CLOBO Uncharacterized protein OS=Clostridium botulinum C str. Eklund OX=445337 GN=CBC_A1498 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.49ADD4 pKa = 3.87VDD6 pKa = 3.6NDD8 pKa = 3.78LCIGCGLCPSICPEE22 pKa = 4.05VFDD25 pKa = 5.14MGDD28 pKa = 3.16DD29 pKa = 3.81GKK31 pKa = 11.28AHH33 pKa = 4.95VTVDD37 pKa = 3.61EE38 pKa = 4.37VEE40 pKa = 4.33EE41 pKa = 4.1ASKK44 pKa = 11.23DD45 pKa = 3.69SASEE49 pKa = 3.9ARR51 pKa = 11.84QSCPVEE57 pKa = 4.53AITVEE62 pKa = 4.32EE63 pKa = 4.2

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1BDL2|B1BDL2_CLOBO HTH-type transcriptional regulator SinR OS=Clostridium botulinum C str. Eklund OX=445337 GN=CBC_A0984 PE=4 SV=1
MM1 pKa = 7.33ARR3 pKa = 11.84KK4 pKa = 9.97AMIEE8 pKa = 3.98KK9 pKa = 9.41WNKK12 pKa = 6.18TPKK15 pKa = 9.88YY16 pKa = 8.01STRR19 pKa = 11.84AYY21 pKa = 8.1TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 7.08SVLKK35 pKa = 10.64KK36 pKa = 10.27YY37 pKa = 9.54GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.7LAYY49 pKa = 10.44RR50 pKa = 11.84GQIPGCRR57 pKa = 11.84KK58 pKa = 10.14ASWW61 pKa = 3.01

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2552

0

2552

754814

31

2861

295.8

33.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.24 ± 0.052

1.241 ± 0.017

5.531 ± 0.046

7.284 ± 0.073

4.25 ± 0.037

6.179 ± 0.05

1.346 ± 0.017

10.189 ± 0.066

9.967 ± 0.07

8.711 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.026

6.819 ± 0.053

2.714 ± 0.03

2.364 ± 0.026

3.273 ± 0.031

6.213 ± 0.043

4.853 ± 0.039

6.472 ± 0.045

0.637 ± 0.015

4.116 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski