Amino acid dipepetide frequency for Clostridium botulinum C str. Eklund

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.272AlaAla: 3.272 ± 0.102	0.645AlaCys: 0.645 ± 0.03	2.269AlaAsp: 2.269 ± 0.059	3.209AlaGlu: 3.209 ± 0.075	2.312AlaPhe: 2.312 ± 0.05	3.311AlaGly: 3.311 ± 0.083	0.803AlaHis: 0.803 ± 0.034	5.776AlaIle: 5.776 ± 0.104	4.877AlaLys: 4.877 ± 0.09	5.315AlaLeu: 5.315 ± 0.104	1.587AlaMet: 1.587 ± 0.049	2.544AlaAsn: 2.544 ± 0.059	1.361AlaPro: 1.361 ± 0.046	1.317AlaGln: 1.317 ± 0.048	1.902AlaArg: 1.902 ± 0.053	3.182AlaSer: 3.182 ± 0.073	2.708AlaThr: 2.708 ± 0.065	3.666AlaVal: 3.666 ± 0.081	0.306AlaTrp: 0.306 ± 0.021	2.034AlaTyr: 2.034 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.578CysAla: 0.578 ± 0.029	0.221CysCys: 0.221 ± 0.019	0.775CysAsp: 0.775 ± 0.035	0.816CysGlu: 0.816 ± 0.037	0.493CysPhe: 0.493 ± 0.028	1.039CysGly: 1.039 ± 0.042	0.237CysHis: 0.237 ± 0.019	1.233CysIle: 1.233 ± 0.041	1.13CysLys: 1.13 ± 0.043	0.88CysLeu: 0.88 ± 0.042	0.302CysMet: 0.302 ± 0.021	0.938CysAsn: 0.938 ± 0.038	0.468CysPro: 0.468 ± 0.029	0.184CysGln: 0.184 ± 0.016	0.404CysArg: 0.404 ± 0.024	0.813CysSer: 0.813 ± 0.034	0.611CysThr: 0.611 ± 0.026	0.729CysVal: 0.729 ± 0.033	0.058CysTrp: 0.058 ± 0.009	0.499CysTyr: 0.499 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	2.571AspAla: 2.571 ± 0.056	0.594AspCys: 0.594 ± 0.027	2.897AspAsp: 2.897 ± 0.072	4.567AspGlu: 4.567 ± 0.089	2.491AspPhe: 2.491 ± 0.057	3.239AspGly: 3.239 ± 0.072	0.543AspHis: 0.543 ± 0.026	6.749AspIle: 6.749 ± 0.097	5.886AspLys: 5.886 ± 0.096	4.508AspLeu: 4.508 ± 0.071	1.546AspMet: 1.546 ± 0.044	3.627AspAsn: 3.627 ± 0.078	1.204AspPro: 1.204 ± 0.045	0.696AspGln: 0.696 ± 0.034	1.758AspArg: 1.758 ± 0.05	3.194AspSer: 3.194 ± 0.063	2.699AspThr: 2.699 ± 0.058	3.696AspVal: 3.696 ± 0.073	0.348AspTrp: 0.348 ± 0.026	2.497AspTyr: 2.497 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	3.806GluAla: 3.806 ± 0.086	0.8GluCys: 0.8 ± 0.041	4.4GluAsp: 4.4 ± 0.085	6.706GluGlu: 6.706 ± 0.121	3.056GluPhe: 3.056 ± 0.075	3.808GluGly: 3.808 ± 0.071	0.909GluHis: 0.909 ± 0.034	7.065GluIle: 7.065 ± 0.103	7.953GluLys: 7.953 ± 0.125	6.508GluLeu: 6.508 ± 0.108	1.814GluMet: 1.814 ± 0.051	5.559GluAsn: 5.559 ± 0.09	1.318GluPro: 1.318 ± 0.045	1.892GluGln: 1.892 ± 0.061	2.367GluArg: 2.367 ± 0.064	3.503GluSer: 3.503 ± 0.065	2.768GluThr: 2.768 ± 0.066	5.067GluVal: 5.067 ± 0.083	0.436GluTrp: 0.436 ± 0.023	3.105GluTyr: 3.105 ± 0.073	0.0GluXaa: 0.0 ± 0.0
Phe	2.13PheAla: 2.13 ± 0.061	0.57PheCys: 0.57 ± 0.027	2.375PheAsp: 2.375 ± 0.059	2.45PheGlu: 2.45 ± 0.055	1.722PhePhe: 1.722 ± 0.054	2.65PheGly: 2.65 ± 0.067	0.556PheHis: 0.556 ± 0.032	4.609PheIle: 4.609 ± 0.096	4.39PheLys: 4.39 ± 0.091	3.764PheLeu: 3.764 ± 0.082	1.179PheMet: 1.179 ± 0.04	3.217PheAsn: 3.217 ± 0.061	1.081PhePro: 1.081 ± 0.038	0.974PheGln: 0.974 ± 0.037	1.21PheArg: 1.21 ± 0.035	2.973PheSer: 2.973 ± 0.073	2.21PheThr: 2.21 ± 0.046	2.403PheVal: 2.403 ± 0.065	0.277PheTrp: 0.277 ± 0.019	1.757PheTyr: 1.757 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	3.753GlyAla: 3.753 ± 0.09	0.89GlyCys: 0.89 ± 0.037	3.116GlyAsp: 3.116 ± 0.067	4.18GlyGlu: 4.18 ± 0.077	3.003GlyPhe: 3.003 ± 0.078	3.997GlyGly: 3.997 ± 0.083	1.007GlyHis: 1.007 ± 0.034	6.598GlyIle: 6.598 ± 0.115	5.845GlyLys: 5.845 ± 0.115	5.06GlyLeu: 5.06 ± 0.084	1.734GlyMet: 1.734 ± 0.051	3.386GlyAsn: 3.386 ± 0.071	1.195GlyPro: 1.195 ± 0.044	1.439GlyGln: 1.439 ± 0.048	2.132GlyArg: 2.132 ± 0.059	3.396GlySer: 3.396 ± 0.073	3.393GlyThr: 3.393 ± 0.083	4.503GlyVal: 4.503 ± 0.077	0.421GlyTrp: 0.421 ± 0.025	2.744GlyTyr: 2.744 ± 0.075	0.0GlyXaa: 0.0 ± 0.0
His	0.672HisAla: 0.672 ± 0.029	0.18HisCys: 0.18 ± 0.013	0.733HisAsp: 0.733 ± 0.034	0.804HisGlu: 0.804 ± 0.036	0.572HisPhe: 0.572 ± 0.03	0.931HisGly: 0.931 ± 0.035	0.282HisHis: 0.282 ± 0.021	1.44HisIle: 1.44 ± 0.043	1.215HisLys: 1.215 ± 0.035	1.108HisLeu: 1.108 ± 0.037	0.34HisMet: 0.34 ± 0.025	0.839HisAsn: 0.839 ± 0.031	0.575HisPro: 0.575 ± 0.03	0.338HisGln: 0.338 ± 0.021	0.507HisArg: 0.507 ± 0.024	0.885HisSer: 0.885 ± 0.034	0.674HisThr: 0.674 ± 0.028	0.709HisVal: 0.709 ± 0.035	0.099HisTrp: 0.099 ± 0.01	0.56HisTyr: 0.56 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.482IleAla: 5.482 ± 0.08	1.354IleCys: 1.354 ± 0.045	6.13IleAsp: 6.13 ± 0.096	7.147IleGlu: 7.147 ± 0.1	4.208IlePhe: 4.208 ± 0.095	6.211IleGly: 6.211 ± 0.099	1.297IleHis: 1.297 ± 0.04	9.764IleIle: 9.764 ± 0.174	10.025IleLys: 10.025 ± 0.134	9.212IleLeu: 9.212 ± 0.13	2.401IleMet: 2.401 ± 0.058	7.053IleAsn: 7.053 ± 0.108	3.365IlePro: 3.365 ± 0.064	2.41IleGln: 2.41 ± 0.056	3.047IleArg: 3.047 ± 0.066	6.951IleSer: 6.951 ± 0.109	5.132IleThr: 5.132 ± 0.091	6.523IleVal: 6.523 ± 0.1	0.509IleTrp: 0.509 ± 0.027	3.666IleTyr: 3.666 ± 0.079	0.0IleXaa: 0.0 ± 0.0
Lys	4.799LysAla: 4.799 ± 0.089	1.066LysCys: 1.066 ± 0.047	6.522LysAsp: 6.522 ± 0.099	9.53LysGlu: 9.53 ± 0.132	3.821LysPhe: 3.821 ± 0.078	5.53LysGly: 5.53 ± 0.099	1.229LysHis: 1.229 ± 0.04	9.213LysIle: 9.213 ± 0.139	9.556LysLys: 9.556 ± 0.118	8.319LysLeu: 8.319 ± 0.118	2.557LysMet: 2.557 ± 0.058	7.672LysAsn: 7.672 ± 0.11	2.224LysPro: 2.224 ± 0.069	2.532LysGln: 2.532 ± 0.061	3.324LysArg: 3.324 ± 0.064	5.611LysSer: 5.611 ± 0.097	4.295LysThr: 4.295 ± 0.074	6.569LysVal: 6.569 ± 0.098	0.639LysTrp: 0.639 ± 0.029	4.659LysTyr: 4.659 ± 0.093	0.0LysXaa: 0.0 ± 0.0
Leu	4.588LeuAla: 4.588 ± 0.086	1.023LeuCys: 1.023 ± 0.041	4.979LeuAsp: 4.979 ± 0.088	6.074LeuGlu: 6.074 ± 0.095	3.278LeuPhe: 3.278 ± 0.07	5.825LeuGly: 5.825 ± 0.091	1.061LeuHis: 1.061 ± 0.036	7.94LeuIle: 7.94 ± 0.118	9.465LeuLys: 9.465 ± 0.114	7.161LeuLeu: 7.161 ± 0.119	2.22LeuMet: 2.22 ± 0.055	6.353LeuAsn: 6.353 ± 0.099	2.623LeuPro: 2.623 ± 0.063	2.201LeuGln: 2.201 ± 0.055	2.944LeuArg: 2.944 ± 0.071	6.107LeuSer: 6.107 ± 0.091	4.351LeuThr: 4.351 ± 0.085	5.176LeuVal: 5.176 ± 0.088	0.525LeuTrp: 0.525 ± 0.026	3.219LeuTyr: 3.219 ± 0.073	0.0LeuXaa: 0.0 ± 0.0
Met	1.745MetAla: 1.745 ± 0.052	0.305MetCys: 0.305 ± 0.02	1.55MetAsp: 1.55 ± 0.046	1.912MetGlu: 1.912 ± 0.051	1.078MetPhe: 1.078 ± 0.035	1.875MetGly: 1.875 ± 0.056	0.311MetHis: 0.311 ± 0.02	2.175MetIle: 2.175 ± 0.056	2.672MetLys: 2.672 ± 0.073	2.206MetLeu: 2.206 ± 0.051	0.648MetMet: 0.648 ± 0.032	1.806MetAsn: 1.806 ± 0.053	0.813MetPro: 0.813 ± 0.033	0.639MetGln: 0.639 ± 0.034	0.868MetArg: 0.868 ± 0.032	1.701MetSer: 1.701 ± 0.043	1.175MetThr: 1.175 ± 0.039	1.435MetVal: 1.435 ± 0.045	0.146MetTrp: 0.146 ± 0.013	0.949MetTyr: 0.949 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.014AsnAla: 3.014 ± 0.065	0.853AsnCys: 0.853 ± 0.033	3.411AsnAsp: 3.411 ± 0.081	4.595AsnGlu: 4.595 ± 0.077	2.821AsnPhe: 2.821 ± 0.064	3.642AsnGly: 3.642 ± 0.07	0.862AsnHis: 0.862 ± 0.034	7.975AsnIle: 7.975 ± 0.11	7.579AsnLys: 7.579 ± 0.119	5.829AsnLeu: 5.829 ± 0.112	1.8AsnMet: 1.8 ± 0.048	5.326AsnAsn: 5.326 ± 0.102	2.106AsnPro: 2.106 ± 0.06	1.374AsnGln: 1.374 ± 0.046	1.999AsnArg: 1.999 ± 0.048	4.212AsnSer: 4.212 ± 0.078	3.202AsnThr: 3.202 ± 0.079	4.217AsnVal: 4.217 ± 0.074	0.473AsnTrp: 0.473 ± 0.026	2.896AsnTyr: 2.896 ± 0.071	0.0AsnXaa: 0.0 ± 0.0
Pro	1.304ProAla: 1.304 ± 0.05	0.368ProCys: 0.368 ± 0.023	1.221ProAsp: 1.221 ± 0.042	1.995ProGlu: 1.995 ± 0.053	1.312ProPhe: 1.312 ± 0.039	1.623ProGly: 1.623 ± 0.055	0.436ProHis: 0.436 ± 0.024	2.855ProIle: 2.855 ± 0.062	2.504ProLys: 2.504 ± 0.059	2.313ProLeu: 2.313 ± 0.057	0.722ProMet: 0.722 ± 0.03	1.689ProAsn: 1.689 ± 0.059	0.619ProPro: 0.619 ± 0.033	0.768ProGln: 0.768 ± 0.032	0.866ProArg: 0.866 ± 0.039	1.823ProSer: 1.823 ± 0.051	1.5ProThr: 1.5 ± 0.049	1.777ProVal: 1.777 ± 0.053	0.201ProTrp: 0.201 ± 0.017	1.247ProTyr: 1.247 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	1.239GlnAla: 1.239 ± 0.047	0.321GlnCys: 0.321 ± 0.022	1.29GlnAsp: 1.29 ± 0.048	1.742GlnGlu: 1.742 ± 0.052	0.99GlnPhe: 0.99 ± 0.038	1.489GlnGly: 1.489 ± 0.045	0.301GlnHis: 0.301 ± 0.022	2.163GlnIle: 2.163 ± 0.056	2.24GlnLys: 2.24 ± 0.06	2.051GlnLeu: 2.051 ± 0.055	0.641GlnMet: 0.641 ± 0.033	1.688GlnAsn: 1.688 ± 0.041	0.534GlnPro: 0.534 ± 0.029	0.794GlnGln: 0.794 ± 0.04	0.868GlnArg: 0.868 ± 0.039	1.387GlnSer: 1.387 ± 0.048	1.028GlnThr: 1.028 ± 0.039	1.549GlnVal: 1.549 ± 0.041	0.219GlnTrp: 0.219 ± 0.019	1.108GlnTyr: 1.108 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	1.763ArgAla: 1.763 ± 0.045	0.427ArgCys: 0.427 ± 0.027	1.815ArgAsp: 1.815 ± 0.053	2.942ArgGlu: 2.942 ± 0.064	1.335ArgPhe: 1.335 ± 0.043	1.954ArgGly: 1.954 ± 0.054	0.485ArgHis: 0.485 ± 0.026	3.117ArgIle: 3.117 ± 0.07	3.213ArgLys: 3.213 ± 0.067	2.712ArgLeu: 2.712 ± 0.06	0.937ArgMet: 0.937 ± 0.035	2.05ArgAsn: 2.05 ± 0.056	0.811ArgPro: 0.811 ± 0.036	0.951ArgGln: 0.951 ± 0.034	1.406ArgArg: 1.406 ± 0.048	1.489ArgSer: 1.489 ± 0.048	1.5ArgThr: 1.5 ± 0.045	2.189ArgVal: 2.189 ± 0.056	0.224ArgTrp: 0.224 ± 0.019	1.408ArgTyr: 1.408 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	3.086SerAla: 3.086 ± 0.075	0.686SerCys: 0.686 ± 0.029	2.949SerAsp: 2.949 ± 0.061	3.816SerGlu: 3.816 ± 0.067	2.794SerPhe: 2.794 ± 0.065	4.022SerGly: 4.022 ± 0.081	0.902SerHis: 0.902 ± 0.037	6.867SerIle: 6.867 ± 0.107	6.358SerLys: 6.358 ± 0.089	5.555SerLeu: 5.555 ± 0.089	1.661SerMet: 1.661 ± 0.046	4.147SerAsn: 4.147 ± 0.088	1.668SerPro: 1.668 ± 0.052	1.558SerGln: 1.558 ± 0.037	1.981SerArg: 1.981 ± 0.053	4.318SerSer: 4.318 ± 0.097	3.072SerThr: 3.072 ± 0.074	3.753SerVal: 3.753 ± 0.08	0.424SerTrp: 0.424 ± 0.023	2.516SerTyr: 2.516 ± 0.065	0.0SerXaa: 0.0 ± 0.0
Thr	2.745ThrAla: 2.745 ± 0.071	0.57ThrCys: 0.57 ± 0.026	2.251ThrAsp: 2.251 ± 0.055	2.962ThrGlu: 2.962 ± 0.063	2.103ThrPhe: 2.103 ± 0.048	3.46ThrGly: 3.46 ± 0.067	0.746ThrHis: 0.746 ± 0.027	5.172ThrIle: 5.172 ± 0.086	4.051ThrLys: 4.051 ± 0.077	4.657ThrLeu: 4.657 ± 0.091	1.126ThrMet: 1.126 ± 0.038	2.85ThrAsn: 2.85 ± 0.068	1.783ThrPro: 1.783 ± 0.051	1.063ThrGln: 1.063 ± 0.042	1.578ThrArg: 1.578 ± 0.048	3.158ThrSer: 3.158 ± 0.065	2.591ThrThr: 2.591 ± 0.067	3.396ThrVal: 3.396 ± 0.066	0.363ThrTrp: 0.363 ± 0.022	1.909ThrTyr: 1.909 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	3.661ValAla: 3.661 ± 0.072	0.906ValCys: 0.906 ± 0.04	3.842ValAsp: 3.842 ± 0.074	4.275ValGlu: 4.275 ± 0.078	2.821ValPhe: 2.821 ± 0.069	4.275ValGly: 4.275 ± 0.096	0.853ValHis: 0.853 ± 0.034	6.288ValIle: 6.288 ± 0.085	5.91ValLys: 5.91 ± 0.09	5.95ValLeu: 5.95 ± 0.097	1.561ValMet: 1.561 ± 0.051	3.888ValAsn: 3.888 ± 0.084	2.059ValPro: 2.059 ± 0.064	1.581ValGln: 1.581 ± 0.05	1.958ValArg: 1.958 ± 0.049	4.291ValSer: 4.291 ± 0.077	3.268ValThr: 3.268 ± 0.075	4.669ValVal: 4.669 ± 0.087	0.346ValTrp: 0.346 ± 0.022	2.318ValTyr: 2.318 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.326TrpAla: 0.326 ± 0.026	0.095TrpCys: 0.095 ± 0.011	0.363TrpAsp: 0.363 ± 0.023	0.409TrpGlu: 0.409 ± 0.022	0.307TrpPhe: 0.307 ± 0.021	0.452TrpGly: 0.452 ± 0.025	0.138TrpHis: 0.138 ± 0.015	0.656TrpIle: 0.656 ± 0.033	0.515TrpLys: 0.515 ± 0.026	0.562TrpLeu: 0.562 ± 0.03	0.17TrpMet: 0.17 ± 0.016	0.441TrpAsn: 0.441 ± 0.029	0.114TrpPro: 0.114 ± 0.013	0.195TrpGln: 0.195 ± 0.016	0.228TrpArg: 0.228 ± 0.019	0.363TrpSer: 0.363 ± 0.027	0.305TrpThr: 0.305 ± 0.022	0.372TrpVal: 0.372 ± 0.022	0.068TrpTrp: 0.068 ± 0.009	0.295TrpTyr: 0.295 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.863TyrAla: 1.863 ± 0.052	0.534TyrCys: 0.534 ± 0.029	2.422TyrAsp: 2.422 ± 0.061	2.709TyrGlu: 2.709 ± 0.073	1.981TyrPhe: 1.981 ± 0.059	2.459TyrGly: 2.459 ± 0.063	0.505TyrHis: 0.505 ± 0.027	4.232TyrIle: 4.232 ± 0.092	4.282TyrLys: 4.282 ± 0.095	3.445TyrLeu: 3.445 ± 0.071	1.06TyrMet: 1.06 ± 0.043	3.116TyrAsn: 3.116 ± 0.079	1.221TyrPro: 1.221 ± 0.045	0.738TyrGln: 0.738 ± 0.033	1.38TyrArg: 1.38 ± 0.043	2.774TyrSer: 2.774 ± 0.055	2.052TyrThr: 2.052 ± 0.058	2.322TyrVal: 2.322 ± 0.064	0.293TyrTrp: 0.293 ± 0.021	1.774TyrTyr: 1.774 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2552 proteins (754814 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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