Alicyclobacillus ferrooxydans
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4142 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P9GNW6|A0A0P9GNW6_9BACL FAD-binding PCMH-type domain-containing protein OS=Alicyclobacillus ferrooxydans OX=471514 GN=AN477_18095 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 9.87 VTIEE6 pKa = 4.09 TSGQKK11 pKa = 10.65 YY12 pKa = 10.09 SVTLEE17 pKa = 4.23 MDD19 pKa = 3.61 DD20 pKa = 3.35 KK21 pKa = 11.25 TYY23 pKa = 11.06 VANHH27 pKa = 6.38 EE28 pKa = 4.22 LTSYY32 pKa = 10.45 GAHH35 pKa = 6.31 QIGGVNFDD43 pKa = 3.55 EE44 pKa = 4.42 QDD46 pKa = 2.93 FDD48 pKa = 5.46 RR49 pKa = 11.84 EE50 pKa = 4.16 LEE52 pKa = 4.23 YY53 pKa = 10.86 IMSDD57 pKa = 3.04 IEE59 pKa = 4.22 SQAYY63 pKa = 8.46 EE64 pKa = 3.93 LAYY67 pKa = 10.71 YY68 pKa = 10.28 DD69 pKa = 3.39 AEE71 pKa = 4.06 TRR73 pKa = 11.84 EE74 pKa = 4.16 RR75 pKa = 11.84 SEE77 pKa = 4.06 SDD79 pKa = 2.89 EE80 pKa = 5.63 DD81 pKa = 4.92 GDD83 pKa = 5.47 DD84 pKa = 3.59 EE85 pKa = 4.95
Molecular weight: 9.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.656
ProMoST 3.961
Dawson 3.821
Bjellqvist 4.024
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.567
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.101
Thurlkill 3.694
EMBOSS 3.732
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.882
Protein with the highest isoelectric point:
>tr|A0A0P9GQL5|A0A0P9GQL5_9BACL Acetyltransferase OS=Alicyclobacillus ferrooxydans OX=471514 GN=AN477_14060 PE=4 SV=1
MM1 pKa = 7.36 FVPIWAMRR9 pKa = 11.84 HH10 pKa = 5.03 FCSLWTIQRR19 pKa = 11.84 KK20 pKa = 8.11 IRR22 pKa = 11.84 LYY24 pKa = 10.32 RR25 pKa = 11.84 RR26 pKa = 11.84 AGLRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 PRR34 pKa = 11.84 SVRR37 pKa = 11.84 TVLTDD42 pKa = 3.18 SRR44 pKa = 11.84 AKK46 pKa = 10.76 ARR48 pKa = 11.84 TFYY51 pKa = 11.15 ALSFAFYY58 pKa = 10.8 FEE60 pKa = 5.21 FEE62 pKa = 4.23 SCDD65 pKa = 3.31 VIEE68 pKa = 4.53 NVRR71 pKa = 11.84 QRR73 pKa = 11.84 TTEE76 pKa = 3.8 NEE78 pKa = 4.17 RR79 pKa = 11.84 QGSNAKK85 pKa = 9.85 QKK87 pKa = 10.87 EE88 pKa = 4.31 DD89 pKa = 3.82 VEE91 pKa = 4.56 GSPAACPLHH100 pKa = 6.8 AWVKK104 pKa = 8.47 WRR106 pKa = 11.84 CTGGPP111 pKa = 3.35
Molecular weight: 13.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.545
IPC_protein 10.379
Toseland 10.555
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.804
Grimsley 10.716
Solomon 10.789
Lehninger 10.745
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.54
IPC_peptide 10.789
IPC2_peptide 9.706
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4142
0
4142
1212945
26
1718
292.8
32.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.928 ± 0.041
0.824 ± 0.011
5.022 ± 0.032
5.916 ± 0.043
4.015 ± 0.03
7.823 ± 0.033
2.429 ± 0.018
5.86 ± 0.033
3.962 ± 0.029
9.938 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.713 ± 0.018
3.386 ± 0.026
4.357 ± 0.024
4.053 ± 0.032
5.751 ± 0.034
6.328 ± 0.035
5.841 ± 0.032
8.371 ± 0.031
1.444 ± 0.018
3.038 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here