Alginatibacterium sediminis
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3984 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A420EGM1|A0A420EGM1_9ALTE 3-isopropylmalate dehydrogenase OS=Alginatibacterium sediminis OX=2164068 GN=leuB PE=3 SV=1
MM1 pKa = 7.78 YY2 pKa = 9.6 EE3 pKa = 3.56 IAKK6 pKa = 10.25 ILGLTVLLSSTAKK19 pKa = 9.88 ADD21 pKa = 3.56 NVEE24 pKa = 4.4 VYY26 pKa = 9.11 VTDD29 pKa = 5.62 LLDD32 pKa = 3.68 NTQNGYY38 pKa = 10.23 CLDD41 pKa = 3.43 IAKK44 pKa = 10.67 GKK46 pKa = 10.26 GADD49 pKa = 3.65 ANPADD54 pKa = 4.02 GMQAHH59 pKa = 6.27 TCYY62 pKa = 10.81 SPLGEE67 pKa = 4.24 VLVDD71 pKa = 3.84 QAFDD75 pKa = 3.5 PAKK78 pKa = 10.4 FEE80 pKa = 4.26 EE81 pKa = 5.16 GVLYY85 pKa = 10.02 MPEE88 pKa = 4.48 FDD90 pKa = 3.96 VCMQASSTEE99 pKa = 3.97 IGASPEE105 pKa = 3.89 LTTCDD110 pKa = 3.34 GSEE113 pKa = 3.91 AQSWVFDD120 pKa = 4.03 GEE122 pKa = 4.49 GTIAPAAAPEE132 pKa = 4.11 MCLTLTGDD140 pKa = 3.45 TRR142 pKa = 11.84 TGRR145 pKa = 11.84 SDD147 pKa = 3.6 EE148 pKa = 4.24 NQMKK152 pKa = 10.75 AMTLEE157 pKa = 4.19 ACSVDD162 pKa = 3.94 LAPVQMWSNRR172 pKa = 11.84 TADD175 pKa = 3.14
Molecular weight: 18.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.643
ProMoST 3.948
Dawson 3.834
Bjellqvist 4.024
Wikipedia 3.745
Rodwell 3.681
Grimsley 3.554
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.961
Patrickios 1.036
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A420E5M6|A0A420E5M6_9ALTE Dihydroxyacetone kinase subunit DhaK OS=Alginatibacterium sediminis OX=2164068 GN=dhaK PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.39 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3984
0
3984
1305717
16
5264
327.7
36.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.757 ± 0.04
1.108 ± 0.015
5.454 ± 0.029
5.923 ± 0.032
4.264 ± 0.027
6.605 ± 0.039
2.214 ± 0.02
6.461 ± 0.032
4.819 ± 0.03
10.966 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.02
4.163 ± 0.032
3.755 ± 0.025
5.324 ± 0.043
4.608 ± 0.027
7.391 ± 0.036
4.62 ± 0.027
6.789 ± 0.032
1.264 ± 0.018
2.988 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here