Dentipellis sp. KUC8613
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14287 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B1QFU5|A0A5B1QFU5_9AGAM Uncharacterized protein OS=Dentipellis sp. KUC8613 OX=1883078 GN=DENSPDRAFT_833366 PE=4 SV=1
MM1 pKa = 7.34 FATLFTVTLLSAGAIKK17 pKa = 10.46 GALAGLAINSPTLVQCEE34 pKa = 4.09 DD35 pKa = 3.6 AHH37 pKa = 6.88 VSWASTKK44 pKa = 10.43 GPYY47 pKa = 10.08 NLIVTPGDD55 pKa = 3.95 EE56 pKa = 4.39 PCGDD60 pKa = 4.68 AIVDD64 pKa = 4.35 LGDD67 pKa = 3.93 HH68 pKa = 7.39 DD69 pKa = 4.64 GTTMTYY75 pKa = 10.74 NVALPAGQKK84 pKa = 10.12 VLLSLQDD91 pKa = 3.73 ANGDD95 pKa = 3.93 EE96 pKa = 4.53 AWSQEE101 pKa = 3.93 LTVQPGSNSACLPASLQPSSSIATLSAASSSVKK134 pKa = 9.85 FASSSAPFSLSSTVVPLTAVTPATTISALALPSDD168 pKa = 4.25 SAAAAPSDD176 pKa = 4.17 TIGGDD181 pKa = 3.57 DD182 pKa = 3.63 SDD184 pKa = 4.41 APSVVGGAANAASNPFSGAPAMHH207 pKa = 6.31 QLYY210 pKa = 8.28 TPAMFLSALGAAFFVALL227 pKa = 4.35
Molecular weight: 22.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.961
Patrickios 0.896
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|A0A5B1RER3|A0A5B1RER3_9AGAM Uncharacterized protein OS=Dentipellis sp. KUC8613 OX=1883078 GN=DENSPDRAFT_887317 PE=4 SV=1
MM1 pKa = 6.33 QTRR4 pKa = 11.84 LRR6 pKa = 11.84 LVLSLSRR13 pKa = 11.84 CLVPSLPPSRR23 pKa = 11.84 PLRR26 pKa = 11.84 AVAPPHH32 pKa = 5.43 TLVVSSHH39 pKa = 5.32 HH40 pKa = 6.18 RR41 pKa = 11.84 AVILSRR47 pKa = 11.84 HH48 pKa = 5.44 RR49 pKa = 11.84 ASLAHH54 pKa = 6.85 LPRR57 pKa = 11.84 PASTLVTSSSRR68 pKa = 11.84 RR69 pKa = 11.84 RR70 pKa = 11.84 ILVCRR75 pKa = 11.84 HH76 pKa = 5.03 RR77 pKa = 11.84 TLVAPSFPRR86 pKa = 11.84 AVALSRR92 pKa = 11.84 SSRR95 pKa = 11.84 APPAVSMLSPRR106 pKa = 11.84 RR107 pKa = 11.84 VVVASSRR114 pKa = 11.84 PAPPALLVPLPPLQPPPCRR133 pKa = 11.84 APHH136 pKa = 6.17 PRR138 pKa = 11.84 VAPSPSVASSRR149 pKa = 11.84 PCRR152 pKa = 11.84 ARR154 pKa = 11.84 RR155 pKa = 11.84 VRR157 pKa = 11.84 VAPVASVSRR166 pKa = 11.84 PSRR169 pKa = 11.84 PSRR172 pKa = 11.84 PSRR175 pKa = 11.84 PRR177 pKa = 11.84 LAVAPILRR185 pKa = 11.84 PSRR188 pKa = 11.84 RR189 pKa = 11.84 SRR191 pKa = 11.84 HH192 pKa = 4.6 RR193 pKa = 11.84 ARR195 pKa = 11.84 PRR197 pKa = 11.84 RR198 pKa = 11.84 RR199 pKa = 11.84 AVAPVVPLCRR209 pKa = 11.84 RR210 pKa = 11.84 ARR212 pKa = 11.84 RR213 pKa = 11.84 AVAALPLSALAPPSRR228 pKa = 11.84 PSHH231 pKa = 5.54 AHH233 pKa = 5.6 HH234 pKa = 6.81 VPSAHH239 pKa = 6.28 AQPSRR244 pKa = 11.84 PSRR247 pKa = 11.84 VRR249 pKa = 11.84 RR250 pKa = 11.84 ARR252 pKa = 11.84 HH253 pKa = 5.49 APRR256 pKa = 11.84 SVPRR260 pKa = 11.84 HH261 pKa = 4.69 ARR263 pKa = 11.84 RR264 pKa = 11.84 ARR266 pKa = 11.84 RR267 pKa = 11.84 TPFASRR273 pKa = 11.84 PSRR276 pKa = 11.84 HH277 pKa = 5.38 RR278 pKa = 11.84 ASRR281 pKa = 11.84 RR282 pKa = 11.84 LRR284 pKa = 11.84 AVVPSRR290 pKa = 11.84 PSRR293 pKa = 11.84 VRR295 pKa = 11.84 RR296 pKa = 11.84 AVATFAPLSAIAPPSRR312 pKa = 11.84 PAHH315 pKa = 5.73 AHH317 pKa = 5.72 HH318 pKa = 7.09 APGAIAPVAAFAPSRR333 pKa = 11.84 RR334 pKa = 11.84 RR335 pKa = 11.84 AVAPVARR342 pKa = 11.84 LSRR345 pKa = 11.84 PIRR348 pKa = 11.84 AAGVMPSRR356 pKa = 11.84 RR357 pKa = 11.84 RR358 pKa = 11.84 HH359 pKa = 4.87 ALARR363 pKa = 11.84 PARR366 pKa = 11.84 PRR368 pKa = 11.84 AHH370 pKa = 6.58 FVPVLHH376 pKa = 6.7 LHH378 pKa = 5.68 MDD380 pKa = 3.97 VYY382 pKa = 11.68 VLLL385 pKa = 4.72
Molecular weight: 42.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.164
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14287
0
14287
5785676
49
5066
405.0
44.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.826 ± 0.026
1.432 ± 0.012
5.317 ± 0.019
5.521 ± 0.025
3.516 ± 0.014
6.459 ± 0.023
2.752 ± 0.011
4.3 ± 0.016
4.016 ± 0.02
9.138 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.009
2.977 ± 0.013
7.494 ± 0.037
3.558 ± 0.015
7.051 ± 0.031
8.549 ± 0.028
5.866 ± 0.014
6.346 ± 0.016
1.405 ± 0.008
2.473 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here