Desulfobacca acetoxidans (strain ATCC 700848 / DSM 11109 / ASRB2)
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2859 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2NFD9|F2NFD9_DESAR Uncharacterized protein OS=Desulfobacca acetoxidans (strain ATCC 700848 / DSM 11109 / ASRB2) OX=880072 GN=Desac_2231 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.31 KK3 pKa = 9.76 NLSIILILWIVALAAGTAQAFTFNDD28 pKa = 3.21 VNIWAGSGSNSAGLVIHH45 pKa = 6.67 WSAPEE50 pKa = 4.06 VYY52 pKa = 10.31 NNTSMPAPIADD63 pKa = 3.3 ISVAWGYY70 pKa = 11.43 KK71 pKa = 9.78 FDD73 pKa = 3.87 GTANAEE79 pKa = 4.19 TMMVALAAADD89 pKa = 3.51 PRR91 pKa = 11.84 LYY93 pKa = 10.86 LLAGGQPGLGMAILGIGYY111 pKa = 9.34 DD112 pKa = 3.79 LNNNGVFGLSDD123 pKa = 3.72 GSTTYY128 pKa = 9.76 TAADD132 pKa = 3.79 FANGILADD140 pKa = 3.96 QPYY143 pKa = 10.86 GDD145 pKa = 5.63 ADD147 pKa = 3.89 TLLPTDD153 pKa = 4.81 PGDD156 pKa = 4.09 LYY158 pKa = 10.57 WGGWYY163 pKa = 10.15 GPNWEE168 pKa = 4.71 LWHH171 pKa = 5.81 EE172 pKa = 4.07 QGGNGGFTSAPDD184 pKa = 3.66 RR185 pKa = 11.84 GSDD188 pKa = 3.97 LYY190 pKa = 9.37 WTGSFFSGSHH200 pKa = 5.94 GEE202 pKa = 3.81 WDD204 pKa = 3.97 FSGLGITSLSLEE216 pKa = 4.6 DD217 pKa = 4.31 GSWVGWSVAAGGLDD231 pKa = 3.04 MGDD234 pKa = 3.38 PMGEE238 pKa = 4.05 GTLAWMNHH246 pKa = 4.55 KK247 pKa = 9.65 QAPGEE252 pKa = 3.91 PLAAPVPITGAVWLLGSGLLRR273 pKa = 11.84 LIGICRR279 pKa = 11.84 RR280 pKa = 11.84 CASS283 pKa = 3.41
Molecular weight: 29.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 4.037
IPC_protein 4.024
Toseland 3.808
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.973
Rodwell 3.859
Grimsley 3.719
Solomon 4.024
Lehninger 3.973
Nozaki 4.139
DTASelect 4.406
Thurlkill 3.872
EMBOSS 3.986
Sillero 4.151
Patrickios 1.138
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.012
Protein with the highest isoelectric point:
>tr|F2NH63|F2NH63_DESAR Methyl-viologen-reducing hydrogenase delta subunit OS=Desulfobacca acetoxidans (strain ATCC 700848 / DSM 11109 / ASRB2) OX=880072 GN=Desac_1039 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.08 QPHH8 pKa = 5.37 NLKK11 pKa = 10.35 RR12 pKa = 11.84 ARR14 pKa = 11.84 TFGFLTRR21 pKa = 11.84 MSTRR25 pKa = 11.84 GGRR28 pKa = 11.84 LVIKK32 pKa = 10.37 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2859
0
2859
919224
30
3125
321.5
35.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.986 ± 0.047
1.22 ± 0.022
4.76 ± 0.027
6.304 ± 0.042
4.124 ± 0.032
7.676 ± 0.066
2.093 ± 0.022
6.222 ± 0.04
4.589 ± 0.043
11.506 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.239 ± 0.023
3.181 ± 0.033
5.119 ± 0.031
4.271 ± 0.029
6.444 ± 0.039
5.196 ± 0.031
4.913 ± 0.032
6.838 ± 0.042
1.275 ± 0.021
3.043 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here