Desulfobacca acetoxidans (strain ATCC 700848 / DSM 11109 / ASRB2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Desulfobacca; Desulfobacca acetoxidans

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F2NFD9|F2NFD9_DESAR Uncharacterized protein OS=Desulfobacca acetoxidans (strain ATCC 700848 / DSM 11109 / ASRB2) OX=880072 GN=Desac_2231 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.31KK3 pKa = 9.76NLSIILILWIVALAAGTAQAFTFNDD28 pKa = 3.21VNIWAGSGSNSAGLVIHH45 pKa = 6.67WSAPEE50 pKa = 4.06VYY52 pKa = 10.31NNTSMPAPIADD63 pKa = 3.3ISVAWGYY70 pKa = 11.43KK71 pKa = 9.78FDD73 pKa = 3.87GTANAEE79 pKa = 4.19TMMVALAAADD89 pKa = 3.51PRR91 pKa = 11.84LYY93 pKa = 10.86LLAGGQPGLGMAILGIGYY111 pKa = 9.34DD112 pKa = 3.79LNNNGVFGLSDD123 pKa = 3.72GSTTYY128 pKa = 9.76TAADD132 pKa = 3.79FANGILADD140 pKa = 3.96QPYY143 pKa = 10.86GDD145 pKa = 5.63ADD147 pKa = 3.89TLLPTDD153 pKa = 4.81PGDD156 pKa = 4.09LYY158 pKa = 10.57WGGWYY163 pKa = 10.15GPNWEE168 pKa = 4.71LWHH171 pKa = 5.81EE172 pKa = 4.07QGGNGGFTSAPDD184 pKa = 3.66RR185 pKa = 11.84GSDD188 pKa = 3.97LYY190 pKa = 9.37WTGSFFSGSHH200 pKa = 5.94GEE202 pKa = 3.81WDD204 pKa = 3.97FSGLGITSLSLEE216 pKa = 4.6DD217 pKa = 4.31GSWVGWSVAAGGLDD231 pKa = 3.04MGDD234 pKa = 3.38PMGEE238 pKa = 4.05GTLAWMNHH246 pKa = 4.55KK247 pKa = 9.65QAPGEE252 pKa = 3.91PLAAPVPITGAVWLLGSGLLRR273 pKa = 11.84LIGICRR279 pKa = 11.84RR280 pKa = 11.84CASS283 pKa = 3.41

Molecular weight:
29.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F2NH63|F2NH63_DESAR Methyl-viologen-reducing hydrogenase delta subunit OS=Desulfobacca acetoxidans (strain ATCC 700848 / DSM 11109 / ASRB2) OX=880072 GN=Desac_1039 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.08QPHH8 pKa = 5.37NLKK11 pKa = 10.35RR12 pKa = 11.84ARR14 pKa = 11.84TFGFLTRR21 pKa = 11.84MSTRR25 pKa = 11.84GGRR28 pKa = 11.84LVIKK32 pKa = 10.37RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2859

0

2859

919224

30

3125

321.5

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.986 ± 0.047

1.22 ± 0.022

4.76 ± 0.027

6.304 ± 0.042

4.124 ± 0.032

7.676 ± 0.066

2.093 ± 0.022

6.222 ± 0.04

4.589 ± 0.043

11.506 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.023

3.181 ± 0.033

5.119 ± 0.031

4.271 ± 0.029

6.444 ± 0.039

5.196 ± 0.031

4.913 ± 0.032

6.838 ± 0.042

1.275 ± 0.021

3.043 ± 0.028

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski