Methanolobus tindarius DSM 2278

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanolobus; Methanolobus tindarius

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2912 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9DN22|W9DN22_METTI Uncharacterized protein OS=Methanolobus tindarius DSM 2278 OX=1090322 GN=MettiDRAFT_0754 PE=4 SV=1
MM1 pKa = 7.16VKK3 pKa = 10.1EE4 pKa = 4.37KK5 pKa = 10.55VHH7 pKa = 7.86IYY9 pKa = 10.32IIFPTFLCLVMSLSTPAYY27 pKa = 9.29AAGDD31 pKa = 3.4ITAVRR36 pKa = 11.84EE37 pKa = 3.91ISNMNVTPGEE47 pKa = 4.23TFNVSVTLKK56 pKa = 10.27MNADD60 pKa = 3.81VIGPLLRR67 pKa = 11.84EE68 pKa = 4.07TLPDD72 pKa = 2.77GWTIGSLDD80 pKa = 3.54NDD82 pKa = 3.21GSLFNVNTTEE92 pKa = 4.21WAWVSMLQSGEE103 pKa = 4.33SKK105 pKa = 8.74TVEE108 pKa = 4.05YY109 pKa = 11.03SVMVPDD115 pKa = 4.38DD116 pKa = 3.94TDD118 pKa = 4.84DD119 pKa = 3.62GVYY122 pKa = 10.12TISGYY127 pKa = 8.99VAPEE131 pKa = 4.0AYY133 pKa = 10.41NYY135 pKa = 8.57ITVLGDD141 pKa = 3.38TQVTVSSEE149 pKa = 3.88TSTPEE154 pKa = 3.7VIADD158 pKa = 4.01FTANITSGKK167 pKa = 10.02VPLTVKK173 pKa = 9.65FTDD176 pKa = 3.1KK177 pKa = 10.34STNATSWQWDD187 pKa = 3.48FDD189 pKa = 4.13GDD191 pKa = 3.66NSIDD195 pKa = 3.67STEE198 pKa = 3.92QNPVYY203 pKa = 9.66TYY205 pKa = 10.86QSAGTYY211 pKa = 6.62TVKK214 pKa = 10.87LISSSNSDD222 pKa = 2.91SDD224 pKa = 4.71VEE226 pKa = 4.48TKK228 pKa = 10.08TDD230 pKa = 3.88YY231 pKa = 10.09ITIKK235 pKa = 10.64SGGGSSGTSLKK246 pKa = 10.83ADD248 pKa = 3.39FTVNITSGQAPLSVQFTDD266 pKa = 3.42QSSGNVSAWQWDD278 pKa = 3.63FDD280 pKa = 4.16GDD282 pKa = 3.96NVIDD286 pKa = 4.0STEE289 pKa = 3.75QDD291 pKa = 3.3PVHH294 pKa = 6.79TYY296 pKa = 9.56TEE298 pKa = 4.2NGSYY302 pKa = 7.97TVKK305 pKa = 10.88LIVYY309 pKa = 9.79DD310 pKa = 4.39DD311 pKa = 4.91DD312 pKa = 5.17SNSDD316 pKa = 3.31ALTEE320 pKa = 3.91VAYY323 pKa = 8.97ITVSDD328 pKa = 4.33EE329 pKa = 4.46EE330 pKa = 6.21DD331 pKa = 3.66DD332 pKa = 4.89DD333 pKa = 5.27SYY335 pKa = 12.22NSYY338 pKa = 10.67EE339 pKa = 4.01ATSVSLSATIIPSISIEE356 pKa = 4.16VTPGTMNFGTLSAGEE371 pKa = 4.18VSDD374 pKa = 4.04EE375 pKa = 4.13HH376 pKa = 6.24TLHH379 pKa = 6.67IKK381 pKa = 10.63NKK383 pKa = 8.48GATDD387 pKa = 3.57ATVTAQVTDD396 pKa = 3.66VAKK399 pKa = 10.64DD400 pKa = 3.61LYY402 pKa = 11.52VDD404 pKa = 4.05GLMLNSGTWDD414 pKa = 3.66GYY416 pKa = 9.51SNVVEE421 pKa = 4.47SEE423 pKa = 4.09TTEE426 pKa = 4.15TVQASLNVPDD436 pKa = 4.95DD437 pKa = 4.04FIGIGSMEE445 pKa = 4.22GKK447 pKa = 10.01LVFWAEE453 pKa = 3.78ISS455 pKa = 3.43

Molecular weight: 49.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9DP00|W9DP00_METTI Uncharacterized protein OS=Methanolobus tindarius DSM 2278 OX=1090322 GN=MettiDRAFT_0155 PE=4 SV=1
MM1 pKa = 7.14SHH3 pKa = 5.42NTKK6 pKa = 9.9GQKK9 pKa = 8.31TRR11 pKa = 11.84LAKK14 pKa = 10.28AHH16 pKa = 5.91RR17 pKa = 11.84QNHH20 pKa = 5.7RR21 pKa = 11.84VPTWVIIKK29 pKa = 8.85TGRR32 pKa = 11.84KK33 pKa = 8.65VVSHH37 pKa = 6.6PKK39 pKa = 8.12RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.72WRR44 pKa = 11.84RR45 pKa = 11.84SSLDD49 pKa = 3.23VKK51 pKa = 11.09

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2912

0

2912

910742

29

3072

312.8

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.761 ± 0.046

1.176 ± 0.021

6.396 ± 0.042

7.686 ± 0.056

4.079 ± 0.029

6.871 ± 0.044

1.786 ± 0.02

8.265 ± 0.043

6.262 ± 0.055

8.808 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.025

4.709 ± 0.048

3.565 ± 0.028

2.514 ± 0.024

3.854 ± 0.035

7.125 ± 0.047

5.529 ± 0.045

7.184 ± 0.041

0.879 ± 0.016

3.56 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski