Thielaviopsis punctulata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Microascales; Ceratocystidaceae; Thielaviopsis

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4ZKS4|A0A0F4ZKS4_9PEZI EXS domain-containing protein OS=Thielaviopsis punctulata OX=72032 GN=TD95_005055 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.3FPSTAALFLALSGFALAQDD21 pKa = 4.05VSSTPSTSCVSGAACATAQTTMSSVVSSVTASTTTTAAAATSTHH65 pKa = 7.37DD66 pKa = 4.75DD67 pKa = 4.09DD68 pKa = 6.82HH69 pKa = 9.17DD70 pKa = 5.21DD71 pKa = 5.11DD72 pKa = 6.73HH73 pKa = 9.36DD74 pKa = 5.32DD75 pKa = 5.08DD76 pKa = 6.79HH77 pKa = 9.15DD78 pKa = 5.23DD79 pKa = 4.1DD80 pKa = 4.77HH81 pKa = 7.66AAGNGSLAPSPTEE94 pKa = 3.94SVGCEE99 pKa = 3.43PHH101 pKa = 6.71GDD103 pKa = 3.31HH104 pKa = 6.47WHH106 pKa = 6.86CEE108 pKa = 4.09APATAAAATGTASGSATSAGSVQEE132 pKa = 4.48TEE134 pKa = 4.02TATAGGDD141 pKa = 3.54KK142 pKa = 10.91VSVFAGALAIVILAII157 pKa = 4.28

Molecular weight: 15.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4ZFK2|A0A0F4ZFK2_9PEZI MI domain-containing protein OS=Thielaviopsis punctulata OX=72032 GN=TD95_002508 PE=3 SV=1
MM1 pKa = 7.16AQVLVSPSAADD12 pKa = 3.27SAANVAARR20 pKa = 11.84ALLNGASPRR29 pKa = 11.84PAPPPALSAPVPSIPRR45 pKa = 11.84RR46 pKa = 11.84PMTDD50 pKa = 3.2EE51 pKa = 3.52EE52 pKa = 4.17HH53 pKa = 6.41AALVKK58 pKa = 10.43AQRR61 pKa = 11.84AAKK64 pKa = 10.27AGVRR68 pKa = 11.84PPRR71 pKa = 11.84RR72 pKa = 11.84SHH74 pKa = 5.91SASEE78 pKa = 3.98FDD80 pKa = 5.71IEE82 pKa = 4.32QQMPNHH88 pKa = 6.87PPQPRR93 pKa = 11.84YY94 pKa = 10.38LYY96 pKa = 9.95TPVLAPTQASPISPLTPLTPGLPAVMEE123 pKa = 4.34LASPSTPTTPSTNTSLPPQPPMQVRR148 pKa = 11.84TARR151 pKa = 11.84PKK153 pKa = 9.39PKK155 pKa = 9.99KK156 pKa = 8.44PPRR159 pKa = 11.84RR160 pKa = 11.84AFSLMEE166 pKa = 3.99YY167 pKa = 9.79EE168 pKa = 4.6PVVHH172 pKa = 5.76THH174 pKa = 6.23RR175 pKa = 11.84PSNRR179 pKa = 11.84VSLLDD184 pKa = 4.19LPTDD188 pKa = 2.9IHH190 pKa = 6.54FAIFDD195 pKa = 3.88FLDD198 pKa = 5.16PIDD201 pKa = 4.22SVCFGLSSRR210 pKa = 11.84VFYY213 pKa = 9.93PIHH216 pKa = 6.57RR217 pKa = 11.84RR218 pKa = 11.84LHH220 pKa = 5.64GRR222 pKa = 11.84VSLASRR228 pKa = 11.84RR229 pKa = 11.84NGPNTLDD236 pKa = 3.87GPNPARR242 pKa = 11.84PPPRR246 pKa = 11.84SPGLVDD252 pKa = 4.15RR253 pKa = 11.84EE254 pKa = 4.41AIRR257 pKa = 11.84LDD259 pKa = 3.26GQVYY263 pKa = 9.81CRR265 pKa = 11.84KK266 pKa = 9.95CGTSRR271 pKa = 11.84CEE273 pKa = 3.79LHH275 pKa = 5.71SHH277 pKa = 5.35LRR279 pKa = 11.84EE280 pKa = 3.76WMGPDD285 pKa = 3.39RR286 pKa = 11.84EE287 pKa = 4.38YY288 pKa = 11.33CSVSRR293 pKa = 11.84KK294 pKa = 9.24FGKK297 pKa = 9.81KK298 pKa = 9.24APAGARR304 pKa = 11.84EE305 pKa = 4.19SCYY308 pKa = 10.35KK309 pKa = 10.5RR310 pKa = 11.84SPKK313 pKa = 10.29NPSACGRR320 pKa = 11.84HH321 pKa = 4.71TPRR324 pKa = 11.84MRR326 pKa = 11.84ASAPVSPKK334 pKa = 10.35LQAVQMAA341 pKa = 4.15

Molecular weight: 37.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5254

0

5254

2870050

15

4924

546.3

60.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.808 ± 0.036

1.097 ± 0.01

5.706 ± 0.024

6.038 ± 0.034

3.605 ± 0.022

6.619 ± 0.029

2.317 ± 0.013

4.659 ± 0.024

4.901 ± 0.028

8.603 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.014

3.631 ± 0.017

5.953 ± 0.037

3.911 ± 0.026

5.986 ± 0.026

8.393 ± 0.042

5.958 ± 0.025

6.393 ± 0.025

1.287 ± 0.011

2.63 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski