Yangia sp. CCB-MM3
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4896 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1PYL8|A0A1B1PYL8_9RHOB Uncharacterized protein OS=Yangia sp. CCB-MM3 OX=1792508 GN=AYJ57_24555 PE=4 SV=1
MM1 pKa = 7.53 AADD4 pKa = 4.83 FDD6 pKa = 4.6 DD7 pKa = 6.06 LDD9 pKa = 3.67 VLAQQEE15 pKa = 4.28 IAASEE20 pKa = 4.33 SATNGNGANSLRR32 pKa = 11.84 NLIEE36 pKa = 4.44 RR37 pKa = 11.84 GVLDD41 pKa = 3.61 EE42 pKa = 4.44 SGRR45 pKa = 11.84 EE46 pKa = 3.88 IGSEE50 pKa = 4.2 SASPAPPGEE59 pKa = 4.51 AGSGVLDD66 pKa = 3.48 IFVEE70 pKa = 4.68 DD71 pKa = 4.1 GLLPGIRR78 pKa = 11.84 SALEE82 pKa = 3.65 QYY84 pKa = 10.61 ISDD87 pKa = 4.31 IEE89 pKa = 4.24 LEE91 pKa = 4.69 GYY93 pKa = 7.24 QVRR96 pKa = 11.84 VVEE99 pKa = 4.52 FDD101 pKa = 3.42 GSAEE105 pKa = 3.95 EE106 pKa = 4.63 LRR108 pKa = 11.84 SEE110 pKa = 5.62 LEE112 pKa = 4.01 ARR114 pKa = 11.84 WLNDD118 pKa = 3.12 GLEE121 pKa = 4.36 GALFVGDD128 pKa = 5.33 LPVQYY133 pKa = 10.27 FKK135 pKa = 11.28 SYY137 pKa = 11.14 DD138 pKa = 3.52 GYY140 pKa = 11.31 DD141 pKa = 3.13 ADD143 pKa = 4.35 GQLVRR148 pKa = 11.84 YY149 pKa = 8.34 PHH151 pKa = 7.16 DD152 pKa = 4.61 LYY154 pKa = 11.61 FMDD157 pKa = 5.8 LDD159 pKa = 3.91 GSYY162 pKa = 11.13 SFNASGADD170 pKa = 3.29 AHH172 pKa = 6.95 VDD174 pKa = 3.14 GDD176 pKa = 4.01 GDD178 pKa = 4.05 VAPEE182 pKa = 4.06 IYY184 pKa = 10.23 VSRR187 pKa = 11.84 LAASQLGGLSSSSEE201 pKa = 4.11 TEE203 pKa = 4.34 LINSYY208 pKa = 8.55 FAKK211 pKa = 10.0 LHH213 pKa = 5.15 EE214 pKa = 4.25 VRR216 pKa = 11.84 AAGRR220 pKa = 11.84 QYY222 pKa = 9.76 QEE224 pKa = 3.53 RR225 pKa = 11.84 AIWFADD231 pKa = 3.77 NDD233 pKa = 4.06 WAPYY237 pKa = 9.57 TGDD240 pKa = 3.97 LEE242 pKa = 4.78 SLTGLYY248 pKa = 10.43 SEE250 pKa = 4.58 VTDD253 pKa = 4.53 IRR255 pKa = 11.84 ALDD258 pKa = 3.69 EE259 pKa = 4.31 TTVANYY265 pKa = 7.79 EE266 pKa = 3.84 AALQEE271 pKa = 4.31 DD272 pKa = 5.35 VEE274 pKa = 4.47 TLIQQIHH281 pKa = 6.0 AWSSGLSISSPGGGLVFSADD301 pKa = 3.51 VVDD304 pKa = 6.21 LNAQPAFINLFNCSSADD321 pKa = 3.4 FTQWSNLASTYY332 pKa = 10.88 VFLSDD337 pKa = 4.36 GVLNTVGSTKK347 pKa = 10.06 TGSMLNFADD356 pKa = 5.76 FYY358 pKa = 10.64 RR359 pKa = 11.84 PQSAGLTLGQSFLDD373 pKa = 3.65 WFEE376 pKa = 3.78 MHH378 pKa = 7.11 AAATDD383 pKa = 3.77 EE384 pKa = 4.79 PALDD388 pKa = 3.8 WKK390 pKa = 11.0 VDD392 pKa = 2.9 WHH394 pKa = 7.83 YY395 pKa = 11.76 GMTMQGDD402 pKa = 4.02 PTMGPSVLRR411 pKa = 11.84 AILPVITGTDD421 pKa = 3.22 CRR423 pKa = 11.84 DD424 pKa = 3.48 RR425 pKa = 11.84 LVGTDD430 pKa = 3.67 DD431 pKa = 4.25 AEE433 pKa = 5.08 LILGLGGRR441 pKa = 11.84 DD442 pKa = 3.81 RR443 pKa = 11.84 ITALAGDD450 pKa = 3.93 DD451 pKa = 4.45 RR452 pKa = 11.84 IFGGAGRR459 pKa = 11.84 DD460 pKa = 3.65 TIRR463 pKa = 11.84 AGDD466 pKa = 3.89 GDD468 pKa = 4.38 DD469 pKa = 5.52 FIDD472 pKa = 4.25 GGSGRR477 pKa = 11.84 DD478 pKa = 3.54 VIYY481 pKa = 10.17 TGAGADD487 pKa = 3.42 TVVISDD493 pKa = 4.01 LRR495 pKa = 11.84 GFDD498 pKa = 3.34 KK499 pKa = 11.13 LYY501 pKa = 11.04 DD502 pKa = 3.71 FDD504 pKa = 4.98 IGVDD508 pKa = 3.46 TLVIDD513 pKa = 4.46 IEE515 pKa = 5.06 GITPDD520 pKa = 4.97 DD521 pKa = 3.81 LSLNKK526 pKa = 8.57 WRR528 pKa = 11.84 LMASVDD534 pKa = 3.88 GCWTKK539 pKa = 10.79 LAFIPNAYY547 pKa = 9.99 RR548 pKa = 11.84 EE549 pKa = 4.37 GATLAEE555 pKa = 4.32 LLQPEE560 pKa = 4.7 VPGDD564 pKa = 3.47 ASTLYY569 pKa = 9.7 TLDD572 pKa = 2.96 VGSEE576 pKa = 3.98 VRR578 pKa = 11.84 GTLDD582 pKa = 3.62 FLEE585 pKa = 4.49 DD586 pKa = 3.79 RR587 pKa = 11.84 DD588 pKa = 3.86 WFGVEE593 pKa = 3.53 LRR595 pKa = 11.84 AGVTYY600 pKa = 10.73 RR601 pKa = 11.84 FDD603 pKa = 3.71 LVRR606 pKa = 11.84 DD607 pKa = 3.82 EE608 pKa = 5.37 ASSNPLPDD616 pKa = 4.07 PYY618 pKa = 10.82 FRR620 pKa = 11.84 LYY622 pKa = 10.67 DD623 pKa = 3.85 AEE625 pKa = 4.42 GQLLAANDD633 pKa = 4.78 DD634 pKa = 4.03 YY635 pKa = 12.13 SSLNSQLFYY644 pKa = 11.06 TPTEE648 pKa = 4.04 DD649 pKa = 4.01 ARR651 pKa = 11.84 VFAAAGAYY659 pKa = 10.01 ADD661 pKa = 4.36 AYY663 pKa = 9.63 EE664 pKa = 4.44 GGYY667 pKa = 9.97 VLSLAEE673 pKa = 4.44 AADD676 pKa = 4.09 FDD678 pKa = 4.79 AVPGDD683 pKa = 3.76 EE684 pKa = 4.38 STTAEE689 pKa = 4.19 IEE691 pKa = 4.37 VGGAAHH697 pKa = 6.93 GRR699 pKa = 11.84 IDD701 pKa = 4.76 YY702 pKa = 10.39 PGDD705 pKa = 4.03 DD706 pKa = 3.2 DD707 pKa = 3.79 WFRR710 pKa = 11.84 VEE712 pKa = 4.85 LRR714 pKa = 11.84 GGTTYY719 pKa = 10.95 RR720 pKa = 11.84 FYY722 pKa = 11.52 LSAEE726 pKa = 4.3 TGSDD730 pKa = 3.5 SPLWDD735 pKa = 4.03 PYY737 pKa = 10.97 FYY739 pKa = 10.75 LYY741 pKa = 10.64 DD742 pKa = 4.25 AEE744 pKa = 5.37 GEE746 pKa = 4.31 LIGANDD752 pKa = 3.82 DD753 pKa = 4.05 TGDD756 pKa = 4.16 SLNSSYY762 pKa = 11.15 QYY764 pKa = 10.86 TPDD767 pKa = 3.23 SDD769 pKa = 3.84 EE770 pKa = 4.92 LVFASARR777 pKa = 11.84 AFGDD781 pKa = 3.67 YY782 pKa = 9.42 GTGDD786 pKa = 3.66 YY787 pKa = 10.71 VLSVAEE793 pKa = 4.5 KK794 pKa = 9.57 EE795 pKa = 4.08 LGLFGMLQDD804 pKa = 3.54 VPAYY808 pKa = 9.75 NWYY811 pKa = 9.74 HH812 pKa = 5.85 GCSPTTGGSIIGYY825 pKa = 8.56 WDD827 pKa = 3.75 LNGYY831 pKa = 8.92 PDD833 pKa = 5.23 LFDD836 pKa = 4.81 AEE838 pKa = 4.4 GWEE841 pKa = 4.46 QISTTPSVQDD851 pKa = 3.69 QISSPAHH858 pKa = 4.97 NAKK861 pKa = 10.24 YY862 pKa = 10.59 DD863 pKa = 3.8 PTPDD867 pKa = 3.67 NPTLPVPPYY876 pKa = 10.11 TSIADD881 pKa = 3.67 FMGTSVDD888 pKa = 3.49 PLGYY892 pKa = 10.41 GSTYY896 pKa = 10.57 VSNFDD901 pKa = 3.08 GAYY904 pKa = 9.62 RR905 pKa = 11.84 GYY907 pKa = 9.9 PALTGSAFSSHH918 pKa = 5.63 TLYY921 pKa = 11.16 NSDD924 pKa = 3.13 TGEE927 pKa = 4.3 PGGSLWEE934 pKa = 4.25 EE935 pKa = 4.03 YY936 pKa = 10.34 VAEE939 pKa = 4.34 IDD941 pKa = 3.25 AGRR944 pKa = 11.84 PVAMTVDD951 pKa = 3.87 SNGDD955 pKa = 3.67 GITDD959 pKa = 3.52 HH960 pKa = 7.07 SIPGIGYY967 pKa = 8.19 EE968 pKa = 4.56 DD969 pKa = 4.04 RR970 pKa = 11.84 GADD973 pKa = 3.91 GVWYY977 pKa = 9.68 AAYY980 pKa = 6.51 TTWSEE985 pKa = 4.22 SEE987 pKa = 4.08 TPIWRR992 pKa = 11.84 EE993 pKa = 3.7 FRR995 pKa = 11.84 PTAPGAAWAVSYY1007 pKa = 8.71 LTFVLPEE1014 pKa = 4.09 GAEE1017 pKa = 3.92 AAPTGDD1023 pKa = 4.4 LALAMAGGAEE1033 pKa = 4.84 PITSEE1038 pKa = 3.91 PLTFTEE1044 pKa = 4.49 EE1045 pKa = 3.76 LHH1047 pKa = 6.63 EE1048 pKa = 4.2 IAVALEE1054 pKa = 3.69 IDD1056 pKa = 4.14 GEE1058 pKa = 4.27 LGAAEE1063 pKa = 4.73 EE1064 pKa = 4.75 KK1065 pKa = 10.66 ILMQAKK1071 pKa = 10.27 LIDD1074 pKa = 3.7 FAQPFDD1080 pKa = 4.03 DD1081 pKa = 4.87 LFVVV1085 pKa = 4.13
Molecular weight: 117.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.579
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.923
Patrickios 1.202
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A1B1PN51|A0A1B1PN51_9RHOB Uncharacterized protein OS=Yangia sp. CCB-MM3 OX=1792508 GN=AYJ57_06330 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.31 AGRR28 pKa = 11.84 AILNARR34 pKa = 11.84 RR35 pKa = 11.84 GRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.06 KK41 pKa = 10.57 LSAA44 pKa = 4.03
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4896
0
4896
1570501
30
5754
320.8
34.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.588 ± 0.043
0.901 ± 0.012
5.742 ± 0.04
6.359 ± 0.037
3.67 ± 0.024
8.914 ± 0.034
1.991 ± 0.02
4.881 ± 0.027
2.941 ± 0.029
10.479 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.768 ± 0.02
2.333 ± 0.019
5.237 ± 0.025
3.182 ± 0.019
6.657 ± 0.046
5.303 ± 0.031
5.307 ± 0.031
7.142 ± 0.032
1.388 ± 0.014
2.215 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here