Alistipes sp. An31A
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2054 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y4ARL2|A0A1Y4ARL2_9BACT Uncharacterized protein OS=Alistipes sp. An31A OX=1965631 GN=B5F90_05205 PE=4 SV=1
MM1 pKa = 7.04 KK2 pKa = 10.22 QLYY5 pKa = 9.75 LLPLLAILALTGCKK19 pKa = 9.65 KK20 pKa = 10.72 DD21 pKa = 4.46 DD22 pKa = 4.17 DD23 pKa = 4.46 ASRR26 pKa = 11.84 PGNLTIEE33 pKa = 4.27 KK34 pKa = 9.64 TEE36 pKa = 3.95 YY37 pKa = 10.48 TLDD40 pKa = 3.64 ADD42 pKa = 4.39 EE43 pKa = 4.95 EE44 pKa = 4.43 RR45 pKa = 11.84 TATVAFTAAADD56 pKa = 3.5 WTLSVVYY63 pKa = 10.53 DD64 pKa = 4.19 DD65 pKa = 5.58 AEE67 pKa = 4.51 SSADD71 pKa = 3.35 WLTVTPTSGTAGEE84 pKa = 4.26 QTVEE88 pKa = 4.36 LSATRR93 pKa = 11.84 NFRR96 pKa = 11.84 ASARR100 pKa = 11.84 TAYY103 pKa = 10.57 ADD105 pKa = 4.14 LACGEE110 pKa = 4.08 QSVRR114 pKa = 11.84 LTVTQTAASGDD125 pKa = 3.68 TDD127 pKa = 3.89 FTAQFDD133 pKa = 4.01 PDD135 pKa = 3.78 FAKK138 pKa = 10.45 EE139 pKa = 3.93 LQKK142 pKa = 10.92 QGIIADD148 pKa = 4.07 AGNITPEE155 pKa = 4.1 DD156 pKa = 3.79 MEE158 pKa = 5.42 KK159 pKa = 10.2 IAAITSLDD167 pKa = 3.48 VSGTEE172 pKa = 4.25 DD173 pKa = 3.53 APGALTSLQGIEE185 pKa = 4.16 YY186 pKa = 10.2 FEE188 pKa = 4.72 SLTEE192 pKa = 3.94 LEE194 pKa = 4.26 CRR196 pKa = 11.84 YY197 pKa = 10.14 NQLTSLDD204 pKa = 3.66 VSRR207 pKa = 11.84 NTALMEE213 pKa = 4.85 LEE215 pKa = 4.16 CQYY218 pKa = 11.47 NQLPEE223 pKa = 4.97 LDD225 pKa = 3.55 VSANTKK231 pKa = 9.87 LWLLICSSNSMEE243 pKa = 4.19 TLNVGANTALTYY255 pKa = 9.61 LYY257 pKa = 10.73 CNYY260 pKa = 10.57 NEE262 pKa = 4.35 LTTLDD267 pKa = 3.4 VSRR270 pKa = 11.84 NTEE273 pKa = 4.13 LMYY276 pKa = 10.64 LSCFNNKK283 pKa = 8.01 LTEE286 pKa = 4.23 LDD288 pKa = 3.28 VRR290 pKa = 11.84 ANTKK294 pKa = 10.21 LADD297 pKa = 4.28 LSCSNNSLTEE307 pKa = 3.76 LDD309 pKa = 3.43 VRR311 pKa = 11.84 ANTEE315 pKa = 4.35 LEE317 pKa = 4.29 MFDD320 pKa = 4.58 CSNNSLTEE328 pKa = 3.96 LDD330 pKa = 3.59 VSANTKK336 pKa = 9.72 LAWHH340 pKa = 6.96 FYY342 pKa = 10.66 CYY344 pKa = 10.79 GNQLTEE350 pKa = 4.55 LDD352 pKa = 4.05 VSNCTALEE360 pKa = 3.91 VLFCHH365 pKa = 7.07 DD366 pKa = 4.66 NPLTSLDD373 pKa = 3.62 LSRR376 pKa = 11.84 NTALTEE382 pKa = 4.1 LQCSNNQLTEE392 pKa = 4.11 LDD394 pKa = 3.75 LSRR397 pKa = 11.84 NTALTKK403 pKa = 10.33 LRR405 pKa = 11.84 CFDD408 pKa = 4.21 NEE410 pKa = 4.31 LTSLDD415 pKa = 3.61 LSRR418 pKa = 11.84 NTVLTDD424 pKa = 3.69 LYY426 pKa = 10.91 CGNNQLTSLDD436 pKa = 3.65 VSQNTALTEE445 pKa = 3.89 LSCFSNEE452 pKa = 4.52 LPSLDD457 pKa = 4.02 LSRR460 pKa = 11.84 NTALTLLDD468 pKa = 3.8 CSYY471 pKa = 11.51 NPGDD475 pKa = 3.99 GVSSFPVTAWFDD487 pKa = 3.81 NEE489 pKa = 4.38 TVPAGLDD496 pKa = 3.59 VYY498 pKa = 11.22 SSSWWYY504 pKa = 10.12 DD505 pKa = 3.27 DD506 pKa = 4.25 KK507 pKa = 11.82 NITIDD512 pKa = 3.83 FRR514 pKa = 11.84 KK515 pKa = 8.68 TEE517 pKa = 3.83
Molecular weight: 57.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.961
IPC_protein 3.973
Toseland 3.77
ProMoST 4.101
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.846
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.897
Nozaki 4.05
DTASelect 4.266
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.088
Patrickios 1.214
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.978
Protein with the highest isoelectric point:
>tr|A0A1Y4AE02|A0A1Y4AE02_9BACT Mannosyltransferase OS=Alistipes sp. An31A OX=1965631 GN=B5F90_06675 PE=4 SV=1
MM1 pKa = 7.49 PSSRR5 pKa = 11.84 RR6 pKa = 11.84 GPCGCRR12 pKa = 11.84 ASAAPRR18 pKa = 11.84 GGRR21 pKa = 11.84 RR22 pKa = 11.84 PPAPGRR28 pKa = 11.84 RR29 pKa = 11.84 SRR31 pKa = 11.84 RR32 pKa = 11.84 AAGGSPPRR40 pKa = 11.84 GAGRR44 pKa = 11.84 RR45 pKa = 11.84 GGVRR49 pKa = 11.84 PPGAGSAPRR58 pKa = 11.84 RR59 pKa = 11.84 PRR61 pKa = 11.84 CRR63 pKa = 11.84 PRR65 pKa = 4.6
Molecular weight: 6.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.369
IPC2_protein 10.804
IPC_protein 12.413
Toseland 12.574
ProMoST 13.071
Dawson 12.574
Bjellqvist 12.574
Wikipedia 13.056
Rodwell 12.076
Grimsley 12.618
Solomon 13.071
Lehninger 12.969
Nozaki 12.574
DTASelect 12.574
Thurlkill 12.574
EMBOSS 13.071
Sillero 12.574
Patrickios 11.798
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.181
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2054
0
2054
728213
38
1533
354.5
39.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.492 ± 0.063
1.305 ± 0.021
5.419 ± 0.036
7.055 ± 0.052
4.122 ± 0.032
7.51 ± 0.044
1.906 ± 0.021
5.406 ± 0.046
4.063 ± 0.053
9.743 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.544 ± 0.025
3.542 ± 0.037
4.223 ± 0.033
3.12 ± 0.029
7.157 ± 0.061
5.743 ± 0.039
5.617 ± 0.046
6.981 ± 0.044
1.223 ± 0.021
3.829 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here