Oribacterium sp. KHPX15
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3437 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H4D0W6|A0A1H4D0W6_9FIRM Predicted oxidoreductase of the aldo/keto reductase family OS=Oribacterium sp. KHPX15 OX=1855342 GN=SAMN05216349_12136 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 KK3 pKa = 9.52 IMLTISAVLLLSFLTACDD21 pKa = 3.56 MASGISTVEE30 pKa = 3.87 NSVSTTEE37 pKa = 3.88 NSINSAEE44 pKa = 4.21 NKK46 pKa = 10.0 QEE48 pKa = 4.26 DD49 pKa = 3.79 GGGIEE54 pKa = 4.26 GQDD57 pKa = 2.67 WRR59 pKa = 11.84 TWGTIDD65 pKa = 4.54 GYY67 pKa = 12.0 GNLSLDD73 pKa = 3.64 GQDD76 pKa = 4.21 IDD78 pKa = 4.04 VCACVFGDD86 pKa = 4.49 RR87 pKa = 11.84 VEE89 pKa = 4.83 LYY91 pKa = 10.84 YY92 pKa = 10.67 DD93 pKa = 3.59 EE94 pKa = 5.63 PKK96 pKa = 10.77 QNLFLQVDD104 pKa = 4.25 YY105 pKa = 11.04 PEE107 pKa = 5.0 KK108 pKa = 10.52 LTDD111 pKa = 3.53 EE112 pKa = 4.53 QYY114 pKa = 11.55 EE115 pKa = 4.27 KK116 pKa = 10.84 ATVEE120 pKa = 3.72 FDD122 pKa = 5.11 DD123 pKa = 5.28 YY124 pKa = 11.92 NDD126 pKa = 4.85 DD127 pKa = 3.48 GNTDD131 pKa = 2.83 VRR133 pKa = 11.84 IIVGEE138 pKa = 3.93 EE139 pKa = 3.88 DD140 pKa = 4.57 SPDD143 pKa = 3.36 MWWTFVWDD151 pKa = 3.82 KK152 pKa = 11.84 DD153 pKa = 3.73 DD154 pKa = 3.97 FVYY157 pKa = 9.96 MAALAYY163 pKa = 9.33 PPTEE167 pKa = 4.15 VEE169 pKa = 4.22 HH170 pKa = 6.77
Molecular weight: 19.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.834
Patrickios 1.85
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A1H4ECX6|A0A1H4ECX6_9FIRM SpaA domain-containing protein OS=Oribacterium sp. KHPX15 OX=1855342 GN=SAMN05216349_1332 PE=4 SV=1
MM1 pKa = 7.59 HH2 pKa = 7.51 KK3 pKa = 10.87 GKK5 pKa = 8.17 MTLQPKK11 pKa = 7.84 TRR13 pKa = 11.84 QRR15 pKa = 11.84 AKK17 pKa = 9.26 VHH19 pKa = 5.55 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 MATAGGRR31 pKa = 11.84 KK32 pKa = 8.88 VLAARR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 10.1 GRR42 pKa = 11.84 AALTVV47 pKa = 3.28
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.618
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.34
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3437
0
3437
1147060
28
4457
333.7
37.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.16 ± 0.05
1.286 ± 0.016
6.562 ± 0.039
7.478 ± 0.046
4.208 ± 0.029
6.936 ± 0.039
1.715 ± 0.019
7.505 ± 0.044
7.037 ± 0.036
8.389 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.019 ± 0.025
4.839 ± 0.034
3.272 ± 0.025
2.742 ± 0.022
4.104 ± 0.031
6.38 ± 0.038
5.576 ± 0.036
6.603 ± 0.034
0.89 ± 0.015
4.298 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here