Amino acid dipepetide frequency for Oribacterium sp. KHPX15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.9AlaAla: 6.9 ± 0.132	0.952AlaCys: 0.952 ± 0.032	4.556AlaAsp: 4.556 ± 0.076	5.674AlaGlu: 5.674 ± 0.094	3.084AlaPhe: 3.084 ± 0.06	5.456AlaGly: 5.456 ± 0.081	1.057AlaHis: 1.057 ± 0.032	4.971AlaIle: 4.971 ± 0.069	4.623AlaLys: 4.623 ± 0.073	6.483AlaLeu: 6.483 ± 0.088	2.297AlaMet: 2.297 ± 0.045	2.751AlaAsn: 2.751 ± 0.053	2.019AlaPro: 2.019 ± 0.053	1.911AlaGln: 1.911 ± 0.041	2.477AlaArg: 2.477 ± 0.049	4.3AlaSer: 4.3 ± 0.069	3.328AlaThr: 3.328 ± 0.063	5.446AlaVal: 5.446 ± 0.091	0.623AlaTrp: 0.623 ± 0.027	2.689AlaTyr: 2.689 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.816CysAla: 0.816 ± 0.031	0.219CysCys: 0.219 ± 0.014	0.887CysAsp: 0.887 ± 0.028	0.849CysGlu: 0.849 ± 0.028	0.609CysPhe: 0.609 ± 0.026	1.252CysGly: 1.252 ± 0.039	0.257CysHis: 0.257 ± 0.015	0.987CysIle: 0.987 ± 0.033	0.716CysLys: 0.716 ± 0.028	1.049CysLeu: 1.049 ± 0.033	0.357CysMet: 0.357 ± 0.018	0.566CysAsn: 0.566 ± 0.027	0.55CysPro: 0.55 ± 0.025	0.276CysGln: 0.276 ± 0.015	0.512CysArg: 0.512 ± 0.022	0.838CysSer: 0.838 ± 0.028	0.69CysThr: 0.69 ± 0.026	0.81CysVal: 0.81 ± 0.028	0.096CysTrp: 0.096 ± 0.008	0.522CysTyr: 0.522 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.486AspAla: 4.486 ± 0.07	0.761AspCys: 0.761 ± 0.028	4.143AspAsp: 4.143 ± 0.08	5.444AspGlu: 5.444 ± 0.074	3.139AspPhe: 3.139 ± 0.058	4.983AspGly: 4.983 ± 0.084	1.167AspHis: 1.167 ± 0.028	5.321AspIle: 5.321 ± 0.07	4.474AspLys: 4.474 ± 0.07	5.013AspLeu: 5.013 ± 0.079	2.039AspMet: 2.039 ± 0.044	2.943AspAsn: 2.943 ± 0.052	2.347AspPro: 2.347 ± 0.042	1.521AspGln: 1.521 ± 0.039	2.645AspArg: 2.645 ± 0.057	4.002AspSer: 4.002 ± 0.075	3.377AspThr: 3.377 ± 0.062	3.983AspVal: 3.983 ± 0.066	0.643AspTrp: 0.643 ± 0.026	3.188AspTyr: 3.188 ± 0.06	0.0AspXaa: 0.0 ± 0.0
Glu	5.688GluAla: 5.688 ± 0.095	0.747GluCys: 0.747 ± 0.026	4.882GluAsp: 4.882 ± 0.084	6.416GluGlu: 6.416 ± 0.097	2.573GluPhe: 2.573 ± 0.052	4.313GluGly: 4.313 ± 0.058	1.312GluHis: 1.312 ± 0.036	5.584GluIle: 5.584 ± 0.073	6.551GluLys: 6.551 ± 0.093	6.49GluLeu: 6.49 ± 0.085	2.223GluMet: 2.223 ± 0.044	4.25GluAsn: 4.25 ± 0.061	1.835GluPro: 1.835 ± 0.044	2.295GluGln: 2.295 ± 0.047	3.068GluArg: 3.068 ± 0.057	4.01GluSer: 4.01 ± 0.066	4.43GluThr: 4.43 ± 0.075	4.226GluVal: 4.226 ± 0.072	0.68GluTrp: 0.68 ± 0.023	3.208GluTyr: 3.208 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	2.785PheAla: 2.785 ± 0.057	0.706PheCys: 0.706 ± 0.025	3.003PheAsp: 3.003 ± 0.055	2.707PheGlu: 2.707 ± 0.049	1.874PhePhe: 1.874 ± 0.052	3.11PheGly: 3.11 ± 0.052	0.724PheHis: 0.724 ± 0.026	3.22PheIle: 3.22 ± 0.065	2.472PheLys: 2.472 ± 0.043	3.617PheLeu: 3.617 ± 0.068	1.239PheMet: 1.239 ± 0.034	1.958PheAsn: 1.958 ± 0.043	1.337PhePro: 1.337 ± 0.036	1.042PheGln: 1.042 ± 0.031	1.823PheArg: 1.823 ± 0.035	3.032PheSer: 3.032 ± 0.063	2.423PheThr: 2.423 ± 0.047	2.819PheVal: 2.819 ± 0.049	0.382PheTrp: 0.382 ± 0.019	1.803PheTyr: 1.803 ± 0.044	0.001PheXaa: 0.001 ± 0.001
Gly	4.846GlyAla: 4.846 ± 0.086	1.045GlyCys: 1.045 ± 0.03	3.987GlyAsp: 3.987 ± 0.06	4.297GlyGlu: 4.297 ± 0.066	3.281GlyPhe: 3.281 ± 0.05	4.653GlyGly: 4.653 ± 0.096	1.258GlyHis: 1.258 ± 0.038	5.93GlyIle: 5.93 ± 0.082	5.235GlyLys: 5.235 ± 0.069	5.475GlyLeu: 5.475 ± 0.078	2.262GlyMet: 2.262 ± 0.044	3.458GlyAsn: 3.458 ± 0.063	1.342GlyPro: 1.342 ± 0.038	1.906GlyGln: 1.906 ± 0.052	2.818GlyArg: 2.818 ± 0.058	4.619GlySer: 4.619 ± 0.073	4.242GlyThr: 4.242 ± 0.07	4.666GlyVal: 4.666 ± 0.082	0.728GlyTrp: 0.728 ± 0.03	3.317GlyTyr: 3.317 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.041HisAla: 1.041 ± 0.027	0.241HisCys: 0.241 ± 0.013	1.101HisAsp: 1.101 ± 0.033	1.168HisGlu: 1.168 ± 0.035	0.83HisPhe: 0.83 ± 0.028	1.207HisGly: 1.207 ± 0.034	0.409HisHis: 0.409 ± 0.034	1.418HisIle: 1.418 ± 0.039	1.092HisLys: 1.092 ± 0.032	1.34HisLeu: 1.34 ± 0.037	0.519HisMet: 0.519 ± 0.021	0.766HisAsn: 0.766 ± 0.028	0.842HisPro: 0.842 ± 0.032	0.473HisGln: 0.473 ± 0.02	0.696HisArg: 0.696 ± 0.026	1.04HisSer: 1.04 ± 0.029	0.923HisThr: 0.923 ± 0.027	1.096HisVal: 1.096 ± 0.033	0.141HisTrp: 0.141 ± 0.015	0.81HisTyr: 0.81 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.43IleAla: 5.43 ± 0.08	1.208IleCys: 1.208 ± 0.034	4.935IleAsp: 4.935 ± 0.07	4.935IleGlu: 4.935 ± 0.063	2.968IlePhe: 2.968 ± 0.06	4.869IleGly: 4.869 ± 0.083	1.336IleHis: 1.336 ± 0.035	5.491IleIle: 5.491 ± 0.088	4.919IleLys: 4.919 ± 0.066	6.471IleLeu: 6.471 ± 0.091	2.047IleMet: 2.047 ± 0.037	3.733IleAsn: 3.733 ± 0.066	3.145IlePro: 3.145 ± 0.058	1.948IleGln: 1.948 ± 0.048	3.467IleArg: 3.467 ± 0.062	5.411IleSer: 5.411 ± 0.085	4.457IleThr: 4.457 ± 0.06	4.758IleVal: 4.758 ± 0.07	0.568IleTrp: 0.568 ± 0.026	2.954IleTyr: 2.954 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	5.316LysAla: 5.316 ± 0.081	0.692LysCys: 0.692 ± 0.028	5.008LysAsp: 5.008 ± 0.072	6.126LysGlu: 6.126 ± 0.087	2.008LysPhe: 2.008 ± 0.041	4.127LysGly: 4.127 ± 0.057	1.19LysHis: 1.19 ± 0.033	4.73LysIle: 4.73 ± 0.066	6.053LysLys: 6.053 ± 0.08	5.809LysLeu: 5.809 ± 0.077	1.976LysMet: 1.976 ± 0.043	3.977LysAsn: 3.977 ± 0.058	2.148LysPro: 2.148 ± 0.048	2.008LysGln: 2.008 ± 0.043	2.894LysArg: 2.894 ± 0.06	3.975LysSer: 3.975 ± 0.065	4.04LysThr: 4.04 ± 0.06	4.323LysVal: 4.323 ± 0.063	0.656LysTrp: 0.656 ± 0.021	3.306LysTyr: 3.306 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	5.671LeuAla: 5.671 ± 0.068	1.192LeuCys: 1.192 ± 0.035	5.177LeuAsp: 5.177 ± 0.083	5.381LeuGlu: 5.381 ± 0.072	3.65LeuPhe: 3.65 ± 0.072	5.395LeuGly: 5.395 ± 0.074	1.404LeuHis: 1.404 ± 0.037	6.13LeuIle: 6.13 ± 0.085	6.379LeuLys: 6.379 ± 0.081	7.048LeuLeu: 7.048 ± 0.093	2.663LeuMet: 2.663 ± 0.048	4.131LeuAsn: 4.131 ± 0.069	3.196LeuPro: 3.196 ± 0.054	2.359LeuGln: 2.359 ± 0.048	3.746LeuArg: 3.746 ± 0.065	6.477LeuSer: 6.477 ± 0.088	4.861LeuThr: 4.861 ± 0.056	5.097LeuVal: 5.097 ± 0.068	0.678LeuTrp: 0.678 ± 0.027	3.256LeuTyr: 3.256 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	2.283MetAla: 2.283 ± 0.044	0.319MetCys: 0.319 ± 0.019	1.909MetAsp: 1.909 ± 0.042	2.126MetGlu: 2.126 ± 0.044	1.159MetPhe: 1.159 ± 0.034	2.001MetGly: 2.001 ± 0.042	0.48MetHis: 0.48 ± 0.02	2.255MetIle: 2.255 ± 0.055	2.344MetLys: 2.344 ± 0.046	2.592MetLeu: 2.592 ± 0.056	0.983MetMet: 0.983 ± 0.033	1.595MetAsn: 1.595 ± 0.035	1.156MetPro: 1.156 ± 0.033	0.931MetGln: 0.931 ± 0.027	1.179MetArg: 1.179 ± 0.033	1.984MetSer: 1.984 ± 0.042	1.826MetThr: 1.826 ± 0.039	1.897MetVal: 1.897 ± 0.042	0.187MetTrp: 0.187 ± 0.012	0.983MetTyr: 0.983 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.532AsnAla: 3.532 ± 0.058	0.65AsnCys: 0.65 ± 0.023	2.963AsnAsp: 2.963 ± 0.058	3.373AsnGlu: 3.373 ± 0.055	1.701AsnPhe: 1.701 ± 0.043	3.876AsnGly: 3.876 ± 0.07	0.894AsnHis: 0.894 ± 0.03	3.899AsnIle: 3.899 ± 0.058	3.009AsnLys: 3.009 ± 0.052	3.873AsnLeu: 3.873 ± 0.064	1.418AsnMet: 1.418 ± 0.037	2.321AsnAsn: 2.321 ± 0.051	2.085AsnPro: 2.085 ± 0.048	1.365AsnGln: 1.365 ± 0.036	2.144AsnArg: 2.144 ± 0.043	2.866AsnSer: 2.866 ± 0.054	2.594AsnThr: 2.594 ± 0.05	3.23AsnVal: 3.23 ± 0.055	0.447AsnTrp: 0.447 ± 0.019	2.149AsnTyr: 2.149 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	2.218ProAla: 2.218 ± 0.048	0.349ProCys: 0.349 ± 0.019	2.691ProAsp: 2.691 ± 0.049	3.888ProGlu: 3.888 ± 0.066	1.482ProPhe: 1.482 ± 0.037	2.381ProGly: 2.381 ± 0.051	0.51ProHis: 0.51 ± 0.024	2.023ProIle: 2.023 ± 0.041	1.981ProLys: 1.981 ± 0.054	2.506ProLeu: 2.506 ± 0.051	0.928ProMet: 0.928 ± 0.033	1.291ProAsn: 1.291 ± 0.033	0.614ProPro: 0.614 ± 0.024	0.833ProGln: 0.833 ± 0.026	0.958ProArg: 0.958 ± 0.03	1.826ProSer: 1.826 ± 0.041	1.577ProThr: 1.577 ± 0.042	2.911ProVal: 2.911 ± 0.065	0.288ProTrp: 0.288 ± 0.016	1.471ProTyr: 1.471 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	1.951GlnAla: 1.951 ± 0.045	0.303GlnCys: 0.303 ± 0.017	1.605GlnAsp: 1.605 ± 0.038	2.106GlnGlu: 2.106 ± 0.05	1.076GlnPhe: 1.076 ± 0.031	1.759GlnGly: 1.759 ± 0.044	0.449GlnHis: 0.449 ± 0.02	2.047GlnIle: 2.047 ± 0.044	2.261GlnLys: 2.261 ± 0.044	2.281GlnLeu: 2.281 ± 0.045	0.943GlnMet: 0.943 ± 0.027	1.588GlnAsn: 1.588 ± 0.048	0.792GlnPro: 0.792 ± 0.029	0.96GlnGln: 0.96 ± 0.044	1.187GlnArg: 1.187 ± 0.034	1.567GlnSer: 1.567 ± 0.038	1.388GlnThr: 1.388 ± 0.036	1.652GlnVal: 1.652 ± 0.038	0.266GlnTrp: 0.266 ± 0.014	1.245GlnTyr: 1.245 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.487ArgAla: 2.487 ± 0.048	0.479ArgCys: 0.479 ± 0.022	2.56ArgAsp: 2.56 ± 0.052	3.244ArgGlu: 3.244 ± 0.064	1.997ArgPhe: 1.997 ± 0.044	2.205ArgGly: 2.205 ± 0.052	0.732ArgHis: 0.732 ± 0.029	3.528ArgIle: 3.528 ± 0.059	3.414ArgLys: 3.414 ± 0.065	3.452ArgLeu: 3.452 ± 0.056	1.373ArgMet: 1.373 ± 0.035	2.177ArgAsn: 2.177 ± 0.047	1.18ArgPro: 1.18 ± 0.029	1.257ArgGln: 1.257 ± 0.035	1.896ArgArg: 1.896 ± 0.046	2.212ArgSer: 2.212 ± 0.047	2.133ArgThr: 2.133 ± 0.044	2.482ArgVal: 2.482 ± 0.051	0.377ArgTrp: 0.377 ± 0.019	1.85ArgTyr: 1.85 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.287SerAla: 4.287 ± 0.073	0.706SerCys: 0.706 ± 0.024	4.502SerAsp: 4.502 ± 0.071	4.925SerGlu: 4.925 ± 0.08	3.077SerPhe: 3.077 ± 0.053	5.367SerGly: 5.367 ± 0.082	1.076SerHis: 1.076 ± 0.032	4.58SerIle: 4.58 ± 0.071	3.914SerLys: 3.914 ± 0.062	5.28SerLeu: 5.28 ± 0.074	1.94SerMet: 1.94 ± 0.041	2.654SerAsn: 2.654 ± 0.054	1.795SerPro: 1.795 ± 0.037	1.766SerGln: 1.766 ± 0.042	2.589SerArg: 2.589 ± 0.055	4.406SerSer: 4.406 ± 0.093	3.229SerThr: 3.229 ± 0.06	4.491SerVal: 4.491 ± 0.069	0.576SerTrp: 0.576 ± 0.022	2.642SerTyr: 2.642 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.42ThrAla: 4.42 ± 0.077	0.595ThrCys: 0.595 ± 0.025	3.736ThrAsp: 3.736 ± 0.059	4.328ThrGlu: 4.328 ± 0.074	2.282ThrPhe: 2.282 ± 0.05	4.847ThrGly: 4.847 ± 0.084	0.822ThrHis: 0.822 ± 0.029	3.926ThrIle: 3.926 ± 0.059	3.101ThrLys: 3.101 ± 0.059	4.76ThrLeu: 4.76 ± 0.069	1.453ThrMet: 1.453 ± 0.035	2.37ThrAsn: 2.37 ± 0.049	2.171ThrPro: 2.171 ± 0.044	1.32ThrGln: 1.32 ± 0.034	1.93ThrArg: 1.93 ± 0.04	3.24ThrSer: 3.24 ± 0.051	3.263ThrThr: 3.263 ± 0.076	4.453ThrVal: 4.453 ± 0.079	0.498ThrTrp: 0.498 ± 0.021	2.243ThrTyr: 2.243 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	4.325ValAla: 4.325 ± 0.075	0.948ValCys: 0.948 ± 0.034	4.11ValAsp: 4.11 ± 0.062	4.24ValGlu: 4.24 ± 0.065	2.922ValPhe: 2.922 ± 0.053	3.976ValGly: 3.976 ± 0.077	1.041ValHis: 1.041 ± 0.033	5.246ValIle: 5.246 ± 0.075	4.455ValLys: 4.455 ± 0.073	5.953ValLeu: 5.953 ± 0.08	2.031ValMet: 2.031 ± 0.043	3.209ValAsn: 3.209 ± 0.058	2.472ValPro: 2.472 ± 0.049	1.671ValGln: 1.671 ± 0.043	2.667ValArg: 2.667 ± 0.056	4.844ValSer: 4.844 ± 0.064	4.167ValThr: 4.167 ± 0.069	4.464ValVal: 4.464 ± 0.067	0.552ValTrp: 0.552 ± 0.022	2.736ValTyr: 2.736 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.517TrpAla: 0.517 ± 0.023	0.137TrpCys: 0.137 ± 0.011	0.577TrpAsp: 0.577 ± 0.027	0.575TrpGlu: 0.575 ± 0.023	0.439TrpPhe: 0.439 ± 0.022	0.556TrpGly: 0.556 ± 0.025	0.204TrpHis: 0.204 ± 0.012	0.685TrpIle: 0.685 ± 0.024	0.717TrpLys: 0.717 ± 0.024	0.801TrpLeu: 0.801 ± 0.029	0.293TrpMet: 0.293 ± 0.015	0.54TrpAsn: 0.54 ± 0.024	0.201TrpPro: 0.201 ± 0.012	0.324TrpGln: 0.324 ± 0.02	0.331TrpArg: 0.331 ± 0.017	0.479TrpSer: 0.479 ± 0.024	0.444TrpThr: 0.444 ± 0.02	0.503TrpVal: 0.503 ± 0.022	0.128TrpTrp: 0.128 ± 0.009	0.449TrpTyr: 0.449 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.595TyrAla: 2.595 ± 0.049	0.609TyrCys: 0.609 ± 0.023	3.288TyrAsp: 3.288 ± 0.068	2.979TyrGlu: 2.979 ± 0.051	1.925TyrPhe: 1.925 ± 0.037	3.032TyrGly: 3.032 ± 0.063	0.829TyrHis: 0.829 ± 0.024	3.079TyrIle: 3.079 ± 0.054	2.656TyrLys: 2.656 ± 0.044	3.6TyrLeu: 3.6 ± 0.055	1.204TyrMet: 1.204 ± 0.033	2.11TyrAsn: 2.11 ± 0.046	1.496TyrPro: 1.496 ± 0.035	1.258TyrGln: 1.258 ± 0.033	2.011TyrArg: 2.011 ± 0.047	2.677TyrSer: 2.677 ± 0.058	2.366TyrThr: 2.366 ± 0.046	2.719TyrVal: 2.719 ± 0.055	0.386TyrTrp: 0.386 ± 0.02	2.165TyrTyr: 2.165 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.003

Statistics based on 3437 proteins (1147060 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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