Changjiang tombus-like virus 19
Average proteome isoelectric point is 8.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFZ9|A0A1L3KFZ9_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 19 OX=1922812 PE=4 SV=1
MM1 pKa = 7.48 VEE3 pKa = 4.0 QRR5 pKa = 11.84 PHH7 pKa = 5.87 ICYY10 pKa = 10.45 GNCFHH15 pKa = 7.21 NVVRR19 pKa = 11.84 SFNGRR24 pKa = 11.84 YY25 pKa = 8.03 FKK27 pKa = 10.53 PSPRR31 pKa = 11.84 PRR33 pKa = 11.84 RR34 pKa = 11.84 GDD36 pKa = 3.33 IDD38 pKa = 3.06 WGLVHH43 pKa = 7.25 KK44 pKa = 10.65 IVDD47 pKa = 4.02 DD48 pKa = 4.17 LSDD51 pKa = 4.95 RR52 pKa = 11.84 IIKK55 pKa = 10.02 NAICDD60 pKa = 3.46 NDD62 pKa = 4.13 GPNEE66 pKa = 4.14 SQKK69 pKa = 9.87 TGYY72 pKa = 10.19 LFNAGKK78 pKa = 10.31 GGVMNFINDD87 pKa = 3.42 APGKK91 pKa = 8.96 NKK93 pKa = 9.58 RR94 pKa = 11.84 RR95 pKa = 11.84 YY96 pKa = 9.56 RR97 pKa = 11.84 SALDD101 pKa = 3.29 QILAKK106 pKa = 10.71 GFTPLKK112 pKa = 8.69 HH113 pKa = 6.41 SRR115 pKa = 11.84 MSVFVKK121 pKa = 10.44 RR122 pKa = 11.84 EE123 pKa = 3.77 SFYY126 pKa = 11.4 KK127 pKa = 10.16 EE128 pKa = 3.52 GKK130 pKa = 9.87 APRR133 pKa = 11.84 LIMGRR138 pKa = 11.84 DD139 pKa = 3.3 PRR141 pKa = 11.84 FALFYY146 pKa = 9.43 NTVIEE151 pKa = 4.62 PFDD154 pKa = 3.67 KK155 pKa = 10.95 PFFNLPQCTYY165 pKa = 10.63 NDD167 pKa = 3.79 NYY169 pKa = 11.43 DD170 pKa = 3.79 SLGHH174 pKa = 6.72 KK175 pKa = 10.21 FGEE178 pKa = 4.9 LVGQWFCEE186 pKa = 3.92 NDD188 pKa = 3.3 FSQCEE193 pKa = 4.17 SSIQPFWLDD202 pKa = 3.3 LQLRR206 pKa = 11.84 CMNKK210 pKa = 8.82 VLTAIKK216 pKa = 9.93 QQANLKK222 pKa = 8.0 MAWAMKK228 pKa = 9.54 IVKK231 pKa = 9.78 DD232 pKa = 4.13 GQWRR236 pKa = 11.84 EE237 pKa = 3.84 YY238 pKa = 9.66 ITFKK242 pKa = 10.82 FQEE245 pKa = 4.57 CTGSGDD251 pKa = 4.11 RR252 pKa = 11.84 DD253 pKa = 3.52 TSSLNGFVNWVSSRR267 pKa = 11.84 YY268 pKa = 9.43 FLIKK272 pKa = 10.55 NIEE275 pKa = 4.24 PNCKK279 pKa = 9.83 VSSDD283 pKa = 3.91 CAGCSTDD290 pKa = 4.75 KK291 pKa = 10.99 FLLKK295 pKa = 10.71 GDD297 pKa = 4.3 DD298 pKa = 4.16 NVMRR302 pKa = 11.84 WPRR305 pKa = 11.84 GVTEE309 pKa = 4.88 FKK311 pKa = 9.88 NTYY314 pKa = 8.02 TLFGLDD320 pKa = 2.99 AKK322 pKa = 10.86 LIIRR326 pKa = 11.84 KK327 pKa = 7.43 TPEE330 pKa = 3.63 EE331 pKa = 4.12 VEE333 pKa = 4.33 FCSGHH338 pKa = 6.43 FIEE341 pKa = 4.96 TTPGNYY347 pKa = 10.1 LYY349 pKa = 10.58 VQKK352 pKa = 10.45 LVKK355 pKa = 10.6 LIDD358 pKa = 3.48 SLQRR362 pKa = 11.84 CLNSDD367 pKa = 3.35 ALKK370 pKa = 10.55 RR371 pKa = 11.84 GWVAQYY377 pKa = 10.47 YY378 pKa = 10.14 KK379 pKa = 10.87 SLGLMYY385 pKa = 10.4 KK386 pKa = 9.94 VLYY389 pKa = 10.17 RR390 pKa = 11.84 DD391 pKa = 3.07 IPVYY395 pKa = 10.7 SEE397 pKa = 3.55 IAEE400 pKa = 4.18 FLLNTNFDD408 pKa = 3.58 GGINANLINSWNLTNSFKK426 pKa = 11.02 QSDD429 pKa = 4.08 GCVRR433 pKa = 11.84 RR434 pKa = 11.84 VDD436 pKa = 3.51 QSLCEE441 pKa = 4.25 LSVAMANGLSIPEE454 pKa = 4.01 VEE456 pKa = 5.3 RR457 pKa = 11.84 IKK459 pKa = 11.16 DD460 pKa = 3.52 WFSTNALHH468 pKa = 7.16 FPASMTKK475 pKa = 9.69 RR476 pKa = 11.84 CNLRR480 pKa = 11.84 ACKK483 pKa = 7.39 TTPKK487 pKa = 8.96 VTEE490 pKa = 4.0 VEE492 pKa = 3.98 QEE494 pKa = 3.86 IFNSCFANAWDD505 pKa = 4.35 TLTKK509 pKa = 10.35 DD510 pKa = 3.64 KK511 pKa = 11.05 RR512 pKa = 11.84 FAKK515 pKa = 10.24 IKK517 pKa = 9.4 QRR519 pKa = 11.84 YY520 pKa = 7.8 NRR522 pKa = 11.84 VLKK525 pKa = 10.81 GG526 pKa = 3.24
Molecular weight: 60.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.38
IPC2_protein 8.317
IPC_protein 8.141
Toseland 8.346
ProMoST 8.741
Dawson 9.033
Bjellqvist 9.151
Wikipedia 9.077
Rodwell 9.151
Grimsley 8.448
Solomon 9.092
Lehninger 9.077
Nozaki 9.355
DTASelect 8.946
Thurlkill 9.048
EMBOSS 9.209
Sillero 9.297
Patrickios 4.533
IPC_peptide 9.077
IPC2_peptide 7.995
IPC2.peptide.svr19 7.884
Protein with the highest isoelectric point:
>tr|A0A1L3KFZ9|A0A1L3KFZ9_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 19 OX=1922812 PE=4 SV=1
MM1 pKa = 7.87 SDD3 pKa = 3.65 QNVQTPRR10 pKa = 11.84 NRR12 pKa = 11.84 GGRR15 pKa = 11.84 QRR17 pKa = 11.84 PAQQNRR23 pKa = 11.84 QHH25 pKa = 6.23 LQQVVNTQHH34 pKa = 7.64 RR35 pKa = 11.84 IYY37 pKa = 10.64 QFTKK41 pKa = 10.14 VYY43 pKa = 9.13 TAALDD48 pKa = 3.35 KK49 pKa = 11.16 GFKK52 pKa = 10.17 DD53 pKa = 3.35 RR54 pKa = 11.84 AALNTFTTWVNANIASIDD72 pKa = 3.59 PASIRR77 pKa = 11.84 VCNEE81 pKa = 3.64 CGSSDD86 pKa = 6.16 LIPCDD91 pKa = 3.65 HH92 pKa = 7.27 FIVPAAPNAVVVNAHH107 pKa = 5.88 QYY109 pKa = 9.06 FAVPAGPTNFRR120 pKa = 11.84 RR121 pKa = 11.84 RR122 pKa = 11.84 FMWVGGIRR130 pKa = 11.84 RR131 pKa = 11.84 MFTWPSYY138 pKa = 10.58 DD139 pKa = 3.21 ASINNNHH146 pKa = 6.28 NLGGFHH152 pKa = 7.23 NSDD155 pKa = 3.48 IDD157 pKa = 3.97 DD158 pKa = 3.76 NMIIDD163 pKa = 3.69 EE164 pKa = 4.58 MYY166 pKa = 11.05 NYY168 pKa = 9.69 IQLNMCTSYY177 pKa = 11.08 LINGSVDD184 pKa = 2.7 RR185 pKa = 11.84 RR186 pKa = 11.84 ARR188 pKa = 11.84 LEE190 pKa = 4.2 HH191 pKa = 5.86 CHH193 pKa = 6.72 KK194 pKa = 10.68 LALKK198 pKa = 9.86 YY199 pKa = 10.64 CEE201 pKa = 4.12 EE202 pKa = 4.17 HH203 pKa = 6.78 SLRR206 pKa = 11.84 NPDD209 pKa = 3.37 PTTTHH214 pKa = 7.72 AIQNTIQRR222 pKa = 11.84 ACDD225 pKa = 3.29 NAEE228 pKa = 3.43 NDD230 pKa = 3.51 MLYY233 pKa = 10.84 EE234 pKa = 4.16 RR235 pKa = 11.84 TNPTHH240 pKa = 6.73 NLLNPYY246 pKa = 10.23 ALLDD250 pKa = 3.78 GVVNGVKK257 pKa = 10.38 YY258 pKa = 10.59 CLNATARR265 pKa = 11.84 GIVHH269 pKa = 6.11 MRR271 pKa = 11.84 HH272 pKa = 6.41 PIIAGVITVMVFRR285 pKa = 11.84 NARR288 pKa = 11.84 WLQSLTWRR296 pKa = 11.84 FLYY299 pKa = 10.71 APAGRR304 pKa = 11.84 MVINSALSAMEE315 pKa = 3.79 TTRR318 pKa = 11.84 QIMRR322 pKa = 11.84 GSAVHH327 pKa = 6.78 LNNLANWILNVSGAMRR343 pKa = 11.84 TSYY346 pKa = 10.82 CAGSVMQWLNNGRR359 pKa = 11.84 TFATEE364 pKa = 4.17 TVSTMSSGVSMDD376 pKa = 5.15 DD377 pKa = 2.63 ILSRR381 pKa = 11.84 VRR383 pKa = 11.84 ALDD386 pKa = 3.47 AVII389 pKa = 4.69
Molecular weight: 43.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.512
IPC2_protein 8.39
IPC_protein 8.361
Toseland 8.141
ProMoST 8.741
Dawson 8.902
Bjellqvist 9.18
Wikipedia 8.931
Rodwell 8.916
Grimsley 8.112
Solomon 9.033
Lehninger 9.004
Nozaki 9.282
DTASelect 8.946
Thurlkill 8.946
EMBOSS 9.063
Sillero 9.238
Patrickios 4.291
IPC_peptide 9.019
IPC2_peptide 8.595
IPC2.peptide.svr19 8.374
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
915
389
526
457.5
52.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.667 ± 1.594
2.951 ± 0.392
5.574 ± 0.425
3.607 ± 0.796
5.027 ± 1.196
5.792 ± 0.401
2.295 ± 0.803
5.683 ± 0.3
5.246 ± 2.281
7.65 ± 0.437
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.951 ± 0.716
8.197 ± 0.81
3.934 ± 0.048
3.934 ± 0.268
6.995 ± 0.6
6.23 ± 0.195
5.464 ± 0.751
6.339 ± 0.529
1.967 ± 0.103
3.497 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here