Delphinapterus leucas (Beluga whale)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40783 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Y9PN18|A0A2Y9PN18_DELLE Isoform of A0A2Y9PCP3 transmembrane and coiled-coil domain-containing protein 6 isoform X4 OS=Delphinapterus leucas OX=9749 GN=TMCO6 PE=4 SV=1
MM1 pKa = 7.3 EE2 pKa = 5.17 AVSLVLFVLGLLLLPLLAVLLMALCVRR29 pKa = 11.84 CRR31 pKa = 11.84 EE32 pKa = 4.25 LPGSYY37 pKa = 8.67 DD38 pKa = 3.24 TAASDD43 pKa = 3.91 GLTPSSIVIKK53 pKa = 9.83 PPPTVAPWPPATSYY67 pKa = 11.3 PPVTYY72 pKa = 10.68 LLQSQPDD79 pKa = 3.91 LLRR82 pKa = 11.84 IPRR85 pKa = 11.84 SPQPPGGSPRR95 pKa = 11.84 MPSSQQDD102 pKa = 3.01 SDD104 pKa = 3.88 GANSVASYY112 pKa = 10.42 EE113 pKa = 4.12 NEE115 pKa = 3.82 EE116 pKa = 4.38 AVCEE120 pKa = 4.31 DD121 pKa = 3.54 ADD123 pKa = 3.94 EE124 pKa = 6.05 DD125 pKa = 4.37 EE126 pKa = 5.92 DD127 pKa = 4.86 EE128 pKa = 4.15 EE129 pKa = 5.22 DD130 pKa = 3.81 YY131 pKa = 11.56 PEE133 pKa = 5.32 GYY135 pKa = 10.61 LVVLPDD141 pKa = 3.71 NVPATGAAVPPAPASSNPGLRR162 pKa = 11.84 DD163 pKa = 3.06 SAFSMEE169 pKa = 4.23 SGEE172 pKa = 4.99 DD173 pKa = 3.63 YY174 pKa = 11.66 VNVPEE179 pKa = 4.96 SEE181 pKa = 4.26 GSADD185 pKa = 3.47 ASLDD189 pKa = 3.39 GSRR192 pKa = 11.84 EE193 pKa = 3.99 YY194 pKa = 11.72 VNVSQEE200 pKa = 4.24 LPPVARR206 pKa = 11.84 TKK208 pKa = 10.27 PAGRR212 pKa = 11.84 SSQEE216 pKa = 3.88 VEE218 pKa = 4.13 GEE220 pKa = 3.96 EE221 pKa = 4.31 APDD224 pKa = 3.64 YY225 pKa = 11.32 EE226 pKa = 4.44 NLQIHH231 pKa = 6.6
Molecular weight: 24.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.63
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.668
EMBOSS 3.694
Sillero 3.935
Patrickios 1.863
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|A0A2Y9M5L6|A0A2Y9M5L6_DELLE Phosphodiesterase OS=Delphinapterus leucas OX=9749 GN=PDE4C PE=3 SV=1
MM1 pKa = 7.18 GWAALAGPARR11 pKa = 11.84 PRR13 pKa = 11.84 RR14 pKa = 11.84 VLQTARR20 pKa = 11.84 RR21 pKa = 11.84 ARR23 pKa = 11.84 QTTLSRR29 pKa = 11.84 PGPPDD34 pKa = 3.06 QTALAGPHH42 pKa = 3.99 QTRR45 pKa = 11.84 PRR47 pKa = 11.84 RR48 pKa = 11.84 VLQAALSRR56 pKa = 11.84 PGPPDD61 pKa = 2.82 QAARR65 pKa = 11.84 PRR67 pKa = 11.84 PVRR70 pKa = 11.84 PRR72 pKa = 11.84 SPGRR76 pKa = 11.84 ARR78 pKa = 11.84 QTRR81 pKa = 11.84 PRR83 pKa = 11.84 SPRR86 pKa = 11.84 PARR89 pKa = 11.84 PRR91 pKa = 11.84 RR92 pKa = 11.84 VLQTALSRR100 pKa = 11.84 PGPPDD105 pKa = 3.0 RR106 pKa = 11.84 AGSSRR111 pKa = 11.84 PRR113 pKa = 11.84 AGPARR118 pKa = 11.84 PRR120 pKa = 11.84 AGSSRR125 pKa = 11.84 PRR127 pKa = 11.84 AGPAGPRR134 pKa = 11.84 QAHH137 pKa = 3.87 QTARR141 pKa = 11.84 ARR143 pKa = 11.84 SSRR146 pKa = 11.84 PRR148 pKa = 11.84 SPGRR152 pKa = 11.84 VRR154 pKa = 11.84 QTRR157 pKa = 11.84 PRR159 pKa = 11.84 SLGPPDD165 pKa = 3.69 QPEE168 pKa = 4.02 LAGPARR174 pKa = 11.84 SRR176 pKa = 11.84 RR177 pKa = 11.84 VLRR180 pKa = 11.84 TAPGRR185 pKa = 11.84 ARR187 pKa = 11.84 QVLQTTVSRR196 pKa = 11.84 PGPPNQATRR205 pKa = 11.84 AGPTRR210 pKa = 11.84 RR211 pKa = 11.84 RR212 pKa = 11.84 SPGRR216 pKa = 11.84 VRR218 pKa = 11.84 QTRR221 pKa = 11.84 PSSPSQAAPGPSDD234 pKa = 3.35 PAPRR238 pKa = 11.84 ARR240 pKa = 11.84 QAALTRR246 pKa = 11.84 ARR248 pKa = 11.84 QAAPGHH254 pKa = 6.84 PDD256 pKa = 3.06 RR257 pKa = 11.84 ALQAGSARR265 pKa = 11.84 PGRR268 pKa = 11.84 ARR270 pKa = 11.84 PGPPDD275 pKa = 3.2 QTALARR281 pKa = 11.84 PQQTRR286 pKa = 11.84 PRR288 pKa = 11.84 RR289 pKa = 11.84 VLQAALSRR297 pKa = 11.84 PGPPDD302 pKa = 3.18 QATHH306 pKa = 7.16 PGPARR311 pKa = 11.84 PARR314 pKa = 11.84 PRR316 pKa = 11.84 PPGRR320 pKa = 11.84 APQTGPRR327 pKa = 11.84 SPAPARR333 pKa = 11.84 PNRR336 pKa = 11.84 TCRR339 pKa = 11.84 ARR341 pKa = 11.84 QAVAGPPDD349 pKa = 3.9 RR350 pKa = 11.84 GLQAGATRR358 pKa = 11.84 PRR360 pKa = 11.84 RR361 pKa = 11.84 VRR363 pKa = 11.84 QTTRR367 pKa = 11.84 WALQTALSRR376 pKa = 11.84 PGPPGRR382 pKa = 11.84 ARR384 pKa = 11.84 PGPPDD389 pKa = 3.2 QAALTRR395 pKa = 11.84 TRR397 pKa = 11.84 QTRR400 pKa = 11.84 PRR402 pKa = 11.84 ATGQAPGGPPDD413 pKa = 4.13 PARR416 pKa = 11.84 QGARR420 pKa = 11.84 QATLARR426 pKa = 11.84 TRR428 pKa = 11.84 QTRR431 pKa = 11.84 PRR433 pKa = 11.84 SAAQAAPGPPDD444 pKa = 3.64 PARR447 pKa = 11.84 QARR450 pKa = 11.84 PAALARR456 pKa = 11.84 ARR458 pKa = 11.84 QPRR461 pKa = 11.84 PRR463 pKa = 11.84 AASQAAPGPADD474 pKa = 3.79 PARR477 pKa = 11.84 QAALTRR483 pKa = 11.84 ARR485 pKa = 11.84 PTRR488 pKa = 11.84 PRR490 pKa = 11.84 SGPARR495 pKa = 11.84 PRR497 pKa = 11.84 LPPSPSAAEE506 pKa = 4.0 PSRR509 pKa = 11.84 ARR511 pKa = 11.84 NPASLRR517 pKa = 11.84 LPSLAIAPPPAASGPSSRR535 pKa = 11.84 PPGLLSTSLRR545 pKa = 11.84 ACQPGRR551 pKa = 11.84 QPSPCC556 pKa = 3.86
Molecular weight: 58.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.426
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.901
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18374
22409
40783
29353646
24
34744
719.8
80.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.125 ± 0.013
2.092 ± 0.01
4.879 ± 0.008
7.353 ± 0.015
3.412 ± 0.007
6.548 ± 0.016
2.58 ± 0.005
4.119 ± 0.009
5.725 ± 0.014
9.863 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.073 ± 0.005
3.482 ± 0.009
6.492 ± 0.02
4.961 ± 0.012
5.833 ± 0.011
8.577 ± 0.014
5.266 ± 0.009
5.959 ± 0.009
1.158 ± 0.004
2.493 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here