Candidatus Rickettsiella viridis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Rickettsiella

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5UWR4|A0A2Z5UWR4_9COXI 2 3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Candidatus Rickettsiella viridis OX=676208 GN=gpmI PE=3 SV=1
MM1 pKa = 7.74CLLCGWVYY9 pKa = 11.23DD10 pKa = 4.24EE11 pKa = 5.16EE12 pKa = 6.3KK13 pKa = 11.15GDD15 pKa = 4.04ADD17 pKa = 3.76EE18 pKa = 5.28GLAPGTRR25 pKa = 11.84WEE27 pKa = 5.4DD28 pKa = 3.23IPVTWRR34 pKa = 11.84CPEE37 pKa = 4.18CGAMKK42 pKa = 10.32EE43 pKa = 3.95DD44 pKa = 3.9FEE46 pKa = 4.55MVEE49 pKa = 4.16LL50 pKa = 4.48

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5V6X0|A0A2Z5V6X0_9COXI Phosphoglucosamine mutase OS=Candidatus Rickettsiella viridis OX=676208 GN=glmM PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.32QPSNLKK11 pKa = 10.19RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84LILKK32 pKa = 9.83RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.51GRR39 pKa = 11.84LRR41 pKa = 11.84LTAA44 pKa = 4.67

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1355

0

1355

440673

37

3882

325.2

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.136 ± 0.07

1.111 ± 0.022

4.665 ± 0.047

5.822 ± 0.072

4.377 ± 0.06

5.923 ± 0.083

2.354 ± 0.035

7.268 ± 0.052

6.569 ± 0.08

11.761 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.03

4.624 ± 0.074

4.131 ± 0.037

4.952 ± 0.066

4.556 ± 0.064

6.491 ± 0.049

5.37 ± 0.072

5.659 ± 0.064

1.073 ± 0.026

3.191 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski