Fowl aviadenovirus A
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286QWS6|A0A286QWS6_9ADEN ORF2 OS=Fowl aviadenovirus A OX=190061 PE=4 SV=1
MM1 pKa = 7.63 NDD3 pKa = 3.15 EE4 pKa = 4.75 QILEE8 pKa = 4.3 MVLQHH13 pKa = 5.07 QQRR16 pKa = 11.84 RR17 pKa = 11.84 QQEE20 pKa = 4.11 AEE22 pKa = 3.99 RR23 pKa = 11.84 EE24 pKa = 4.16 EE25 pKa = 4.36 EE26 pKa = 4.23 VGDD29 pKa = 5.04 DD30 pKa = 4.3 MEE32 pKa = 7.76 DD33 pKa = 4.78 DD34 pKa = 4.18 EE35 pKa = 7.8 DD36 pKa = 6.52 DD37 pKa = 6.24 DD38 pKa = 4.67 GLQMPTPLHH47 pKa = 7.17 AYY49 pKa = 9.65 QLLCYY54 pKa = 10.46 DD55 pKa = 3.51 SFEE58 pKa = 4.49 LHH60 pKa = 6.89 FGGCACHH67 pKa = 6.28 GLPLHH72 pKa = 6.94 RR73 pKa = 11.84 MGLSACHH80 pKa = 6.98 LAPSDD85 pKa = 3.25 LATYY89 pKa = 9.47 VWARR93 pKa = 11.84 LEE95 pKa = 4.64 DD96 pKa = 4.18 DD97 pKa = 4.62 LNVAGVYY104 pKa = 8.87 FVAMWASPGFSDD116 pKa = 4.83 FSPVFMQRR124 pKa = 11.84 PIGNVCGMLIHH135 pKa = 6.83 VDD137 pKa = 3.35 LHH139 pKa = 6.28 SRR141 pKa = 11.84 LPFLIAVSRR150 pKa = 11.84 LGEE153 pKa = 4.34 AGGSPCLYY161 pKa = 9.01 MRR163 pKa = 11.84 KK164 pKa = 8.94 IDD166 pKa = 4.28 VDD168 pKa = 4.36 LDD170 pKa = 3.56 TQRR173 pKa = 11.84 VHH175 pKa = 7.51 FYY177 pKa = 10.28 TEE179 pKa = 3.73 DD180 pKa = 3.29 WFSEE184 pKa = 4.33 FANLLYY190 pKa = 9.78 YY191 pKa = 9.76 WQMSEE196 pKa = 3.69 WKK198 pKa = 9.84 HH199 pKa = 5.12 LAEE202 pKa = 5.13 RR203 pKa = 11.84 MQQ205 pKa = 3.76
Molecular weight: 23.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.212
IPC2_protein 4.482
IPC_protein 4.444
Toseland 4.291
ProMoST 4.571
Dawson 4.418
Bjellqvist 4.558
Wikipedia 4.304
Rodwell 4.291
Grimsley 4.202
Solomon 4.406
Lehninger 4.368
Nozaki 4.52
DTASelect 4.711
Thurlkill 4.304
EMBOSS 4.329
Sillero 4.571
Patrickios 2.015
IPC_peptide 4.418
IPC2_peptide 4.558
IPC2.peptide.svr19 4.467
Protein with the highest isoelectric point:
>tr|A0A1L5YQN3|A0A1L5YQN3_9ADEN Isoform of A0A286QWU6 L5 fiber 2 OS=Fowl aviadenovirus A OX=190061 GN=L5 PE=4 SV=1
MM1 pKa = 7.74 SILISPSDD9 pKa = 3.59 NRR11 pKa = 11.84 GWGANMRR18 pKa = 11.84 YY19 pKa = 9.47 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ASMRR26 pKa = 11.84 GVGRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 LTLRR36 pKa = 11.84 QLLGLGSRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 SRR50 pKa = 11.84 PTTVSNRR57 pKa = 11.84 LVVVSTRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 SSRR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 3.37
Molecular weight: 8.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.487
IPC2_protein 11.184
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.062
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26
7
33
11317
72
1121
342.9
38.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.05 ± 0.442
1.732 ± 0.263
5.364 ± 0.321
5.337 ± 0.538
4.038 ± 0.258
6.53 ± 0.389
2.518 ± 0.278
4.074 ± 0.223
3.561 ± 0.243
9.119 ± 0.404
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.501 ± 0.187
4.489 ± 0.443
6.671 ± 0.268
4.091 ± 0.223
7.122 ± 0.623
6.777 ± 0.319
6.495 ± 0.508
6.539 ± 0.277
1.317 ± 0.123
3.676 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here