Human adenovirus sp.
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6HVP9|A0A1W6HVP9_9ADEN E3-19K OS=Human adenovirus sp. OX=1907210 PE=3 SV=1
MM1 pKa = 7.34 RR2 pKa = 11.84 HH3 pKa = 6.25 LRR5 pKa = 11.84 FLPQEE10 pKa = 4.33 IISAEE15 pKa = 4.03 TGNEE19 pKa = 3.27 ILEE22 pKa = 4.52 FVVHH26 pKa = 6.97 ALMGDD31 pKa = 3.71 DD32 pKa = 4.84 PEE34 pKa = 5.93 PPVQLFEE41 pKa = 4.7 PPTLQEE47 pKa = 4.5 LYY49 pKa = 10.31 DD50 pKa = 4.17 LEE52 pKa = 4.77 VEE54 pKa = 4.35 GSEE57 pKa = 4.86 DD58 pKa = 3.8 SNEE61 pKa = 3.83 EE62 pKa = 3.77 AVNGFFTDD70 pKa = 3.97 SMLLAANEE78 pKa = 4.13 GLEE81 pKa = 4.61 LDD83 pKa = 4.47 PPLDD87 pKa = 3.81 TFDD90 pKa = 3.65 TPGVIVEE97 pKa = 4.52 SGTGVRR103 pKa = 11.84 KK104 pKa = 9.9 LPDD107 pKa = 4.4 LGSVDD112 pKa = 4.89 CDD114 pKa = 3.2 LHH116 pKa = 8.32 CYY118 pKa = 10.19 EE119 pKa = 5.87 DD120 pKa = 4.7 GFPLSDD126 pKa = 3.31 EE127 pKa = 4.09 EE128 pKa = 5.17 DD129 pKa = 3.58 RR130 pKa = 11.84 EE131 pKa = 4.4 KK132 pKa = 11.14 EE133 pKa = 4.08 QFMQTAAGEE142 pKa = 4.52 GVKK145 pKa = 10.16 AASVGFQLDD154 pKa = 3.96 CPEE157 pKa = 4.73 LPGHH161 pKa = 5.76 GCKK164 pKa = 10.06 SCEE167 pKa = 3.93 FHH169 pKa = 7.55 RR170 pKa = 11.84 KK171 pKa = 6.24 NTGVKK176 pKa = 9.89 EE177 pKa = 4.01 LLCSLCYY184 pKa = 9.86 MRR186 pKa = 11.84 AHH188 pKa = 6.47 CHH190 pKa = 6.56 FIYY193 pKa = 10.49 SKK195 pKa = 10.92 CVV197 pKa = 2.88
Molecular weight: 21.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.088
IPC2_protein 4.279
IPC_protein 4.228
Toseland 4.062
ProMoST 4.329
Dawson 4.177
Bjellqvist 4.317
Wikipedia 4.037
Rodwell 4.062
Grimsley 3.973
Solomon 4.164
Lehninger 4.126
Nozaki 4.279
DTASelect 4.418
Thurlkill 4.075
EMBOSS 4.062
Sillero 4.329
Patrickios 2.626
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.228
Protein with the highest isoelectric point:
>tr|A0A1W6HVP8|A0A1W6HVP8_9ADEN Early E1A protein OS=Human adenovirus sp. OX=1907210 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.43 RR4 pKa = 11.84 KK5 pKa = 9.85 YY6 pKa = 9.79 KK7 pKa = 10.9 EE8 pKa = 3.54 EE9 pKa = 3.89 MLQVIAPEE17 pKa = 4.73 VYY19 pKa = 9.76 GQPLKK24 pKa = 10.9 DD25 pKa = 3.29 EE26 pKa = 4.48 KK27 pKa = 10.91 KK28 pKa = 8.53 PRR30 pKa = 11.84 KK31 pKa = 9.09 IKK33 pKa = 10.18 RR34 pKa = 11.84 AKK36 pKa = 9.47 KK37 pKa = 9.74 DD38 pKa = 3.34 KK39 pKa = 10.97 KK40 pKa = 10.65 EE41 pKa = 4.15 EE42 pKa = 3.99 EE43 pKa = 4.72 DD44 pKa = 4.04 GDD46 pKa = 4.23 DD47 pKa = 3.73 GLAEE51 pKa = 4.4 FVRR54 pKa = 11.84 EE55 pKa = 3.86 FAPRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 VQWRR65 pKa = 11.84 GRR67 pKa = 11.84 KK68 pKa = 8.6 VRR70 pKa = 11.84 HH71 pKa = 5.92 LLRR74 pKa = 11.84 PGTSVVFTPGEE85 pKa = 3.87 RR86 pKa = 11.84 SSATFKK92 pKa = 10.65 RR93 pKa = 11.84 SYY95 pKa = 11.27 DD96 pKa = 3.53 EE97 pKa = 5.21 VYY99 pKa = 11.03 GDD101 pKa = 5.76 DD102 pKa = 6.37 DD103 pKa = 4.14 ILEE106 pKa = 4.15 QAADD110 pKa = 3.8 RR111 pKa = 11.84 LGEE114 pKa = 3.76 FAYY117 pKa = 10.26 GKK119 pKa = 10.08 RR120 pKa = 11.84 SRR122 pKa = 11.84 INPKK126 pKa = 10.42 DD127 pKa = 3.46 EE128 pKa = 4.27 TVSIPLDD135 pKa = 3.78 HH136 pKa = 7.08 GNPTPSLKK144 pKa = 10.31 PVTLQQVLPVTPRR157 pKa = 11.84 TGVKK161 pKa = 10.0 RR162 pKa = 11.84 EE163 pKa = 4.06 GEE165 pKa = 3.96 DD166 pKa = 4.36 LYY168 pKa = 10.28 PTMQLMVPKK177 pKa = 9.82 RR178 pKa = 11.84 QKK180 pKa = 11.1 LEE182 pKa = 3.99 DD183 pKa = 3.51 VLEE186 pKa = 4.25 KK187 pKa = 11.2 VKK189 pKa = 10.61 VDD191 pKa = 3.69 PDD193 pKa = 3.28 IQPEE197 pKa = 4.48 VKK199 pKa = 9.97 VRR201 pKa = 11.84 PIKK204 pKa = 10.32 QVAPGLGVQTVDD216 pKa = 2.93 IKK218 pKa = 11.14 IPTEE222 pKa = 3.9 SMEE225 pKa = 4.38 VQTEE229 pKa = 4.07 PAKK232 pKa = 9.51 PTATSTEE239 pKa = 4.22 VQTDD243 pKa = 2.89 PWMPMPITTDD253 pKa = 2.91 AAGPTRR259 pKa = 11.84 RR260 pKa = 11.84 SRR262 pKa = 11.84 RR263 pKa = 11.84 KK264 pKa = 9.41 YY265 pKa = 10.39 GPASLLMPNYY275 pKa = 9.18 VVHH278 pKa = 6.52 PSIIPTPGYY287 pKa = 9.95 RR288 pKa = 11.84 GTRR291 pKa = 11.84 YY292 pKa = 9.37 YY293 pKa = 10.8 RR294 pKa = 11.84 SRR296 pKa = 11.84 NSTSRR301 pKa = 11.84 RR302 pKa = 11.84 RR303 pKa = 11.84 RR304 pKa = 11.84 KK305 pKa = 7.96 TPANRR310 pKa = 11.84 SRR312 pKa = 11.84 RR313 pKa = 11.84 RR314 pKa = 11.84 RR315 pKa = 11.84 RR316 pKa = 11.84 TSKK319 pKa = 8.65 PTPGALVRR327 pKa = 11.84 QVYY330 pKa = 9.83 RR331 pKa = 11.84 NSSAEE336 pKa = 4.0 PLTLPRR342 pKa = 11.84 ARR344 pKa = 11.84 YY345 pKa = 8.46 HH346 pKa = 6.34 PSIITT351 pKa = 3.35
Molecular weight: 40.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 9.56
IPC_protein 9.897
Toseland 10.452
ProMoST 10.058
Dawson 10.57
Bjellqvist 10.218
Wikipedia 10.745
Rodwell 10.95
Grimsley 10.628
Solomon 10.628
Lehninger 10.599
Nozaki 10.409
DTASelect 10.218
Thurlkill 10.452
EMBOSS 10.833
Sillero 10.482
Patrickios 10.613
IPC_peptide 10.628
IPC2_peptide 8.77
IPC2.peptide.svr19 8.788
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
3
27
8516
54
1122
315.4
35.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.858 ± 0.329
1.973 ± 0.416
5.202 ± 0.197
6.247 ± 0.406
3.84 ± 0.243
6.118 ± 0.286
2.302 ± 0.238
4.357 ± 0.305
4.556 ± 0.393
9.382 ± 0.498
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.994 ± 0.255
4.979 ± 0.451
6.329 ± 0.36
4.439 ± 0.287
6.353 ± 0.413
6.881 ± 0.33
6.165 ± 0.457
6.118 ± 0.351
1.327 ± 0.131
3.581 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here