Cardinium endosymbiont of Dermatophagoides farinae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Amoebophilaceae; Candidatus Cardinium; unclassified Candidatus Cardinium

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A556QWR9|A0A556QWR9_9BACT Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cardinium endosymbiont of Dermatophagoides farinae OX=2597823 GN=gatA PE=3 SV=1
MM1 pKa = 7.24YY2 pKa = 7.76WTLEE6 pKa = 4.53LISHH10 pKa = 7.04LEE12 pKa = 3.97DD13 pKa = 4.46APWPAIKK20 pKa = 10.51DD21 pKa = 3.71EE22 pKa = 5.17LIDD25 pKa = 3.55YY26 pKa = 8.44AKK28 pKa = 10.6RR29 pKa = 11.84SGAPFEE35 pKa = 4.54VIRR38 pKa = 11.84NLEE41 pKa = 3.99EE42 pKa = 6.08LDD44 pKa = 4.72DD45 pKa = 5.89DD46 pKa = 5.05DD47 pKa = 5.43YY48 pKa = 11.54PYY50 pKa = 11.31NSPEE54 pKa = 4.61EE55 pKa = 3.98IWPDD59 pKa = 3.69YY60 pKa = 8.44PTGDD64 pKa = 3.16DD65 pKa = 3.85FVFNEE70 pKa = 4.67DD71 pKa = 3.35EE72 pKa = 4.34YY73 pKa = 11.97

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A556QXD1|A0A556QXD1_9BACT Uncharacterized protein OS=Cardinium endosymbiont of Dermatophagoides farinae OX=2597823 GN=FPG78_04875 PE=3 SV=1
MM1 pKa = 7.14VKK3 pKa = 10.14RR4 pKa = 11.84YY5 pKa = 6.83TCARR9 pKa = 11.84VGLLIGLLSLNILSACVHH27 pKa = 5.69TRR29 pKa = 11.84QPFIGRR35 pKa = 11.84DD36 pKa = 3.09PAIRR40 pKa = 11.84WDD42 pKa = 3.6YY43 pKa = 7.58TQRR46 pKa = 11.84DD47 pKa = 3.51YY48 pKa = 10.35TQRR51 pKa = 11.84YY52 pKa = 6.43EE53 pKa = 3.82QDD55 pKa = 2.68TGIGDD60 pKa = 3.65KK61 pKa = 10.76FKK63 pKa = 10.64IIVPFTFLILWVTLFPAWIIKK84 pKa = 8.01MSPNTNSSSGFNRR97 pKa = 11.84TGLHH101 pKa = 6.26GEE103 pKa = 4.11DD104 pKa = 3.33AFFTKK109 pKa = 10.21GVRR112 pKa = 11.84PFMFDD117 pKa = 3.05PVRR120 pKa = 11.84RR121 pKa = 11.84SIPNEE126 pKa = 3.51RR127 pKa = 11.84LGNSSVIPVKK137 pKa = 10.54KK138 pKa = 10.42LNRR141 pKa = 11.84FQKK144 pKa = 9.27TLALAVDD151 pKa = 4.16KK152 pKa = 10.76FSAGRR157 pKa = 11.84RR158 pKa = 11.84TQSNMMPP165 pKa = 3.81

Molecular weight: 18.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1187

0

1187

330757

21

1420

278.6

31.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.195 ± 0.071

1.465 ± 0.03

4.949 ± 0.056

5.538 ± 0.068

4.507 ± 0.058

5.701 ± 0.056

2.624 ± 0.035

7.882 ± 0.068

7.73 ± 0.077

10.731 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.033

4.697 ± 0.064

3.767 ± 0.044

4.198 ± 0.05

4.063 ± 0.045

6.578 ± 0.073

5.35 ± 0.047

5.669 ± 0.054

1.008 ± 0.024

4.046 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski