Alteromonas phage XX1924
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9IZR5|A0A6B9IZR5_9CAUD Uncharacterized protein OS=Alteromonas phage XX1924 OX=2664192 PE=4 SV=1
MM1 pKa = 7.57 SNTVYY6 pKa = 10.38 IGPAEE11 pKa = 4.16 CHH13 pKa = 6.42 PDD15 pKa = 3.49 VTEE18 pKa = 4.38 MPASVAISPRR28 pKa = 11.84 EE29 pKa = 3.79 VLIEE33 pKa = 4.04 SSGEE37 pKa = 3.83 FAPAGADD44 pKa = 3.17 AAGVVYY50 pKa = 9.43 FALEE54 pKa = 4.36 NILGEE59 pKa = 4.2 VTEE62 pKa = 5.53 DD63 pKa = 3.39 YY64 pKa = 9.78 TAGEE68 pKa = 4.69 TVQGARR74 pKa = 11.84 PKK76 pKa = 10.29 SGEE79 pKa = 4.12 YY80 pKa = 10.69 YY81 pKa = 10.43 EE82 pKa = 4.46 LTLAASQTIAKK93 pKa = 9.59 DD94 pKa = 3.19 AALTTDD100 pKa = 3.23 ASGHH104 pKa = 5.42 LVALGAGSNTIAYY117 pKa = 9.61 ADD119 pKa = 3.81 EE120 pKa = 4.67 AVTTTGATGSIRR132 pKa = 11.84 VYY134 pKa = 11.09 VKK136 pKa = 10.77
Molecular weight: 13.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.963
IPC2_protein 4.253
IPC_protein 4.126
Toseland 3.973
ProMoST 4.24
Dawson 4.075
Bjellqvist 4.228
Wikipedia 3.948
Rodwell 3.973
Grimsley 3.884
Solomon 4.062
Lehninger 4.024
Nozaki 4.19
DTASelect 4.304
Thurlkill 3.986
EMBOSS 3.961
Sillero 4.24
Patrickios 0.947
IPC_peptide 4.075
IPC2_peptide 4.228
IPC2.peptide.svr19 4.139
Protein with the highest isoelectric point:
>tr|A0A6B9IZU1|A0A6B9IZU1_9CAUD Uncharacterized protein OS=Alteromonas phage XX1924 OX=2664192 PE=3 SV=1
MM1 pKa = 7.04 THH3 pKa = 6.77 TEE5 pKa = 3.99 AQLHH9 pKa = 5.7 ISKK12 pKa = 10.23 ARR14 pKa = 11.84 KK15 pKa = 7.46 QSKK18 pKa = 8.94 FFVIVRR24 pKa = 11.84 TDD26 pKa = 3.26 ALRR29 pKa = 11.84 LAGYY33 pKa = 9.24 VQLCGAGYY41 pKa = 7.92 LTLVHH46 pKa = 6.69 RR47 pKa = 11.84 HH48 pKa = 5.43 LLL50 pKa = 3.78
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.288
IPC2_protein 9.648
IPC_protein 10.072
Toseland 10.321
ProMoST 9.999
Dawson 10.482
Bjellqvist 10.175
Wikipedia 10.672
Rodwell 10.847
Grimsley 10.555
Solomon 10.555
Lehninger 10.526
Nozaki 10.321
DTASelect 10.16
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.409
Patrickios 10.716
IPC_peptide 10.555
IPC2_peptide 9.151
IPC2.peptide.svr19 8.496
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
11928
42
660
186.4
20.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.451 ± 0.452
1.367 ± 0.164
6.053 ± 0.262
6.841 ± 0.323
3.848 ± 0.22
7.218 ± 0.313
1.953 ± 0.22
6.355 ± 0.218
6.883 ± 0.466
7.755 ± 0.317
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.211
4.795 ± 0.256
3.471 ± 0.317
3.965 ± 0.27
4.921 ± 0.271
7.042 ± 0.357
5.449 ± 0.317
5.986 ± 0.248
1.484 ± 0.132
3.421 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here