Haloredivivus sp. (strain G17)
Average proteome isoelectric point is 5.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2152 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H0AAF9|H0AAF9_HALSG Uncharacterized protein OS=Haloredivivus sp. (strain G17) OX=1072681 GN=HRED_05089 PE=4 SV=1
MM1 pKa = 7.57 IKK3 pKa = 10.45 LLRR6 pKa = 11.84 LRR8 pKa = 11.84 RR9 pKa = 11.84 PIVGEE14 pKa = 3.78 IAIKK18 pKa = 10.24 RR19 pKa = 11.84 YY20 pKa = 9.37 FLTLLSILLFISPVMAQTYY39 pKa = 10.38 DD40 pKa = 3.55 FNNCGSLGKK49 pKa = 9.92 NGPSSCSYY57 pKa = 10.24 TGDD60 pKa = 3.11 NSVVINNQGFQKK72 pKa = 8.53 WTVPEE77 pKa = 4.05 SGKK80 pKa = 10.33 YY81 pKa = 9.39 RR82 pKa = 11.84 IKK84 pKa = 10.91 AAGAQGAAGDD94 pKa = 3.59 PSYY97 pKa = 10.99 RR98 pKa = 11.84 GGYY101 pKa = 8.22 GALVKK106 pKa = 10.87 GSFNLRR112 pKa = 11.84 EE113 pKa = 4.3 GEE115 pKa = 4.43 DD116 pKa = 3.13 IVIAVGQKK124 pKa = 8.44 GTAGSPSTNDD134 pKa = 2.94 NDD136 pKa = 4.12 GGGGGGSFVVSSSDD150 pKa = 3.41 YY151 pKa = 10.11 TSASTSDD158 pKa = 3.11 ILVIAGGGAGTRR170 pKa = 11.84 AYY172 pKa = 11.12 VNDD175 pKa = 3.69 NGCPGNEE182 pKa = 3.62 GEE184 pKa = 4.38 YY185 pKa = 10.29 AIEE188 pKa = 4.8 GSGSDD193 pKa = 3.5 QTSSCPVKK201 pKa = 10.5 STDD204 pKa = 3.52 LGEE207 pKa = 4.34 GGIVSSGSWGSAGGGWGSNGEE228 pKa = 3.99 GDD230 pKa = 4.08 YY231 pKa = 10.07 DD232 pKa = 3.76 TSAGGRR238 pKa = 11.84 SWFNGLEE245 pKa = 4.23 GGHH248 pKa = 6.73 DD249 pKa = 4.02 SEE251 pKa = 5.76 SPPAPGGFGAGGSGHH266 pKa = 6.35 GSWGGGGGGGYY277 pKa = 10.02 SGGDD281 pKa = 3.51 GGRR284 pKa = 11.84 VAGGGGSYY292 pKa = 8.84 NTGSDD297 pKa = 3.24 QSNTGGEE304 pKa = 4.29 NSGHH308 pKa = 6.24 GYY310 pKa = 10.2 VEE312 pKa = 4.16 IEE314 pKa = 4.39 TVAVTSDD321 pKa = 3.47 PTVQSPTYY329 pKa = 8.94 TDD331 pKa = 3.32 SQATSSSPASFEE343 pKa = 3.26 VDD345 pKa = 2.96 APVDD349 pKa = 3.14 VDD351 pKa = 3.64 TSVDD355 pKa = 3.46 GGNSLSSCSVDD366 pKa = 3.39 VTGDD370 pKa = 3.77 DD371 pKa = 3.61 NGGSYY376 pKa = 10.65 SPSTTVSGDD385 pKa = 3.11 TCEE388 pKa = 4.92 FSVDD392 pKa = 4.34 NNDD395 pKa = 3.8 DD396 pKa = 4.37 SNWEE400 pKa = 3.91 PGEE403 pKa = 4.22 TVSVDD408 pKa = 2.86 ITATDD413 pKa = 3.66 DD414 pKa = 4.53 LGNTDD419 pKa = 2.93 VGYY422 pKa = 7.86 FTHH425 pKa = 7.01 TFDD428 pKa = 4.49 NTPPTINSVTPSDD441 pKa = 3.87 GASSVWGGEE450 pKa = 3.96 NGVSVQADD458 pKa = 3.58 VVDD461 pKa = 4.83 FEE463 pKa = 5.25 SVHH466 pKa = 6.18 TNDD469 pKa = 4.74 DD470 pKa = 3.68 LTVDD474 pKa = 4.83 FVDD477 pKa = 5.4 DD478 pKa = 3.84 SLGQFGSVTVSNGDD492 pKa = 3.07 GTASVNWEE500 pKa = 3.9 RR501 pKa = 11.84 NGQQDD506 pKa = 3.03 MFGFDD511 pKa = 3.29 RR512 pKa = 11.84 GTSYY516 pKa = 10.74 GWKK519 pKa = 10.28 VNVDD523 pKa = 3.78 DD524 pKa = 4.41 VTASTTTQSQSFTVLSEE541 pKa = 4.09 ASTSNPSPTGGDD553 pKa = 3.06 SADD556 pKa = 3.58 QMKK559 pKa = 10.56 VDD561 pKa = 3.83 IDD563 pKa = 3.86 NPAVSNDD570 pKa = 2.97 GSLSGSTVEE579 pKa = 4.49 FWVDD583 pKa = 3.37 DD584 pKa = 3.93 GLEE587 pKa = 4.2 VYY589 pKa = 9.64 QVNTDD594 pKa = 3.62 TVSGSSGTASISLQDD609 pKa = 3.35 FEE611 pKa = 5.5 FRR613 pKa = 11.84 NEE615 pKa = 4.04 PADD618 pKa = 3.69 GTGDD622 pKa = 3.19 WFVKK626 pKa = 10.78 VNDD629 pKa = 4.37 TYY631 pKa = 11.79 SEE633 pKa = 4.05 TDD635 pKa = 3.39 YY636 pKa = 11.59 SRR638 pKa = 11.84 SKK640 pKa = 10.95 YY641 pKa = 10.67 GSNTYY646 pKa = 10.38 NFSAAKK652 pKa = 9.48 PPLILSEE659 pKa = 5.02 DD660 pKa = 3.78 PSSYY664 pKa = 10.45 PIAEE668 pKa = 4.31 TGVGG672 pKa = 3.41
Molecular weight: 68.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|H0ADY3|H0ADY3_HALSG Metalloenzyme domain-containing protein OS=Haloredivivus sp. (strain G17) OX=1072681 GN=HRED_10157 PE=4 SV=1
MM1 pKa = 7.58 RR2 pKa = 11.84 KK3 pKa = 9.07 VRR5 pKa = 11.84 RR6 pKa = 11.84 LKK8 pKa = 9.76 KK9 pKa = 9.11 TSLKK13 pKa = 10.6 RR14 pKa = 11.84 NLGKK18 pKa = 9.98 MSQNKK23 pKa = 8.77 MNLKK27 pKa = 8.93 VKK29 pKa = 10.02 AWRR32 pKa = 11.84 VV33 pKa = 3.23
Molecular weight: 4.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 10.921
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.574
Grimsley 12.676
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.31
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.985
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2152
0
2152
324344
20
1684
150.7
17.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.939 ± 0.059
0.656 ± 0.021
6.901 ± 0.073
11.257 ± 0.115
4.104 ± 0.045
6.864 ± 0.069
1.59 ± 0.029
6.617 ± 0.06
6.176 ± 0.084
8.595 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.591 ± 0.036
4.423 ± 0.056
3.304 ± 0.041
3.343 ± 0.043
4.869 ± 0.054
7.133 ± 0.068
4.856 ± 0.055
6.599 ± 0.061
0.864 ± 0.018
3.24 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here