Haloredivivus sp. (strain G17)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Nanohaloarchaea; Candidatus Haloredivivus

Average proteome isoelectric point is 5.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0AAF9|H0AAF9_HALSG Uncharacterized protein OS=Haloredivivus sp. (strain G17) OX=1072681 GN=HRED_05089 PE=4 SV=1
MM1 pKa = 7.57IKK3 pKa = 10.45LLRR6 pKa = 11.84LRR8 pKa = 11.84RR9 pKa = 11.84PIVGEE14 pKa = 3.78IAIKK18 pKa = 10.24RR19 pKa = 11.84YY20 pKa = 9.37FLTLLSILLFISPVMAQTYY39 pKa = 10.38DD40 pKa = 3.55FNNCGSLGKK49 pKa = 9.92NGPSSCSYY57 pKa = 10.24TGDD60 pKa = 3.11NSVVINNQGFQKK72 pKa = 8.53WTVPEE77 pKa = 4.05SGKK80 pKa = 10.33YY81 pKa = 9.39RR82 pKa = 11.84IKK84 pKa = 10.91AAGAQGAAGDD94 pKa = 3.59PSYY97 pKa = 10.99RR98 pKa = 11.84GGYY101 pKa = 8.22GALVKK106 pKa = 10.87GSFNLRR112 pKa = 11.84EE113 pKa = 4.3GEE115 pKa = 4.43DD116 pKa = 3.13IVIAVGQKK124 pKa = 8.44GTAGSPSTNDD134 pKa = 2.94NDD136 pKa = 4.12GGGGGGSFVVSSSDD150 pKa = 3.41YY151 pKa = 10.11TSASTSDD158 pKa = 3.11ILVIAGGGAGTRR170 pKa = 11.84AYY172 pKa = 11.12VNDD175 pKa = 3.69NGCPGNEE182 pKa = 3.62GEE184 pKa = 4.38YY185 pKa = 10.29AIEE188 pKa = 4.8GSGSDD193 pKa = 3.5QTSSCPVKK201 pKa = 10.5STDD204 pKa = 3.52LGEE207 pKa = 4.34GGIVSSGSWGSAGGGWGSNGEE228 pKa = 3.99GDD230 pKa = 4.08YY231 pKa = 10.07DD232 pKa = 3.76TSAGGRR238 pKa = 11.84SWFNGLEE245 pKa = 4.23GGHH248 pKa = 6.73DD249 pKa = 4.02SEE251 pKa = 5.76SPPAPGGFGAGGSGHH266 pKa = 6.35GSWGGGGGGGYY277 pKa = 10.02SGGDD281 pKa = 3.51GGRR284 pKa = 11.84VAGGGGSYY292 pKa = 8.84NTGSDD297 pKa = 3.24QSNTGGEE304 pKa = 4.29NSGHH308 pKa = 6.24GYY310 pKa = 10.2VEE312 pKa = 4.16IEE314 pKa = 4.39TVAVTSDD321 pKa = 3.47PTVQSPTYY329 pKa = 8.94TDD331 pKa = 3.32SQATSSSPASFEE343 pKa = 3.26VDD345 pKa = 2.96APVDD349 pKa = 3.14VDD351 pKa = 3.64TSVDD355 pKa = 3.46GGNSLSSCSVDD366 pKa = 3.39VTGDD370 pKa = 3.77DD371 pKa = 3.61NGGSYY376 pKa = 10.65SPSTTVSGDD385 pKa = 3.11TCEE388 pKa = 4.92FSVDD392 pKa = 4.34NNDD395 pKa = 3.8DD396 pKa = 4.37SNWEE400 pKa = 3.91PGEE403 pKa = 4.22TVSVDD408 pKa = 2.86ITATDD413 pKa = 3.66DD414 pKa = 4.53LGNTDD419 pKa = 2.93VGYY422 pKa = 7.86FTHH425 pKa = 7.01TFDD428 pKa = 4.49NTPPTINSVTPSDD441 pKa = 3.87GASSVWGGEE450 pKa = 3.96NGVSVQADD458 pKa = 3.58VVDD461 pKa = 4.83FEE463 pKa = 5.25SVHH466 pKa = 6.18TNDD469 pKa = 4.74DD470 pKa = 3.68LTVDD474 pKa = 4.83FVDD477 pKa = 5.4DD478 pKa = 3.84SLGQFGSVTVSNGDD492 pKa = 3.07GTASVNWEE500 pKa = 3.9RR501 pKa = 11.84NGQQDD506 pKa = 3.03MFGFDD511 pKa = 3.29RR512 pKa = 11.84GTSYY516 pKa = 10.74GWKK519 pKa = 10.28VNVDD523 pKa = 3.78DD524 pKa = 4.41VTASTTTQSQSFTVLSEE541 pKa = 4.09ASTSNPSPTGGDD553 pKa = 3.06SADD556 pKa = 3.58QMKK559 pKa = 10.56VDD561 pKa = 3.83IDD563 pKa = 3.86NPAVSNDD570 pKa = 2.97GSLSGSTVEE579 pKa = 4.49FWVDD583 pKa = 3.37DD584 pKa = 3.93GLEE587 pKa = 4.2VYY589 pKa = 9.64QVNTDD594 pKa = 3.62TVSGSSGTASISLQDD609 pKa = 3.35FEE611 pKa = 5.5FRR613 pKa = 11.84NEE615 pKa = 4.04PADD618 pKa = 3.69GTGDD622 pKa = 3.19WFVKK626 pKa = 10.78VNDD629 pKa = 4.37TYY631 pKa = 11.79SEE633 pKa = 4.05TDD635 pKa = 3.39YY636 pKa = 11.59SRR638 pKa = 11.84SKK640 pKa = 10.95YY641 pKa = 10.67GSNTYY646 pKa = 10.38NFSAAKK652 pKa = 9.48PPLILSEE659 pKa = 5.02DD660 pKa = 3.78PSSYY664 pKa = 10.45PIAEE668 pKa = 4.31TGVGG672 pKa = 3.41

Molecular weight: 68.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0ADY3|H0ADY3_HALSG Metalloenzyme domain-containing protein OS=Haloredivivus sp. (strain G17) OX=1072681 GN=HRED_10157 PE=4 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84KK3 pKa = 9.07VRR5 pKa = 11.84RR6 pKa = 11.84LKK8 pKa = 9.76KK9 pKa = 9.11TSLKK13 pKa = 10.6RR14 pKa = 11.84NLGKK18 pKa = 9.98MSQNKK23 pKa = 8.77MNLKK27 pKa = 8.93VKK29 pKa = 10.02AWRR32 pKa = 11.84VV33 pKa = 3.23

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2152

0

2152

324344

20

1684

150.7

17.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.939 ± 0.059

0.656 ± 0.021

6.901 ± 0.073

11.257 ± 0.115

4.104 ± 0.045

6.864 ± 0.069

1.59 ± 0.029

6.617 ± 0.06

6.176 ± 0.084

8.595 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.036

4.423 ± 0.056

3.304 ± 0.041

3.343 ± 0.043

4.869 ± 0.054

7.133 ± 0.068

4.856 ± 0.055

6.599 ± 0.061

0.864 ± 0.018

3.24 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski