Amino acid dipepetide frequency for Haloredivivus sp. (strain G17)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.323AlaAla: 4.323 ± 0.145
0.447AlaCys: 0.447 ± 0.037
3.527AlaAsp: 3.527 ± 0.107
6.9AlaGlu: 6.9 ± 0.164
2.544AlaPhe: 2.544 ± 0.089
4.239AlaGly: 4.239 ± 0.129
0.845AlaHis: 0.845 ± 0.055
3.388AlaIle: 3.388 ± 0.128
2.753AlaLys: 2.753 ± 0.093
5.775AlaLeu: 5.775 ± 0.146
1.273AlaMet: 1.273 ± 0.06
1.945AlaAsn: 1.945 ± 0.081
1.603AlaPro: 1.603 ± 0.067
1.939AlaGln: 1.939 ± 0.081
2.658AlaArg: 2.658 ± 0.097
5.096AlaSer: 5.096 ± 0.137
2.337AlaThr: 2.337 ± 0.095
4.989AlaVal: 4.989 ± 0.134
0.561AlaTrp: 0.561 ± 0.038
2.223AlaTyr: 2.223 ± 0.075
0.025AlaXaa: 0.025 ± 0.009
Cys
0.234CysAla: 0.234 ± 0.023
0.092CysCys: 0.092 ± 0.016
0.456CysAsp: 0.456 ± 0.039
0.601CysGlu: 0.601 ± 0.049
0.376CysPhe: 0.376 ± 0.031
0.681CysGly: 0.681 ± 0.049
0.123CysHis: 0.123 ± 0.018
0.311CysIle: 0.311 ± 0.029
0.382CysLys: 0.382 ± 0.032
0.429CysLeu: 0.429 ± 0.039
0.176CysMet: 0.176 ± 0.023
0.253CysAsn: 0.253 ± 0.028
0.392CysPro: 0.392 ± 0.037
0.219CysGln: 0.219 ± 0.026
0.419CysArg: 0.419 ± 0.038
0.49CysSer: 0.49 ± 0.044
0.327CysThr: 0.327 ± 0.035
0.281CysVal: 0.281 ± 0.028
0.123CysTrp: 0.123 ± 0.021
0.191CysTyr: 0.191 ± 0.022
0.003CysXaa: 0.003 ± 0.003
Asp
3.802AspAla: 3.802 ± 0.11
0.422AspCys: 0.422 ± 0.04
3.814AspAsp: 3.814 ± 0.143
8.55AspGlu: 8.55 ± 0.213
3.496AspPhe: 3.496 ± 0.106
4.064AspGly: 4.064 ± 0.139
1.107AspHis: 1.107 ± 0.066
4.952AspIle: 4.952 ± 0.118
3.669AspLys: 3.669 ± 0.122
6.579AspLeu: 6.579 ± 0.161
1.757AspMet: 1.757 ± 0.069
2.784AspAsn: 2.784 ± 0.099
2.211AspPro: 2.211 ± 0.09
2.306AspGln: 2.306 ± 0.091
3.768AspArg: 3.768 ± 0.123
4.708AspSer: 4.708 ± 0.148
3.089AspThr: 3.089 ± 0.102
4.397AspVal: 4.397 ± 0.127
0.863AspTrp: 0.863 ± 0.051
2.642AspTyr: 2.642 ± 0.098
0.028AspXaa: 0.028 ± 0.009
Glu
7.612GluAla: 7.612 ± 0.184
0.546GluCys: 0.546 ± 0.041
8.836GluAsp: 8.836 ± 0.195
14.093GluGlu: 14.093 ± 0.345
4.224GluPhe: 4.224 ± 0.12
6.354GluGly: 6.354 ± 0.16
1.548GluHis: 1.548 ± 0.083
8.349GluIle: 8.349 ± 0.158
9.857GluLys: 9.857 ± 0.241
8.621GluLeu: 8.621 ± 0.17
3.083GluMet: 3.083 ± 0.111
6.361GluAsn: 6.361 ± 0.157
2.396GluPro: 2.396 ± 0.101
2.929GluGln: 2.929 ± 0.112
4.81GluArg: 4.81 ± 0.13
6.521GluSer: 6.521 ± 0.185
4.911GluThr: 4.911 ± 0.134
7.295GluVal: 7.295 ± 0.161
0.922GluTrp: 0.922 ± 0.053
3.228GluTyr: 3.228 ± 0.097
0.074GluXaa: 0.074 ± 0.016
Phe
1.989PheAla: 1.989 ± 0.074
0.388PheCys: 0.388 ± 0.035
2.867PheAsp: 2.867 ± 0.102
4.332PheGlu: 4.332 ± 0.109
1.912PhePhe: 1.912 ± 0.097
2.759PheGly: 2.759 ± 0.093
0.62PheHis: 0.62 ± 0.047
2.735PheIle: 2.735 ± 0.106
1.878PheLys: 1.878 ± 0.068
3.755PheLeu: 3.755 ± 0.126
1.27PheMet: 1.27 ± 0.068
1.686PheAsn: 1.686 ± 0.076
1.27PhePro: 1.27 ± 0.056
1.591PheGln: 1.591 ± 0.067
1.868PheArg: 1.868 ± 0.077
3.487PheSer: 3.487 ± 0.13
2.177PheThr: 2.177 ± 0.097
2.272PheVal: 2.272 ± 0.091
0.453PheTrp: 0.453 ± 0.037
1.693PheTyr: 1.693 ± 0.067
0.037PheXaa: 0.037 ± 0.011
Gly
3.506GlyAla: 3.506 ± 0.116
0.398GlyCys: 0.398 ± 0.036
4.64GlyAsp: 4.64 ± 0.135
7.227GlyGlu: 7.227 ± 0.175
3.561GlyPhe: 3.561 ± 0.11
4.656GlyGly: 4.656 ± 0.167
1.039GlyHis: 1.039 ± 0.059
4.252GlyIle: 4.252 ± 0.137
3.925GlyLys: 3.925 ± 0.122
8.26GlyLeu: 8.26 ± 0.163
1.875GlyMet: 1.875 ± 0.071
2.716GlyAsn: 2.716 ± 0.096
1.819GlyPro: 1.819 ± 0.083
2.044GlyGln: 2.044 ± 0.088
3.478GlyArg: 3.478 ± 0.118
4.689GlySer: 4.689 ± 0.16
3.21GlyThr: 3.21 ± 0.101
4.205GlyVal: 4.205 ± 0.11
0.632GlyTrp: 0.632 ± 0.044
2.482GlyTyr: 2.482 ± 0.082
0.031GlyXaa: 0.031 ± 0.009
His
0.832HisAla: 0.832 ± 0.05
0.145HisCys: 0.145 ± 0.023
0.99HisAsp: 0.99 ± 0.057
1.548HisGlu: 1.548 ± 0.074
0.703HisPhe: 0.703 ± 0.046
1.027HisGly: 1.027 ± 0.061
0.45HisHis: 0.45 ± 0.045
0.993HisIle: 0.993 ± 0.049
0.78HisLys: 0.78 ± 0.047
1.563HisLeu: 1.563 ± 0.061
0.302HisMet: 0.302 ± 0.029
0.552HisAsn: 0.552 ± 0.043
0.737HisPro: 0.737 ± 0.048
0.657HisGln: 0.657 ± 0.043
0.91HisArg: 0.91 ± 0.053
1.196HisSer: 1.196 ± 0.059
0.762HisThr: 0.762 ± 0.051
1.021HisVal: 1.021 ± 0.06
0.117HisTrp: 0.117 ± 0.02
0.61HisTyr: 0.61 ± 0.044
0.009HisXaa: 0.009 ± 0.005
Ile
4.131IleAla: 4.131 ± 0.147
0.324IleCys: 0.324 ± 0.031
4.807IleAsp: 4.807 ± 0.112
8.426IleGlu: 8.426 ± 0.194
2.51IlePhe: 2.51 ± 0.098
5.183IleGly: 5.183 ± 0.146
1.295IleHis: 1.295 ± 0.061
3.857IleIle: 3.857 ± 0.125
3.197IleLys: 3.197 ± 0.106
5.614IleLeu: 5.614 ± 0.154
1.36IleMet: 1.36 ± 0.066
2.652IleAsn: 2.652 ± 0.101
2.365IlePro: 2.365 ± 0.083
2.365IleGln: 2.365 ± 0.086
2.877IleArg: 2.877 ± 0.095
5.365IleSer: 5.365 ± 0.135
3.361IleThr: 3.361 ± 0.105
4.091IleVal: 4.091 ± 0.118
0.385IleTrp: 0.385 ± 0.031
1.979IleTyr: 1.979 ± 0.084
0.028IleXaa: 0.028 ± 0.009
Lys
4.492LysAla: 4.492 ± 0.138
0.453LysCys: 0.453 ± 0.041
4.079LysAsp: 4.079 ± 0.113
6.422LysGlu: 6.422 ± 0.179
2.177LysPhe: 2.177 ± 0.094
3.903LysGly: 3.903 ± 0.132
1.119LysHis: 1.119 ± 0.065
5.315LysIle: 5.315 ± 0.164
4.739LysLys: 4.739 ± 0.152
4.865LysLeu: 4.865 ± 0.13
1.714LysMet: 1.714 ± 0.083
2.975LysAsn: 2.975 ± 0.098
1.767LysPro: 1.767 ± 0.075
1.868LysGln: 1.868 ± 0.081
3.012LysArg: 3.012 ± 0.106
3.401LysSer: 3.401 ± 0.105
2.947LysThr: 2.947 ± 0.087
4.212LysVal: 4.212 ± 0.12
0.496LysTrp: 0.496 ± 0.037
1.739LysTyr: 1.739 ± 0.08
0.062LysXaa: 0.062 ± 0.013
Leu
4.939LeuAla: 4.939 ± 0.13
0.496LeuCys: 0.496 ± 0.047
6.268LeuAsp: 6.268 ± 0.136
10.563LeuGlu: 10.563 ± 0.213
3.095LeuPhe: 3.095 ± 0.109
6.391LeuGly: 6.391 ± 0.176
1.246LeuHis: 1.246 ± 0.067
5.513LeuIle: 5.513 ± 0.136
5.648LeuLys: 5.648 ± 0.175
6.623LeuLeu: 6.623 ± 0.213
2.34LeuMet: 2.34 ± 0.097
4.098LeuAsn: 4.098 ± 0.127
2.648LeuPro: 2.648 ± 0.091
2.8LeuGln: 2.8 ± 0.097
4.039LeuArg: 4.039 ± 0.096
5.972LeuSer: 5.972 ± 0.168
4.471LeuThr: 4.471 ± 0.111
5.534LeuVal: 5.534 ± 0.143
0.617LeuTrp: 0.617 ± 0.044
2.599LeuTyr: 2.599 ± 0.099
0.046LeuXaa: 0.046 ± 0.012
Met
1.585MetAla: 1.585 ± 0.074
0.136MetCys: 0.136 ± 0.018
1.819MetAsp: 1.819 ± 0.081
2.241MetGlu: 2.241 ± 0.091
0.842MetPhe: 0.842 ± 0.046
1.538MetGly: 1.538 ± 0.071
0.441MetHis: 0.441 ± 0.036
1.834MetIle: 1.834 ± 0.076
2.155MetLys: 2.155 ± 0.079
2.14MetLeu: 2.14 ± 0.095
0.768MetMet: 0.768 ± 0.047
1.338MetAsn: 1.338 ± 0.065
1.104MetPro: 1.104 ± 0.056
0.866MetGln: 0.866 ± 0.049
1.28MetArg: 1.28 ± 0.067
1.631MetSer: 1.631 ± 0.07
1.569MetThr: 1.569 ± 0.07
1.717MetVal: 1.717 ± 0.073
0.102MetTrp: 0.102 ± 0.017
0.78MetTyr: 0.78 ± 0.049
0.028MetXaa: 0.028 ± 0.009
Asn
2.504AsnAla: 2.504 ± 0.087
0.355AsnCys: 0.355 ± 0.033
2.377AsnAsp: 2.377 ± 0.091
3.539AsnGlu: 3.539 ± 0.105
2.084AsnPhe: 2.084 ± 0.084
2.642AsnGly: 2.642 ± 0.103
0.734AsnHis: 0.734 ± 0.055
3.358AsnIle: 3.358 ± 0.099
2.146AsnLys: 2.146 ± 0.091
4.085AsnLeu: 4.085 ± 0.117
1.165AsnMet: 1.165 ± 0.061
1.831AsnAsn: 1.831 ± 0.098
2.374AsnPro: 2.374 ± 0.089
1.887AsnGln: 1.887 ± 0.083
2.254AsnArg: 2.254 ± 0.084
3.617AsnSer: 3.617 ± 0.115
2.325AsnThr: 2.325 ± 0.08
2.707AsnVal: 2.707 ± 0.101
0.416AsnTrp: 0.416 ± 0.038
1.797AsnTyr: 1.797 ± 0.076
0.034AsnXaa: 0.034 ± 0.01
Pro
1.871ProAla: 1.871 ± 0.077
0.216ProCys: 0.216 ± 0.022
2.534ProAsp: 2.534 ± 0.086
4.742ProGlu: 4.742 ± 0.138
1.239ProPhe: 1.239 ± 0.06
2.608ProGly: 2.608 ± 0.081
0.601ProHis: 0.601 ± 0.046
1.754ProIle: 1.754 ± 0.079
1.754ProLys: 1.754 ± 0.077
2.257ProLeu: 2.257 ± 0.091
0.669ProMet: 0.669 ± 0.045
1.409ProAsn: 1.409 ± 0.069
1.079ProPro: 1.079 ± 0.055
1.014ProGln: 1.014 ± 0.063
1.354ProArg: 1.354 ± 0.054
2.433ProSer: 2.433 ± 0.091
1.437ProThr: 1.437 ± 0.068
2.642ProVal: 2.642 ± 0.094
0.318ProTrp: 0.318 ± 0.034
1.088ProTyr: 1.088 ± 0.06
0.015ProXaa: 0.015 ± 0.007
Gln
2.278GlnAla: 2.278 ± 0.093
0.222GlnCys: 0.222 ± 0.024
2.624GlnAsp: 2.624 ± 0.095
3.472GlnGlu: 3.472 ± 0.114
1.005GlnPhe: 1.005 ± 0.053
2.158GlnGly: 2.158 ± 0.084
0.518GlnHis: 0.518 ± 0.041
2.257GlnIle: 2.257 ± 0.096
2.51GlnLys: 2.51 ± 0.104
2.676GlnLeu: 2.676 ± 0.102
1.017GlnMet: 1.017 ± 0.065
1.875GlnAsn: 1.875 ± 0.073
0.823GlnPro: 0.823 ± 0.052
1.538GlnGln: 1.538 ± 0.102
1.616GlnArg: 1.616 ± 0.074
2.093GlnSer: 2.093 ± 0.091
1.597GlnThr: 1.597 ± 0.074
2.084GlnVal: 2.084 ± 0.089
0.163GlnTrp: 0.163 ± 0.022
0.891GlnTyr: 0.891 ± 0.057
0.015GlnXaa: 0.015 ± 0.006
Arg
2.473ArgAla: 2.473 ± 0.104
0.373ArgCys: 0.373 ± 0.033
3.444ArgAsp: 3.444 ± 0.109
5.917ArgGlu: 5.917 ± 0.147
1.637ArgPhe: 1.637 ± 0.062
3.151ArgGly: 3.151 ± 0.096
0.641ArgHis: 0.641 ± 0.037
3.354ArgIle: 3.354 ± 0.099
3.885ArgLys: 3.885 ± 0.138
3.398ArgLeu: 3.398 ± 0.11
1.511ArgMet: 1.511 ± 0.066
2.399ArgAsn: 2.399 ± 0.102
2.497ArgPro: 2.497 ± 0.085
1.532ArgGln: 1.532 ± 0.069
2.522ArgArg: 2.522 ± 0.098
2.833ArgSer: 2.833 ± 0.098
2.201ArgThr: 2.201 ± 0.089
3.049ArgVal: 3.049 ± 0.1
0.407ArgTrp: 0.407 ± 0.037
1.428ArgTyr: 1.428 ± 0.067
0.037ArgXaa: 0.037 ± 0.01
Ser
3.7SerAla: 3.7 ± 0.123
0.459SerCys: 0.459 ± 0.043
4.945SerAsp: 4.945 ± 0.148
7.418SerGlu: 7.418 ± 0.177
3.194SerPhe: 3.194 ± 0.109
5.904SerGly: 5.904 ± 0.159
1.098SerHis: 1.098 ± 0.061
4.261SerIle: 4.261 ± 0.129
4.082SerLys: 4.082 ± 0.138
5.661SerLeu: 5.661 ± 0.154
1.85SerMet: 1.85 ± 0.091
2.944SerAsn: 2.944 ± 0.103
2.377SerPro: 2.377 ± 0.078
2.716SerGln: 2.716 ± 0.096
3.765SerArg: 3.765 ± 0.106
5.833SerSer: 5.833 ± 0.176
3.623SerThr: 3.623 ± 0.15
4.76SerVal: 4.76 ± 0.12
0.617SerTrp: 0.617 ± 0.045
2.078SerTyr: 2.078 ± 0.078
0.037SerXaa: 0.037 ± 0.011
Thr
3.268ThrAla: 3.268 ± 0.123
0.265ThrCys: 0.265 ± 0.028
3.496ThrAsp: 3.496 ± 0.104
4.807ThrGlu: 4.807 ± 0.135
1.788ThrPhe: 1.788 ± 0.076
4.662ThrGly: 4.662 ± 0.113
0.848ThrHis: 0.848 ± 0.054
2.759ThrIle: 2.759 ± 0.099
1.834ThrLys: 1.834 ± 0.078
3.999ThrLeu: 3.999 ± 0.105
0.95ThrMet: 0.95 ± 0.057
1.542ThrAsn: 1.542 ± 0.076
1.912ThrPro: 1.912 ± 0.082
1.825ThrGln: 1.825 ± 0.073
2.26ThrArg: 2.26 ± 0.082
3.706ThrSer: 3.706 ± 0.122
2.423ThrThr: 2.423 ± 0.094
4.255ThrVal: 4.255 ± 0.141
0.422ThrTrp: 0.422 ± 0.031
1.511ThrTyr: 1.511 ± 0.072
0.028ThrXaa: 0.028 ± 0.01
Val
3.546ValAla: 3.546 ± 0.112
0.376ValCys: 0.376 ± 0.033
4.467ValAsp: 4.467 ± 0.144
8.442ValGlu: 8.442 ± 0.174
2.87ValPhe: 2.87 ± 0.094
3.922ValGly: 3.922 ± 0.125
1.002ValHis: 1.002 ± 0.057
4.187ValIle: 4.187 ± 0.131
4.39ValLys: 4.39 ± 0.134
5.775ValLeu: 5.775 ± 0.174
1.646ValMet: 1.646 ± 0.065
2.84ValAsn: 2.84 ± 0.096
2.192ValPro: 2.192 ± 0.09
2.016ValGln: 2.016 ± 0.077
3.132ValArg: 3.132 ± 0.106
4.523ValSer: 4.523 ± 0.121
3.539ValThr: 3.539 ± 0.114
4.495ValVal: 4.495 ± 0.146
0.503ValTrp: 0.503 ± 0.037
2.109ValTyr: 2.109 ± 0.083
0.018ValXaa: 0.018 ± 0.007
Trp
0.521TrpAla: 0.521 ± 0.046
0.052TrpCys: 0.052 ± 0.013
0.604TrpAsp: 0.604 ± 0.039
0.789TrpGlu: 0.789 ± 0.049
0.271TrpPhe: 0.271 ± 0.029
0.555TrpGly: 0.555 ± 0.046
0.154TrpHis: 0.154 ± 0.024
0.66TrpIle: 0.66 ± 0.049
0.734TrpLys: 0.734 ± 0.05
0.654TrpLeu: 0.654 ± 0.053
0.314TrpMet: 0.314 ± 0.038
0.53TrpAsn: 0.53 ± 0.046
0.2TrpPro: 0.2 ± 0.025
0.234TrpGln: 0.234 ± 0.028
0.601TrpArg: 0.601 ± 0.047
0.647TrpSer: 0.647 ± 0.047
0.395TrpThr: 0.395 ± 0.037
0.333TrpVal: 0.333 ± 0.032
0.077TrpTrp: 0.077 ± 0.014
0.311TrpTyr: 0.311 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.757TyrAla: 1.757 ± 0.076
0.392TyrCys: 0.392 ± 0.031
2.386TyrAsp: 2.386 ± 0.089
2.491TyrGlu: 2.491 ± 0.094
1.48TyrPhe: 1.48 ± 0.066
2.177TyrGly: 2.177 ± 0.081
0.472TyrHis: 0.472 ± 0.037
2.053TyrIle: 2.053 ± 0.078
1.384TyrLys: 1.384 ± 0.069
3.173TyrLeu: 3.173 ± 0.108
0.842TyrMet: 0.842 ± 0.052
1.474TyrAsn: 1.474 ± 0.077
1.258TyrPro: 1.258 ± 0.065
1.064TyrGln: 1.064 ± 0.057
2.029TyrArg: 2.029 ± 0.08
3.043TyrSer: 3.043 ± 0.099
1.831TyrThr: 1.831 ± 0.079
1.637TyrVal: 1.637 ± 0.076
0.438TyrTrp: 0.438 ± 0.034
1.008TyrTyr: 1.008 ± 0.053
0.015TyrXaa: 0.015 ± 0.007
Xaa
0.028XaaAla: 0.028 ± 0.009
0.003XaaCys: 0.003 ± 0.003
0.025XaaAsp: 0.025 ± 0.009
0.049XaaGlu: 0.049 ± 0.012
0.031XaaPhe: 0.031 ± 0.01
0.068XaaGly: 0.068 ± 0.012
0.003XaaHis: 0.003 ± 0.003
0.028XaaIle: 0.028 ± 0.008
0.055XaaLys: 0.055 ± 0.014
0.046XaaLeu: 0.046 ± 0.012
0.028XaaMet: 0.028 ± 0.009
0.028XaaAsn: 0.028 ± 0.009
0.012XaaPro: 0.012 ± 0.006
0.022XaaGln: 0.022 ± 0.008
0.04XaaArg: 0.04 ± 0.012
0.04XaaSer: 0.04 ± 0.01
0.028XaaThr: 0.028 ± 0.01
0.015XaaVal: 0.015 ± 0.007
0.006XaaTrp: 0.006 ± 0.005
0.015XaaTyr: 0.015 ± 0.007
0.219XaaXaa: 0.219 ± 0.04
Statistics based on 2152 proteins (324344 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski