Mycoplasma suis (strain Illinois)
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F0QS33|F0QS33_MYCSL Uncharacterized protein OS=Mycoplasma suis (strain Illinois) OX=768700 GN=MSU_0778 PE=4 SV=1
MM1 pKa = 7.39 EE2 pKa = 6.16 AGFAGDD8 pKa = 3.25 WTGCEE13 pKa = 4.2 ILWLTASLLTLWEE26 pKa = 4.67 SEE28 pKa = 4.39 FLWVSSVFVNFSSFLTSLTTCGLLFFEE55 pKa = 4.59 SWLWTDD61 pKa = 3.27 EE62 pKa = 4.3 AGEE65 pKa = 4.24 SFAWTEE71 pKa = 4.48 SPCGLSDD78 pKa = 3.75 SFLINSLCDD87 pKa = 3.08 WLLPSNEE94 pKa = 4.42 PKK96 pKa = 10.11 PEE98 pKa = 4.1 PMPPPRR104 pKa = 11.84 NLRR107 pKa = 11.84 KK108 pKa = 10.04 LIPLILL114 pKa = 4.75
Molecular weight: 12.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.099
IPC2_protein 3.999
IPC_protein 3.846
Toseland 3.694
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.694
Grimsley 3.617
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.024
Thurlkill 3.732
EMBOSS 3.706
Sillero 3.961
Patrickios 3.058
IPC_peptide 3.783
IPC2_peptide 3.948
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|F0QR51|F0QR51_MYCSL 50S ribosomal protein L18 OS=Mycoplasma suis (strain Illinois) OX=768700 GN=rplR PE=4 SV=1
MM1 pKa = 7.85 AIKK4 pKa = 10.36 QKK6 pKa = 10.2 VPRR9 pKa = 11.84 SSGRR13 pKa = 11.84 RR14 pKa = 11.84 VVISIDD20 pKa = 3.46 YY21 pKa = 10.88 NKK23 pKa = 9.66 TLTKK27 pKa = 10.5 DD28 pKa = 3.46 RR29 pKa = 11.84 PHH31 pKa = 6.84 KK32 pKa = 10.37 PLLTKK37 pKa = 10.54 LKK39 pKa = 10.38 KK40 pKa = 9.72 HH41 pKa = 6.34 AGRR44 pKa = 11.84 NNTGQITVRR53 pKa = 11.84 HH54 pKa = 5.99 RR55 pKa = 11.84 GGEE58 pKa = 3.95 NKK60 pKa = 9.74 RR61 pKa = 11.84 KK62 pKa = 9.89 YY63 pKa = 10.46 RR64 pKa = 11.84 IIDD67 pKa = 3.8 FKK69 pKa = 10.99 RR70 pKa = 11.84 DD71 pKa = 3.21 KK72 pKa = 11.29 LNVKK76 pKa = 10.03 GVVQTIEE83 pKa = 3.83 YY84 pKa = 10.1 DD85 pKa = 3.5 PNRR88 pKa = 11.84 TCFISLVHH96 pKa = 5.98 YY97 pKa = 10.95 ADD99 pKa = 3.63 GEE101 pKa = 4.12 KK102 pKa = 10.17 RR103 pKa = 11.84 YY104 pKa = 9.97 ILAPSEE110 pKa = 4.14 IKK112 pKa = 10.64 KK113 pKa = 10.8 GDD115 pKa = 3.41 IVVSSMEE122 pKa = 4.06 RR123 pKa = 11.84 TNLRR127 pKa = 11.84 IGDD130 pKa = 4.1 CMPIGVIPEE139 pKa = 3.56 GMMVHH144 pKa = 6.73 NIEE147 pKa = 4.64 IKK149 pKa = 10.53 PGDD152 pKa = 3.78 GGKK155 pKa = 8.64 MVRR158 pKa = 11.84 SAGSQAQILGKK169 pKa = 10.15 DD170 pKa = 3.32 LTGKK174 pKa = 8.41 YY175 pKa = 8.9 TIIKK179 pKa = 9.66 LPSKK183 pKa = 9.15 EE184 pKa = 3.71 VRR186 pKa = 11.84 KK187 pKa = 10.26 VINGARR193 pKa = 11.84 ATIGQLSAIEE203 pKa = 4.31 HH204 pKa = 5.65 NLVRR208 pKa = 11.84 LGTAGKK214 pKa = 9.46 NRR216 pKa = 11.84 HH217 pKa = 5.69 LGIRR221 pKa = 11.84 PTVRR225 pKa = 11.84 GVAMNPNDD233 pKa = 4.55 HH234 pKa = 6.27 IHH236 pKa = 6.44 GGGEE240 pKa = 4.26 GRR242 pKa = 11.84 CSIGRR247 pKa = 11.84 DD248 pKa = 3.47 APRR251 pKa = 11.84 TPWGKK256 pKa = 9.41 RR257 pKa = 11.84 HH258 pKa = 6.37 LGVKK262 pKa = 8.64 TRR264 pKa = 11.84 SKK266 pKa = 10.6 KK267 pKa = 9.81 RR268 pKa = 11.84 YY269 pKa = 7.02 SNRR272 pKa = 11.84 MIVQRR277 pKa = 11.84 RR278 pKa = 11.84 NAKK281 pKa = 9.37 NN282 pKa = 3.0
Molecular weight: 31.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.882
IPC_protein 10.672
Toseland 11.052
ProMoST 10.774
Dawson 11.111
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.403
Grimsley 11.14
Solomon 11.272
Lehninger 11.242
Nozaki 11.023
DTASelect 10.818
Thurlkill 11.038
EMBOSS 11.462
Sillero 11.038
Patrickios 11.111
IPC_peptide 11.286
IPC2_peptide 9.706
IPC2.peptide.svr19 8.542
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
844
0
844
219786
33
2643
260.4
29.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.647 ± 0.074
1.316 ± 0.038
4.465 ± 0.067
9.19 ± 0.101
5.234 ± 0.096
6.018 ± 0.097
1.281 ± 0.033
7.075 ± 0.089
10.23 ± 0.093
9.722 ± 0.102
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.536 ± 0.025
6.056 ± 0.067
2.767 ± 0.043
3.701 ± 0.062
3.723 ± 0.054
9.659 ± 0.106
4.468 ± 0.056
5.084 ± 0.068
1.703 ± 0.043
3.126 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here