Mycoplasma suis (strain Illinois)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma suis

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F0QS33|F0QS33_MYCSL Uncharacterized protein OS=Mycoplasma suis (strain Illinois) OX=768700 GN=MSU_0778 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 6.16AGFAGDD8 pKa = 3.25WTGCEE13 pKa = 4.2ILWLTASLLTLWEE26 pKa = 4.67SEE28 pKa = 4.39FLWVSSVFVNFSSFLTSLTTCGLLFFEE55 pKa = 4.59SWLWTDD61 pKa = 3.27EE62 pKa = 4.3AGEE65 pKa = 4.24SFAWTEE71 pKa = 4.48SPCGLSDD78 pKa = 3.75SFLINSLCDD87 pKa = 3.08WLLPSNEE94 pKa = 4.42PKK96 pKa = 10.11PEE98 pKa = 4.1PMPPPRR104 pKa = 11.84NLRR107 pKa = 11.84KK108 pKa = 10.04LIPLILL114 pKa = 4.75

Molecular weight:
12.9 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F0QR51|F0QR51_MYCSL 50S ribosomal protein L18 OS=Mycoplasma suis (strain Illinois) OX=768700 GN=rplR PE=4 SV=1
MM1 pKa = 7.85AIKK4 pKa = 10.36QKK6 pKa = 10.2VPRR9 pKa = 11.84SSGRR13 pKa = 11.84RR14 pKa = 11.84VVISIDD20 pKa = 3.46YY21 pKa = 10.88NKK23 pKa = 9.66TLTKK27 pKa = 10.5DD28 pKa = 3.46RR29 pKa = 11.84PHH31 pKa = 6.84KK32 pKa = 10.37PLLTKK37 pKa = 10.54LKK39 pKa = 10.38KK40 pKa = 9.72HH41 pKa = 6.34AGRR44 pKa = 11.84NNTGQITVRR53 pKa = 11.84HH54 pKa = 5.99RR55 pKa = 11.84GGEE58 pKa = 3.95NKK60 pKa = 9.74RR61 pKa = 11.84KK62 pKa = 9.89YY63 pKa = 10.46RR64 pKa = 11.84IIDD67 pKa = 3.8FKK69 pKa = 10.99RR70 pKa = 11.84DD71 pKa = 3.21KK72 pKa = 11.29LNVKK76 pKa = 10.03GVVQTIEE83 pKa = 3.83YY84 pKa = 10.1DD85 pKa = 3.5PNRR88 pKa = 11.84TCFISLVHH96 pKa = 5.98YY97 pKa = 10.95ADD99 pKa = 3.63GEE101 pKa = 4.12KK102 pKa = 10.17RR103 pKa = 11.84YY104 pKa = 9.97ILAPSEE110 pKa = 4.14IKK112 pKa = 10.64KK113 pKa = 10.8GDD115 pKa = 3.41IVVSSMEE122 pKa = 4.06RR123 pKa = 11.84TNLRR127 pKa = 11.84IGDD130 pKa = 4.1CMPIGVIPEE139 pKa = 3.56GMMVHH144 pKa = 6.73NIEE147 pKa = 4.64IKK149 pKa = 10.53PGDD152 pKa = 3.78GGKK155 pKa = 8.64MVRR158 pKa = 11.84SAGSQAQILGKK169 pKa = 10.15DD170 pKa = 3.32LTGKK174 pKa = 8.41YY175 pKa = 8.9TIIKK179 pKa = 9.66LPSKK183 pKa = 9.15EE184 pKa = 3.71VRR186 pKa = 11.84KK187 pKa = 10.26VINGARR193 pKa = 11.84ATIGQLSAIEE203 pKa = 4.31HH204 pKa = 5.65NLVRR208 pKa = 11.84LGTAGKK214 pKa = 9.46NRR216 pKa = 11.84HH217 pKa = 5.69LGIRR221 pKa = 11.84PTVRR225 pKa = 11.84GVAMNPNDD233 pKa = 4.55HH234 pKa = 6.27IHH236 pKa = 6.44GGGEE240 pKa = 4.26GRR242 pKa = 11.84CSIGRR247 pKa = 11.84DD248 pKa = 3.47APRR251 pKa = 11.84TPWGKK256 pKa = 9.41RR257 pKa = 11.84HH258 pKa = 6.37LGVKK262 pKa = 8.64TRR264 pKa = 11.84SKK266 pKa = 10.6KK267 pKa = 9.81RR268 pKa = 11.84YY269 pKa = 7.02SNRR272 pKa = 11.84MIVQRR277 pKa = 11.84RR278 pKa = 11.84NAKK281 pKa = 9.37NN282 pKa = 3.0

Molecular weight:
31.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

844

0

844

219786

33

2643

260.4

29.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.647 ± 0.074

1.316 ± 0.038

4.465 ± 0.067

9.19 ± 0.101

5.234 ± 0.096

6.018 ± 0.097

1.281 ± 0.033

7.075 ± 0.089

10.23 ± 0.093

9.722 ± 0.102

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.536 ± 0.025

6.056 ± 0.067

2.767 ± 0.043

3.701 ± 0.062

3.723 ± 0.054

9.659 ± 0.106

4.468 ± 0.056

5.084 ± 0.068

1.703 ± 0.043

3.126 ± 0.055

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski