Streptomyces sp. NRRL S-495

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4J6J6|A0A0F4J6J6_9ACTN Endoribonuclease L-PSP OS=Streptomyces sp. NRRL S-495 OX=1609133 GN=VR45_28840 PE=4 SV=1
MM1 pKa = 7.78PEE3 pKa = 3.59VTTPYY8 pKa = 8.89PTGTPCWVDD17 pKa = 5.27LMAKK21 pKa = 9.66DD22 pKa = 3.44QQAALDD28 pKa = 4.19FYY30 pKa = 11.32RR31 pKa = 11.84DD32 pKa = 3.4LFGWQGAPGPAEE44 pKa = 3.74FGGYY48 pKa = 8.87AVCEE52 pKa = 4.09LNGRR56 pKa = 11.84AVAGIGPAMSPDD68 pKa = 3.75GAPEE72 pKa = 4.27PPTVWTGYY80 pKa = 9.67LASTDD85 pKa = 3.49AQATQDD91 pKa = 4.1AIVAAGGTLLAPVMDD106 pKa = 4.24VGRR109 pKa = 11.84LGRR112 pKa = 11.84MLVAADD118 pKa = 3.62PQGAAFF124 pKa = 4.47

Molecular weight: 12.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4JYW3|A0A0F4JYW3_9ACTN IclR family transcriptional regulator OS=Streptomyces sp. NRRL S-495 OX=1609133 GN=VR45_02430 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.77GRR40 pKa = 11.84TSLSAA45 pKa = 3.59

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7273

0

7273

2134324

26

2619

293.5

31.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.308 ± 0.05

0.753 ± 0.007

5.826 ± 0.022

5.575 ± 0.031

2.705 ± 0.018

9.788 ± 0.03

2.233 ± 0.014

2.88 ± 0.022

1.798 ± 0.026

10.84 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.538 ± 0.011

1.735 ± 0.019

6.386 ± 0.03

2.668 ± 0.017

8.093 ± 0.037

4.734 ± 0.023

6.15 ± 0.026

8.496 ± 0.025

1.52 ± 0.013

1.973 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski