Amino acid dipepetide frequency for Streptomyces sp. NRRL S-495

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.426AlaAla: 24.426 ± 0.179	1.001AlaCys: 1.001 ± 0.025	8.857AlaAsp: 8.857 ± 0.078	9.645AlaGlu: 9.645 ± 0.104	3.677AlaPhe: 3.677 ± 0.049	14.58AlaGly: 14.58 ± 0.094	2.814AlaHis: 2.814 ± 0.042	3.212AlaIle: 3.212 ± 0.041	2.552AlaLys: 2.552 ± 0.052	15.398AlaLeu: 15.398 ± 0.123	2.336AlaMet: 2.336 ± 0.035	2.005AlaAsn: 2.005 ± 0.035	7.974AlaPro: 7.974 ± 0.083	3.533AlaGln: 3.533 ± 0.049	10.46AlaArg: 10.46 ± 0.081	5.377AlaSer: 5.377 ± 0.06	7.485AlaThr: 7.485 ± 0.064	13.382AlaVal: 13.382 ± 0.108	1.907AlaTrp: 1.907 ± 0.031	2.459AlaTyr: 2.459 ± 0.035	0.001AlaXaa: 0.001 ± 0.001
Cys	1.036CysAla: 1.036 ± 0.023	0.091CysCys: 0.091 ± 0.007	0.412CysAsp: 0.412 ± 0.014	0.372CysGlu: 0.372 ± 0.012	0.221CysPhe: 0.221 ± 0.011	0.897CysGly: 0.897 ± 0.021	0.193CysHis: 0.193 ± 0.01	0.151CysIle: 0.151 ± 0.008	0.09CysLys: 0.09 ± 0.007	0.789CysLeu: 0.789 ± 0.02	0.103CysMet: 0.103 ± 0.007	0.134CysAsn: 0.134 ± 0.008	0.494CysPro: 0.494 ± 0.016	0.168CysGln: 0.168 ± 0.009	0.586CysArg: 0.586 ± 0.018	0.437CysSer: 0.437 ± 0.016	0.506CysThr: 0.506 ± 0.016	0.563CysVal: 0.563 ± 0.015	0.124CysTrp: 0.124 ± 0.008	0.158CysTyr: 0.158 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.378AspAla: 7.378 ± 0.061	0.44AspCys: 0.44 ± 0.016	2.955AspAsp: 2.955 ± 0.037	3.508AspGlu: 3.508 ± 0.047	1.626AspPhe: 1.626 ± 0.032	6.733AspGly: 6.733 ± 0.069	1.426AspHis: 1.426 ± 0.027	1.68AspIle: 1.68 ± 0.031	0.95AspLys: 0.95 ± 0.026	6.514AspLeu: 6.514 ± 0.067	0.636AspMet: 0.636 ± 0.019	0.945AspAsn: 0.945 ± 0.025	4.802AspPro: 4.802 ± 0.059	1.504AspGln: 1.504 ± 0.027	5.397AspArg: 5.397 ± 0.052	2.333AspSer: 2.333 ± 0.038	3.156AspThr: 3.156 ± 0.04	4.057AspVal: 4.057 ± 0.043	1.059AspTrp: 1.059 ± 0.023	1.156AspTyr: 1.156 ± 0.021	0.001AspXaa: 0.001 ± 0.001
Glu	7.352GluAla: 7.352 ± 0.074	0.339GluCys: 0.339 ± 0.012	2.745GluAsp: 2.745 ± 0.036	3.422GluGlu: 3.422 ± 0.05	1.495GluPhe: 1.495 ± 0.028	4.124GluGly: 4.124 ± 0.049	1.563GluHis: 1.563 ± 0.03	1.975GluIle: 1.975 ± 0.03	1.115GluLys: 1.115 ± 0.03	7.421GluLeu: 7.421 ± 0.082	0.718GluMet: 0.718 ± 0.02	0.949GluAsn: 0.949 ± 0.025	3.4GluPro: 3.4 ± 0.044	2.358GluGln: 2.358 ± 0.035	5.432GluArg: 5.432 ± 0.055	2.358GluSer: 2.358 ± 0.036	2.499GluThr: 2.499 ± 0.039	4.671GluVal: 4.671 ± 0.055	0.732GluTrp: 0.732 ± 0.016	1.087GluTyr: 1.087 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.784PheAla: 3.784 ± 0.049	0.261PheCys: 0.261 ± 0.01	1.98PheAsp: 1.98 ± 0.033	1.435PheGlu: 1.435 ± 0.027	0.8PhePhe: 0.8 ± 0.023	3.072PheGly: 3.072 ± 0.042	0.618PheHis: 0.618 ± 0.016	0.606PheIle: 0.606 ± 0.019	0.447PheLys: 0.447 ± 0.016	2.625PheLeu: 2.625 ± 0.04	0.354PheMet: 0.354 ± 0.012	0.543PheAsn: 0.543 ± 0.018	1.448PhePro: 1.448 ± 0.028	0.693PheGln: 0.693 ± 0.018	1.875PheArg: 1.875 ± 0.033	1.359PheSer: 1.359 ± 0.026	1.992PheThr: 1.992 ± 0.03	2.176PheVal: 2.176 ± 0.034	0.422PheTrp: 0.422 ± 0.014	0.561PheTyr: 0.561 ± 0.016	0.0PheXaa: 0.0 ± 0.001
Gly	12.073GlyAla: 12.073 ± 0.078	0.813GlyCys: 0.813 ± 0.021	4.854GlyAsp: 4.854 ± 0.044	5.136GlyGlu: 5.136 ± 0.052	2.895GlyPhe: 2.895 ± 0.037	9.417GlyGly: 9.417 ± 0.095	2.379GlyHis: 2.379 ± 0.032	3.278GlyIle: 3.278 ± 0.044	2.085GlyLys: 2.085 ± 0.044	10.422GlyLeu: 10.422 ± 0.087	1.825GlyMet: 1.825 ± 0.03	1.771GlyAsn: 1.771 ± 0.038	6.091GlyPro: 6.091 ± 0.063	2.67GlyGln: 2.67 ± 0.034	8.481GlyArg: 8.481 ± 0.082	5.446GlySer: 5.446 ± 0.057	6.671GlyThr: 6.671 ± 0.065	7.467GlyVal: 7.467 ± 0.063	1.8GlyTrp: 1.8 ± 0.03	2.307GlyTyr: 2.307 ± 0.034	0.001GlyXaa: 0.001 ± 0.001
His	2.566HisAla: 2.566 ± 0.036	0.229HisCys: 0.229 ± 0.012	1.224HisAsp: 1.224 ± 0.027	1.124HisGlu: 1.124 ± 0.023	0.589HisPhe: 0.589 ± 0.016	2.409HisGly: 2.409 ± 0.032	0.721HisHis: 0.721 ± 0.022	0.581HisIle: 0.581 ± 0.016	0.269HisLys: 0.269 ± 0.012	2.604HisLeu: 2.604 ± 0.039	0.284HisMet: 0.284 ± 0.013	0.376HisAsn: 0.376 ± 0.014	1.88HisPro: 1.88 ± 0.031	0.657HisGln: 0.657 ± 0.018	2.302HisArg: 2.302 ± 0.041	0.951HisSer: 0.951 ± 0.021	1.205HisThr: 1.205 ± 0.021	1.467HisVal: 1.467 ± 0.028	0.416HisTrp: 0.416 ± 0.013	0.47HisTyr: 0.47 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.503IleAla: 4.503 ± 0.049	0.278IleCys: 0.278 ± 0.012	2.048IleAsp: 2.048 ± 0.037	1.785IleGlu: 1.785 ± 0.032	0.589IlePhe: 0.589 ± 0.018	3.577IleGly: 3.577 ± 0.041	0.569IleHis: 0.569 ± 0.017	0.674IleIle: 0.674 ± 0.022	0.64IleLys: 0.64 ± 0.02	2.128IleLeu: 2.128 ± 0.038	0.378IleMet: 0.378 ± 0.014	0.628IleAsn: 0.628 ± 0.019	1.547IlePro: 1.547 ± 0.032	0.63IleGln: 0.63 ± 0.017	2.028IleArg: 2.028 ± 0.03	1.489IleSer: 1.489 ± 0.032	2.098IleThr: 2.098 ± 0.033	2.404IleVal: 2.404 ± 0.039	0.361IleTrp: 0.361 ± 0.013	0.445IleTyr: 0.445 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.528LysAla: 2.528 ± 0.05	0.091LysCys: 0.091 ± 0.007	1.124LysAsp: 1.124 ± 0.031	0.931LysGlu: 0.931 ± 0.027	0.397LysPhe: 0.397 ± 0.013	1.502LysGly: 1.502 ± 0.034	0.411LysHis: 0.411 ± 0.012	0.67LysIle: 0.67 ± 0.02	0.569LysLys: 0.569 ± 0.025	1.787LysLeu: 1.787 ± 0.036	0.305LysMet: 0.305 ± 0.011	0.447LysAsn: 0.447 ± 0.019	1.116LysPro: 1.116 ± 0.028	0.64LysGln: 0.64 ± 0.018	1.162LysArg: 1.162 ± 0.027	0.951LysSer: 0.951 ± 0.025	0.997LysThr: 0.997 ± 0.027	1.701LysVal: 1.701 ± 0.035	0.224LysTrp: 0.224 ± 0.012	0.421LysTyr: 0.421 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	17.132LeuAla: 17.132 ± 0.142	0.843LeuCys: 0.843 ± 0.021	7.228LeuAsp: 7.228 ± 0.065	4.658LeuGlu: 4.658 ± 0.048	2.67LeuPhe: 2.67 ± 0.041	10.08LeuGly: 10.08 ± 0.078	2.369LeuHis: 2.369 ± 0.039	2.929LeuIle: 2.929 ± 0.039	1.725LeuLys: 1.725 ± 0.035	12.54LeuLeu: 12.54 ± 0.124	1.521LeuMet: 1.521 ± 0.027	1.69LeuAsn: 1.69 ± 0.03	7.129LeuPro: 7.129 ± 0.066	2.134LeuGln: 2.134 ± 0.032	9.082LeuArg: 9.082 ± 0.079	5.02LeuSer: 5.02 ± 0.052	7.13LeuThr: 7.13 ± 0.062	9.446LeuVal: 9.446 ± 0.082	1.315LeuTrp: 1.315 ± 0.026	1.764LeuTyr: 1.764 ± 0.032	0.001LeuXaa: 0.001 ± 0.001
Met	2.068MetAla: 2.068 ± 0.033	0.11MetCys: 0.11 ± 0.008	0.789MetAsp: 0.789 ± 0.019	0.611MetGlu: 0.611 ± 0.018	0.445MetPhe: 0.445 ± 0.016	1.143MetGly: 1.143 ± 0.028	0.295MetHis: 0.295 ± 0.011	0.595MetIle: 0.595 ± 0.018	0.338MetLys: 0.338 ± 0.014	1.596MetLeu: 1.596 ± 0.032	0.259MetMet: 0.259 ± 0.011	0.367MetAsn: 0.367 ± 0.015	1.027MetPro: 1.027 ± 0.022	0.371MetGln: 0.371 ± 0.014	1.214MetArg: 1.214 ± 0.025	1.128MetSer: 1.128 ± 0.022	1.366MetThr: 1.366 ± 0.024	1.221MetVal: 1.221 ± 0.028	0.169MetTrp: 0.169 ± 0.01	0.272MetTyr: 0.272 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.173AsnAla: 2.173 ± 0.04	0.156AsnCys: 0.156 ± 0.008	0.898AsnAsp: 0.898 ± 0.02	0.756AsnGlu: 0.756 ± 0.021	0.461AsnPhe: 0.461 ± 0.016	1.99AsnGly: 1.99 ± 0.041	0.388AsnHis: 0.388 ± 0.012	0.594AsnIle: 0.594 ± 0.017	0.348AsnLys: 0.348 ± 0.015	1.711AsnLeu: 1.711 ± 0.03	0.253AsnMet: 0.253 ± 0.012	0.427AsnAsn: 0.427 ± 0.015	1.41AsnPro: 1.41 ± 0.026	0.512AsnGln: 0.512 ± 0.018	1.291AsnArg: 1.291 ± 0.025	0.923AsnSer: 0.923 ± 0.023	1.127AsnThr: 1.127 ± 0.029	1.251AsnVal: 1.251 ± 0.023	0.283AsnTrp: 0.283 ± 0.012	0.403AsnTyr: 0.403 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	10.281ProAla: 10.281 ± 0.095	0.327ProCys: 0.327 ± 0.012	4.649ProAsp: 4.649 ± 0.06	4.422ProGlu: 4.422 ± 0.047	1.586ProPhe: 1.586 ± 0.028	7.482ProGly: 7.482 ± 0.063	1.249ProHis: 1.249 ± 0.028	1.331ProIle: 1.331 ± 0.023	1.035ProLys: 1.035 ± 0.024	5.578ProLeu: 5.578 ± 0.058	0.908ProMet: 0.908 ± 0.021	0.978ProAsn: 0.978 ± 0.022	3.572ProPro: 3.572 ± 0.053	1.707ProGln: 1.707 ± 0.032	3.852ProArg: 3.852 ± 0.043	3.063ProSer: 3.063 ± 0.044	3.648ProThr: 3.648 ± 0.056	5.98ProVal: 5.98 ± 0.05	0.919ProTrp: 0.919 ± 0.022	1.298ProTyr: 1.298 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.625GlnAla: 3.625 ± 0.047	0.175GlnCys: 0.175 ± 0.009	1.429GlnAsp: 1.429 ± 0.026	1.358GlnGlu: 1.358 ± 0.027	0.658GlnPhe: 0.658 ± 0.017	2.168GlnGly: 2.168 ± 0.032	0.625GlnHis: 0.625 ± 0.021	0.963GlnIle: 0.963 ± 0.022	0.516GlnLys: 0.516 ± 0.019	3.194GlnLeu: 3.194 ± 0.04	0.38GlnMet: 0.38 ± 0.014	0.491GlnAsn: 0.491 ± 0.016	1.658GlnPro: 1.658 ± 0.033	1.3GlnGln: 1.3 ± 0.044	2.283GlnArg: 2.283 ± 0.037	1.21GlnSer: 1.21 ± 0.023	1.176GlnThr: 1.176 ± 0.025	2.379GlnVal: 2.379 ± 0.032	0.477GlnTrp: 0.477 ± 0.015	0.619GlnTyr: 0.619 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	10.491ArgAla: 10.491 ± 0.096	0.541ArgCys: 0.541 ± 0.016	3.914ArgAsp: 3.914 ± 0.046	4.686ArgGlu: 4.686 ± 0.052	2.361ArgPhe: 2.361 ± 0.036	5.756ArgGly: 5.756 ± 0.056	2.04ArgHis: 2.04 ± 0.037	3.357ArgIle: 3.357 ± 0.049	1.318ArgLys: 1.318 ± 0.032	9.414ArgLeu: 9.414 ± 0.084	1.63ArgMet: 1.63 ± 0.028	1.318ArgAsn: 1.318 ± 0.026	5.61ArgPro: 5.61 ± 0.068	2.185ArgGln: 2.185 ± 0.033	7.903ArgArg: 7.903 ± 0.082	4.077ArgSer: 4.077 ± 0.05	5.536ArgThr: 5.536 ± 0.05	5.648ArgVal: 5.648 ± 0.051	1.376ArgTrp: 1.376 ± 0.027	1.765ArgTyr: 1.765 ± 0.03	0.001ArgXaa: 0.001 ± 0.001
Ser	6.602SerAla: 6.602 ± 0.057	0.43SerCys: 0.43 ± 0.014	2.31SerAsp: 2.31 ± 0.036	2.09SerGlu: 2.09 ± 0.032	1.497SerPhe: 1.497 ± 0.027	5.675SerGly: 5.675 ± 0.057	0.877SerHis: 0.877 ± 0.017	1.343SerIle: 1.343 ± 0.028	0.825SerLys: 0.825 ± 0.025	4.485SerLeu: 4.485 ± 0.048	0.934SerMet: 0.934 ± 0.022	0.855SerAsn: 0.855 ± 0.018	3.042SerPro: 3.042 ± 0.04	1.114SerGln: 1.114 ± 0.024	3.333SerArg: 3.333 ± 0.044	2.643SerSer: 2.643 ± 0.045	3.096SerThr: 3.096 ± 0.044	4.071SerVal: 4.071 ± 0.044	0.954SerTrp: 0.954 ± 0.024	1.163SerTyr: 1.163 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	9.811ThrAla: 9.811 ± 0.078	0.401ThrCys: 0.401 ± 0.014	3.605ThrAsp: 3.605 ± 0.043	3.168ThrGlu: 3.168 ± 0.039	1.572ThrPhe: 1.572 ± 0.032	7.398ThrGly: 7.398 ± 0.075	1.056ThrHis: 1.056 ± 0.022	1.41ThrIle: 1.41 ± 0.029	0.96ThrLys: 0.96 ± 0.024	5.739ThrLeu: 5.739 ± 0.054	0.837ThrMet: 0.837 ± 0.018	0.983ThrAsn: 0.983 ± 0.024	4.236ThrPro: 4.236 ± 0.056	1.14ThrGln: 1.14 ± 0.026	3.771ThrArg: 3.771 ± 0.041	2.799ThrSer: 2.799 ± 0.04	3.952ThrThr: 3.952 ± 0.061	6.658ThrVal: 6.658 ± 0.059	0.871ThrTrp: 0.871 ± 0.021	1.13ThrTyr: 1.13 ± 0.029	0.001ThrXaa: 0.001 ± 0.001
Val	11.061ValAla: 11.061 ± 0.084	0.682ValCys: 0.682 ± 0.017	5.152ValAsp: 5.152 ± 0.057	4.834ValGlu: 4.834 ± 0.054	2.409ValPhe: 2.409 ± 0.033	6.704ValGly: 6.704 ± 0.066	1.978ValHis: 1.978 ± 0.031	2.468ValIle: 2.468 ± 0.038	1.561ValLys: 1.561 ± 0.032	10.434ValLeu: 10.434 ± 0.086	1.256ValMet: 1.256 ± 0.031	1.653ValAsn: 1.653 ± 0.03	5.495ValPro: 5.495 ± 0.047	2.107ValGln: 2.107 ± 0.03	7.182ValArg: 7.182 ± 0.06	3.862ValSer: 3.862 ± 0.041	5.559ValThr: 5.559 ± 0.066	8.018ValVal: 8.018 ± 0.075	1.073ValTrp: 1.073 ± 0.024	1.469ValTyr: 1.469 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.731TrpAla: 1.731 ± 0.029	0.145TrpCys: 0.145 ± 0.007	0.804TrpAsp: 0.804 ± 0.022	0.749TrpGlu: 0.749 ± 0.02	0.504TrpPhe: 0.504 ± 0.016	1.032TrpGly: 1.032 ± 0.029	0.363TrpHis: 0.363 ± 0.014	0.541TrpIle: 0.541 ± 0.018	0.31TrpLys: 0.31 ± 0.014	1.783TrpLeu: 1.783 ± 0.032	0.25TrpMet: 0.25 ± 0.011	0.399TrpAsn: 0.399 ± 0.016	0.825TrpPro: 0.825 ± 0.023	0.631TrpGln: 0.631 ± 0.017	1.316TrpArg: 1.316 ± 0.025	0.993TrpSer: 0.993 ± 0.026	1.14TrpThr: 1.14 ± 0.025	0.985TrpVal: 0.985 ± 0.022	0.351TrpTrp: 0.351 ± 0.013	0.346TrpTyr: 0.346 ± 0.014	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.46TyrAla: 2.46 ± 0.035	0.173TyrCys: 0.173 ± 0.009	1.28TyrAsp: 1.28 ± 0.033	1.064TyrGlu: 1.064 ± 0.025	0.6TyrPhe: 0.6 ± 0.017	2.141TyrGly: 2.141 ± 0.037	0.39TyrHis: 0.39 ± 0.012	0.442TyrIle: 0.442 ± 0.015	0.322TyrLys: 0.322 ± 0.012	2.241TyrLeu: 2.241 ± 0.033	0.216TyrMet: 0.216 ± 0.01	0.395TyrAsn: 0.395 ± 0.016	1.108TyrPro: 1.108 ± 0.024	0.63TyrGln: 0.63 ± 0.016	1.974TyrArg: 1.974 ± 0.034	0.919TyrSer: 0.919 ± 0.022	1.155TyrThr: 1.155 ± 0.026	1.414TyrVal: 1.414 ± 0.027	0.367TyrTrp: 0.367 ± 0.014	0.435TyrTyr: 0.435 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7273 proteins (2134324 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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