Agreia sp. Leaf244

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agreia; unclassified Agreia

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3657 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S9DW40|A0A0S9DW40_9MICO Lycopene cyclase OS=Agreia sp. Leaf244 OX=1736305 GN=ASF06_07885 PE=4 SV=1
MM1 pKa = 6.41THH3 pKa = 7.11RR4 pKa = 11.84PFLPLVALASAGLIVLAGCSADD26 pKa = 3.33ATGDD30 pKa = 3.6GGSGGASVDD39 pKa = 4.07GEE41 pKa = 4.26ASPAFAIDD49 pKa = 3.73QDD51 pKa = 4.22FPDD54 pKa = 4.96PDD56 pKa = 4.27VITVDD61 pKa = 3.26GQYY64 pKa = 11.13YY65 pKa = 10.74AFATNSRR72 pKa = 11.84AVNLQYY78 pKa = 10.32ATSPDD83 pKa = 3.76LEE85 pKa = 4.45TWSVSLDD92 pKa = 3.47DD93 pKa = 5.65AFPEE97 pKa = 4.21LPAWAEE103 pKa = 4.24TGRR106 pKa = 11.84TWAPDD111 pKa = 3.27VAALPDD117 pKa = 3.7GRR119 pKa = 11.84YY120 pKa = 9.93ALYY123 pKa = 8.52FTAQEE128 pKa = 4.22RR129 pKa = 11.84EE130 pKa = 4.19SGRR133 pKa = 11.84QCIGVAVAAAPTGPFVSDD151 pKa = 3.17APEE154 pKa = 3.93PLICPVGEE162 pKa = 4.53GGAIDD167 pKa = 3.92ASSFVDD173 pKa = 4.34DD174 pKa = 5.0DD175 pKa = 3.77GTRR178 pKa = 11.84YY179 pKa = 9.94LVWKK183 pKa = 10.58NDD185 pKa = 3.68GNCCGLDD192 pKa = 2.86TWLRR196 pKa = 11.84LSTLTADD203 pKa = 3.79GLAVVGEE210 pKa = 4.17PVDD213 pKa = 4.08LVKK216 pKa = 9.78QTEE219 pKa = 4.15EE220 pKa = 4.05WEE222 pKa = 4.46GDD224 pKa = 3.66LVEE227 pKa = 4.74APVIVRR233 pKa = 11.84HH234 pKa = 5.4GDD236 pKa = 3.22EE237 pKa = 3.95YY238 pKa = 11.88ALFYY242 pKa = 10.76SANDD246 pKa = 3.63YY247 pKa = 11.62GGDD250 pKa = 3.85DD251 pKa = 3.67YY252 pKa = 12.28AMGYY256 pKa = 10.8ALAPSIAGPYY266 pKa = 10.12AKK268 pKa = 10.42GDD270 pKa = 3.79GPLLTTATSGGRR282 pKa = 11.84ALGPGGQDD290 pKa = 2.81VVAAPDD296 pKa = 3.69GSDD299 pKa = 3.17RR300 pKa = 11.84IVYY303 pKa = 9.47HH304 pKa = 6.18SWDD307 pKa = 3.02PAYY310 pKa = 10.24SYY312 pKa = 11.2RR313 pKa = 11.84GMIVSPLDD321 pKa = 3.26WSDD324 pKa = 3.28GKK326 pKa = 10.13PVVTPP331 pKa = 4.36

Molecular weight: 34.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S9DNU5|A0A0S9DNU5_9MICO Acetamidase OS=Agreia sp. Leaf244 OX=1736305 GN=ASF06_13340 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.08VRR4 pKa = 11.84NSIKK8 pKa = 10.54SMKK11 pKa = 9.89KK12 pKa = 8.04MPGSQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VFVINKK30 pKa = 8.59LNPRR34 pKa = 11.84FKK36 pKa = 10.79ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight: 4.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3657

0

3657

1181973

38

2527

323.2

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.799 ± 0.053

0.463 ± 0.009

6.313 ± 0.031

5.473 ± 0.038

3.315 ± 0.027

8.716 ± 0.032

1.878 ± 0.022

4.863 ± 0.031

2.095 ± 0.031

10.163 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.72 ± 0.014

2.197 ± 0.018

5.274 ± 0.026

2.815 ± 0.021

6.698 ± 0.046

6.522 ± 0.031

6.269 ± 0.039

8.924 ± 0.037

1.44 ± 0.018

2.062 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski