Amino acid dipepetide frequency for Agreia sp. Leaf244

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.261AlaAla: 18.261 ± 0.166	0.581AlaCys: 0.581 ± 0.023	7.823AlaAsp: 7.823 ± 0.092	7.452AlaGlu: 7.452 ± 0.097	3.944AlaPhe: 3.944 ± 0.062	11.411AlaGly: 11.411 ± 0.095	2.239AlaHis: 2.239 ± 0.051	6.129AlaIle: 6.129 ± 0.078	2.713AlaLys: 2.713 ± 0.058	13.56AlaLeu: 13.56 ± 0.143	2.39AlaMet: 2.39 ± 0.046	2.404AlaAsn: 2.404 ± 0.052	6.152AlaPro: 6.152 ± 0.08	3.554AlaGln: 3.554 ± 0.063	8.346AlaArg: 8.346 ± 0.125	8.5AlaSer: 8.5 ± 0.097	7.427AlaThr: 7.427 ± 0.093	11.031AlaVal: 11.031 ± 0.107	1.73AlaTrp: 1.73 ± 0.038	2.336AlaTyr: 2.336 ± 0.042	0.003AlaXaa: 0.003 ± 0.001
Cys	0.596CysAla: 0.596 ± 0.023	0.041CysCys: 0.041 ± 0.006	0.303CysAsp: 0.303 ± 0.017	0.207CysGlu: 0.207 ± 0.015	0.153CysPhe: 0.153 ± 0.012	0.486CysGly: 0.486 ± 0.02	0.102CysHis: 0.102 ± 0.01	0.209CysIle: 0.209 ± 0.012	0.052CysLys: 0.052 ± 0.007	0.429CysLeu: 0.429 ± 0.02	0.07CysMet: 0.07 ± 0.008	0.089CysAsn: 0.089 ± 0.01	0.261CysPro: 0.261 ± 0.017	0.119CysGln: 0.119 ± 0.01	0.25CysArg: 0.25 ± 0.017	0.357CysSer: 0.357 ± 0.018	0.32CysThr: 0.32 ± 0.021	0.427CysVal: 0.427 ± 0.019	0.063CysTrp: 0.063 ± 0.007	0.096CysTyr: 0.096 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.724AspAla: 8.724 ± 0.107	0.248AspCys: 0.248 ± 0.016	4.553AspAsp: 4.553 ± 0.076	4.569AspGlu: 4.569 ± 0.065	1.939AspPhe: 1.939 ± 0.043	6.037AspGly: 6.037 ± 0.087	1.141AspHis: 1.141 ± 0.033	2.639AspIle: 2.639 ± 0.05	1.13AspLys: 1.13 ± 0.035	5.971AspLeu: 5.971 ± 0.066	0.842AspMet: 0.842 ± 0.024	1.118AspAsn: 1.118 ± 0.036	3.954AspPro: 3.954 ± 0.063	1.546AspGln: 1.546 ± 0.037	4.152AspArg: 4.152 ± 0.06	3.532AspSer: 3.532 ± 0.051	3.223AspThr: 3.223 ± 0.059	5.34AspVal: 5.34 ± 0.069	0.959AspTrp: 0.959 ± 0.028	1.512AspTyr: 1.512 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	6.569GluAla: 6.569 ± 0.09	0.218GluCys: 0.218 ± 0.013	2.214GluAsp: 2.214 ± 0.039	2.498GluGlu: 2.498 ± 0.051	1.849GluPhe: 1.849 ± 0.041	3.849GluGly: 3.849 ± 0.06	1.354GluHis: 1.354 ± 0.04	2.7GluIle: 2.7 ± 0.048	1.59GluLys: 1.59 ± 0.042	6.85GluLeu: 6.85 ± 0.079	0.931GluMet: 0.931 ± 0.028	1.558GluAsn: 1.558 ± 0.039	2.976GluPro: 2.976 ± 0.061	2.194GluGln: 2.194 ± 0.042	4.427GluArg: 4.427 ± 0.08	3.419GluSer: 3.419 ± 0.051	3.163GluThr: 3.163 ± 0.054	4.341GluVal: 4.341 ± 0.06	0.881GluTrp: 0.881 ± 0.025	1.146GluTyr: 1.146 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.292PheAla: 4.292 ± 0.065	0.185PheCys: 0.185 ± 0.014	2.65PheAsp: 2.65 ± 0.047	1.846PheGlu: 1.846 ± 0.042	1.198PhePhe: 1.198 ± 0.036	3.591PheGly: 3.591 ± 0.062	0.538PheHis: 0.538 ± 0.022	1.433PheIle: 1.433 ± 0.035	0.501PheLys: 0.501 ± 0.02	2.833PheLeu: 2.833 ± 0.061	0.482PheMet: 0.482 ± 0.02	0.711PheAsn: 0.711 ± 0.026	1.44PhePro: 1.44 ± 0.035	0.775PheGln: 0.775 ± 0.027	1.767PheArg: 1.767 ± 0.044	2.202PheSer: 2.202 ± 0.038	2.282PheThr: 2.282 ± 0.054	3.154PheVal: 3.154 ± 0.056	0.535PheTrp: 0.535 ± 0.024	0.733PheTyr: 0.733 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.117GlyAla: 10.117 ± 0.104	0.544GlyCys: 0.544 ± 0.023	5.176GlyAsp: 5.176 ± 0.078	4.556GlyGlu: 4.556 ± 0.061	3.371GlyPhe: 3.371 ± 0.058	7.278GlyGly: 7.278 ± 0.084	1.801GlyHis: 1.801 ± 0.044	4.856GlyIle: 4.856 ± 0.068	2.031GlyLys: 2.031 ± 0.05	8.975GlyLeu: 8.975 ± 0.099	1.693GlyMet: 1.693 ± 0.044	1.858GlyAsn: 1.858 ± 0.043	3.692GlyPro: 3.692 ± 0.057	2.489GlyGln: 2.489 ± 0.058	5.807GlyArg: 5.807 ± 0.073	6.015GlySer: 6.015 ± 0.078	5.481GlyThr: 5.481 ± 0.085	7.676GlyVal: 7.676 ± 0.092	1.471GlyTrp: 1.471 ± 0.038	2.268GlyTyr: 2.268 ± 0.05	0.002GlyXaa: 0.002 ± 0.001
His	2.152HisAla: 2.152 ± 0.049	0.106HisCys: 0.106 ± 0.01	1.378HisAsp: 1.378 ± 0.038	1.06HisGlu: 1.06 ± 0.029	0.571HisPhe: 0.571 ± 0.026	1.808HisGly: 1.808 ± 0.042	0.5HisHis: 0.5 ± 0.024	0.76HisIle: 0.76 ± 0.025	0.247HisLys: 0.247 ± 0.014	1.931HisLeu: 1.931 ± 0.048	0.286HisMet: 0.286 ± 0.014	0.392HisAsn: 0.392 ± 0.021	1.424HisPro: 1.424 ± 0.038	0.486HisGln: 0.486 ± 0.02	1.372HisArg: 1.372 ± 0.041	1.121HisSer: 1.121 ± 0.035	0.972HisThr: 0.972 ± 0.03	1.493HisVal: 1.493 ± 0.038	0.287HisTrp: 0.287 ± 0.015	0.431HisTyr: 0.431 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.989IleAla: 6.989 ± 0.084	0.258IleCys: 0.258 ± 0.013	3.884IleAsp: 3.884 ± 0.056	3.109IleGlu: 3.109 ± 0.056	1.36IlePhe: 1.36 ± 0.037	4.674IleGly: 4.674 ± 0.066	0.72IleHis: 0.72 ± 0.027	2.037IleIle: 2.037 ± 0.047	0.906IleLys: 0.906 ± 0.031	4.057IleLeu: 4.057 ± 0.064	0.673IleMet: 0.673 ± 0.025	0.993IleAsn: 0.993 ± 0.028	2.568IlePro: 2.568 ± 0.045	1.082IleGln: 1.082 ± 0.034	2.848IleArg: 2.848 ± 0.052	2.868IleSer: 2.868 ± 0.045	3.037IleThr: 3.037 ± 0.057	5.134IleVal: 5.134 ± 0.063	0.545IleTrp: 0.545 ± 0.02	0.887IleTyr: 0.887 ± 0.028	0.001IleXaa: 0.001 ± 0.001
Lys	2.614LysAla: 2.614 ± 0.059	0.061LysCys: 0.061 ± 0.008	1.069LysAsp: 1.069 ± 0.035	0.939LysGlu: 0.939 ± 0.032	0.565LysPhe: 0.565 ± 0.02	1.514LysGly: 1.514 ± 0.042	0.43LysHis: 0.43 ± 0.023	0.987LysIle: 0.987 ± 0.035	0.885LysLys: 0.885 ± 0.038	1.933LysLeu: 1.933 ± 0.049	0.401LysMet: 0.401 ± 0.02	0.668LysAsn: 0.668 ± 0.027	1.264LysPro: 1.264 ± 0.037	0.777LysGln: 0.777 ± 0.024	1.526LysArg: 1.526 ± 0.041	1.355LysSer: 1.355 ± 0.035	1.551LysThr: 1.551 ± 0.04	1.678LysVal: 1.678 ± 0.047	0.261LysTrp: 0.261 ± 0.015	0.478LysTyr: 0.478 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.131LeuAla: 14.131 ± 0.127	0.458LeuCys: 0.458 ± 0.021	7.065LeuAsp: 7.065 ± 0.089	5.358LeuGlu: 5.358 ± 0.074	3.075LeuPhe: 3.075 ± 0.062	9.295LeuGly: 9.295 ± 0.093	1.79LeuHis: 1.79 ± 0.046	4.756LeuIle: 4.756 ± 0.066	1.92LeuLys: 1.92 ± 0.041	10.182LeuLeu: 10.182 ± 0.132	1.623LeuMet: 1.623 ± 0.038	1.962LeuAsn: 1.962 ± 0.04	5.35LeuPro: 5.35 ± 0.067	2.601LeuGln: 2.601 ± 0.05	6.663LeuArg: 6.663 ± 0.086	6.542LeuSer: 6.542 ± 0.082	6.281LeuThr: 6.281 ± 0.082	9.723LeuVal: 9.723 ± 0.112	1.174LeuTrp: 1.174 ± 0.037	1.683LeuTyr: 1.683 ± 0.042	0.001LeuXaa: 0.001 ± 0.001
Met	2.009MetAla: 2.009 ± 0.04	0.081MetCys: 0.081 ± 0.007	0.758MetAsp: 0.758 ± 0.023	0.602MetGlu: 0.602 ± 0.025	0.492MetPhe: 0.492 ± 0.024	1.169MetGly: 1.169 ± 0.035	0.346MetHis: 0.346 ± 0.017	0.905MetIle: 0.905 ± 0.03	0.467MetLys: 0.467 ± 0.02	1.863MetLeu: 1.863 ± 0.042	0.276MetMet: 0.276 ± 0.016	0.494MetAsn: 0.494 ± 0.021	1.118MetPro: 1.118 ± 0.031	0.525MetGln: 0.525 ± 0.023	1.338MetArg: 1.338 ± 0.035	1.43MetSer: 1.43 ± 0.037	1.646MetThr: 1.646 ± 0.037	1.232MetVal: 1.232 ± 0.03	0.184MetTrp: 0.184 ± 0.013	0.261MetTyr: 0.261 ± 0.016	0.001MetXaa: 0.001 ± 0.001
Asn	2.574AsnAla: 2.574 ± 0.046	0.113AsnCys: 0.113 ± 0.01	1.43AsnAsp: 1.43 ± 0.036	1.146AsnGlu: 1.146 ± 0.031	0.77AsnPhe: 0.77 ± 0.026	2.086AsnGly: 2.086 ± 0.05	0.39AsnHis: 0.39 ± 0.018	0.982AsnIle: 0.982 ± 0.032	0.446AsnLys: 0.446 ± 0.022	1.995AsnLeu: 1.995 ± 0.043	0.372AsnMet: 0.372 ± 0.017	0.56AsnAsn: 0.56 ± 0.022	1.758AsnPro: 1.758 ± 0.039	0.607AsnGln: 0.607 ± 0.026	1.397AsnArg: 1.397 ± 0.033	1.294AsnSer: 1.294 ± 0.036	1.338AsnThr: 1.338 ± 0.033	1.784AsnVal: 1.784 ± 0.039	0.329AsnTrp: 0.329 ± 0.016	0.599AsnTyr: 0.599 ± 0.025	0.002AsnXaa: 0.002 ± 0.001
Pro	6.779ProAla: 6.779 ± 0.098	0.186ProCys: 0.186 ± 0.013	3.922ProAsp: 3.922 ± 0.066	3.597ProGlu: 3.597 ± 0.06	1.738ProPhe: 1.738 ± 0.038	4.753ProGly: 4.753 ± 0.07	0.992ProHis: 0.992 ± 0.027	2.25ProIle: 2.25 ± 0.048	1.036ProLys: 1.036 ± 0.03	4.798ProLeu: 4.798 ± 0.068	0.83ProMet: 0.83 ± 0.026	1.206ProAsn: 1.206 ± 0.03	2.074ProPro: 2.074 ± 0.047	1.606ProGln: 1.606 ± 0.041	3.128ProArg: 3.128 ± 0.059	3.542ProSer: 3.542 ± 0.062	3.487ProThr: 3.487 ± 0.053	4.948ProVal: 4.948 ± 0.076	0.822ProTrp: 0.822 ± 0.024	1.041ProTyr: 1.041 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.591GlnAla: 3.591 ± 0.067	0.114GlnCys: 0.114 ± 0.01	1.108GlnAsp: 1.108 ± 0.033	1.074GlnGlu: 1.074 ± 0.031	0.932GlnPhe: 0.932 ± 0.031	2.108GlnGly: 2.108 ± 0.039	0.604GlnHis: 0.604 ± 0.023	1.454GlnIle: 1.454 ± 0.036	0.773GlnLys: 0.773 ± 0.025	3.44GlnLeu: 3.44 ± 0.055	0.481GlnMet: 0.481 ± 0.019	0.844GlnAsn: 0.844 ± 0.029	1.519GlnPro: 1.519 ± 0.039	1.206GlnGln: 1.206 ± 0.038	2.097GlnArg: 2.097 ± 0.045	1.685GlnSer: 1.685 ± 0.036	1.662GlnThr: 1.662 ± 0.043	2.355GlnVal: 2.355 ± 0.045	0.431GlnTrp: 0.431 ± 0.018	0.67GlnTyr: 0.67 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	7.877ArgAla: 7.877 ± 0.099	0.248ArgCys: 0.248 ± 0.015	3.919ArgAsp: 3.919 ± 0.05	3.821ArgGlu: 3.821 ± 0.066	2.439ArgPhe: 2.439 ± 0.044	4.844ArgGly: 4.844 ± 0.066	1.442ArgHis: 1.442 ± 0.04	3.677ArgIle: 3.677 ± 0.056	1.201ArgLys: 1.201 ± 0.037	7.101ArgLeu: 7.101 ± 0.1	1.522ArgMet: 1.522 ± 0.034	1.333ArgAsn: 1.333 ± 0.031	3.409ArgPro: 3.409 ± 0.064	2.012ArgGln: 2.012 ± 0.038	5.908ArgArg: 5.908 ± 0.084	4.424ArgSer: 4.424 ± 0.059	3.819ArgThr: 3.819 ± 0.061	5.522ArgVal: 5.522 ± 0.075	0.998ArgTrp: 0.998 ± 0.028	1.464ArgTyr: 1.464 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	7.851SerAla: 7.851 ± 0.092	0.279SerCys: 0.279 ± 0.018	3.868SerAsp: 3.868 ± 0.057	3.152SerGlu: 3.152 ± 0.056	2.31SerPhe: 2.31 ± 0.052	6.404SerGly: 6.404 ± 0.082	1.145SerHis: 1.145 ± 0.031	3.356SerIle: 3.356 ± 0.063	1.373SerLys: 1.373 ± 0.031	6.328SerLeu: 6.328 ± 0.076	1.264SerMet: 1.264 ± 0.032	1.493SerAsn: 1.493 ± 0.04	3.372SerPro: 3.372 ± 0.053	1.641SerGln: 1.641 ± 0.039	4.196SerArg: 4.196 ± 0.071	4.678SerSer: 4.678 ± 0.068	4.584SerThr: 4.584 ± 0.07	5.388SerVal: 5.388 ± 0.077	1.069SerTrp: 1.069 ± 0.03	1.472SerTyr: 1.472 ± 0.036	0.001SerXaa: 0.001 ± 0.001
Thr	7.7ThrAla: 7.7 ± 0.09	0.257ThrCys: 0.257 ± 0.016	3.803ThrAsp: 3.803 ± 0.067	3.176ThrGlu: 3.176 ± 0.054	2.035ThrPhe: 2.035 ± 0.046	5.78ThrGly: 5.78 ± 0.092	1.058ThrHis: 1.058 ± 0.032	3.237ThrIle: 3.237 ± 0.058	1.305ThrLys: 1.305 ± 0.042	6.195ThrLeu: 6.195 ± 0.074	0.988ThrMet: 0.988 ± 0.029	1.385ThrAsn: 1.385 ± 0.041	4.13ThrPro: 4.13 ± 0.066	1.534ThrGln: 1.534 ± 0.036	3.739ThrArg: 3.739 ± 0.061	4.0ThrSer: 4.0 ± 0.065	4.237ThrThr: 4.237 ± 0.074	6.146ThrVal: 6.146 ± 0.106	0.875ThrTrp: 0.875 ± 0.025	1.112ThrTyr: 1.112 ± 0.03	0.001ThrXaa: 0.001 ± 0.001
Val	11.324ValAla: 11.324 ± 0.113	0.459ValCys: 0.459 ± 0.019	6.138ValAsp: 6.138 ± 0.08	4.891ValGlu: 4.891 ± 0.068	3.053ValPhe: 3.053 ± 0.053	7.144ValGly: 7.144 ± 0.097	1.57ValHis: 1.57 ± 0.039	4.701ValIle: 4.701 ± 0.077	1.647ValLys: 1.647 ± 0.044	9.273ValLeu: 9.273 ± 0.107	1.464ValMet: 1.464 ± 0.034	1.858ValAsn: 1.858 ± 0.04	4.462ValPro: 4.462 ± 0.065	2.154ValGln: 2.154 ± 0.041	5.384ValArg: 5.384 ± 0.079	5.8ValSer: 5.8 ± 0.065	6.034ValThr: 6.034 ± 0.08	9.111ValVal: 9.111 ± 0.109	1.14ValTrp: 1.14 ± 0.037	1.629ValTyr: 1.629 ± 0.037	0.001ValXaa: 0.001 ± 0.001
Trp	1.444TrpAla: 1.444 ± 0.036	0.074TrpCys: 0.074 ± 0.008	0.657TrpAsp: 0.657 ± 0.021	0.534TrpGlu: 0.534 ± 0.02	0.582TrpPhe: 0.582 ± 0.025	0.998TrpGly: 0.998 ± 0.028	0.314TrpHis: 0.314 ± 0.016	0.77TrpIle: 0.77 ± 0.023	0.35TrpLys: 0.35 ± 0.017	1.69TrpLeu: 1.69 ± 0.039	0.332TrpMet: 0.332 ± 0.016	0.539TrpAsn: 0.539 ± 0.02	0.745TrpPro: 0.745 ± 0.025	0.67TrpGln: 0.67 ± 0.025	1.08TrpArg: 1.08 ± 0.029	1.05TrpSer: 1.05 ± 0.028	0.884TrpThr: 0.884 ± 0.029	1.08TrpVal: 1.08 ± 0.028	0.324TrpTrp: 0.324 ± 0.02	0.286TrpTyr: 0.286 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.39TyrAla: 2.39 ± 0.052	0.118TyrCys: 0.118 ± 0.01	1.411TyrAsp: 1.411 ± 0.039	1.139TyrGlu: 1.139 ± 0.032	0.771TyrPhe: 0.771 ± 0.031	1.927TyrGly: 1.927 ± 0.042	0.301TyrHis: 0.301 ± 0.017	0.793TyrIle: 0.793 ± 0.03	0.381TyrLys: 0.381 ± 0.021	2.23TyrLeu: 2.23 ± 0.045	0.278TyrMet: 0.278 ± 0.017	0.509TyrAsn: 0.509 ± 0.024	1.066TyrPro: 1.066 ± 0.033	0.572TyrGln: 0.572 ± 0.023	1.554TyrArg: 1.554 ± 0.04	1.407TyrSer: 1.407 ± 0.038	1.262TyrThr: 1.262 ± 0.038	1.672TyrVal: 1.672 ± 0.042	0.323TyrTrp: 0.323 ± 0.013	0.514TyrTyr: 0.514 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.003XaaSer: 0.003 ± 0.002	0.002XaaThr: 0.002 ± 0.002	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3657 proteins (1181973 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---