Mycolicibacterium fallax (Mycobacterium fallax)
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3787 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X1R7U7|A0A1X1R7U7_MYCFA Uncharacterized protein OS=Mycolicibacterium fallax OX=1793 GN=AWC04_15025 PE=4 SV=1
MM1 pKa = 7.94 DD2 pKa = 5.11 NDD4 pKa = 4.06 IIALIDD10 pKa = 3.35 QALDD14 pKa = 3.19 EE15 pKa = 4.94 GEE17 pKa = 4.69 PYY19 pKa = 10.8 SPADD23 pKa = 5.72 DD24 pKa = 4.31 PYY26 pKa = 10.28 SWNDD30 pKa = 3.04 AAVWTSTGDD39 pKa = 3.39 HH40 pKa = 6.47 QDD42 pKa = 3.13 RR43 pKa = 11.84 DD44 pKa = 3.65 DD45 pKa = 5.06 DD46 pKa = 4.01 EE47 pKa = 7.21 RR48 pKa = 11.84 DD49 pKa = 3.82 DD50 pKa = 4.36 EE51 pKa = 4.65 VDD53 pKa = 3.06 PLRR56 pKa = 11.84 YY57 pKa = 9.54 VDD59 pKa = 4.8 NPFAGTYY66 pKa = 8.95 YY67 pKa = 10.2 QDD69 pKa = 3.98 PSDD72 pKa = 4.61 PPWLVTPVGHH82 pKa = 5.47 QHH84 pKa = 6.9 IYY86 pKa = 11.09 DD87 pKa = 3.83 HH88 pKa = 7.61 DD89 pKa = 4.17 EE90 pKa = 4.0 PAPAVWEE97 pKa = 4.49 VSRR100 pKa = 11.84 QPPGCYY106 pKa = 9.17 SDD108 pKa = 5.01 EE109 pKa = 4.35 PQAGQTVVLSATLSDD124 pKa = 4.14 GLRR127 pKa = 11.84 TCYY130 pKa = 8.76 FTYY133 pKa = 10.93 VHH135 pKa = 6.97 GEE137 pKa = 4.08 DD138 pKa = 4.08 PQHH141 pKa = 6.8 GEE143 pKa = 3.57 WDD145 pKa = 3.44 GDD147 pKa = 3.5 ADD149 pKa = 3.5 MLEE152 pKa = 4.2 AVRR155 pKa = 11.84 ILGALDD161 pKa = 4.35 PPDD164 pKa = 4.74 DD165 pKa = 4.66 PLRR168 pKa = 11.84 MIAGALARR176 pKa = 11.84 VGWWGTVSDD185 pKa = 4.81 SVWQVLAGDD194 pKa = 4.95 DD195 pKa = 3.88 YY196 pKa = 12.2
Molecular weight: 21.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.643
IPC_protein 3.681
Toseland 3.439
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.503
EMBOSS 3.668
Sillero 3.808
Patrickios 0.693
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|A0A1X1RG22|A0A1X1RG22_MYCFA MarR family transcriptional regulator OS=Mycolicibacterium fallax OX=1793 GN=AWC04_07295 PE=4 SV=1
MM1 pKa = 7.03 QRR3 pKa = 11.84 RR4 pKa = 11.84 NRR6 pKa = 11.84 RR7 pKa = 11.84 AGVEE11 pKa = 4.03 DD12 pKa = 3.19 RR13 pKa = 11.84 WRR15 pKa = 11.84 RR16 pKa = 11.84 SDD18 pKa = 3.37 GSPTARR24 pKa = 11.84 SGMGRR29 pKa = 11.84 RR30 pKa = 11.84 WLARR34 pKa = 11.84 YY35 pKa = 9.79 VDD37 pKa = 3.94 DD38 pKa = 5.11 QGGEE42 pKa = 4.0 NTRR45 pKa = 11.84 SFDD48 pKa = 4.05 RR49 pKa = 11.84 KK50 pKa = 9.99 VDD52 pKa = 3.37 AQAFLNEE59 pKa = 3.63 ITAAQTIGTYY69 pKa = 9.19 VAPKK73 pKa = 9.75 AGRR76 pKa = 11.84 ITVRR80 pKa = 11.84 EE81 pKa = 4.06 LHH83 pKa = 6.31 GKK85 pKa = 8.01 WLGTQGHH92 pKa = 6.39 LKK94 pKa = 8.21 EE95 pKa = 4.48 TTVATRR101 pKa = 11.84 AFAWSGYY108 pKa = 9.27 VEE110 pKa = 4.29 GRR112 pKa = 11.84 WAAVAVADD120 pKa = 4.31 VQSSDD125 pKa = 2.39 IRR127 pKa = 11.84 AWVQQLAAGGAKK139 pKa = 9.81 PATIEE144 pKa = 3.92 NALSVLRR151 pKa = 11.84 QILEE155 pKa = 3.92 MAVDD159 pKa = 3.92 DD160 pKa = 5.0 RR161 pKa = 11.84 RR162 pKa = 11.84 IPRR165 pKa = 11.84 NPCTGVKK172 pKa = 9.7 SPRR175 pKa = 11.84 RR176 pKa = 11.84 QHH178 pKa = 6.43 RR179 pKa = 11.84 ARR181 pKa = 11.84 GYY183 pKa = 8.21 LTHH186 pKa = 6.17 QQVEE190 pKa = 4.16 LLARR194 pKa = 11.84 EE195 pKa = 4.12 VGEE198 pKa = 4.04 YY199 pKa = 10.71 AVVVRR204 pKa = 11.84 FLAYY208 pKa = 9.27 TGLRR212 pKa = 11.84 WGEE215 pKa = 3.76 MAALRR220 pKa = 11.84 VEE222 pKa = 4.32 SFDD225 pKa = 3.24 MLRR228 pKa = 11.84 RR229 pKa = 11.84 RR230 pKa = 11.84 VNIRR234 pKa = 11.84 EE235 pKa = 3.81 AVAEE239 pKa = 4.26 VKK241 pKa = 10.74 GRR243 pKa = 11.84 VVWSSPKK250 pKa = 8.22 SHH252 pKa = 6.39 EE253 pKa = 4.18 RR254 pKa = 11.84 RR255 pKa = 11.84 SVPFPAFLADD265 pKa = 3.7 PLAAIMIGKK274 pKa = 9.37 RR275 pKa = 11.84 RR276 pKa = 11.84 DD277 pKa = 3.61 DD278 pKa = 4.18 LVFTSPGGALLRR290 pKa = 11.84 VSTWRR295 pKa = 11.84 PRR297 pKa = 11.84 VFNMAVQRR305 pKa = 11.84 LQEE308 pKa = 4.53 ADD310 pKa = 3.23 PAYY313 pKa = 8.32 PTVTPHH319 pKa = 7.59 DD320 pKa = 4.26 LRR322 pKa = 11.84 HH323 pKa = 5.48 TAASLSISAGANVKK337 pKa = 9.86 AVQTMLGHH345 pKa = 6.81 ASAVLTLDD353 pKa = 3.76 TYY355 pKa = 12.12 ADD357 pKa = 4.59 LFPDD361 pKa = 4.06 DD362 pKa = 4.89 LEE364 pKa = 4.43 QVSVALDD371 pKa = 3.57 AARR374 pKa = 11.84 MRR376 pKa = 11.84 SLAATADD383 pKa = 3.49 QLRR386 pKa = 11.84 TGKK389 pKa = 10.35
Molecular weight: 43.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.56
IPC_protein 10.584
Toseland 10.643
ProMoST 10.511
Dawson 10.745
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 10.774
Grimsley 10.804
Solomon 10.906
Lehninger 10.862
Nozaki 10.628
DTASelect 10.511
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.687
Patrickios 10.452
IPC_peptide 10.921
IPC2_peptide 9.502
IPC2.peptide.svr19 8.757
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3787
0
3787
1219274
29
3072
322.0
34.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.042 ± 0.057
0.777 ± 0.009
6.321 ± 0.033
5.193 ± 0.029
2.886 ± 0.022
9.183 ± 0.042
2.063 ± 0.021
4.147 ± 0.026
2.017 ± 0.029
10.068 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.974 ± 0.017
2.122 ± 0.019
6.058 ± 0.04
2.851 ± 0.021
7.452 ± 0.041
4.91 ± 0.027
5.955 ± 0.027
8.42 ± 0.034
1.472 ± 0.017
2.089 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here