Flavobacterium sp. 102
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3318 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A495KNL4|A0A495KNL4_9FLAO Dihydroorotase OS=Flavobacterium sp. 102 OX=2135623 GN=C8C84_2407 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.52 KK3 pKa = 10.2 NLFFLLFIAFLTTNCFSQFSKK24 pKa = 8.35 THH26 pKa = 6.21 YY27 pKa = 9.98 IPPLSGSSNVTAEE40 pKa = 4.07 DD41 pKa = 3.28 QFIYY45 pKa = 10.54 ISTPNINPINFRR57 pKa = 11.84 IIEE60 pKa = 4.2 LGGSVINGSVSRR72 pKa = 11.84 STPFIYY78 pKa = 10.64 NIGFGNDD85 pKa = 3.19 TQLHH89 pKa = 5.47 VDD91 pKa = 3.93 ASQTNNTFSNKK102 pKa = 9.49 GYY104 pKa = 10.18 IIEE107 pKa = 4.9 ADD109 pKa = 3.49 DD110 pKa = 5.02 LIYY113 pKa = 11.18 VSARR117 pKa = 11.84 VTAGNGNHH125 pKa = 6.81 AGEE128 pKa = 4.55 LVSKK132 pKa = 10.68 GKK134 pKa = 10.6 ASLGNRR140 pKa = 11.84 FRR142 pKa = 11.84 IGAITNLTVDD152 pKa = 4.34 NYY154 pKa = 11.39 NDD156 pKa = 2.8 IHH158 pKa = 5.5 TTFISVLASEE168 pKa = 5.02 NNTTVNFSDD177 pKa = 4.75 FKK179 pKa = 11.12 PGVQLINSSTGDD191 pKa = 3.53 SPFSIVLNSGEE202 pKa = 4.39 SYY204 pKa = 10.89 VIAVQGPLNPNRR216 pKa = 11.84 DD217 pKa = 3.58 GLIGSLVTSDD227 pKa = 4.84 KK228 pKa = 10.9 PIALNCGSFGGSNAAGNLDD247 pKa = 3.99 LGFDD251 pKa = 3.68 QIVPAEE257 pKa = 4.17 RR258 pKa = 11.84 INNNEE263 pKa = 3.96 YY264 pKa = 10.54 IFIKK268 pKa = 9.68 STGVDD273 pKa = 2.85 IVEE276 pKa = 4.46 TVLLVADD283 pKa = 3.93 VDD285 pKa = 3.86 NTEE288 pKa = 4.12 VFLSGSTTPDD298 pKa = 3.07 VILNAGNYY306 pKa = 9.92 AVFNGSSFDD315 pKa = 4.23 LNGNLYY321 pKa = 9.95 IRR323 pKa = 11.84 TNQKK327 pKa = 9.19 VFAYY331 pKa = 7.92 QTIGDD336 pKa = 4.23 NSASDD341 pKa = 3.44 QRR343 pKa = 11.84 NQEE346 pKa = 4.12 MFFVPPLSCQTPRR359 pKa = 11.84 IIDD362 pKa = 4.21 NIPSIDD368 pKa = 3.8 YY369 pKa = 10.28 VGSRR373 pKa = 11.84 QFTGRR378 pKa = 11.84 VTIVTKK384 pKa = 10.37 TGASLNFIIDD394 pKa = 3.59 GTNYY398 pKa = 9.56 TLAEE402 pKa = 4.32 LSLITNITGPTNVVGNNNYY421 pKa = 6.19 QTYY424 pKa = 10.57 VITGLSGDD432 pKa = 3.52 VSVFSTEE439 pKa = 3.87 EE440 pKa = 4.17 LYY442 pKa = 11.03 LAAYY446 pKa = 6.32 GTSGAATFGGFYY458 pKa = 10.32 SGFTFEE464 pKa = 5.02 PEE466 pKa = 3.96 VSFTQLDD473 pKa = 4.25 LSQANCIPNTSLSVNTLSPFDD494 pKa = 3.54 TFQWYY499 pKa = 9.88 FNGNIIPSATSSSYY513 pKa = 10.95 VPSTLGPGYY522 pKa = 9.81 YY523 pKa = 9.39 YY524 pKa = 11.24 VSATISNCSLPINSNEE540 pKa = 3.89 IPVSICSIDD549 pKa = 3.6 TDD551 pKa = 4.4 NDD553 pKa = 3.64 GTNNNIDD560 pKa = 4.48 LDD562 pKa = 4.01 NDD564 pKa = 3.22 NDD566 pKa = 5.37 GITNCNEE573 pKa = 3.91 SYY575 pKa = 11.31 GDD577 pKa = 3.44 QGVNLTNTAIGSVNIDD593 pKa = 3.04 NYY595 pKa = 9.91 TNSFTGSVAFSGTGTPSATPIVGDD619 pKa = 3.61 ANGNFVTEE627 pKa = 4.27 AANGKK632 pKa = 6.86 QNKK635 pKa = 9.33 VSFTTSWNNPISLAVEE651 pKa = 4.23 YY652 pKa = 8.5 ATTALGDD659 pKa = 4.05 DD660 pKa = 4.65 LFTTSTEE667 pKa = 3.69 IRR669 pKa = 11.84 ITCPINKK676 pKa = 8.66 TLTILNPDD684 pKa = 3.38 NQVLVDD690 pKa = 3.68 TNYY693 pKa = 11.34 DD694 pKa = 3.68 GIFEE698 pKa = 4.37 SGITEE703 pKa = 3.91 YY704 pKa = 11.3 SSFEE708 pKa = 3.35 IRR710 pKa = 11.84 FRR712 pKa = 11.84 LNDD715 pKa = 3.71 NVPLVAGTGTFSVRR729 pKa = 11.84 GNLIDD734 pKa = 5.52 AIIVTNVNLVDD745 pKa = 3.98 TNTSRR750 pKa = 11.84 AAMRR754 pKa = 11.84 LIATCVPKK762 pKa = 10.89 DD763 pKa = 3.52 SDD765 pKa = 3.74 NDD767 pKa = 4.03 GVPDD771 pKa = 4.3 QIDD774 pKa = 3.34 WDD776 pKa = 4.28 SDD778 pKa = 3.15 NDD780 pKa = 4.31 TIPDD784 pKa = 4.85 FIEE787 pKa = 4.22 SQGQNISPLTNVDD800 pKa = 3.77 TNNDD804 pKa = 4.13 GIDD807 pKa = 3.66 DD808 pKa = 4.37 LFGAGTTAADD818 pKa = 4.11 SDD820 pKa = 4.07 TDD822 pKa = 4.59 GIPNYY827 pKa = 10.61 LDD829 pKa = 4.76 LDD831 pKa = 3.96 SDD833 pKa = 3.79 NDD835 pKa = 4.69 GIFDD839 pKa = 4.49 IIEE842 pKa = 4.33 SGSTGNGSNTNGVTINPVGSNGLDD866 pKa = 3.25 NGLEE870 pKa = 4.03 TALDD874 pKa = 3.68 NGIINYY880 pKa = 8.1 TIADD884 pKa = 3.68 SDD886 pKa = 3.98 ADD888 pKa = 3.69 GVLNYY893 pKa = 9.98 IEE895 pKa = 5.45 IDD897 pKa = 3.92 SDD899 pKa = 4.42 DD900 pKa = 5.01 DD901 pKa = 3.79 GCKK904 pKa = 10.38 DD905 pKa = 3.27 VTEE908 pKa = 5.13 AGFTDD913 pKa = 3.81 TNNDD917 pKa = 4.1 GIVGDD922 pKa = 4.03 DD923 pKa = 3.75 TPTVVANQGQVISSSGYY940 pKa = 8.82 GVPNGDD946 pKa = 3.69 YY947 pKa = 10.07 TISAPISISAHH958 pKa = 5.41 PVDD961 pKa = 3.85 VTTCEE966 pKa = 4.07 LQSATFTITSVTVDD980 pKa = 3.28 SYY982 pKa = 11.22 QWQLSTDD989 pKa = 4.6 DD990 pKa = 3.7 EE991 pKa = 4.83 TSWTDD996 pKa = 3.29 LANNATYY1003 pKa = 10.86 AGVNTVSLTVSNVSPSMVGYY1023 pKa = 9.96 KK1024 pKa = 10.32 YY1025 pKa = 10.44 RR1026 pKa = 11.84 VFINKK1031 pKa = 9.25 NGNACGLYY1039 pKa = 10.26 SEE1041 pKa = 5.62 AATLTTFALPVITTPISLIQCDD1063 pKa = 4.79 DD1064 pKa = 3.57 DD1065 pKa = 3.76 TDD1067 pKa = 5.01 AISVFNLTQKK1077 pKa = 11.17 NDD1079 pKa = 3.89 MISANYY1085 pKa = 10.04 LNEE1088 pKa = 3.94 TFSYY1092 pKa = 8.14 FTSSAAANTEE1102 pKa = 3.79 NSSFEE1107 pKa = 3.83 ITNPLAYY1114 pKa = 9.28 TSGNASVYY1122 pKa = 10.87 ARR1124 pKa = 11.84 VEE1126 pKa = 3.93 NSDD1129 pKa = 3.08 GCFRR1133 pKa = 11.84 VAQINLIVSVTQIPANFVIPNQYY1156 pKa = 10.84 LCDD1159 pKa = 5.0 DD1160 pKa = 4.33 YY1161 pKa = 11.88 LDD1163 pKa = 4.31 AANDD1167 pKa = 3.7 DD1168 pKa = 3.96 RR1169 pKa = 11.84 DD1170 pKa = 4.55 GISGPFNFTAIQNSLAAILPNNTTIKK1196 pKa = 10.24 FYY1198 pKa = 9.72 KK1199 pKa = 10.03 TEE1201 pKa = 4.16 ADD1203 pKa = 4.36 FLAEE1207 pKa = 4.09 TDD1209 pKa = 3.49 ASGNPLAIADD1219 pKa = 3.46 ITNYY1223 pKa = 10.54 RR1224 pKa = 11.84 NIGFTNTQKK1233 pKa = 10.03 IWVRR1237 pKa = 11.84 VDD1239 pKa = 3.14 STIDD1243 pKa = 3.27 NSCFGFKK1250 pKa = 10.16 TFDD1253 pKa = 3.54 VVVEE1257 pKa = 4.26 ALPVANPMNANNLIRR1272 pKa = 11.84 QCDD1275 pKa = 3.86 DD1276 pKa = 3.3 DD1277 pKa = 5.42 HH1278 pKa = 8.0 DD1279 pKa = 4.27 GTFGFDD1285 pKa = 2.89 TSGIQTAVLNGQTGVNVTYY1304 pKa = 10.35 FRR1306 pKa = 11.84 ADD1308 pKa = 3.14 GTQILPFTNPYY1319 pKa = 10.36 NVTGTEE1325 pKa = 4.22 TITIRR1330 pKa = 11.84 VNNNITQTGGQPCYY1344 pKa = 10.85 DD1345 pKa = 3.5 EE1346 pKa = 5.77 VSLQFIVDD1354 pKa = 4.1 DD1355 pKa = 4.32 LPEE1358 pKa = 4.67 AFAVPTTLTTTCDD1371 pKa = 3.57 DD1372 pKa = 3.86 EE1373 pKa = 6.24 ANSIDD1378 pKa = 4.11 QDD1380 pKa = 3.88 GLFDD1384 pKa = 5.22 FDD1386 pKa = 3.66 TSTFLSTIIGTQTGMNVYY1404 pKa = 10.45 YY1405 pKa = 10.53 YY1406 pKa = 10.64 DD1407 pKa = 4.21 EE1408 pKa = 4.9 NNVLIPSSIPNQLPNPFRR1426 pKa = 11.84 TATQNVTVVVEE1437 pKa = 3.84 NAINNSCSVQVILPFVVNPTPKK1459 pKa = 8.83 IDD1461 pKa = 3.57 QEE1463 pKa = 4.26 EE1464 pKa = 4.32 TVIICIPATQVLIDD1478 pKa = 3.88 AGIVDD1483 pKa = 4.85 GTPTSNYY1490 pKa = 7.99 TYY1492 pKa = 9.72 QWYY1495 pKa = 9.38 FNNVIMPGRR1504 pKa = 11.84 TNYY1507 pKa = 10.88 SLIASTPGIYY1517 pKa = 10.16 SVTVTNSFGCSKK1529 pKa = 9.74 TRR1531 pKa = 11.84 IITVNGSEE1539 pKa = 4.14 IATLQSIDD1547 pKa = 3.82 VVDD1550 pKa = 4.87 LSDD1553 pKa = 3.55 INSISVIVSGSGAYY1567 pKa = 9.18 EE1568 pKa = 4.18 FSLDD1572 pKa = 4.88 DD1573 pKa = 4.42 INGPYY1578 pKa = 9.37 QDD1580 pKa = 4.53 SGLFNNVPMGLHH1592 pKa = 5.2 QLYY1595 pKa = 10.25 IRR1597 pKa = 11.84 DD1598 pKa = 3.86 KK1599 pKa = 10.4 NGCGSLGPIGIPVLGIPQYY1618 pKa = 8.02 FTPNGDD1624 pKa = 3.42 GFHH1627 pKa = 7.65 DD1628 pKa = 3.75 YY1629 pKa = 10.88 WNVKK1633 pKa = 8.1 GVSMFSNTNSIIFIFDD1649 pKa = 3.4 RR1650 pKa = 11.84 YY1651 pKa = 10.64 GKK1653 pKa = 10.07 LLKK1656 pKa = 10.07 QLNAFSPGWDD1666 pKa = 2.93 GTYY1669 pKa = 10.73 NGNQMPADD1677 pKa = 4.6 DD1678 pKa = 4.29 YY1679 pKa = 10.83 WYY1681 pKa = 10.51 SIQFDD1686 pKa = 3.53 DD1687 pKa = 3.66 GRR1689 pKa = 11.84 NVKK1692 pKa = 9.68 GHH1694 pKa = 6.4 FALKK1698 pKa = 10.27 RR1699 pKa = 3.54
Molecular weight: 182.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.757
IPC_protein 3.821
Toseland 3.579
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.63
Grimsley 3.478
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.215
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.935
Patrickios 0.515
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|A0A495KLC1|A0A495KLC1_9FLAO FkbM family methyltransferase OS=Flavobacterium sp. 102 OX=2135623 GN=C8C84_1577 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.35 KK16 pKa = 10.31 RR17 pKa = 11.84 KK18 pKa = 9.82 KK19 pKa = 8.65 RR20 pKa = 11.84 ARR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.91 KK27 pKa = 10.59 KK28 pKa = 9.82 KK29 pKa = 10.51 KK30 pKa = 10.04
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3318
0
3318
1143900
29
5181
344.8
38.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.505 ± 0.056
0.867 ± 0.028
5.249 ± 0.037
6.252 ± 0.069
5.352 ± 0.04
6.198 ± 0.056
1.62 ± 0.022
8.038 ± 0.047
7.677 ± 0.098
9.025 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.154 ± 0.027
6.59 ± 0.048
3.405 ± 0.033
3.512 ± 0.025
3.061 ± 0.035
6.61 ± 0.058
6.5 ± 0.118
6.312 ± 0.042
1.01 ± 0.016
4.064 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here