Amino acid dipepetide frequency for Flavobacterium sp. 102

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.293AlaAla: 4.293 ± 0.097	0.673AlaCys: 0.673 ± 0.054	3.283AlaAsp: 3.283 ± 0.058	4.089AlaGlu: 4.089 ± 0.073	3.354AlaPhe: 3.354 ± 0.062	4.069AlaGly: 4.069 ± 0.099	0.952AlaHis: 0.952 ± 0.03	5.491AlaIle: 5.491 ± 0.084	4.896AlaLys: 4.896 ± 0.091	5.976AlaLeu: 5.976 ± 0.086	1.489AlaMet: 1.489 ± 0.042	4.097AlaAsn: 4.097 ± 0.091	1.983AlaPro: 1.983 ± 0.083	2.632AlaGln: 2.632 ± 0.067	1.726AlaArg: 1.726 ± 0.043	4.227AlaSer: 4.227 ± 0.086	4.785AlaThr: 4.785 ± 0.158	4.173AlaVal: 4.173 ± 0.075	0.573AlaTrp: 0.573 ± 0.023	2.297AlaTyr: 2.297 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.532CysAla: 0.532 ± 0.032	0.113CysCys: 0.113 ± 0.01	0.583CysAsp: 0.583 ± 0.048	0.503CysGlu: 0.503 ± 0.025	0.478CysPhe: 0.478 ± 0.02	0.72CysGly: 0.72 ± 0.042	0.219CysHis: 0.219 ± 0.017	0.615CysIle: 0.615 ± 0.027	0.476CysLys: 0.476 ± 0.022	0.728CysLeu: 0.728 ± 0.021	0.117CysMet: 0.117 ± 0.012	0.52CysAsn: 0.52 ± 0.027	0.421CysPro: 0.421 ± 0.032	0.281CysGln: 0.281 ± 0.017	0.205CysArg: 0.205 ± 0.013	0.795CysSer: 0.795 ± 0.046	0.481CysThr: 0.481 ± 0.026	0.471CysVal: 0.471 ± 0.03	0.074CysTrp: 0.074 ± 0.007	0.342CysTyr: 0.342 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.509AspAla: 3.509 ± 0.089	0.452AspCys: 0.452 ± 0.022	2.679AspAsp: 2.679 ± 0.06	3.391AspGlu: 3.391 ± 0.072	3.561AspPhe: 3.561 ± 0.065	3.579AspGly: 3.579 ± 0.071	0.726AspHis: 0.726 ± 0.027	4.145AspIle: 4.145 ± 0.068	4.203AspLys: 4.203 ± 0.087	5.021AspLeu: 5.021 ± 0.073	1.037AspMet: 1.037 ± 0.031	3.425AspAsn: 3.425 ± 0.068	1.324AspPro: 1.324 ± 0.04	1.349AspGln: 1.349 ± 0.035	1.611AspArg: 1.611 ± 0.043	2.927AspSer: 2.927 ± 0.055	2.668AspThr: 2.668 ± 0.077	3.294AspVal: 3.294 ± 0.057	0.75AspTrp: 0.75 ± 0.029	2.844AspTyr: 2.844 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	4.173GluAla: 4.173 ± 0.073	0.377GluCys: 0.377 ± 0.023	3.035GluAsp: 3.035 ± 0.064	4.449GluGlu: 4.449 ± 0.094	2.892GluPhe: 2.892 ± 0.059	3.25GluGly: 3.25 ± 0.059	0.978GluHis: 0.978 ± 0.033	5.603GluIle: 5.603 ± 0.094	6.018GluLys: 6.018 ± 0.114	5.594GluLeu: 5.594 ± 0.107	1.617GluMet: 1.617 ± 0.044	4.693GluAsn: 4.693 ± 0.078	1.525GluPro: 1.525 ± 0.044	2.238GluGln: 2.238 ± 0.06	2.205GluArg: 2.205 ± 0.052	3.143GluSer: 3.143 ± 0.062	3.841GluThr: 3.841 ± 0.064	4.085GluVal: 4.085 ± 0.065	0.535GluTrp: 0.535 ± 0.023	2.272GluTyr: 2.272 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.233PheAla: 3.233 ± 0.064	0.511PheCys: 0.511 ± 0.023	3.32PheAsp: 3.32 ± 0.063	3.612PheGlu: 3.612 ± 0.063	2.867PhePhe: 2.867 ± 0.065	3.658PheGly: 3.658 ± 0.063	0.837PheHis: 0.837 ± 0.03	3.904PheIle: 3.904 ± 0.08	3.632PheLys: 3.632 ± 0.074	4.715PheLeu: 4.715 ± 0.086	1.083PheMet: 1.083 ± 0.034	3.322PheAsn: 3.322 ± 0.061	1.769PhePro: 1.769 ± 0.046	1.715PheGln: 1.715 ± 0.044	1.541PheArg: 1.541 ± 0.039	4.117PheSer: 4.117 ± 0.061	3.535PheThr: 3.535 ± 0.089	3.257PheVal: 3.257 ± 0.057	0.574PheTrp: 0.574 ± 0.026	2.318PheTyr: 2.318 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	3.893GlyAla: 3.893 ± 0.093	0.749GlyCys: 0.749 ± 0.046	3.006GlyAsp: 3.006 ± 0.064	3.137GlyGlu: 3.137 ± 0.061	3.652GlyPhe: 3.652 ± 0.053	4.261GlyGly: 4.261 ± 0.096	1.022GlyHis: 1.022 ± 0.033	5.177GlyIle: 5.177 ± 0.074	4.951GlyLys: 4.951 ± 0.088	5.41GlyLeu: 5.41 ± 0.076	1.509GlyMet: 1.509 ± 0.043	3.941GlyAsn: 3.941 ± 0.089	1.243GlyPro: 1.243 ± 0.04	2.048GlyGln: 2.048 ± 0.054	1.748GlyArg: 1.748 ± 0.047	4.163GlySer: 4.163 ± 0.096	4.602GlyThr: 4.602 ± 0.173	4.082GlyVal: 4.082 ± 0.076	0.671GlyTrp: 0.671 ± 0.027	2.717GlyTyr: 2.717 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.822HisAla: 0.822 ± 0.029	0.194HisCys: 0.194 ± 0.013	0.819HisAsp: 0.819 ± 0.03	0.886HisGlu: 0.886 ± 0.028	1.178HisPhe: 1.178 ± 0.035	0.935HisGly: 0.935 ± 0.033	0.416HisHis: 0.416 ± 0.022	1.192HisIle: 1.192 ± 0.036	1.06HisLys: 1.06 ± 0.033	1.712HisLeu: 1.712 ± 0.042	0.284HisMet: 0.284 ± 0.018	0.935HisAsn: 0.935 ± 0.027	0.768HisPro: 0.768 ± 0.027	0.711HisGln: 0.711 ± 0.026	0.606HisArg: 0.606 ± 0.022	1.063HisSer: 1.063 ± 0.031	0.838HisThr: 0.838 ± 0.032	0.79HisVal: 0.79 ± 0.029	0.194HisTrp: 0.194 ± 0.013	0.797HisTyr: 0.797 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.854IleAla: 5.854 ± 0.089	0.708IleCys: 0.708 ± 0.031	4.712IleAsp: 4.712 ± 0.084	5.355IleGlu: 5.355 ± 0.098	3.569IlePhe: 3.569 ± 0.07	5.055IleGly: 5.055 ± 0.08	1.303IleHis: 1.303 ± 0.035	6.364IleIle: 6.364 ± 0.107	5.716IleLys: 5.716 ± 0.107	6.881IleLeu: 6.881 ± 0.12	1.397IleMet: 1.397 ± 0.038	5.033IleAsn: 5.033 ± 0.073	3.278IlePro: 3.278 ± 0.056	2.75IleGln: 2.75 ± 0.05	2.526IleArg: 2.526 ± 0.052	5.768IleSer: 5.768 ± 0.076	5.332IleThr: 5.332 ± 0.101	5.14IleVal: 5.14 ± 0.084	0.694IleTrp: 0.694 ± 0.024	2.95IleTyr: 2.95 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	5.124LysAla: 5.124 ± 0.098	0.407LysCys: 0.407 ± 0.02	4.029LysAsp: 4.029 ± 0.084	5.639LysGlu: 5.639 ± 0.099	2.943LysPhe: 2.943 ± 0.066	4.209LysGly: 4.209 ± 0.07	1.205LysHis: 1.205 ± 0.038	6.606LysIle: 6.606 ± 0.133	6.905LysLys: 6.905 ± 0.123	6.387LysLeu: 6.387 ± 0.114	2.153LysMet: 2.153 ± 0.055	5.19LysAsn: 5.19 ± 0.101	2.554LysPro: 2.554 ± 0.06	2.664LysGln: 2.664 ± 0.057	2.568LysArg: 2.568 ± 0.054	4.709LysSer: 4.709 ± 0.085	4.874LysThr: 4.874 ± 0.081	4.916LysVal: 4.916 ± 0.095	0.711LysTrp: 0.711 ± 0.025	2.976LysTyr: 2.976 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	5.895LeuAla: 5.895 ± 0.076	0.76LeuCys: 0.76 ± 0.033	4.619LeuAsp: 4.619 ± 0.071	5.636LeuGlu: 5.636 ± 0.101	4.991LeuPhe: 4.991 ± 0.087	5.396LeuGly: 5.396 ± 0.078	1.505LeuHis: 1.505 ± 0.041	6.693LeuIle: 6.693 ± 0.112	7.279LeuLys: 7.279 ± 0.139	8.829LeuLeu: 8.829 ± 0.135	1.994LeuMet: 1.994 ± 0.052	5.756LeuAsn: 5.756 ± 0.068	3.389LeuPro: 3.389 ± 0.065	3.274LeuGln: 3.274 ± 0.067	2.84LeuArg: 2.84 ± 0.056	6.551LeuSer: 6.551 ± 0.093	5.579LeuThr: 5.579 ± 0.1	5.339LeuVal: 5.339 ± 0.077	0.824LeuTrp: 0.824 ± 0.034	3.098LeuTyr: 3.098 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	1.456MetAla: 1.456 ± 0.044	0.12MetCys: 0.12 ± 0.01	1.024MetAsp: 1.024 ± 0.033	1.351MetGlu: 1.351 ± 0.043	0.864MetPhe: 0.864 ± 0.03	1.253MetGly: 1.253 ± 0.035	0.379MetHis: 0.379 ± 0.019	1.66MetIle: 1.66 ± 0.043	2.32MetLys: 2.32 ± 0.048	1.836MetLeu: 1.836 ± 0.048	0.615MetMet: 0.615 ± 0.027	1.393MetAsn: 1.393 ± 0.04	0.844MetPro: 0.844 ± 0.032	0.798MetGln: 0.798 ± 0.027	0.763MetArg: 0.763 ± 0.026	1.439MetSer: 1.439 ± 0.036	1.202MetThr: 1.202 ± 0.036	1.379MetVal: 1.379 ± 0.039	0.145MetTrp: 0.145 ± 0.011	0.696MetTyr: 0.696 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	4.24AsnAla: 4.24 ± 0.071	0.607AsnCys: 0.607 ± 0.042	3.487AsnAsp: 3.487 ± 0.057	3.8AsnGlu: 3.8 ± 0.065	3.408AsnPhe: 3.408 ± 0.061	4.553AsnGly: 4.553 ± 0.103	1.184AsnHis: 1.184 ± 0.037	5.053AsnIle: 5.053 ± 0.076	4.254AsnLys: 4.254 ± 0.078	5.776AsnLeu: 5.776 ± 0.093	1.131AsnMet: 1.131 ± 0.039	4.521AsnAsn: 4.521 ± 0.088	3.206AsnPro: 3.206 ± 0.087	2.637AsnGln: 2.637 ± 0.055	2.053AsnArg: 2.053 ± 0.044	4.34AsnSer: 4.34 ± 0.08	3.972AsnThr: 3.972 ± 0.101	3.864AsnVal: 3.864 ± 0.07	0.802AsnTrp: 0.802 ± 0.029	3.012AsnTyr: 3.012 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.029ProAla: 2.029 ± 0.068	0.235ProCys: 0.235 ± 0.015	1.782ProAsp: 1.782 ± 0.04	2.571ProGlu: 2.571 ± 0.048	1.963ProPhe: 1.963 ± 0.039	1.835ProGly: 1.835 ± 0.056	0.493ProHis: 0.493 ± 0.02	2.918ProIle: 2.918 ± 0.056	2.522ProLys: 2.522 ± 0.06	2.915ProLeu: 2.915 ± 0.066	0.781ProMet: 0.781 ± 0.026	2.594ProAsn: 2.594 ± 0.071	0.793ProPro: 0.793 ± 0.033	1.113ProGln: 1.113 ± 0.038	0.833ProArg: 0.833 ± 0.033	2.257ProSer: 2.257 ± 0.059	2.527ProThr: 2.527 ± 0.095	2.284ProVal: 2.284 ± 0.061	0.269ProTrp: 0.269 ± 0.014	1.332ProTyr: 1.332 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.076GlnAla: 2.076 ± 0.053	0.247GlnCys: 0.247 ± 0.015	1.538GlnAsp: 1.538 ± 0.042	2.215GlnGlu: 2.215 ± 0.052	1.921GlnPhe: 1.921 ± 0.055	1.745GlnGly: 1.745 ± 0.049	0.595GlnHis: 0.595 ± 0.024	2.735GlnIle: 2.735 ± 0.051	3.174GlnLys: 3.174 ± 0.07	3.416GlnLeu: 3.416 ± 0.07	0.853GlnMet: 0.853 ± 0.029	2.63GlnAsn: 2.63 ± 0.062	1.206GlnPro: 1.206 ± 0.043	1.389GlnGln: 1.389 ± 0.045	1.081GlnArg: 1.081 ± 0.035	2.18GlnSer: 2.18 ± 0.052	2.258GlnThr: 2.258 ± 0.064	2.001GlnVal: 2.001 ± 0.05	0.389GlnTrp: 0.389 ± 0.023	1.47GlnTyr: 1.47 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	1.799ArgAla: 1.799 ± 0.045	0.226ArgCys: 0.226 ± 0.017	1.566ArgAsp: 1.566 ± 0.039	1.935ArgGlu: 1.935 ± 0.048	1.781ArgPhe: 1.781 ± 0.045	1.565ArgGly: 1.565 ± 0.044	0.511ArgHis: 0.511 ± 0.022	2.794ArgIle: 2.794 ± 0.05	2.562ArgLys: 2.562 ± 0.064	2.934ArgLeu: 2.934 ± 0.064	0.885ArgMet: 0.885 ± 0.029	1.99ArgAsn: 1.99 ± 0.049	0.896ArgPro: 0.896 ± 0.031	0.958ArgGln: 0.958 ± 0.031	1.131ArgArg: 1.131 ± 0.038	1.632ArgSer: 1.632 ± 0.042	1.8ArgThr: 1.8 ± 0.041	1.915ArgVal: 1.915 ± 0.043	0.35ArgTrp: 0.35 ± 0.019	1.378ArgTyr: 1.378 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	4.117SerAla: 4.117 ± 0.078	0.74SerCys: 0.74 ± 0.035	3.377SerAsp: 3.377 ± 0.052	3.792SerGlu: 3.792 ± 0.073	3.961SerPhe: 3.961 ± 0.059	4.801SerGly: 4.801 ± 0.116	1.074SerHis: 1.074 ± 0.031	5.229SerIle: 5.229 ± 0.073	4.73SerLys: 4.73 ± 0.087	5.945SerLeu: 5.945 ± 0.076	1.283SerMet: 1.283 ± 0.037	4.259SerAsn: 4.259 ± 0.076	2.233SerPro: 2.233 ± 0.059	2.422SerGln: 2.422 ± 0.052	1.878SerArg: 1.878 ± 0.04	4.515SerSer: 4.515 ± 0.094	4.087SerThr: 4.087 ± 0.123	4.331SerVal: 4.331 ± 0.105	0.652SerTrp: 0.652 ± 0.029	2.671SerTyr: 2.671 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	4.597ThrAla: 4.597 ± 0.165	0.485ThrCys: 0.485 ± 0.032	3.321ThrAsp: 3.321 ± 0.08	3.515ThrGlu: 3.515 ± 0.066	3.521ThrPhe: 3.521 ± 0.075	4.446ThrGly: 4.446 ± 0.136	0.931ThrHis: 0.931 ± 0.029	5.642ThrIle: 5.642 ± 0.109	4.014ThrLys: 4.014 ± 0.064	5.767ThrLeu: 5.767 ± 0.1	1.041ThrMet: 1.041 ± 0.033	4.176ThrAsn: 4.176 ± 0.11	2.813ThrPro: 2.813 ± 0.098	2.162ThrGln: 2.162 ± 0.06	1.575ThrArg: 1.575 ± 0.038	4.284ThrSer: 4.284 ± 0.129	4.759ThrThr: 4.759 ± 0.189	4.683ThrVal: 4.683 ± 0.159	0.661ThrTrp: 0.661 ± 0.049	2.601ThrTyr: 2.601 ± 0.093	0.0ThrXaa: 0.0 ± 0.0
Val	4.411ValAla: 4.411 ± 0.091	0.607ValCys: 0.607 ± 0.03	3.514ValAsp: 3.514 ± 0.067	3.769ValGlu: 3.769 ± 0.062	3.439ValPhe: 3.439 ± 0.063	3.704ValGly: 3.704 ± 0.069	0.874ValHis: 0.874 ± 0.033	5.032ValIle: 5.032 ± 0.078	4.473ValLys: 4.473 ± 0.079	5.675ValLeu: 5.675 ± 0.08	1.327ValMet: 1.327 ± 0.038	3.965ValAsn: 3.965 ± 0.084	2.148ValPro: 2.148 ± 0.047	1.872ValGln: 1.872 ± 0.038	1.92ValArg: 1.92 ± 0.045	4.637ValSer: 4.637 ± 0.096	4.488ValThr: 4.488 ± 0.144	4.208ValVal: 4.208 ± 0.07	0.604ValTrp: 0.604 ± 0.019	2.45ValTyr: 2.45 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.594TrpAla: 0.594 ± 0.025	0.101TrpCys: 0.101 ± 0.009	0.538TrpAsp: 0.538 ± 0.021	0.59TrpGlu: 0.59 ± 0.027	0.552TrpPhe: 0.552 ± 0.025	0.536TrpGly: 0.536 ± 0.026	0.209TrpHis: 0.209 ± 0.012	0.739TrpIle: 0.739 ± 0.027	0.781TrpLys: 0.781 ± 0.027	0.943TrpLeu: 0.943 ± 0.038	0.269TrpMet: 0.269 ± 0.015	0.762TrpAsn: 0.762 ± 0.026	0.2TrpPro: 0.2 ± 0.014	0.47TrpGln: 0.47 ± 0.022	0.321TrpArg: 0.321 ± 0.019	0.638TrpSer: 0.638 ± 0.028	0.674TrpThr: 0.674 ± 0.048	0.605TrpVal: 0.605 ± 0.026	0.131TrpTrp: 0.131 ± 0.011	0.446TrpTyr: 0.446 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.408TyrAla: 2.408 ± 0.045	0.364TyrCys: 0.364 ± 0.018	2.261TyrAsp: 2.261 ± 0.044	2.29TyrGlu: 2.29 ± 0.05	2.627TyrPhe: 2.627 ± 0.055	2.412TyrGly: 2.412 ± 0.049	0.785TyrHis: 0.785 ± 0.027	2.795TyrIle: 2.795 ± 0.057	2.804TyrLys: 2.804 ± 0.058	3.788TyrLeu: 3.788 ± 0.066	0.668TyrMet: 0.668 ± 0.028	2.697TyrAsn: 2.697 ± 0.055	1.455TyrPro: 1.455 ± 0.041	1.636TyrGln: 1.636 ± 0.04	1.476TyrArg: 1.476 ± 0.037	2.713TyrSer: 2.713 ± 0.052	2.694TyrThr: 2.694 ± 0.087	2.298TyrVal: 2.298 ± 0.049	0.497TyrTrp: 0.497 ± 0.021	1.972TyrTyr: 1.972 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3318 proteins (1143900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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