Cellulomonas gilvus (strain ATCC 13127 / NRRL B-14078) (Cellvibrio gilvus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; Cellulomonas gilvus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8A7B7|F8A7B7_CELGA Uncharacterized protein OS=Cellulomonas gilvus (strain ATCC 13127 / NRRL B-14078) OX=593907 GN=Celgi_0656 PE=4 SV=1
MM1 pKa = 7.81ASTLAHH7 pKa = 6.29RR8 pKa = 11.84RR9 pKa = 11.84TLRR12 pKa = 11.84AALSLVPIAALAACSLTSTDD32 pKa = 4.98DD33 pKa = 3.42PAAPSTSASAGPSSGAPGTVTLVTHH58 pKa = 7.31DD59 pKa = 4.28SFALADD65 pKa = 4.0GLLEE69 pKa = 5.35DD70 pKa = 5.16FTASTGITVEE80 pKa = 4.32VVQPGDD86 pKa = 3.06AGTLVNQLVLTKK98 pKa = 10.38DD99 pKa = 3.4APLGDD104 pKa = 3.82AVFGIDD110 pKa = 3.17NAFASRR116 pKa = 11.84ALDD119 pKa = 3.57EE120 pKa = 5.35GVLAPYY126 pKa = 9.77TSAAPAAADD135 pKa = 3.54AATHH139 pKa = 6.72ALAGDD144 pKa = 3.88DD145 pKa = 4.22EE146 pKa = 4.92GALTAVDD153 pKa = 4.0YY154 pKa = 11.68GDD156 pKa = 3.35VCLNVDD162 pKa = 3.66HH163 pKa = 7.26AWFDD167 pKa = 3.51EE168 pKa = 4.27HH169 pKa = 7.42DD170 pKa = 4.02VPEE173 pKa = 5.27PEE175 pKa = 4.48TLDD178 pKa = 5.03DD179 pKa = 5.71LLDD182 pKa = 4.0PQYY185 pKa = 11.48EE186 pKa = 4.27DD187 pKa = 4.59LLVVPNPVTSSPGFAFLLTTVAASSDD213 pKa = 2.95GWQDD217 pKa = 3.07YY218 pKa = 8.13WQGLVDD224 pKa = 4.39NGLKK228 pKa = 10.42VADD231 pKa = 4.27GWSDD235 pKa = 3.72AYY237 pKa = 10.15FTDD240 pKa = 4.93FSGGGGDD247 pKa = 3.76GPRR250 pKa = 11.84PIVLSYY256 pKa = 11.15ASSPPSTVPEE266 pKa = 4.83GGTEE270 pKa = 4.01PTTGALLGTCFRR282 pKa = 11.84QVEE285 pKa = 4.42YY286 pKa = 11.18AGVLANAQNPSGAQQLIDD304 pKa = 4.3FLLSDD309 pKa = 4.88AVQADD314 pKa = 4.11IPGSMYY320 pKa = 9.9MYY322 pKa = 9.75PVSTAVEE329 pKa = 5.0LPQEE333 pKa = 4.2WQQWAPLAEE342 pKa = 4.45QPFEE346 pKa = 4.09VAPDD350 pKa = 4.08EE351 pKa = 4.35IAAEE355 pKa = 3.95RR356 pKa = 11.84DD357 pKa = 2.95TWLEE361 pKa = 3.74QWSDD365 pKa = 3.52TVIGG369 pKa = 3.88

Molecular weight: 38.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8A379|F8A379_CELGA Inner-membrane translocator OS=Cellulomonas gilvus (strain ATCC 13127 / NRRL B-14078) OX=593907 GN=Celgi_2573 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3153

0

3153

1067109

32

2381

338.4

35.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.937 ± 0.076

0.567 ± 0.01

6.487 ± 0.032

5.105 ± 0.048

2.421 ± 0.025

9.24 ± 0.038

2.142 ± 0.023

2.767 ± 0.035

1.418 ± 0.029

10.214 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.505 ± 0.017

1.4 ± 0.025

6.03 ± 0.043

2.782 ± 0.024

7.941 ± 0.051

4.814 ± 0.032

6.56 ± 0.056

10.312 ± 0.044

1.586 ± 0.02

1.774 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski