Edwardsiella ictaluri (strain 93-146)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Hafniaceae; Edwardsiella; Edwardsiella ictaluri

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5BEQ5|C5BEQ5_EDWI9 DUF3644 domain-containing protein OS=Edwardsiella ictaluri (strain 93-146) OX=634503 GN=NT01EI_3143 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.16KK3 pKa = 10.09IYY5 pKa = 9.5IHH7 pKa = 7.14KK8 pKa = 10.34DD9 pKa = 2.92NLDD12 pKa = 3.63SYY14 pKa = 10.42TEE16 pKa = 4.11YY17 pKa = 10.36PVPEE21 pKa = 5.01DD22 pKa = 3.3TTDD25 pKa = 2.66WYY27 pKa = 10.13TVDD30 pKa = 3.78VPDD33 pKa = 5.67DD34 pKa = 3.54FTLAGSVYY42 pKa = 9.89NPQIGEE48 pKa = 4.19FDD50 pKa = 3.85TPALPPII57 pKa = 4.37

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5BF72|C5BF72_EDWI9 DUF1471 domain-containing protein OS=Edwardsiella ictaluri (strain 93-146) OX=634503 GN=NT01EI_0420 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.68RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.28GFRR19 pKa = 11.84ARR21 pKa = 11.84MANKK25 pKa = 9.98NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVAGG46 pKa = 4.3

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3686

0

3686

1044215

37

3711

283.3

31.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.46 ± 0.051

1.313 ± 0.02

5.197 ± 0.034

5.052 ± 0.047

3.577 ± 0.029

7.606 ± 0.047

2.416 ± 0.023

5.589 ± 0.037

3.342 ± 0.045

11.479 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.02

3.292 ± 0.033

4.622 ± 0.034

4.915 ± 0.042

6.618 ± 0.042

5.838 ± 0.035

5.02 ± 0.031

6.687 ± 0.034

1.402 ± 0.019

2.854 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski