Actinospica robiniae DSM 44927

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Catenulisporales; Actinospicaceae; Actinospica; Actinospica robiniae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 191 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9DYU4|W9DYU4_9ACTN Acyl-CoA dehydrogenase OS=Actinospica robiniae DSM 44927 OX=479430 GN=ActroDRAFT_0011 PE=3 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84RR3 pKa = 11.84VLTSVISILTATAALSPLGSAAQAADD29 pKa = 3.36ATAPPPLKK37 pKa = 10.24VVTFNTCGQSTACQSTEE54 pKa = 4.95DD55 pKa = 3.81LTDD58 pKa = 3.22WASDD62 pKa = 3.5LSSAILAYY70 pKa = 10.43GPDD73 pKa = 3.34AGSIQEE79 pKa = 4.06VCGNQVDD86 pKa = 4.25AFSADD91 pKa = 3.21LTGYY95 pKa = 7.71TVYY98 pKa = 10.63FNPAWDD104 pKa = 4.58LPTPATTGTNNNHH117 pKa = 6.02CSHH120 pKa = 7.16WASTEE125 pKa = 3.97NEE127 pKa = 4.29DD128 pKa = 4.49EE129 pKa = 4.73FGDD132 pKa = 3.84MIYY135 pKa = 10.75VKK137 pKa = 10.5SSEE140 pKa = 4.38SPVLSTDD147 pKa = 3.49VIYY150 pKa = 7.83PTNPTVEE157 pKa = 4.31SRR159 pKa = 11.84PLEE162 pKa = 4.34CVEE165 pKa = 4.24ATDD168 pKa = 4.77SGMPYY173 pKa = 9.89QYY175 pKa = 10.95CDD177 pKa = 3.07VHH179 pKa = 8.34LDD181 pKa = 3.54GGLGGAPAQGLPEE194 pKa = 4.51VINRR198 pKa = 11.84MQYY201 pKa = 9.2WGGANTPTVLAGDD214 pKa = 4.65FNADD218 pKa = 3.25PTDD221 pKa = 3.83PNMGLIYY228 pKa = 9.96QAQDD232 pKa = 3.1GNGSFIEE239 pKa = 4.12AEE241 pKa = 4.36QYY243 pKa = 11.04DD244 pKa = 4.02KK245 pKa = 11.46AYY247 pKa = 7.59FTPQCRR253 pKa = 11.84QLTSCRR259 pKa = 11.84SGAPTTYY266 pKa = 9.98PVLGSKK272 pKa = 11.14GEE274 pKa = 4.12VAAGGQARR282 pKa = 11.84KK283 pKa = 9.36FDD285 pKa = 4.62YY286 pKa = 10.85IFATAKK292 pKa = 10.62DD293 pKa = 4.47FSADD297 pKa = 2.97SDD299 pKa = 4.53SIVEE303 pKa = 4.01VKK305 pKa = 10.66AADD308 pKa = 4.02GTDD311 pKa = 3.48LTHH314 pKa = 7.03NDD316 pKa = 3.08HH317 pKa = 7.21LMYY320 pKa = 10.54QATFDD325 pKa = 3.39WRR327 pKa = 11.84AATTPTPAPAAPTVLTNPRR346 pKa = 11.84TIGSEE351 pKa = 4.16DD352 pKa = 3.02PWANAVDD359 pKa = 3.92YY360 pKa = 8.75TAGNFLGTGKK370 pKa = 10.79ADD372 pKa = 3.38MVARR376 pKa = 11.84YY377 pKa = 10.04ADD379 pKa = 4.15GTVMLYY385 pKa = 9.87PGKK388 pKa = 10.73GGGAFGSGQQLEE400 pKa = 4.68LATQYY405 pKa = 11.49GFYY408 pKa = 10.39AALGRR413 pKa = 11.84GVASVTAGDD422 pKa = 4.01FDD424 pKa = 5.83GDD426 pKa = 3.89GANDD430 pKa = 3.25GRR432 pKa = 11.84ADD434 pKa = 5.83LLVSWNNGDD443 pKa = 4.18VSLSPNNGSGGLGVSIPLLPGHH465 pKa = 6.18SSIFSKK471 pKa = 8.12MTSVGVGHH479 pKa = 6.33VLGSTDD485 pKa = 3.27TAVNDD490 pKa = 4.57AVFLTANRR498 pKa = 11.84SPIISLSPPPSSTAGPPTTASYY520 pKa = 11.36SVVAYY525 pKa = 9.29PIDD528 pKa = 3.91RR529 pKa = 11.84SEE531 pKa = 4.3SGTYY535 pKa = 9.76SAGSPVSLDD544 pKa = 3.35LGGATSLTDD553 pKa = 3.56VVDD556 pKa = 3.54MTVGDD561 pKa = 3.88FTGIGHH567 pKa = 7.4AEE569 pKa = 3.54ILLRR573 pKa = 11.84HH574 pKa = 6.0SDD576 pKa = 3.73GTLSLYY582 pKa = 10.89GDD584 pKa = 3.76NAQSDD589 pKa = 4.05FTAAPLTVVDD599 pKa = 3.88NRR601 pKa = 11.84NWAALTKK608 pKa = 10.49APTPSWQGVEE618 pKa = 4.0NLVAGDD624 pKa = 3.82FDD626 pKa = 4.16GTGKK630 pKa = 10.53DD631 pKa = 3.59GVVLQWMNEE640 pKa = 4.04YY641 pKa = 10.58QGTLVPGRR649 pKa = 11.84GQTEE653 pKa = 4.38YY654 pKa = 11.16LSSTGTAPGKK664 pKa = 10.33LFNAPVQITSGRR676 pKa = 11.84FEE678 pKa = 4.47PWTTSADD685 pKa = 3.37FAAATTTSSGDD696 pKa = 2.99TDD698 pKa = 5.71LITRR702 pKa = 11.84VQGTPAVGDD711 pKa = 3.79NPASAGQTDD720 pKa = 4.19LWTGRR725 pKa = 11.84GTGTFGEE732 pKa = 4.59TEE734 pKa = 3.73IDD736 pKa = 3.63PAVEE740 pKa = 3.79PGYY743 pKa = 10.58LDD745 pKa = 3.02WATAVTVGGFGTSGAQEE762 pKa = 4.1LFAYY766 pKa = 8.73NASGGGVYY774 pKa = 10.57ALNAGTSEE782 pKa = 4.37GPPTTTVAGLQSVDD796 pKa = 2.76WANVVEE802 pKa = 4.42VVPGTFYY809 pKa = 11.1AGPYY813 pKa = 7.98SDD815 pKa = 5.32LVIRR819 pKa = 11.84YY820 pKa = 9.36ADD822 pKa = 3.49GSVQLFQNITGQNGTSPSVPTFAAPTQVEE851 pKa = 4.45PTGSDD856 pKa = 2.64GWGDD860 pKa = 3.73AEE862 pKa = 4.81QISAGDD868 pKa = 3.57FNGDD872 pKa = 3.28GNEE875 pKa = 4.04DD876 pKa = 3.85LLVRR880 pKa = 11.84GSAGSLLLYY889 pKa = 10.08PGDD892 pKa = 4.01GAGHH896 pKa = 6.8FGGSVVIDD904 pKa = 4.06SAPAMADD911 pKa = 3.36LVSVEE916 pKa = 3.86QGTVYY921 pKa = 9.22GTAAGARR928 pKa = 11.84NIYY931 pKa = 10.02RR932 pKa = 11.84FSDD935 pKa = 3.66GEE937 pKa = 4.19AIIDD941 pKa = 3.72NGSTNLVDD949 pKa = 5.11PGTQSVNGTSASFLFHH965 pKa = 7.47SDD967 pKa = 3.74DD968 pKa = 3.65DD969 pKa = 4.07TGAIQSFSYY978 pKa = 10.69QLDD981 pKa = 3.55GAPAVSLDD989 pKa = 3.46AFHH992 pKa = 7.92DD993 pKa = 4.03EE994 pKa = 4.63VTVSLQDD1001 pKa = 3.58LTPGSHH1007 pKa = 6.33TLTVTATNSADD1018 pKa = 3.43VTSAATTYY1026 pKa = 11.23GFTDD1030 pKa = 3.94DD1031 pKa = 4.5QNTGPIHH1038 pKa = 7.05AWPLSNGTGIDD1049 pKa = 3.66TVGGADD1055 pKa = 3.86LTLSSAGTSWTTDD1068 pKa = 3.37PTHH1071 pKa = 5.88GTVLTLDD1078 pKa = 3.6GTSGYY1083 pKa = 10.52GATSGPLIDD1092 pKa = 3.78TTGSFSISAWAEE1104 pKa = 3.76PAVSSAGVIASQDD1117 pKa = 3.31GTNISGFVLYY1127 pKa = 10.84ANAGGGWTFAMAPTDD1142 pKa = 3.92SATWTADD1149 pKa = 3.54RR1150 pKa = 11.84ASTANTTDD1158 pKa = 3.77NPGSWTYY1165 pKa = 9.94LTATYY1170 pKa = 10.63NAATDD1175 pKa = 3.65TMSLYY1180 pKa = 11.33VNGAPAATGTHH1191 pKa = 4.63TTTWNATGSFIIGRR1205 pKa = 11.84DD1206 pKa = 3.82FVDD1209 pKa = 3.52SAPNGFFNGDD1219 pKa = 3.13ISNVQAFNYY1228 pKa = 8.56PLSASQVTALYY1239 pKa = 10.06DD1240 pKa = 3.48QNTPPNQQ1247 pKa = 3.26

Molecular weight: 128.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9DWG6|W9DWG6_9ACTN LamGL domain-containing protein OS=Actinospica robiniae DSM 44927 OX=479430 GN=ActroDRAFT_0206 PE=4 SV=1
MM1 pKa = 7.87ANRR4 pKa = 11.84PAAVKK9 pKa = 10.3SALKK13 pKa = 10.14SQVIEE18 pKa = 4.34TPSWGYY24 pKa = 11.01GNSGTRR30 pKa = 11.84FRR32 pKa = 11.84VFPQAGVPRR41 pKa = 11.84DD42 pKa = 3.89PFEE45 pKa = 4.9KK46 pKa = 10.53LADD49 pKa = 3.65AAAVHH54 pKa = 6.37AVTGVAAPVSLHH66 pKa = 5.85IPRR69 pKa = 11.84DD70 pKa = 3.29RR71 pKa = 11.84VADD74 pKa = 3.72YY75 pKa = 11.26GKK77 pKa = 10.56LADD80 pKa = 3.91HH81 pKa = 6.84AAGLGVRR88 pKa = 11.84VGMINSNTFQDD99 pKa = 4.06EE100 pKa = 4.14DD101 pKa = 4.12CKK103 pKa = 10.96LGSVCHH109 pKa = 6.64PDD111 pKa = 2.81VGVRR115 pKa = 11.84RR116 pKa = 11.84KK117 pKa = 7.89VTPASANGPRR127 pKa = 11.84PRR129 pKa = 11.84LGSQTGIEE137 pKa = 4.0PQLRR141 pKa = 11.84RR142 pKa = 11.84DD143 pKa = 3.79KK144 pKa = 11.31NAGSHH149 pKa = 6.38PGCHH153 pKa = 5.65GADD156 pKa = 3.16RR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84AALHH163 pKa = 5.12VPAWSRR169 pKa = 11.84RR170 pKa = 11.84SS171 pKa = 3.32

Molecular weight: 18.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

191

0

191

66481

39

2170

348.1

37.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.379 ± 0.253

1.012 ± 0.05

5.614 ± 0.12

5.027 ± 0.201

2.736 ± 0.097

9.352 ± 0.16

2.156 ± 0.091

3.571 ± 0.101

1.602 ± 0.086

10.132 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.641 ± 0.064

2.127 ± 0.164

5.761 ± 0.118

3.066 ± 0.1

7.387 ± 0.34

6.121 ± 0.227

6.516 ± 0.388

7.753 ± 0.152

1.652 ± 0.063

2.398 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski