Photobacterium aquimaris
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3753 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y6KZ00|A0A1Y6KZ00_9GAMM Uncharacterized protein OS=Photobacterium aquimaris OX=512643 GN=PAQU9191_01538 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.44 KK3 pKa = 10.16 NISSICCKK11 pKa = 10.45 LFILITIYY19 pKa = 10.84 LFSTVLHH26 pKa = 6.39 AQQCSSGISVSNGEE40 pKa = 4.28 YY41 pKa = 10.89 YY42 pKa = 10.61 FDD44 pKa = 6.05 GDD46 pKa = 4.33 DD47 pKa = 4.2 QPGTTGWGARR57 pKa = 11.84 NLTVNPSGLTGGTGTGGRR75 pKa = 11.84 IIVNSTWGWNNGNQWTNRR93 pKa = 11.84 NDD95 pKa = 2.93 ATEE98 pKa = 5.01 AINITLTINGVTYY111 pKa = 11.02 ARR113 pKa = 11.84 LTTPSNSQNTATLSALNGATFTSGGGNVNVNPFGQYY149 pKa = 6.7 THH151 pKa = 6.76 NAQLLLPNSVTTIFSVQIAGTEE173 pKa = 4.24 TPFQGSGGFLGIGAQAAAGDD193 pKa = 4.04 DD194 pKa = 3.34 GGIRR198 pKa = 11.84 VNSILQCGFEE208 pKa = 4.24 ATDD211 pKa = 3.64 APSSYY216 pKa = 10.92 GIARR220 pKa = 11.84 HH221 pKa = 5.61 NRR223 pKa = 11.84 DD224 pKa = 4.04 LIANNLRR231 pKa = 11.84 LGANVSYY238 pKa = 10.82 EE239 pKa = 4.13 SANLSSTLADD249 pKa = 3.8 RR250 pKa = 11.84 DD251 pKa = 3.95 SFDD254 pKa = 4.89 DD255 pKa = 5.24 GISGFPQNMIDD266 pKa = 4.04 PVTGAISLTAMVTNGTGSSSQLAGWIDD293 pKa = 3.5 WDD295 pKa = 4.06 QDD297 pKa = 3.3 NAFQADD303 pKa = 3.85 EE304 pKa = 5.34 GITITVPAAGSSNAEE319 pKa = 4.16 CTNTGGNDD327 pKa = 3.98 FSCTLNFIVATADD340 pKa = 3.71 RR341 pKa = 11.84 TNSGYY346 pKa = 10.16 FYY348 pKa = 11.23 SRR350 pKa = 11.84 FRR352 pKa = 11.84 LSTASLTTSTISTTVTGGEE371 pKa = 3.99 VEE373 pKa = 5.07 DD374 pKa = 3.84 YY375 pKa = 10.12 RR376 pKa = 11.84 FCIGCFDD383 pKa = 3.36 ISGSIFIDD391 pKa = 3.41 EE392 pKa = 4.78 NGDD395 pKa = 3.9 SNVTGDD401 pKa = 4.73 SLTTHH406 pKa = 5.93 QPWVHH411 pKa = 7.1 LYY413 pKa = 10.53 RR414 pKa = 11.84 DD415 pKa = 4.18 DD416 pKa = 6.02 DD417 pKa = 4.32 NDD419 pKa = 4.09 GVPSAGDD426 pKa = 3.29 VLVTQQLAINGSYY439 pKa = 10.92 SFTTLPLATYY449 pKa = 8.44 FVAVAPPKK457 pKa = 10.67 LSGALAEE464 pKa = 4.16 QTYY467 pKa = 10.37 AASNTYY473 pKa = 11.28 ANAFCDD479 pKa = 3.88 ANGDD483 pKa = 3.95 GAVGDD488 pKa = 4.17 TPRR491 pKa = 11.84 TTSGACYY498 pKa = 10.18 SGIDD502 pKa = 3.97 GDD504 pKa = 4.67 RR505 pKa = 11.84 ADD507 pKa = 3.89 ATSNSTTRR515 pKa = 11.84 EE516 pKa = 4.12 HH517 pKa = 5.79 ITKK520 pKa = 9.9 VDD522 pKa = 3.54 LSFASGNQANVDD534 pKa = 4.0 FGFSYY539 pKa = 11.17 NVVTNTDD546 pKa = 2.91 NSGQGSFYY554 pKa = 10.96 QFIRR558 pKa = 11.84 NANTLSGANSMRR570 pKa = 11.84 FVPTVPANDD579 pKa = 3.84 SDD581 pKa = 5.26 GSADD585 pKa = 2.83 WWVITPTSNLTTITGTNGAEE605 pKa = 3.99 TTIDD609 pKa = 3.14 GTAYY613 pKa = 10.08 SHH615 pKa = 7.32 SDD617 pKa = 2.7 GTTVVDD623 pKa = 4.79 ANPGNYY629 pKa = 9.54 SEE631 pKa = 4.24 IQTVGSSDD639 pKa = 3.43 GCSVEE644 pKa = 4.68 TIPALAKK651 pKa = 10.43 PEE653 pKa = 4.02 LQIDD657 pKa = 3.88 RR658 pKa = 11.84 PVNSSAYY665 pKa = 9.24 NSEE668 pKa = 4.14 LLVVNADD675 pKa = 3.15 NTTVRR680 pKa = 11.84 NVSLTGGSLGINVYY694 pKa = 10.55 SGGISGSLFEE704 pKa = 5.15 NNLIGIDD711 pKa = 3.46 PAGNDD716 pKa = 3.59 KK717 pKa = 11.3 VIGQDD722 pKa = 3.0 NCGTSTGCAGIAIANPSNASLISTTGIIQNNAIKK756 pKa = 8.96 TAHH759 pKa = 6.08 NNISFGNLNGQTSTDD774 pKa = 2.94 HH775 pKa = 6.71 WLVKK779 pKa = 10.29 HH780 pKa = 5.64 NQLLGTTSTSISYY793 pKa = 9.83 DD794 pKa = 3.03 YY795 pKa = 11.0 HH796 pKa = 7.91 NIYY799 pKa = 9.64 IRR801 pKa = 11.84 YY802 pKa = 8.4 GIPAYY807 pKa = 10.76 LDD809 pKa = 3.4 VIGNILRR816 pKa = 11.84 DD817 pKa = 3.67 ATGDD821 pKa = 3.67 GIYY824 pKa = 10.29 QLGTSNVALNQTFTSNDD841 pKa = 3.1 IQHH844 pKa = 6.76 AAAAGIHH851 pKa = 5.34 MTSGHH856 pKa = 5.72 TDD858 pKa = 3.33 IITCNVIHH866 pKa = 6.69 NNGGAGVAIDD876 pKa = 4.14 GNTNVDD882 pKa = 3.34 GYY884 pKa = 11.18 LITKK888 pKa = 10.22 NSFSGNTLNAIDD900 pKa = 4.25 LHH902 pKa = 7.27 RR903 pKa = 11.84 GTTGQGVSLNSDD915 pKa = 3.6 DD916 pKa = 5.32 CNTDD920 pKa = 2.82 GTGGANNNLARR931 pKa = 11.84 PEE933 pKa = 3.59 IDD935 pKa = 3.44 YY936 pKa = 11.52 AFLTGSSLRR945 pKa = 11.84 VIGNYY950 pKa = 10.11 CGTGTFTLEE959 pKa = 3.56 IYY961 pKa = 9.97 KK962 pKa = 10.44 ASSGIGDD969 pKa = 3.72 TGSDD973 pKa = 3.33 GKK975 pKa = 10.7 DD976 pKa = 2.9 AGEE979 pKa = 4.02 GLLYY983 pKa = 10.59 LGEE986 pKa = 4.3 VMGLTGGSLDD996 pKa = 3.43 QTLTVSGLAVGDD1008 pKa = 4.76 DD1009 pKa = 3.23 ITVIVHH1015 pKa = 5.69 RR1016 pKa = 11.84 TNDD1019 pKa = 3.23 GGLGVVQDD1027 pKa = 3.86 TSEE1030 pKa = 4.25 FSANTLVDD1038 pKa = 3.9 TLNKK1042 pKa = 10.34 DD1043 pKa = 2.83 WGDD1046 pKa = 3.91 APDD1049 pKa = 4.07 SSSGTDD1055 pKa = 3.16 VNDD1058 pKa = 3.46 YY1059 pKa = 8.39 QTLRR1063 pKa = 11.84 EE1064 pKa = 4.15 NNGASHH1070 pKa = 7.51 VIKK1073 pKa = 10.62 DD1074 pKa = 4.17 SNNDD1078 pKa = 3.33 DD1079 pKa = 3.76 TPDD1082 pKa = 3.47 VVLGAIVDD1090 pKa = 3.66 ADD1092 pKa = 3.32 FDD1094 pKa = 4.48 GFNTLNALGDD1104 pKa = 3.92 NNDD1107 pKa = 3.46 NSNDD1111 pKa = 3.6 EE1112 pKa = 4.41 EE1113 pKa = 4.55 GTVAEE1118 pKa = 5.06 GYY1120 pKa = 9.98 YY1121 pKa = 10.39 VINRR1125 pKa = 11.84 SKK1127 pKa = 9.68 TISVTITKK1135 pKa = 10.45 DD1136 pKa = 3.12 PTLATATFHH1145 pKa = 6.01 LHH1147 pKa = 6.66 GWFDD1151 pKa = 3.65 WNRR1154 pKa = 11.84 DD1155 pKa = 3.54 GDD1157 pKa = 3.81 WLDD1160 pKa = 3.94 ANEE1163 pKa = 4.08 HH1164 pKa = 6.22 VINEE1168 pKa = 4.29 SAVAPGTNDD1177 pKa = 2.9 YY1178 pKa = 9.64 TFIVPQTAVLGVTYY1192 pKa = 10.78 ARR1194 pKa = 11.84 FRR1196 pKa = 11.84 VCSIGDD1202 pKa = 4.0 CNTPTGPSIDD1212 pKa = 4.3 GEE1214 pKa = 4.44 VEE1216 pKa = 4.09 DD1217 pKa = 5.08 YY1218 pKa = 11.6 SDD1220 pKa = 4.2 INISLDD1226 pKa = 3.88 FGDD1229 pKa = 5.33 APDD1232 pKa = 3.76 NGIGVGPNDD1241 pKa = 3.64 YY1242 pKa = 8.52 RR1243 pKa = 11.84 TLEE1246 pKa = 4.08 IDD1248 pKa = 3.62 DD1249 pKa = 4.47 GPRR1252 pKa = 11.84 HH1253 pKa = 5.5 EE1254 pKa = 4.8 TTDD1257 pKa = 3.66 DD1258 pKa = 3.94 LYY1260 pKa = 11.5 LGSTVDD1266 pKa = 3.48 TEE1268 pKa = 4.74 INGLQNLTATGDD1280 pKa = 3.13 NSTNVNDD1287 pKa = 4.12 EE1288 pKa = 4.55 DD1289 pKa = 4.73 SLTLAPLTTLTTTYY1303 pKa = 10.8 NATAVTTNTTGSDD1316 pKa = 3.17 AYY1318 pKa = 11.04 VYY1320 pKa = 11.08 AWLDD1324 pKa = 3.2 LDD1326 pKa = 3.98 RR1327 pKa = 11.84 NGRR1330 pKa = 11.84 FDD1332 pKa = 4.6 RR1333 pKa = 11.84 DD1334 pKa = 3.43 EE1335 pKa = 4.29 LVSNGAGTGGAVVVANGEE1353 pKa = 4.23 TSKK1356 pKa = 9.75 TLTWSGISGLTNNTALYY1373 pKa = 9.97 LRR1375 pKa = 11.84 VRR1377 pKa = 11.84 IVDD1380 pKa = 3.63 TLLTDD1385 pKa = 3.87 TATGSDD1391 pKa = 3.73 EE1392 pKa = 4.21 DD1393 pKa = 4.05 PRR1395 pKa = 11.84 SFGIQTSGEE1404 pKa = 4.14 VEE1406 pKa = 4.23 DD1407 pKa = 4.96 YY1408 pKa = 10.48 RR1409 pKa = 11.84 LQVADD1414 pKa = 4.4 QDD1416 pKa = 4.51 FGDD1419 pKa = 4.66 LNDD1422 pKa = 4.14 SFTTLALSGGPYY1434 pKa = 9.84 HH1435 pKa = 7.45 GLGNINNLHH1444 pKa = 6.44 IGSNNIDD1451 pKa = 3.77 TEE1453 pKa = 4.48 ADD1455 pKa = 3.72 GQPSAGANGDD1465 pKa = 3.88 DD1466 pKa = 4.83 DD1467 pKa = 6.0 NNANDD1472 pKa = 3.72 EE1473 pKa = 4.27 NAFDD1477 pKa = 3.92 VTPAPILALTASEE1490 pKa = 4.26 YY1491 pKa = 11.01 VITVPVRR1498 pKa = 11.84 NNSGGDD1504 pKa = 3.53 VNLSAWLDD1512 pKa = 3.32 WDD1514 pKa = 3.94 NNGQFEE1520 pKa = 4.49 AEE1522 pKa = 4.31 EE1523 pKa = 4.34 YY1524 pKa = 9.06 ATTVVPNGTVVGNPVTLTFTGITAANVSTVALRR1557 pKa = 11.84 LRR1559 pKa = 11.84 LSTDD1563 pKa = 2.96 SLANTAWGGGASDD1576 pKa = 5.19 GEE1578 pKa = 4.6 VEE1580 pKa = 4.13 DD1581 pKa = 5.19 HH1582 pKa = 6.75 YY1583 pKa = 11.56 IPVGDD1588 pKa = 4.25 FDD1590 pKa = 6.1 FGDD1593 pKa = 3.33 AHH1595 pKa = 6.61 DD1596 pKa = 5.79 AIVGSAGGSSDD1607 pKa = 3.35 YY1608 pKa = 10.7 RR1609 pKa = 11.84 SRR1611 pKa = 11.84 LVDD1614 pKa = 3.31 NGPYY1618 pKa = 10.04 HH1619 pKa = 7.37 GIANTLYY1626 pKa = 10.35 LGQAAPDD1633 pKa = 4.2 ADD1635 pKa = 4.0 PDD1637 pKa = 3.85 ARR1639 pKa = 11.84 PSTGTSYY1646 pKa = 11.91 ANGDD1650 pKa = 3.97 DD1651 pKa = 3.59 DD1652 pKa = 5.06 TEE1654 pKa = 5.87 RR1655 pKa = 11.84 DD1656 pKa = 4.09 DD1657 pKa = 3.99 EE1658 pKa = 5.55 DD1659 pKa = 3.74 GVNFVPLSLTHH1670 pKa = 5.81 PTYY1673 pKa = 10.06 TARR1676 pKa = 11.84 ATVNNTTGTTATIHH1690 pKa = 6.55 AWIDD1694 pKa = 2.98 WDD1696 pKa = 3.88 QNGQFEE1702 pKa = 4.33 QDD1704 pKa = 3.61 EE1705 pKa = 4.58 YY1706 pKa = 11.13 TSASVANNTTGTVDD1720 pKa = 4.25 LVWTTFTGRR1729 pKa = 11.84 ITGWTVSRR1737 pKa = 11.84 VRR1739 pKa = 11.84 ITTDD1743 pKa = 2.61 NLTNSAGATNLEE1755 pKa = 4.39 DD1756 pKa = 3.68 TRR1758 pKa = 11.84 SLGLASNGEE1767 pKa = 4.11 VEE1769 pKa = 4.94 DD1770 pKa = 3.67 YY1771 pKa = 10.33 RR1772 pKa = 11.84 VYY1774 pKa = 10.53 IGEE1777 pKa = 4.96 HH1778 pKa = 6.05 DD1779 pKa = 3.96 TGDD1782 pKa = 3.7 APNSYY1787 pKa = 8.62 LTLTLDD1793 pKa = 4.68 GGPCHH1798 pKa = 6.89 ALTATNSLYY1807 pKa = 10.9 LGSTLFDD1814 pKa = 4.06 NNLDD1818 pKa = 3.91 GQPTANAMGDD1828 pKa = 4.21 DD1829 pKa = 3.65 IDD1831 pKa = 5.52 GVDD1834 pKa = 4.81 DD1835 pKa = 4.23 EE1836 pKa = 6.55 DD1837 pKa = 6.59 GIADD1841 pKa = 4.29 NLPLLLTSSATYY1853 pKa = 10.2 SINVKK1858 pKa = 10.07 HH1859 pKa = 6.23 NNSSGSAATLIAWLDD1874 pKa = 3.55 KK1875 pKa = 10.77 NQNNTFEE1882 pKa = 4.71 ADD1884 pKa = 3.41 EE1885 pKa = 4.41 VFDD1888 pKa = 4.32 GFSVNNVANNTNALTTGQTLTWSGLSGQTQGTMALRR1924 pKa = 11.84 IRR1926 pKa = 11.84 YY1927 pKa = 6.23 STKK1930 pKa = 10.49 AFSANDD1936 pKa = 3.56 WGGPAPDD1943 pKa = 5.0 GEE1945 pKa = 4.62 VEE1947 pKa = 4.24 DD1948 pKa = 4.3 YY1949 pKa = 10.26 MVFVGRR1955 pKa = 11.84 FDD1957 pKa = 4.88 FGDD1960 pKa = 3.9 ASDD1963 pKa = 4.72 DD1964 pKa = 3.68 ATGATSTNNYY1974 pKa = 6.41 RR1975 pKa = 11.84 TRR1977 pKa = 11.84 LADD1980 pKa = 3.43 NGPYY1984 pKa = 10.11 HH1985 pKa = 7.56 GINNDD1990 pKa = 3.32 LYY1992 pKa = 10.59 IGLKK1996 pKa = 10.45 VPDD1999 pKa = 4.66 AEE2001 pKa = 4.59 ADD2003 pKa = 3.61 SHH2005 pKa = 6.36 NSSGTAMANGDD2016 pKa = 4.19 DD2017 pKa = 5.1 DD2018 pKa = 6.41 DD2019 pKa = 6.12 INGDD2023 pKa = 4.23 DD2024 pKa = 3.98 EE2025 pKa = 6.73 DD2026 pKa = 4.62 GVQILPLDD2034 pKa = 3.99 NSPIPASYY2042 pKa = 8.11 HH2043 pKa = 4.87 TKK2045 pKa = 10.61 VLVTNNTGSEE2055 pKa = 4.08 ATLHH2059 pKa = 6.77 GWIDD2063 pKa = 3.13 WNLNGRR2069 pKa = 11.84 FDD2071 pKa = 4.36 GDD2073 pKa = 3.34 EE2074 pKa = 4.2 HH2075 pKa = 6.4 TSLVIPNSTLAQTVDD2090 pKa = 3.85 LTWTSFTGIAEE2101 pKa = 4.5 GKK2103 pKa = 7.19 TVARR2107 pKa = 11.84 FRR2109 pKa = 11.84 LTTDD2113 pKa = 3.52 DD2114 pKa = 4.23 LTNGAATTNLEE2125 pKa = 4.33 DD2126 pKa = 3.62 TRR2128 pKa = 11.84 SLNGASDD2135 pKa = 4.32 GEE2137 pKa = 4.62 VEE2139 pKa = 4.75 DD2140 pKa = 3.32 HH2141 pKa = 6.83 HH2142 pKa = 8.9 LYY2144 pKa = 10.29 IGNQDD2149 pKa = 3.28 MGDD2152 pKa = 3.93 AEE2154 pKa = 6.17 DD2155 pKa = 4.35 SFQTLADD2162 pKa = 3.66 SLGPFHH2168 pKa = 6.67 GQSLYY2173 pKa = 10.74 TSLFLGPVPTNEE2185 pKa = 4.26 GDD2187 pKa = 4.21 ADD2189 pKa = 4.08 GQPTAAALGDD2199 pKa = 4.51 DD2200 pKa = 5.09 DD2201 pKa = 6.93 DD2202 pKa = 7.11 DD2203 pKa = 6.14 GLDD2206 pKa = 4.05 DD2207 pKa = 4.27 EE2208 pKa = 5.5 QGINQPLAFISTTDD2222 pKa = 3.08 NTFDD2226 pKa = 4.99 INVKK2230 pKa = 9.77 LQNTTTSNATLIAWLDD2246 pKa = 3.74 KK2247 pKa = 11.1 NQDD2250 pKa = 3.28 GAFTADD2256 pKa = 2.93 EE2257 pKa = 4.38 VVNTVNGSPWSVNNISSSSSYY2278 pKa = 9.11 ITDD2281 pKa = 3.46 GTAIALHH2288 pKa = 5.96 WSGLSGLRR2296 pKa = 11.84 SGVMTLRR2303 pKa = 11.84 VRR2305 pKa = 11.84 LSTDD2309 pKa = 3.17 PLTASQWGGQAIDD2322 pKa = 4.86 GEE2324 pKa = 4.55 VEE2326 pKa = 4.15 DD2327 pKa = 4.07 YY2328 pKa = 10.94 VIYY2331 pKa = 11.13 VGDD2334 pKa = 4.11 FDD2336 pKa = 6.38 FGDD2339 pKa = 4.25 ASDD2342 pKa = 4.35 SGAGGASASNYY2353 pKa = 7.43 RR2354 pKa = 11.84 TALGDD2359 pKa = 3.26 SGPYY2363 pKa = 9.95 HH2364 pKa = 7.56 GITEE2368 pKa = 4.24 KK2369 pKa = 11.09 LFMGIQAPDD2378 pKa = 3.55 AEE2380 pKa = 4.49 VDD2382 pKa = 3.51 ANTSSGVGQANGDD2395 pKa = 3.96 DD2396 pKa = 3.8 DD2397 pKa = 4.28 TDD2399 pKa = 4.93 RR2400 pKa = 11.84 DD2401 pKa = 4.26 DD2402 pKa = 4.84 EE2403 pKa = 5.94 DD2404 pKa = 3.77 GAYY2407 pKa = 10.76 LLPLDD2412 pKa = 4.91 NSPEE2416 pKa = 4.3 SNTYY2420 pKa = 8.31 VAQVTVTNEE2429 pKa = 3.61 TGQIATLHH2437 pKa = 6.09 GWVDD2441 pKa = 2.88 WDD2443 pKa = 3.77 GNGRR2447 pKa = 11.84 FDD2449 pKa = 3.66 GDD2451 pKa = 3.99 EE2452 pKa = 4.18 YY2453 pKa = 11.79 VSLPVPNGTTGDD2465 pKa = 3.86 VLEE2468 pKa = 4.78 LTWTAPYY2475 pKa = 10.16 PSIISGDD2482 pKa = 3.59 VVVRR2486 pKa = 11.84 LRR2488 pKa = 11.84 LTTDD2492 pKa = 3.23 TLINSAAASPTQEE2505 pKa = 4.38 DD2506 pKa = 4.07 TRR2508 pKa = 11.84 SLNGASNGEE2517 pKa = 4.25 VEE2519 pKa = 4.93 DD2520 pKa = 3.78 HH2521 pKa = 6.58 SLYY2524 pKa = 10.3 IGNYY2528 pKa = 9.62 DD2529 pKa = 5.02 LGDD2532 pKa = 4.1 APDD2535 pKa = 4.03 SYY2537 pKa = 11.09 LTLAISRR2544 pKa = 11.84 GPYY2547 pKa = 9.15 HH2548 pKa = 6.49 PQRR2551 pKa = 11.84 DD2552 pKa = 3.88 FSTLYY2557 pKa = 10.79 LGTIGVNEE2565 pKa = 4.77 GDD2567 pKa = 3.59 TDD2569 pKa = 4.12 GQPSTDD2575 pKa = 3.71 ARR2577 pKa = 11.84 GDD2579 pKa = 3.79 DD2580 pKa = 5.25 DD2581 pKa = 6.56 DD2582 pKa = 6.46 GLDD2585 pKa = 3.83 DD2586 pKa = 4.28 EE2587 pKa = 4.66 QAIVQPLAIVSPSITTYY2604 pKa = 10.56 SIPVRR2609 pKa = 11.84 LNNSTTDD2616 pKa = 3.53 PATVVAWLDD2625 pKa = 3.6 TNQDD2629 pKa = 3.34 GFFSADD2635 pKa = 3.32 EE2636 pKa = 4.25 VVDD2639 pKa = 4.68 TIDD2642 pKa = 3.39 GAAFAVNNVPAGTNAATNATALSFVWNGLTGLTSGQMALRR2682 pKa = 11.84 IRR2684 pKa = 11.84 ISNDD2688 pKa = 2.66 PLTAEE2693 pKa = 3.86 QWFGRR2698 pKa = 11.84 AYY2700 pKa = 10.1 NGEE2703 pKa = 4.08 VEE2705 pKa = 5.38 DD2706 pKa = 4.13 YY2707 pKa = 9.87 MVYY2710 pKa = 10.65 LGDD2713 pKa = 4.48 FDD2715 pKa = 6.31 FGDD2718 pKa = 4.41 APDD2721 pKa = 4.37 TSLSMALNDD2730 pKa = 3.97 YY2731 pKa = 7.88 QTLLANNGPRR2741 pKa = 11.84 HH2742 pKa = 6.35 LIIDD2746 pKa = 3.47 NLYY2749 pKa = 10.11 IGKK2752 pKa = 8.97 QPDD2755 pKa = 3.33 IDD2757 pKa = 5.93 DD2758 pKa = 4.19 GTLQNATATADD2769 pKa = 4.68 DD2770 pKa = 3.79 ITDD2773 pKa = 3.77 NDD2775 pKa = 4.57 DD2776 pKa = 3.26 EE2777 pKa = 6.41 DD2778 pKa = 5.8 GFTFPPLTAGMTDD2791 pKa = 3.25 YY2792 pKa = 10.39 KK2793 pKa = 10.19 ISVMITNTTGSDD2805 pKa = 3.13 ASLVTWIDD2813 pKa = 3.46 FNQNGDD2819 pKa = 3.84 FEE2821 pKa = 5.49 PDD2823 pKa = 2.75 EE2824 pKa = 4.3 GQIVIVPHH2832 pKa = 6.04 GAVDD2836 pKa = 3.61 AMGEE2840 pKa = 4.17 IEE2842 pKa = 4.07 WQNISGLANGTTLILRR2858 pKa = 11.84 SRR2860 pKa = 11.84 LLPQLVSQMSQVSAIGAEE2878 pKa = 4.04 LGGEE2882 pKa = 4.32 VEE2884 pKa = 4.18 DD2885 pKa = 4.76 HH2886 pKa = 6.98 RR2887 pKa = 11.84 INVGKK2892 pKa = 10.05 QDD2894 pKa = 3.62 MGDD2897 pKa = 3.36 APEE2900 pKa = 4.58 TYY2902 pKa = 9.28 GTDD2905 pKa = 3.52 PLNNGPHH2912 pKa = 6.27 HH2913 pKa = 7.07 LIINANDD2920 pKa = 3.85 LYY2922 pKa = 11.13 LGSNNIDD2929 pKa = 3.46 EE2930 pKa = 4.71 NTTVAASANAQGDD2943 pKa = 3.97 DD2944 pKa = 3.62 LAGVDD2949 pKa = 4.92 DD2950 pKa = 4.71 EE2951 pKa = 6.19 DD2952 pKa = 5.41 GVTEE2956 pKa = 4.33 PLLPIPTDD2964 pKa = 3.53 GSHH2967 pKa = 6.67 YY2968 pKa = 10.76 EE2969 pKa = 4.09 LILNVRR2975 pKa = 11.84 NTTGGDD2981 pKa = 3.19 AYY2983 pKa = 11.11 LVAWVDD2989 pKa = 3.73 ANRR2992 pKa = 11.84 NGQFEE2997 pKa = 4.33 PLEE3000 pKa = 4.24 GRR3002 pKa = 11.84 VDD3004 pKa = 4.07 TIINTQNGNYY3014 pKa = 9.36 TYY3016 pKa = 11.24 AFDD3019 pKa = 4.47 SNQLAYY3025 pKa = 8.28 LTPGISYY3032 pKa = 10.12 IRR3034 pKa = 11.84 FRR3036 pKa = 11.84 LSTDD3040 pKa = 3.1 PLTVTDD3046 pKa = 3.77 TGGFASDD3053 pKa = 4.84 GEE3055 pKa = 4.63 VEE3057 pKa = 4.4 DD3058 pKa = 4.23 HH3059 pKa = 6.76 LVMIGGADD3067 pKa = 4.31 LGDD3070 pKa = 4.34 LPDD3073 pKa = 4.74 ANASTTANNYY3083 pKa = 6.09 QTNLINNGPYY3093 pKa = 10.32 HH3094 pKa = 5.77 NTATLPTLYY3103 pKa = 9.88 LGNVAPDD3110 pKa = 3.95 LDD3112 pKa = 4.33 GDD3114 pKa = 4.33 SPQSNDD3120 pKa = 4.26 AQGDD3124 pKa = 4.2 DD3125 pKa = 4.48 NNGSAPDD3132 pKa = 4.64 DD3133 pKa = 3.8 EE3134 pKa = 7.08 DD3135 pKa = 4.85 GLANSVMPVLPINTSYY3151 pKa = 10.62 KK3152 pKa = 10.47 ANVVVTNDD3160 pKa = 3.05 ASRR3163 pKa = 11.84 NAYY3166 pKa = 9.89 LYY3168 pKa = 11.16 AWIDD3172 pKa = 3.19 WNRR3175 pKa = 11.84 NGDD3178 pKa = 3.77 FEE3180 pKa = 6.53 ADD3182 pKa = 4.01 EE3183 pKa = 4.28 ITQHH3187 pKa = 6.18 GFIDD3191 pKa = 3.76 STGNALAITDD3201 pKa = 4.05 GALLIPANSGDD3212 pKa = 3.26 VAYY3215 pKa = 10.83 RR3216 pKa = 11.84 LDD3218 pKa = 3.54 WNNNVATANNQTYY3231 pKa = 10.31 GLRR3234 pKa = 11.84 LRR3236 pKa = 11.84 LTTDD3240 pKa = 3.93 LLVDD3244 pKa = 4.29 DD3245 pKa = 5.0 VSTAAFDD3252 pKa = 3.92 ARR3254 pKa = 11.84 SLGAANDD3261 pKa = 4.49 GEE3263 pKa = 4.42 VEE3265 pKa = 5.08 DD3266 pKa = 4.86 YY3267 pKa = 11.09 FLTAKK3272 pKa = 10.71 NSDD3275 pKa = 3.9 LGDD3278 pKa = 3.86 AADD3281 pKa = 4.49 SYY3283 pKa = 10.12 QTSTGRR3289 pKa = 11.84 NGPLHH3294 pKa = 7.0 AYY3296 pKa = 9.6 VSNLYY3301 pKa = 10.59 LGTSNIDD3308 pKa = 3.03 NDD3310 pKa = 4.31 PNVTPSLASNSDD3322 pKa = 3.66 DD3323 pKa = 3.48 EE3324 pKa = 4.87 TNADD3328 pKa = 4.46 DD3329 pKa = 4.86 EE3330 pKa = 4.86 NGLIQPLSFIATTATTYY3347 pKa = 10.9 SVDD3350 pKa = 3.3 VAVYY3354 pKa = 8.85 NQSGSAATLVAWLDD3368 pKa = 3.73 KK3369 pKa = 11.04 NQDD3372 pKa = 3.38 GAFSTDD3378 pKa = 3.16 EE3379 pKa = 4.16 VVDD3382 pKa = 4.21 DD3383 pKa = 5.07 LNVASGGVPFSNNPFGGDD3401 pKa = 3.55 NYY3403 pKa = 10.7 PSGNDD3408 pKa = 2.89 NRR3410 pKa = 11.84 TNKK3413 pKa = 8.0 VTLQWNGLTGLTQGTMTLRR3432 pKa = 11.84 IRR3434 pKa = 11.84 VANTTLTEE3442 pKa = 4.15 SDD3444 pKa = 3.09 WFGFAAGGEE3453 pKa = 4.36 VEE3455 pKa = 5.62 DD3456 pKa = 3.76 YY3457 pKa = 9.67 TVYY3460 pKa = 10.7 VGSYY3464 pKa = 10.84 DD3465 pKa = 4.04 FGDD3468 pKa = 4.44 AEE3470 pKa = 5.01 DD3471 pKa = 4.62 GQAGTATGAGDD3482 pKa = 3.61 YY3483 pKa = 7.62 RR3484 pKa = 11.84 TTFNDD3489 pKa = 2.99 EE3490 pKa = 4.05 GPYY3493 pKa = 10.35 HH3494 pKa = 7.2 GISTDD3499 pKa = 3.45 LFMGTNATDD3508 pKa = 3.82 AEE3510 pKa = 4.16 ADD3512 pKa = 4.02 AYY3514 pKa = 10.06 PSSATAKK3521 pKa = 10.76 ADD3523 pKa = 3.46 GDD3525 pKa = 4.28 DD3526 pKa = 5.01 DD3527 pKa = 4.96 NGSTPDD3533 pKa = 4.71 DD3534 pKa = 3.68 EE3535 pKa = 5.03 DD3536 pKa = 4.47 AVIFTALDD3544 pKa = 3.6 NSPKK3548 pKa = 9.48 PSSYY3552 pKa = 9.45 STTVKK3557 pKa = 9.15 VTNNTANQANLYY3569 pKa = 9.84 GWIDD3573 pKa = 3.73 FDD3575 pKa = 5.16 RR3576 pKa = 11.84 NGRR3579 pKa = 11.84 FDD3581 pKa = 4.25 ADD3583 pKa = 3.57 EE3584 pKa = 4.3 VATLAIPASTIAADD3598 pKa = 4.1 ITLTWATIPNISAGDD3613 pKa = 3.48 TLTRR3617 pKa = 11.84 FRR3619 pKa = 11.84 LTTDD3623 pKa = 3.49 TLVHH3627 pKa = 6.42 TGAATDD3633 pKa = 3.53 EE3634 pKa = 4.31 DD3635 pKa = 4.23 HH3636 pKa = 7.44 RR3637 pKa = 11.84 SLGGASDD3644 pKa = 4.66 GEE3646 pKa = 4.59 VEE3648 pKa = 4.76 DD3649 pKa = 4.16 HH3650 pKa = 6.71 EE3651 pKa = 5.65 LYY3653 pKa = 10.34 IGNYY3657 pKa = 9.76 DD3658 pKa = 3.76 GGDD3661 pKa = 3.51 APEE3664 pKa = 4.87 SYY3666 pKa = 8.7 QTSPATGGPAHH3677 pKa = 7.7 RR3678 pKa = 11.84 YY3679 pKa = 6.65 NTQLYY3684 pKa = 10.27 LGTNNIDD3691 pKa = 3.22 AEE3693 pKa = 4.31 EE3694 pKa = 4.19 AVGSIGADD3702 pKa = 3.2 NDD3704 pKa = 4.18 DD3705 pKa = 4.46 NSGDD3709 pKa = 4.11 DD3710 pKa = 3.81 EE3711 pKa = 5.33 NGLTFSTANDD3721 pKa = 3.77 GDD3723 pKa = 3.76 SSYY3726 pKa = 11.64 QLTADD3731 pKa = 3.81 VYY3733 pKa = 10.82 NNTGADD3739 pKa = 3.2 AKK3741 pKa = 10.87 LVVWVDD3747 pKa = 2.89 WDD3749 pKa = 3.56 RR3750 pKa = 11.84 SGTFDD3755 pKa = 3.2 VGEE3758 pKa = 4.06 ATVVDD3763 pKa = 4.31 NLPSNATVTPTTVNWSGISPTLTPGDD3789 pKa = 3.91 YY3790 pKa = 10.31 YY3791 pKa = 11.55 VRR3793 pKa = 11.84 ARR3795 pKa = 11.84 LMASTEE3801 pKa = 4.53 PIDD3804 pKa = 3.55 GTTPGGIANSGEE3816 pKa = 4.25 VEE3818 pKa = 3.89 DD3819 pKa = 5.74 HH3820 pKa = 6.37 IFNVKK3825 pKa = 8.25 YY3826 pKa = 6.66 TTEE3829 pKa = 4.5 IISGLCEE3836 pKa = 3.69 NANPILAAQGGFDD3849 pKa = 3.58 VEE3851 pKa = 4.42 QFEE3854 pKa = 5.06 HH3855 pKa = 6.61 VGTTTSIDD3863 pKa = 3.6 WVTHH3867 pKa = 5.97 NYY3869 pKa = 10.08 LSSEE3873 pKa = 4.47 RR3874 pKa = 11.84 IVSAKK3879 pKa = 10.3 SVLRR3883 pKa = 11.84 DD3884 pKa = 3.3 GTLGIKK3890 pKa = 10.02 FLSNAANHH3898 pKa = 6.47 IEE3900 pKa = 4.24 LGINQPSYY3908 pKa = 11.24 TNNLYY3913 pKa = 10.58 TNVQFYY3919 pKa = 11.17 NGYY3922 pKa = 9.77 DD3923 pKa = 3.19 ASNNPNANVTRR3934 pKa = 11.84 YY3935 pKa = 9.71 RR3936 pKa = 11.84 LVMPEE3941 pKa = 4.77 DD3942 pKa = 3.46 GTITVNSLKK3951 pKa = 10.94 SGDD3954 pKa = 3.69 EE3955 pKa = 3.92 DD3956 pKa = 3.59 AVLVISGVTTLGPILYY3972 pKa = 9.6 YY3973 pKa = 10.77 GSNRR3977 pKa = 11.84 TADD3980 pKa = 3.47 LSGTFTFNQNDD3991 pKa = 4.44 TIDD3994 pKa = 4.39 FIYY3997 pKa = 10.86 RR3998 pKa = 11.84 NTTPTNYY4005 pKa = 9.58 MGTADD4010 pKa = 3.71 FTIKK4014 pKa = 10.28 FNCKK4018 pKa = 9.41 LDD4020 pKa = 3.85 YY4021 pKa = 11.34 GDD4023 pKa = 5.38 APTGYY4028 pKa = 9.2 PVLDD4032 pKa = 4.38 ADD4034 pKa = 4.1 DD4035 pKa = 4.44 GARR4038 pKa = 11.84 HH4039 pKa = 5.63 VIEE4042 pKa = 4.39 GLEE4045 pKa = 4.01 GEE4047 pKa = 4.61 IDD4049 pKa = 3.96 NLTLGNTKK4057 pKa = 10.62 DD4058 pKa = 3.58 IDD4060 pKa = 4.32 LDD4062 pKa = 3.8 GQPEE4066 pKa = 4.25 VNALGDD4072 pKa = 4.11 DD4073 pKa = 3.9 NDD4075 pKa = 4.86 AEE4077 pKa = 5.37 GDD4079 pKa = 3.95 DD4080 pKa = 3.98 EE4081 pKa = 4.68 TLVIPANITAGSAEE4095 pKa = 4.26 SIDD4098 pKa = 3.65 FVVAGGSGFINVWIDD4113 pKa = 3.09 ADD4115 pKa = 3.49 IDD4117 pKa = 3.72 NSFTTDD4123 pKa = 3.48 GEE4125 pKa = 4.5 HH4126 pKa = 6.3 VLQDD4130 pKa = 3.36 VAVAAGSNPLDD4141 pKa = 3.74 VIAPYY4146 pKa = 10.37 LGSSGNTFMRR4156 pKa = 11.84 VRR4158 pKa = 11.84 LCSIANHH4165 pKa = 6.53 CNTPTGLAGSGEE4177 pKa = 4.23 VEE4179 pKa = 4.19 DD4180 pKa = 4.26 YY4181 pKa = 11.1 QFNLLPAEE4189 pKa = 4.27 NGFGIANCDD4198 pKa = 2.93 NHH4200 pKa = 8.34 AEE4202 pKa = 4.11 LTQVWWVNGAPRR4214 pKa = 11.84 TIIDD4218 pKa = 3.69 FTNLSSGYY4226 pKa = 8.11 PTITNANGTNMATGNGGTEE4245 pKa = 4.08 GTVTITDD4252 pKa = 4.01 PLNGEE4257 pKa = 4.33 VIVYY4261 pKa = 10.52 GDD4263 pKa = 3.36 GRR4265 pKa = 11.84 GVFNGSTNTAIALPFTTGASADD4287 pKa = 3.77 FPITVTPRR4295 pKa = 11.84 PGNDD4299 pKa = 2.74 LNQFYY4304 pKa = 11.36 VFGNNFNTINAGDD4317 pKa = 4.34 LDD4319 pKa = 4.62 FGTSSIINRR4328 pKa = 11.84 GAIQTNSALEE4338 pKa = 4.08 SQLVVPHH4345 pKa = 6.37 GNRR4348 pKa = 11.84 NDD4350 pKa = 3.21 SWLLTMTRR4358 pKa = 11.84 NGQLQAFPLTAGGIGFTPVISTIPNGSGPSTNKK4391 pKa = 10.3 GAMDD4395 pKa = 3.94 YY4396 pKa = 11.34 SPVTGKK4402 pKa = 10.39 LAIARR4407 pKa = 11.84 DD4408 pKa = 4.25 GISRR4412 pKa = 11.84 LFIGDD4417 pKa = 4.19 FNPNTGEE4424 pKa = 4.27 FINVTSVTTGIARR4437 pKa = 11.84 VGSSPRR4443 pKa = 11.84 FSPDD4447 pKa = 2.32 GKK4449 pKa = 10.49 RR4450 pKa = 11.84 VFFEE4454 pKa = 4.34 NGSGGDD4460 pKa = 3.87 NGPLTYY4466 pKa = 10.73 YY4467 pKa = 10.82 DD4468 pKa = 4.18 FDD4470 pKa = 5.8 SDD4472 pKa = 3.8 TVTAVTGMPGNVQSIKK4488 pKa = 10.41 FGPDD4492 pKa = 2.36 GRR4494 pKa = 11.84 LYY4496 pKa = 10.94 AWRR4499 pKa = 11.84 GTNLDD4504 pKa = 4.13 VIEE4507 pKa = 4.73 NWDD4510 pKa = 3.62 TTPTYY4515 pKa = 7.58 TTSLTMPGSIGVEE4528 pKa = 4.13 SLPEE4532 pKa = 3.74 VYY4534 pKa = 10.08 AYY4536 pKa = 10.76 CNYY4539 pKa = 10.38 VGGTVTINDD4548 pKa = 3.82 FGDD4551 pKa = 4.11 APDD4554 pKa = 4.28 LTTQTEE4560 pKa = 4.09 QGDD4563 pKa = 4.12 YY4564 pKa = 9.49 RR4565 pKa = 11.84 TTAVNNGAEE4574 pKa = 4.46 HH4575 pKa = 7.09 ISTFTDD4581 pKa = 3.54 VYY4583 pKa = 10.63 LGQLAPDD4590 pKa = 4.1 SDD4592 pKa = 5.19 DD4593 pKa = 3.38 GTLQNITATQDD4604 pKa = 4.05 DD4605 pKa = 4.4 INGDD4609 pKa = 3.58 ADD4611 pKa = 3.9 EE4612 pKa = 6.37 DD4613 pKa = 4.24 GLQLVPLVASATNYY4627 pKa = 9.12 QATVTVTNNSGNNAYY4642 pKa = 10.37 LYY4644 pKa = 11.16 AWIDD4648 pKa = 2.95 WDD4650 pKa = 4.56 NNGKK4654 pKa = 9.6 FDD4656 pKa = 3.99 KK4657 pKa = 11.23 DD4658 pKa = 3.37 EE4659 pKa = 5.51 AIVTNKK4665 pKa = 8.35 ITINDD4670 pKa = 4.02 GTNLATQTLTWAILPEE4686 pKa = 4.21 LTNTDD4691 pKa = 2.53 SFYY4694 pKa = 10.17 IRR4696 pKa = 11.84 VRR4698 pKa = 11.84 IMYY4701 pKa = 9.62 EE4702 pKa = 3.86 DD4703 pKa = 4.09 LADD4706 pKa = 3.76 TAIGDD4711 pKa = 3.98 AEE4713 pKa = 4.41 DD4714 pKa = 3.69 PRR4716 pKa = 11.84 SYY4718 pKa = 11.36 GSVSGGEE4725 pKa = 4.04 VEE4727 pKa = 5.57 DD4728 pKa = 3.89 YY4729 pKa = 10.83 QLVVEE4734 pKa = 4.74 TVDD4737 pKa = 5.21 FGDD4740 pKa = 4.51 APASYY4745 pKa = 8.13 DD4746 pKa = 3.5 TLNAFHH4752 pKa = 7.01 SPSSTLFLGEE4762 pKa = 3.76 HH4763 pKa = 6.31 AADD4766 pKa = 3.45 IEE4768 pKa = 4.36 ATITPDD4774 pKa = 3.73 LLAISDD4780 pKa = 4.95 DD4781 pKa = 4.55 DD4782 pKa = 4.04 NASPDD4787 pKa = 4.04 DD4788 pKa = 3.91 EE4789 pKa = 7.09 DD4790 pKa = 4.12 GVNVLLPVPLDD4801 pKa = 3.29 ATEE4804 pKa = 3.88 YY4805 pKa = 9.56 RR4806 pKa = 11.84 VVVEE4810 pKa = 4.8 ASNLSLTTDD4819 pKa = 2.92 ATLWGWIDD4827 pKa = 3.32 WDD4829 pKa = 4.13 LDD4831 pKa = 3.65 GAFDD4835 pKa = 3.79 ATEE4838 pKa = 4.11 AQQLLVTPATINGQYY4853 pKa = 9.34 TLTWNGLSGLTPGVSYY4869 pKa = 10.99 LRR4871 pKa = 11.84 LRR4873 pKa = 11.84 LTTDD4877 pKa = 3.58 PLTAADD4883 pKa = 3.56 WSGAANDD4890 pKa = 5.58 GEE4892 pKa = 4.5 IEE4894 pKa = 4.13 DD4895 pKa = 4.1 HH4896 pKa = 6.83 KK4897 pKa = 11.35 LVIGLFDD4904 pKa = 4.57 FGDD4907 pKa = 3.9 APEE4910 pKa = 4.66 SYY4912 pKa = 9.13 GTDD4915 pKa = 3.4 RR4916 pKa = 11.84 IDD4918 pKa = 3.4 LTGEE4922 pKa = 4.08 GSGPMHH4928 pKa = 7.35 PISSTIFLGTTEE4940 pKa = 4.09 TDD4942 pKa = 3.19 EE4943 pKa = 4.39 EE4944 pKa = 5.22 GEE4946 pKa = 4.39 GFVDD4950 pKa = 6.24 GIDD4953 pKa = 3.64 NYY4955 pKa = 9.5 GTAVDD4960 pKa = 5.62 DD4961 pKa = 4.59 NFAFNNDD4968 pKa = 3.39 EE4969 pKa = 4.79 DD4970 pKa = 4.74 GVTIPTIISAQPGDD4984 pKa = 3.91 TVALTIHH4991 pKa = 5.85 VQTDD4995 pKa = 3.36 VAAKK4999 pKa = 8.7 VHH5001 pKa = 5.72 GWLDD5005 pKa = 3.73 FNGNGVFDD5013 pKa = 4.52 NATEE5017 pKa = 4.01 AATAIPLTSADD5028 pKa = 4.26 DD5029 pKa = 4.09 NTDD5032 pKa = 2.92 KK5033 pKa = 11.28 ALNFTVPDD5041 pKa = 3.97 DD5042 pKa = 3.95 VKK5044 pKa = 11.27 EE5045 pKa = 4.68 GISYY5049 pKa = 10.41 LRR5051 pKa = 11.84 VRR5053 pKa = 11.84 ICADD5057 pKa = 3.26 TLACDD5062 pKa = 4.23 TPHH5065 pKa = 6.94 GLAGDD5070 pKa = 4.38 GEE5072 pKa = 4.72 VEE5074 pKa = 4.07 DD5075 pKa = 4.23 HH5076 pKa = 6.77 QVTLEE5081 pKa = 3.82 VQYY5084 pKa = 11.07 DD5085 pKa = 3.73 YY5086 pKa = 12.03 GDD5088 pKa = 3.87 APEE5091 pKa = 4.39 GTGYY5095 pKa = 7.46 QTRR5098 pKa = 11.84 FLSGGPRR5105 pKa = 11.84 HH5106 pKa = 6.4 ALGNSTISLGAVLGDD5121 pKa = 3.28 VDD5123 pKa = 4.07 TDD5125 pKa = 3.87 GFGDD5129 pKa = 3.9 GTDD5132 pKa = 3.6 TNGNATDD5139 pKa = 4.56 DD5140 pKa = 4.76 DD5141 pKa = 4.25 IKK5143 pKa = 11.18 GVSPSDD5149 pKa = 3.11 EE5150 pKa = 4.5 DD5151 pKa = 5.26 GITKK5155 pKa = 9.8 SFPYY5159 pKa = 9.97 IYY5161 pKa = 10.59 DD5162 pKa = 3.86 LGSDD5166 pKa = 3.58 LAFTVDD5172 pKa = 4.87 CNDD5175 pKa = 3.5 HH5176 pKa = 7.52 DD5177 pKa = 4.22 GTTDD5181 pKa = 3.7 LTATVYY5187 pKa = 10.99 AWVDD5191 pKa = 3.73 FNLDD5195 pKa = 3.3 GDD5197 pKa = 4.09 FTDD5200 pKa = 4.05 AGEE5203 pKa = 4.47 FTFQACTDD5211 pKa = 3.63 TSNTSMGSAALTFTGYY5227 pKa = 8.05 ATPTSGTSILRR5238 pKa = 11.84 MRR5240 pKa = 11.84 ITTDD5244 pKa = 2.64 VLTQTDD5250 pKa = 4.05 VNKK5253 pKa = 10.48 SASNGEE5259 pKa = 3.88 IEE5261 pKa = 5.49 DD5262 pKa = 4.03 FEE5264 pKa = 5.62 LYY5266 pKa = 10.33 LYY5268 pKa = 10.17 QVSEE5272 pKa = 4.55 LDD5274 pKa = 4.2 GGDD5277 pKa = 3.94 APDD5280 pKa = 4.08 SYY5282 pKa = 11.15 LVEE5285 pKa = 4.17 YY5286 pKa = 11.02 SEE5288 pKa = 5.95 DD5289 pKa = 3.68 GPAHH5293 pKa = 5.71 VFSSVMYY5300 pKa = 10.59 LGTNNVDD5307 pKa = 4.02 GEE5309 pKa = 4.25 NSAASATATSDD5320 pKa = 5.24 DD5321 pKa = 3.73 NDD5323 pKa = 3.7 ATNDD5327 pKa = 3.57 EE5328 pKa = 4.28 QGITLPQLLLGGVSYY5343 pKa = 8.9 TATAKK5348 pKa = 10.39 VFNKK5352 pKa = 9.38 TGNNAILLAWLDD5364 pKa = 3.84 SNRR5367 pKa = 11.84 NGNFEE5372 pKa = 4.01 ASEE5375 pKa = 4.72 AISPITIPTGNSITSHH5391 pKa = 5.77 PLEE5394 pKa = 4.25 WTGLSPIGYY5403 pKa = 7.62 GTYY5406 pKa = 10.12 NLRR5409 pKa = 11.84 VRR5411 pKa = 11.84 VAPEE5415 pKa = 3.77 SDD5417 pKa = 3.2 GLTSADD5423 pKa = 3.21 SGGFASNGEE5432 pKa = 4.3 VEE5434 pKa = 4.55 DD5435 pKa = 4.02 HH5436 pKa = 6.31 QLTVYY5441 pKa = 9.81 EE5442 pKa = 4.62 CTVSEE5447 pKa = 4.47 SPDD5450 pKa = 3.75 PVDD5453 pKa = 3.38 LTLGYY5458 pKa = 10.62 SSGQMPIDD5466 pKa = 3.57 AQINQGVIVQRR5477 pKa = 11.84 GSMTPPYY5484 pKa = 9.5 TDD5486 pKa = 2.54 TGIGVGVITHH5496 pKa = 6.5 PTLPFPNSDD5505 pKa = 2.12 GKK5507 pKa = 10.14 MFYY5510 pKa = 10.51 DD5511 pKa = 5.42 GYY5513 pKa = 8.14 PTSFTMVYY5521 pKa = 9.98 PDD5523 pKa = 3.89 GSPRR5527 pKa = 11.84 TAMSVAVFGDD5537 pKa = 4.56 TYY5539 pKa = 10.45 TGGGAISGVALDD5551 pKa = 3.78 VNNNVVASGSFGDD5564 pKa = 3.71 NGGPFTLTSSTTPIHH5579 pKa = 5.94 RR5580 pKa = 11.84 VIYY5583 pKa = 9.68 SAEE5586 pKa = 4.0 SAAKK5590 pKa = 10.24 DD5591 pKa = 3.55 GFRR5594 pKa = 11.84 VGLISDD5600 pKa = 4.07 CAVAVARR5607 pKa = 11.84 DD5608 pKa = 3.79 YY5609 pKa = 12.09 GDD5611 pKa = 4.68 APDD5614 pKa = 3.97 SAAGTVNTDD5623 pKa = 3.14 YY5624 pKa = 10.13 NTTKK5628 pKa = 10.65 SDD5630 pKa = 3.17 TGASHH5635 pKa = 7.63 ALYY5638 pKa = 10.77 DD5639 pKa = 4.85 SDD5641 pKa = 6.1 NDD5643 pKa = 3.9 DD5644 pKa = 4.11 QVDD5647 pKa = 3.67 ITLGTAWDD5655 pKa = 3.89 IDD5657 pKa = 4.14 DD5658 pKa = 4.24 GTLQNRR5664 pKa = 11.84 LATADD5669 pKa = 4.3 DD5670 pKa = 4.12 NQGSPNDD5677 pKa = 3.72 EE5678 pKa = 4.7 DD5679 pKa = 3.9 GVLYY5683 pKa = 10.55 AVTMKK5688 pKa = 10.55 PSDD5691 pKa = 3.55 VEE5693 pKa = 4.46 TIQITTTVDD5702 pKa = 2.6 SGTDD5706 pKa = 3.44 LSGLEE5711 pKa = 4.04 VYY5713 pKa = 10.4 AWIDD5717 pKa = 3.22 WNRR5720 pKa = 11.84 DD5721 pKa = 3.02 GDD5723 pKa = 3.77 WDD5725 pKa = 3.83 DD5726 pKa = 3.87 TNEE5729 pKa = 4.07 QVVATTTATPNATEE5743 pKa = 4.53 SYY5745 pKa = 9.99 SVTVPTDD5752 pKa = 2.86 ASLGYY5757 pKa = 8.11 TYY5759 pKa = 10.31 MRR5761 pKa = 11.84 VRR5763 pKa = 11.84 VCSNVGCNSPIGEE5776 pKa = 4.18 VSNGEE5781 pKa = 4.05 VEE5783 pKa = 4.35 DD5784 pKa = 3.77 YY5785 pKa = 10.94 RR5786 pKa = 11.84 IFVSDD5791 pKa = 4.48 LNLDD5795 pKa = 3.73 NTCDD5799 pKa = 3.38 SFLVTKK5805 pKa = 10.43 SSNSGASFEE5814 pKa = 4.31 YY5815 pKa = 10.16 TQVEE5819 pKa = 4.33 PLNSAFDD5826 pKa = 4.09 FNDD5829 pKa = 2.75 IKK5831 pKa = 10.35 TGITDD5836 pKa = 4.11 FPEE5839 pKa = 4.4 LNSLAFDD5846 pKa = 5.72 RR5847 pKa = 11.84 ITGMVYY5853 pKa = 9.19 ATYY5856 pKa = 10.48 INSNNNVAIVVTDD5869 pKa = 3.54 KK5870 pKa = 11.12 QGTSFIPRR5878 pKa = 11.84 GEE5880 pKa = 4.0 ITSDD5884 pKa = 2.79 GSYY5887 pKa = 11.07 NLSNINGSGAQSYY5900 pKa = 10.84 SVDD5903 pKa = 3.7 DD5904 pKa = 3.99 VFTNAIGSPNTGTLSTNGEE5923 pKa = 3.86 QYY5925 pKa = 11.16 YY5926 pKa = 8.33 ITNTSWNGVVIVNLADD5942 pKa = 3.35 LTFSIKK5948 pKa = 10.15 PLPTEE5953 pKa = 4.53 LLTGTQQRR5961 pKa = 11.84 IGPDD5965 pKa = 2.96 WAVSYY5970 pKa = 11.12 HH5971 pKa = 7.4 DD5972 pKa = 4.12 GLLYY5976 pKa = 10.88 GADD5979 pKa = 3.18 LTGNQNAGTPKK5990 pKa = 10.34 LYY5992 pKa = 10.63 QYY5994 pKa = 10.37 DD5995 pKa = 3.63 ISSDD5999 pKa = 3.72 TVISSDD6005 pKa = 3.77 LDD6007 pKa = 3.66 FGGRR6011 pKa = 11.84 KK6012 pKa = 8.84 PPNFSSGAVATDD6024 pKa = 3.88 DD6025 pKa = 3.81 STHH6028 pKa = 7.22 LYY6030 pKa = 10.91 LLTNGGDD6037 pKa = 3.46 HH6038 pKa = 7.02 DD6039 pKa = 4.68 TNGDD6043 pKa = 3.15 GSYY6046 pKa = 11.49 DD6047 pKa = 3.46 LFNRR6051 pKa = 11.84 IGMYY6055 pKa = 10.22 RR6056 pKa = 11.84 INLVSGFTTFVDD6068 pKa = 3.45 SGSDD6072 pKa = 3.34 VSLQFNDD6079 pKa = 3.71 AAGCLLSIDD6088 pKa = 4.42 YY6089 pKa = 10.97 GDD6091 pKa = 4.34 APIDD6095 pKa = 3.65 YY6096 pKa = 10.42 GSAGHH6101 pKa = 6.03 EE6102 pKa = 4.0 NRR6104 pKa = 11.84 DD6105 pKa = 3.47 PSMSGTPDD6113 pKa = 4.74 LILGLLWDD6121 pKa = 5.04 PDD6123 pKa = 3.43 LSDD6126 pKa = 5.01 RR6127 pKa = 11.84 YY6128 pKa = 10.69 SADD6131 pKa = 3.0 ATGDD6135 pKa = 3.91 DD6136 pKa = 5.22 LSGLDD6141 pKa = 5.06 DD6142 pKa = 4.31 EE6143 pKa = 6.32 DD6144 pKa = 4.91 GVDD6147 pKa = 3.94 MPADD6151 pKa = 4.94 LIVATATPLPITVTGMSGYY6170 pKa = 10.92 LSIFADD6176 pKa = 3.79 LDD6178 pKa = 3.79 GDD6180 pKa = 4.33 LSFSTSAAEE6189 pKa = 4.16 TVLNDD6194 pKa = 3.46 YY6195 pKa = 10.82 LITPGTTNVSILLDD6209 pKa = 3.75 AAASGGYY6216 pKa = 10.01 NGDD6219 pKa = 3.19 SFIRR6223 pKa = 11.84 FRR6225 pKa = 11.84 LCEE6228 pKa = 4.2 TEE6230 pKa = 4.26 NSCNTPTGMVDD6241 pKa = 2.85 NGEE6244 pKa = 4.27 VEE6246 pKa = 5.66 DD6247 pKa = 4.96 YY6248 pKa = 10.04 MFNLINQIVLNGTVFEE6264 pKa = 5.64 DD6265 pKa = 3.38 NGQSGGVAHH6274 pKa = 7.79 DD6275 pKa = 4.0 GLQDD6279 pKa = 3.36 GSEE6282 pKa = 3.77 RR6283 pKa = 11.84 GLANFVVKK6291 pKa = 10.62 AIYY6294 pKa = 9.97 KK6295 pKa = 7.76 GTGISGYY6302 pKa = 8.64 VTDD6305 pKa = 4.77 QLITSTVTNGAGKK6318 pKa = 8.11 YY6319 pKa = 7.58 TLVIPVEE6326 pKa = 4.19 LSDD6329 pKa = 5.27 EE6330 pKa = 4.46 EE6331 pKa = 5.07 IEE6333 pKa = 4.27 LQVVSQAAWVDD6344 pKa = 3.0 ISEE6347 pKa = 4.34 IDD6349 pKa = 3.47 TTEE6352 pKa = 3.83 VSLGLHH6358 pKa = 6.46 DD6359 pKa = 4.91 KK6360 pKa = 8.78 VTNVSNTDD6368 pKa = 2.87 SKK6370 pKa = 11.21 MLIEE6374 pKa = 4.75 ASAGDD6379 pKa = 3.91 FLEE6382 pKa = 4.56 NLDD6385 pKa = 3.87 FGKK6388 pKa = 10.93 VSVPTLEE6395 pKa = 4.45 PDD6397 pKa = 2.98 NYY6399 pKa = 10.75 TEE6401 pKa = 4.47 TEE6403 pKa = 3.91 PGLPVVFSHH6412 pKa = 6.79 KK6413 pKa = 10.66 FNVNTSGDD6421 pKa = 3.52 VSFTITNQQASPSGYY6436 pKa = 7.69 PWSEE6440 pKa = 3.54 VLYY6443 pKa = 10.76 FDD6445 pKa = 5.48 ANCNGEE6451 pKa = 3.67 IDD6453 pKa = 3.66 AGVDD6457 pKa = 3.37 GFVINPTAVSADD6469 pKa = 3.18 ATTQVCVLVKK6479 pKa = 10.64 VMVPANVPLHH6489 pKa = 6.29 AVYY6492 pKa = 10.37 NYY6494 pKa = 10.41 QLNADD6499 pKa = 3.74 MAFANTTEE6507 pKa = 4.07 TRR6509 pKa = 11.84 QVSDD6513 pKa = 2.71 VDD6515 pKa = 4.07 TIKK6518 pKa = 10.94 VSFSGAGEE6526 pKa = 4.02 LEE6528 pKa = 4.07 IEE6530 pKa = 4.3 KK6531 pKa = 9.31 TVQNITTSDD6540 pKa = 3.62 GEE6542 pKa = 4.46 SRR6544 pKa = 11.84 SNQAKK6549 pKa = 9.96 PGDD6552 pKa = 3.61 VLEE6555 pKa = 4.07 YY6556 pKa = 10.53 KK6557 pKa = 10.1 IYY6559 pKa = 10.41 FINNGSGPIDD6569 pKa = 3.71 TIKK6572 pKa = 10.91 LFDD6575 pKa = 5.75 AIPEE6579 pKa = 4.15 YY6580 pKa = 10.95 TEE6582 pKa = 3.72 LAEE6585 pKa = 4.41 VLNCTSPATLLPSSIVSCNVQAVDD6609 pKa = 3.47 DD6610 pKa = 4.42 TNAVGYY6616 pKa = 9.99 EE6617 pKa = 4.36 GGIEE6621 pKa = 3.96 WQLGGTLAPAEE6632 pKa = 4.41 SGYY6635 pKa = 8.6 VTYY6638 pKa = 10.36 RR6639 pKa = 11.84 VKK6641 pKa = 11.06 VKK6643 pKa = 10.81
Molecular weight: 703.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.669
IPC2_protein 3.694
IPC_protein 3.77
Toseland 3.528
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.427
Solomon 3.77
Lehninger 3.732
Nozaki 3.872
DTASelect 4.177
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.897
Patrickios 1.163
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A1B8HUZ9|A0A1B8HUZ9_9GAMM HlyD family secretion protein OS=Photobacterium aquimaris OX=512643 GN=emrA PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 ATINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3753
0
3753
1203080
30
6643
320.6
35.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.817 ± 0.046
1.144 ± 0.016
5.5 ± 0.049
5.238 ± 0.038
4.073 ± 0.029
6.627 ± 0.048
2.38 ± 0.023
7.092 ± 0.035
5.107 ± 0.039
10.236 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.024
4.681 ± 0.035
3.945 ± 0.021
4.945 ± 0.047
4.346 ± 0.035
6.122 ± 0.037
5.852 ± 0.045
6.766 ± 0.033
1.254 ± 0.017
3.166 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here