Thermasporomyces composti
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3739 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D9V4Q6|A0A3D9V4Q6_9ACTN Exopolyphosphatase/guanosine-5'-triphosphate 3'-diphosphate pyrophosphatase OS=Thermasporomyces composti OX=696763 GN=DFJ64_1069 PE=4 SV=1
MM1 pKa = 7.57 TYY3 pKa = 10.6 VIAQPCVDD11 pKa = 4.5 LKK13 pKa = 11.4 DD14 pKa = 3.66 RR15 pKa = 11.84 ACVDD19 pKa = 3.86 EE20 pKa = 5.5 CPVDD24 pKa = 4.39 CIYY27 pKa = 11.13 EE28 pKa = 4.32 GARR31 pKa = 11.84 MLYY34 pKa = 9.23 IHH36 pKa = 7.53 PDD38 pKa = 3.09 EE39 pKa = 5.5 CVDD42 pKa = 4.38 CGACEE47 pKa = 3.85 PVCPVEE53 pKa = 4.7 AIFYY57 pKa = 9.85 EE58 pKa = 4.69 DD59 pKa = 5.22 DD60 pKa = 4.81 VPEE63 pKa = 3.96 QWKK66 pKa = 10.38 DD67 pKa = 3.43 YY68 pKa = 8.58 YY69 pKa = 9.47 TANVEE74 pKa = 4.23 FFDD77 pKa = 5.64 DD78 pKa = 3.97 LGSPGGASKK87 pKa = 9.62 TGVINKK93 pKa = 8.01 DD94 pKa = 3.22 HH95 pKa = 7.11 PLIAALPPQNTEE107 pKa = 3.76 GG108 pKa = 3.52
Molecular weight: 11.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.908
IPC2_protein 3.923
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.846
Lehninger 3.808
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 0.108
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.892
Protein with the highest isoelectric point:
>tr|A0A3D9V9S6|A0A3D9V9S6_9ACTN ADP-heptose:LPS heptosyltransferase OS=Thermasporomyces composti OX=696763 GN=DFJ64_2345 PE=4 SV=1
MM1 pKa = 7.2 TATGANPDD9 pKa = 3.49 APALEE14 pKa = 4.25 EE15 pKa = 4.17 SRR17 pKa = 11.84 TGRR20 pKa = 11.84 EE21 pKa = 3.49 TSVWRR26 pKa = 11.84 WVGQPVAVLIMVAACLGYY44 pKa = 10.61 VAIADD49 pKa = 4.35 LDD51 pKa = 3.93 AVAARR56 pKa = 11.84 QLTWSALATRR66 pKa = 11.84 TWEE69 pKa = 4.35 HH70 pKa = 5.68 IRR72 pKa = 11.84 LTFVATLVVLAIALPCGVLLTRR94 pKa = 11.84 QRR96 pKa = 11.84 TKK98 pKa = 10.81 AAAPLVTIAANVGQAAPAIGLLVLLALWLGLGFRR132 pKa = 11.84 TSVIALSAYY141 pKa = 9.98 AVLPVLRR148 pKa = 11.84 NTIAGIRR155 pKa = 11.84 GVDD158 pKa = 3.52 PRR160 pKa = 11.84 LVEE163 pKa = 4.22 AGRR166 pKa = 11.84 GMGMSATSVLFRR178 pKa = 11.84 IEE180 pKa = 4.44 LVLALPVILAGVRR193 pKa = 11.84 VALILLVGTATLATFIDD210 pKa = 4.35 GGGLGLLITTGIKK223 pKa = 9.87 LQRR226 pKa = 11.84 TSVLVVGAILVAALALLIDD245 pKa = 3.36 WLGRR249 pKa = 11.84 VVEE252 pKa = 4.34 EE253 pKa = 3.93 LARR256 pKa = 11.84 PKK258 pKa = 10.84 GVV260 pKa = 2.97
Molecular weight: 27.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.385
IPC_protein 10.409
Toseland 10.526
ProMoST 10.365
Dawson 10.628
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.628
Grimsley 10.687
Solomon 10.818
Lehninger 10.774
Nozaki 10.555
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.95
Sillero 10.584
Patrickios 10.394
IPC_peptide 10.818
IPC2_peptide 9.692
IPC2.peptide.svr19 8.633
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3739
0
3739
1275144
29
1641
341.0
36.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.549 ± 0.048
0.777 ± 0.01
6.07 ± 0.031
5.926 ± 0.036
2.803 ± 0.021
8.695 ± 0.034
2.157 ± 0.018
3.471 ± 0.026
1.793 ± 0.025
10.301 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.645 ± 0.013
1.722 ± 0.018
6.174 ± 0.031
2.649 ± 0.02
8.879 ± 0.041
5.034 ± 0.023
5.993 ± 0.028
9.485 ± 0.038
1.643 ± 0.018
2.231 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here