Campylobacter iguaniorum
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1757 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076FDT0|A0A076FDT0_9PROT 30S ribosomal protein S4 OS=Campylobacter iguaniorum OX=1244531 GN=rpsD PE=3 SV=1
MM1 pKa = 7.63 CMYY4 pKa = 10.39 CEE6 pKa = 4.04 KK7 pKa = 10.66 PKK9 pKa = 10.81 TEE11 pKa = 4.91 DD12 pKa = 3.11 EE13 pKa = 4.45 HH14 pKa = 6.85 FLCEE18 pKa = 3.98 VCGVGMCDD26 pKa = 2.87 EE27 pKa = 5.28 CYY29 pKa = 10.4 DD30 pKa = 4.29 ALVEE34 pKa = 4.29 HH35 pKa = 7.55 DD36 pKa = 3.99 GHH38 pKa = 6.43 FHH40 pKa = 7.8 LILEE44 pKa = 4.58 NCDD47 pKa = 3.5 DD48 pKa = 3.97 EE49 pKa = 6.46 RR50 pKa = 11.84 EE51 pKa = 3.86 IDD53 pKa = 5.61 LITKK57 pKa = 9.9 ACGGSEE63 pKa = 4.01 PDD65 pKa = 5.0 YY66 pKa = 11.09 ICEE69 pKa = 4.11 DD70 pKa = 3.94 CVNKK74 pKa = 10.31 ILEE77 pKa = 4.34 NPFKK81 pKa = 11.35 DD82 pKa = 3.08 MDD84 pKa = 3.63 VSNIIEE90 pKa = 4.95 FIDD93 pKa = 3.54 NKK95 pKa = 10.79 LPDD98 pKa = 3.93 GEE100 pKa = 4.63 EE101 pKa = 3.84 IYY103 pKa = 10.67 PFSSEE108 pKa = 4.54 DD109 pKa = 3.41 YY110 pKa = 10.49 PLEE113 pKa = 3.94 FDD115 pKa = 3.34 KK116 pKa = 11.83 NEE118 pKa = 3.87 WEE120 pKa = 4.18 EE121 pKa = 4.35 VEE123 pKa = 4.54 GNPILWKK130 pKa = 9.1 TCLMDD135 pKa = 4.2 NNGDD139 pKa = 3.58 INRR142 pKa = 11.84 EE143 pKa = 4.16 TISDD147 pKa = 3.54 AFLSKK152 pKa = 10.75 RR153 pKa = 11.84 EE154 pKa = 4.02 ALMDD158 pKa = 4.06 FYY160 pKa = 11.59 KK161 pKa = 11.01 NRR163 pKa = 11.84 DD164 pKa = 3.26 EE165 pKa = 4.25 EE166 pKa = 4.31
Molecular weight: 19.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 4.012
IPC_protein 3.986
Toseland 3.795
ProMoST 4.088
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.834
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.228
Thurlkill 3.821
EMBOSS 3.846
Sillero 4.088
Patrickios 0.973
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|A0A076FA49|A0A076FA49_9PROT Tyrosine--tRNA ligase OS=Campylobacter iguaniorum OX=1244531 GN=tyrS PE=3 SV=1
MM1 pKa = 7.33 RR2 pKa = 11.84 NKK4 pKa = 10.42 YY5 pKa = 9.86 IEE7 pKa = 4.44 AFEE10 pKa = 4.1 NAQISNKK17 pKa = 9.82 SVPDD21 pKa = 4.2 FRR23 pKa = 11.84 AGDD26 pKa = 3.72 TLRR29 pKa = 11.84 IAIRR33 pKa = 11.84 IKK35 pKa = 10.55 EE36 pKa = 4.05 GDD38 pKa = 3.26 KK39 pKa = 11.19 SRR41 pKa = 11.84 VQNFEE46 pKa = 3.89 GVCIARR52 pKa = 11.84 RR53 pKa = 11.84 GTGAGEE59 pKa = 3.79 TFIIRR64 pKa = 11.84 KK65 pKa = 9.33 IGANSVGVEE74 pKa = 4.32 RR75 pKa = 11.84 IFPIYY80 pKa = 10.3 SEE82 pKa = 4.14 SLEE85 pKa = 4.3 TIEE88 pKa = 3.72 VLRR91 pKa = 11.84 RR92 pKa = 11.84 GRR94 pKa = 11.84 VRR96 pKa = 11.84 RR97 pKa = 11.84 SKK99 pKa = 10.85 LFYY102 pKa = 10.73 LRR104 pKa = 11.84 DD105 pKa = 3.63 RR106 pKa = 11.84 RR107 pKa = 11.84 GKK109 pKa = 9.22 AARR112 pKa = 11.84 IKK114 pKa = 9.86 EE115 pKa = 4.17 LKK117 pKa = 9.94 KK118 pKa = 10.87
Molecular weight: 13.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.736
IPC_protein 10.657
Toseland 10.965
ProMoST 10.804
Dawson 11.008
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 11.184
Grimsley 11.052
Solomon 11.213
Lehninger 11.169
Nozaki 10.935
DTASelect 10.745
Thurlkill 10.95
EMBOSS 11.374
Sillero 10.95
Patrickios 10.921
IPC_peptide 11.213
IPC2_peptide 9.706
IPC2.peptide.svr19 8.72
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1757
0
1757
534331
37
1505
304.1
34.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.182 ± 0.052
1.175 ± 0.023
5.71 ± 0.046
6.274 ± 0.058
5.502 ± 0.059
6.361 ± 0.048
1.457 ± 0.023
8.639 ± 0.055
8.703 ± 0.054
9.868 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.027
5.912 ± 0.053
2.894 ± 0.032
2.708 ± 0.031
3.142 ± 0.034
7.039 ± 0.047
4.502 ± 0.045
6.121 ± 0.051
0.704 ± 0.02
3.661 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here