Couchioplanes caeruleus subsp. caeruleus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Couchioplanes; Couchioplanes caeruleus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K0GUB3|A0A1K0GUB3_9ACTN Enoyl-CoA hydratase OS=Couchioplanes caeruleus subsp. caeruleus OX=56427 GN=BG844_17975 PE=4 SV=1
MM1 pKa = 7.68QEE3 pKa = 3.88EE4 pKa = 5.01PIDD7 pKa = 4.21PFNGDD12 pKa = 3.71PADD15 pKa = 4.02PAAGLDD21 pKa = 4.2DD22 pKa = 3.89LTEE25 pKa = 4.58DD26 pKa = 4.91AEE28 pKa = 4.29QDD30 pKa = 3.52PLTEE34 pKa = 4.58AEE36 pKa = 4.21RR37 pKa = 11.84QDD39 pKa = 3.78VLEE42 pKa = 5.06DD43 pKa = 3.7LSDD46 pKa = 4.04LEE48 pKa = 4.86IYY50 pKa = 9.98QALLSPTGIRR60 pKa = 11.84GLVIEE65 pKa = 5.58CEE67 pKa = 4.47DD68 pKa = 3.68CHH70 pKa = 6.56EE71 pKa = 4.0PHH73 pKa = 6.71YY74 pKa = 10.76FDD76 pKa = 4.51WDD78 pKa = 3.86LLRR81 pKa = 11.84GNLRR85 pKa = 11.84HH86 pKa = 6.15LLSSGRR92 pKa = 11.84PRR94 pKa = 11.84VHH96 pKa = 6.65EE97 pKa = 4.06PAYY100 pKa = 10.8DD101 pKa = 4.24PDD103 pKa = 4.78PDD105 pKa = 5.1HH106 pKa = 6.67YY107 pKa = 10.69VTWEE111 pKa = 3.91YY112 pKa = 11.54ARR114 pKa = 11.84GYY116 pKa = 10.77ADD118 pKa = 3.94GVHH121 pKa = 6.61DD122 pKa = 4.43TLTDD126 pKa = 3.85GSDD129 pKa = 3.79DD130 pKa = 3.89EE131 pKa = 4.67QSS133 pKa = 3.0

Molecular weight: 15.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K0GRD1|A0A1K0GRD1_9ACTN Uncharacterized protein OS=Couchioplanes caeruleus subsp. caeruleus OX=56427 GN=BG844_24145 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.19GRR40 pKa = 11.84GKK42 pKa = 10.85LSAA45 pKa = 4.32

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6851

0

6851

2182960

32

9676

318.6

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.124 ± 0.051

0.776 ± 0.008

6.077 ± 0.026

5.185 ± 0.025

2.708 ± 0.015

9.217 ± 0.033

2.223 ± 0.013

3.459 ± 0.019

1.874 ± 0.022

10.371 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.793 ± 0.011

1.776 ± 0.016

5.992 ± 0.026

2.766 ± 0.017

8.244 ± 0.032

4.893 ± 0.021

6.104 ± 0.028

8.821 ± 0.033

1.555 ± 0.012

2.042 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski