Mucilaginibacter sp. MD40

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter; unclassified Mucilaginibacter

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2RZS1|A0A2A2RZS1_9SPHI Energy-dependent translational throttle protein EttA OS=Mucilaginibacter sp. MD40 OX=2029590 GN=ettA PE=3 SV=1
MM1 pKa = 7.53EE2 pKa = 5.75GEE4 pKa = 4.26NDD6 pKa = 5.44DD7 pKa = 5.18IVDD10 pKa = 5.03DD11 pKa = 4.42EE12 pKa = 4.33NLNYY16 pKa = 10.69NEE18 pKa = 4.56ASNSYY23 pKa = 9.89EE24 pKa = 3.91YY25 pKa = 10.57DD26 pKa = 3.41VKK28 pKa = 11.24GDD30 pKa = 3.66NEE32 pKa = 5.4DD33 pKa = 3.5YY34 pKa = 11.21DD35 pKa = 5.06HH36 pKa = 7.96PDD38 pKa = 3.75PYY40 pKa = 10.95DD41 pKa = 3.49TTADD45 pKa = 3.58NGDD48 pKa = 4.73DD49 pKa = 3.73FDD51 pKa = 4.14STYY54 pKa = 11.65DD55 pKa = 3.38EE56 pKa = 4.99ANPYY60 pKa = 7.64DD61 pKa = 3.82TKK63 pKa = 11.43GEE65 pKa = 4.02YY66 pKa = 10.15DD67 pKa = 3.14AGRR70 pKa = 11.84SLEE73 pKa = 4.03TDD75 pKa = 3.19AEE77 pKa = 4.37SLGMHH82 pKa = 6.75IDD84 pKa = 3.2SGKK87 pKa = 9.91IVHH90 pKa = 7.08LDD92 pKa = 3.39PRR94 pKa = 11.84DD95 pKa = 3.44EE96 pKa = 4.7ALSHH100 pKa = 5.82TPEE103 pKa = 5.46DD104 pKa = 4.64DD105 pKa = 4.83RR106 pKa = 11.84DD107 pKa = 3.93DD108 pKa = 4.68LDD110 pKa = 4.06EE111 pKa = 4.78EE112 pKa = 5.56GYY114 pKa = 9.97PKK116 pKa = 10.31NDD118 pKa = 3.38KK119 pKa = 10.69PSII122 pKa = 3.91

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2S3P1|A0A2A2S3P1_9SPHI Uncharacterized protein OS=Mucilaginibacter sp. MD40 OX=2029590 GN=CKK33_06185 PE=4 SV=1
MM1 pKa = 7.14AVKK4 pKa = 10.33RR5 pKa = 11.84FRR7 pKa = 11.84PVTPGTRR14 pKa = 11.84FRR16 pKa = 11.84IDD18 pKa = 3.22VSNSDD23 pKa = 2.68ITTNVPEE30 pKa = 4.37KK31 pKa = 10.96SLVVANNKK39 pKa = 9.79RR40 pKa = 11.84SGGRR44 pKa = 11.84NHH46 pKa = 6.53SGKK49 pKa = 8.29MTMRR53 pKa = 11.84YY54 pKa = 9.71LGGGHH59 pKa = 5.79KK60 pKa = 9.32QAYY63 pKa = 9.25RR64 pKa = 11.84LIDD67 pKa = 3.77FKK69 pKa = 11.2RR70 pKa = 11.84NKK72 pKa = 9.79FDD74 pKa = 3.33IPAKK78 pKa = 10.04VATIEE83 pKa = 3.94YY84 pKa = 10.02DD85 pKa = 3.41PNRR88 pKa = 11.84SARR91 pKa = 11.84IALLHH96 pKa = 5.84YY97 pKa = 10.78ADD99 pKa = 3.81GEE101 pKa = 4.22KK102 pKa = 10.19RR103 pKa = 11.84YY104 pKa = 9.39MIAPEE109 pKa = 4.23GLTVGMTVVSGEE121 pKa = 4.06GVAPEE126 pKa = 4.51VGNTMPLKK134 pKa = 10.3NIPLGSIIHH143 pKa = 6.37NIEE146 pKa = 4.29LNPGQGGTIARR157 pKa = 11.84SAGTYY162 pKa = 9.45AQLSARR168 pKa = 11.84DD169 pKa = 3.59GKK171 pKa = 10.68YY172 pKa = 10.68AIIKK176 pKa = 9.48LPSGEE181 pKa = 3.92TRR183 pKa = 11.84MILSTCLATIGTVSNGEE200 pKa = 3.9KK201 pKa = 10.69ANAVLGKK208 pKa = 10.19AGRR211 pKa = 11.84KK212 pKa = 7.93RR213 pKa = 11.84WLGRR217 pKa = 11.84RR218 pKa = 11.84PRR220 pKa = 11.84VRR222 pKa = 11.84GVAMNPVDD230 pKa = 3.68HH231 pKa = 7.13PMGGGEE237 pKa = 3.89GRR239 pKa = 11.84ASGGHH244 pKa = 5.06PRR246 pKa = 11.84SRR248 pKa = 11.84KK249 pKa = 8.98GLLAKK254 pKa = 10.28GYY256 pKa = 7.47KK257 pKa = 9.2TRR259 pKa = 11.84DD260 pKa = 3.26KK261 pKa = 11.23KK262 pKa = 10.96KK263 pKa = 10.26GSDD266 pKa = 2.91RR267 pKa = 11.84YY268 pKa = 10.18IIEE271 pKa = 4.05RR272 pKa = 11.84RR273 pKa = 11.84KK274 pKa = 10.17KK275 pKa = 9.82

Molecular weight: 29.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3800

0

3800

1281753

25

2635

337.3

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.967 ± 0.039

0.787 ± 0.013

5.456 ± 0.028

5.36 ± 0.052

4.75 ± 0.029

6.857 ± 0.036

1.959 ± 0.02

7.091 ± 0.035

6.901 ± 0.041

9.401 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.021

5.637 ± 0.041

3.924 ± 0.022

3.829 ± 0.023

4.081 ± 0.025

5.957 ± 0.032

5.832 ± 0.042

6.503 ± 0.029

1.148 ± 0.016

4.193 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski