Amino acid dipepetide frequency for Mucilaginibacter sp. MD40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.22AlaAla: 7.22 ± 0.09
0.724AlaCys: 0.724 ± 0.025
5.066AlaAsp: 5.066 ± 0.069
4.495AlaGlu: 4.495 ± 0.077
3.641AlaPhe: 3.641 ± 0.054
6.222AlaGly: 6.222 ± 0.082
1.323AlaHis: 1.323 ± 0.03
5.759AlaIle: 5.759 ± 0.061
5.066AlaLys: 5.066 ± 0.068
7.425AlaLeu: 7.425 ± 0.094
1.959AlaMet: 1.959 ± 0.041
4.108AlaAsn: 4.108 ± 0.059
2.571AlaPro: 2.571 ± 0.048
3.184AlaGln: 3.184 ± 0.055
2.92AlaArg: 2.92 ± 0.054
4.413AlaSer: 4.413 ± 0.058
4.349AlaThr: 4.349 ± 0.076
5.268AlaVal: 5.268 ± 0.069
0.807AlaTrp: 0.807 ± 0.026
3.146AlaTyr: 3.146 ± 0.053
0.001AlaXaa: 0.001 ± 0.001
Cys
0.598CysAla: 0.598 ± 0.023
0.141CysCys: 0.141 ± 0.011
0.396CysAsp: 0.396 ± 0.018
0.352CysGlu: 0.352 ± 0.016
0.421CysPhe: 0.421 ± 0.02
0.641CysGly: 0.641 ± 0.025
0.2CysHis: 0.2 ± 0.013
0.609CysIle: 0.609 ± 0.021
0.491CysLys: 0.491 ± 0.02
0.758CysLeu: 0.758 ± 0.027
0.186CysMet: 0.186 ± 0.012
0.376CysAsn: 0.376 ± 0.017
0.328CysPro: 0.328 ± 0.016
0.233CysGln: 0.233 ± 0.014
0.333CysArg: 0.333 ± 0.016
0.516CysSer: 0.516 ± 0.017
0.417CysThr: 0.417 ± 0.019
0.46CysVal: 0.46 ± 0.019
0.093CysTrp: 0.093 ± 0.008
0.317CysTyr: 0.317 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.298AspAla: 4.298 ± 0.067
0.375AspCys: 0.375 ± 0.017
2.823AspAsp: 2.823 ± 0.047
3.434AspGlu: 3.434 ± 0.055
2.875AspPhe: 2.875 ± 0.059
3.757AspGly: 3.757 ± 0.066
1.093AspHis: 1.093 ± 0.034
4.165AspIle: 4.165 ± 0.068
4.043AspLys: 4.043 ± 0.067
4.81AspLeu: 4.81 ± 0.084
1.366AspMet: 1.366 ± 0.037
2.939AspAsn: 2.939 ± 0.054
2.099AspPro: 2.099 ± 0.045
1.705AspGln: 1.705 ± 0.037
2.212AspArg: 2.212 ± 0.044
2.774AspSer: 2.774 ± 0.049
2.889AspThr: 2.889 ± 0.053
3.622AspVal: 3.622 ± 0.057
0.763AspTrp: 0.763 ± 0.024
2.52AspTyr: 2.52 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
4.323GluAla: 4.323 ± 0.074
0.334GluCys: 0.334 ± 0.017
2.559GluAsp: 2.559 ± 0.056
3.303GluGlu: 3.303 ± 0.074
2.18GluPhe: 2.18 ± 0.047
3.184GluGly: 3.184 ± 0.055
1.223GluHis: 1.223 ± 0.03
3.862GluIle: 3.862 ± 0.061
4.259GluLys: 4.259 ± 0.066
5.569GluLeu: 5.569 ± 0.089
1.438GluMet: 1.438 ± 0.037
2.919GluAsn: 2.919 ± 0.051
1.717GluPro: 1.717 ± 0.039
2.577GluGln: 2.577 ± 0.049
2.601GluArg: 2.601 ± 0.059
2.529GluSer: 2.529 ± 0.044
2.81GluThr: 2.81 ± 0.047
3.578GluVal: 3.578 ± 0.062
0.577GluTrp: 0.577 ± 0.021
2.063GluTyr: 2.063 ± 0.041
0.001GluXaa: 0.001 ± 0.001
Phe
3.591PheAla: 3.591 ± 0.058
0.438PheCys: 0.438 ± 0.017
2.861PheAsp: 2.861 ± 0.049
2.547PheGlu: 2.547 ± 0.04
2.318PhePhe: 2.318 ± 0.06
3.376PheGly: 3.376 ± 0.055
0.785PheHis: 0.785 ± 0.026
3.462PheIle: 3.462 ± 0.062
3.313PheLys: 3.313 ± 0.046
4.025PheLeu: 4.025 ± 0.068
1.128PheMet: 1.128 ± 0.03
3.021PheAsn: 3.021 ± 0.06
1.637PhePro: 1.637 ± 0.035
1.177PheGln: 1.177 ± 0.03
1.808PheArg: 1.808 ± 0.042
3.368PheSer: 3.368 ± 0.063
3.162PheThr: 3.162 ± 0.056
2.854PheVal: 2.854 ± 0.054
0.557PheTrp: 0.557 ± 0.023
2.074PheTyr: 2.074 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.878GlyAla: 4.878 ± 0.079
0.584GlyCys: 0.584 ± 0.024
3.514GlyAsp: 3.514 ± 0.063
3.325GlyGlu: 3.325 ± 0.055
3.626GlyPhe: 3.626 ± 0.056
4.874GlyGly: 4.874 ± 0.089
1.426GlyHis: 1.426 ± 0.036
5.353GlyIle: 5.353 ± 0.063
5.214GlyLys: 5.214 ± 0.073
6.252GlyLeu: 6.252 ± 0.072
1.723GlyMet: 1.723 ± 0.042
3.792GlyAsn: 3.792 ± 0.07
1.672GlyPro: 1.672 ± 0.039
2.373GlyGln: 2.373 ± 0.044
2.771GlyArg: 2.771 ± 0.044
4.278GlySer: 4.278 ± 0.067
4.197GlyThr: 4.197 ± 0.069
4.552GlyVal: 4.552 ± 0.066
0.973GlyTrp: 0.973 ± 0.026
3.197GlyTyr: 3.197 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.292HisAla: 1.292 ± 0.032
0.208HisCys: 0.208 ± 0.013
1.02HisAsp: 1.02 ± 0.027
1.021HisGlu: 1.021 ± 0.033
1.109HisPhe: 1.109 ± 0.03
1.209HisGly: 1.209 ± 0.037
0.63HisHis: 0.63 ± 0.027
1.478HisIle: 1.478 ± 0.032
1.113HisLys: 1.113 ± 0.027
1.989HisLeu: 1.989 ± 0.041
0.428HisMet: 0.428 ± 0.019
1.011HisAsn: 1.011 ± 0.027
1.052HisPro: 1.052 ± 0.028
0.815HisGln: 0.815 ± 0.027
0.823HisArg: 0.823 ± 0.024
1.037HisSer: 1.037 ± 0.029
1.075HisThr: 1.075 ± 0.029
1.099HisVal: 1.099 ± 0.036
0.271HisTrp: 0.271 ± 0.016
0.91HisTyr: 0.91 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.181IleAla: 6.181 ± 0.068
0.697IleCys: 0.697 ± 0.024
4.197IleAsp: 4.197 ± 0.05
3.772IleGlu: 3.772 ± 0.063
3.05IlePhe: 3.05 ± 0.061
4.784IleGly: 4.784 ± 0.071
1.254IleHis: 1.254 ± 0.032
5.103IleIle: 5.103 ± 0.078
4.999IleLys: 4.999 ± 0.068
5.922IleLeu: 5.922 ± 0.086
1.412IleMet: 1.412 ± 0.039
4.344IleAsn: 4.344 ± 0.067
3.0IlePro: 3.0 ± 0.048
2.152IleGln: 2.152 ± 0.042
2.929IleArg: 2.929 ± 0.052
4.832IleSer: 4.832 ± 0.065
4.704IleThr: 4.704 ± 0.062
4.215IleVal: 4.215 ± 0.059
0.705IleTrp: 0.705 ± 0.025
2.66IleTyr: 2.66 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
5.782LysAla: 5.782 ± 0.073
0.321LysCys: 0.321 ± 0.016
3.83LysAsp: 3.83 ± 0.06
4.154LysGlu: 4.154 ± 0.065
2.681LysPhe: 2.681 ± 0.049
4.461LysGly: 4.461 ± 0.069
1.349LysHis: 1.349 ± 0.038
4.541LysIle: 4.541 ± 0.064
5.137LysLys: 5.137 ± 0.077
6.349LysLeu: 6.349 ± 0.076
1.814LysMet: 1.814 ± 0.043
3.973LysAsn: 3.973 ± 0.053
3.047LysPro: 3.047 ± 0.056
3.107LysGln: 3.107 ± 0.055
2.896LysArg: 2.896 ± 0.048
3.562LysSer: 3.562 ± 0.054
4.026LysThr: 4.026 ± 0.06
4.421LysVal: 4.421 ± 0.07
0.802LysTrp: 0.802 ± 0.025
2.76LysTyr: 2.76 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.135LeuAla: 7.135 ± 0.075
0.822LeuCys: 0.822 ± 0.03
4.661LeuAsp: 4.661 ± 0.059
4.443LeuGlu: 4.443 ± 0.061
4.609LeuPhe: 4.609 ± 0.074
5.744LeuGly: 5.744 ± 0.07
1.858LeuHis: 1.858 ± 0.037
6.341LeuIle: 6.341 ± 0.088
7.169LeuLys: 7.169 ± 0.092
9.532LeuLeu: 9.532 ± 0.128
2.338LeuMet: 2.338 ± 0.05
5.63LeuAsn: 5.63 ± 0.074
4.149LeuPro: 4.149 ± 0.063
3.814LeuGln: 3.814 ± 0.061
3.764LeuArg: 3.764 ± 0.055
6.61LeuSer: 6.61 ± 0.071
5.521LeuThr: 5.521 ± 0.071
5.59LeuVal: 5.59 ± 0.077
0.942LeuTrp: 0.942 ± 0.033
3.341LeuTyr: 3.341 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.115MetAla: 2.115 ± 0.05
0.147MetCys: 0.147 ± 0.012
1.226MetAsp: 1.226 ± 0.035
1.289MetGlu: 1.289 ± 0.033
0.881MetPhe: 0.881 ± 0.026
1.588MetGly: 1.588 ± 0.04
0.476MetHis: 0.476 ± 0.019
1.508MetIle: 1.508 ± 0.038
1.885MetLys: 1.885 ± 0.04
2.409MetLeu: 2.409 ± 0.043
0.665MetMet: 0.665 ± 0.027
1.233MetAsn: 1.233 ± 0.029
1.158MetPro: 1.158 ± 0.036
1.063MetGln: 1.063 ± 0.029
1.096MetArg: 1.096 ± 0.028
1.361MetSer: 1.361 ± 0.033
1.039MetThr: 1.039 ± 0.028
1.676MetVal: 1.676 ± 0.043
0.158MetTrp: 0.158 ± 0.011
0.692MetTyr: 0.692 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
4.304AsnAla: 4.304 ± 0.072
0.421AsnCys: 0.421 ± 0.018
2.937AsnAsp: 2.937 ± 0.049
2.829AsnGlu: 2.829 ± 0.046
2.625AsnPhe: 2.625 ± 0.053
4.236AsnGly: 4.236 ± 0.07
1.035AsnHis: 1.035 ± 0.026
4.127AsnIle: 4.127 ± 0.056
3.672AsnLys: 3.672 ± 0.058
4.854AsnLeu: 4.854 ± 0.076
1.212AsnMet: 1.212 ± 0.029
3.592AsnAsn: 3.592 ± 0.062
2.657AsnPro: 2.657 ± 0.046
2.119AsnGln: 2.119 ± 0.041
2.442AsnArg: 2.442 ± 0.049
3.16AsnSer: 3.16 ± 0.063
3.32AsnThr: 3.32 ± 0.064
3.286AsnVal: 3.286 ± 0.062
0.738AsnTrp: 0.738 ± 0.026
2.801AsnTyr: 2.801 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
3.656ProAla: 3.656 ± 0.06
0.234ProCys: 0.234 ± 0.014
2.723ProAsp: 2.723 ± 0.049
2.809ProGlu: 2.809 ± 0.049
1.859ProPhe: 1.859 ± 0.038
2.96ProGly: 2.96 ± 0.05
0.754ProHis: 0.754 ± 0.023
2.211ProIle: 2.211 ± 0.043
2.189ProLys: 2.189 ± 0.042
3.366ProLeu: 3.366 ± 0.05
0.849ProMet: 0.849 ± 0.026
1.985ProAsn: 1.985 ± 0.041
1.184ProPro: 1.184 ± 0.036
1.585ProGln: 1.585 ± 0.036
1.166ProArg: 1.166 ± 0.03
2.154ProSer: 2.154 ± 0.049
1.955ProThr: 1.955 ± 0.043
3.505ProVal: 3.505 ± 0.061
0.438ProTrp: 0.438 ± 0.02
1.654ProTyr: 1.654 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.892GlnAla: 2.892 ± 0.054
0.201GlnCys: 0.201 ± 0.012
1.675GlnAsp: 1.675 ± 0.039
1.911GlnGlu: 1.911 ± 0.042
1.643GlnPhe: 1.643 ± 0.034
2.06GlnGly: 2.06 ± 0.039
0.907GlnHis: 0.907 ± 0.026
2.453GlnIle: 2.453 ± 0.041
2.738GlnLys: 2.738 ± 0.054
3.992GlnLeu: 3.992 ± 0.054
0.974GlnMet: 0.974 ± 0.032
2.076GlnAsn: 2.076 ± 0.046
1.733GlnPro: 1.733 ± 0.036
2.369GlnGln: 2.369 ± 0.055
1.644GlnArg: 1.644 ± 0.036
2.24GlnSer: 2.24 ± 0.039
2.27GlnThr: 2.27 ± 0.038
2.441GlnVal: 2.441 ± 0.042
0.43GlnTrp: 0.43 ± 0.018
1.637GlnTyr: 1.637 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
2.653ArgAla: 2.653 ± 0.047
0.254ArgCys: 0.254 ± 0.017
2.181ArgAsp: 2.181 ± 0.046
2.305ArgGlu: 2.305 ± 0.047
2.138ArgPhe: 2.138 ± 0.041
2.319ArgGly: 2.319 ± 0.046
0.812ArgHis: 0.812 ± 0.027
3.118ArgIle: 3.118 ± 0.051
2.895ArgLys: 2.895 ± 0.048
4.126ArgLeu: 4.126 ± 0.063
1.091ArgMet: 1.091 ± 0.029
2.326ArgAsn: 2.326 ± 0.048
1.489ArgPro: 1.489 ± 0.036
1.729ArgGln: 1.729 ± 0.033
1.794ArgArg: 1.794 ± 0.041
2.396ArgSer: 2.396 ± 0.049
2.075ArgThr: 2.075 ± 0.045
2.607ArgVal: 2.607 ± 0.049
0.506ArgTrp: 0.506 ± 0.022
1.998ArgTyr: 1.998 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
4.92SerAla: 4.92 ± 0.074
0.481SerCys: 0.481 ± 0.019
2.844SerAsp: 2.844 ± 0.054
2.799SerGlu: 2.799 ± 0.055
3.271SerPhe: 3.271 ± 0.053
4.697SerGly: 4.697 ± 0.07
1.07SerHis: 1.07 ± 0.026
4.31SerIle: 4.31 ± 0.063
3.635SerLys: 3.635 ± 0.057
5.786SerLeu: 5.786 ± 0.075
1.271SerMet: 1.271 ± 0.028
3.054SerAsn: 3.054 ± 0.056
2.371SerPro: 2.371 ± 0.038
1.919SerGln: 1.919 ± 0.042
2.517SerArg: 2.517 ± 0.049
3.617SerSer: 3.617 ± 0.073
3.473SerThr: 3.473 ± 0.069
4.236SerVal: 4.236 ± 0.065
0.674SerTrp: 0.674 ± 0.022
2.628SerTyr: 2.628 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
5.012ThrAla: 5.012 ± 0.079
0.386ThrCys: 0.386 ± 0.017
3.639ThrAsp: 3.639 ± 0.053
3.001ThrGlu: 3.001 ± 0.052
2.67ThrPhe: 2.67 ± 0.047
4.96ThrGly: 4.96 ± 0.072
1.059ThrHis: 1.059 ± 0.03
4.3ThrIle: 4.3 ± 0.058
2.979ThrLys: 2.979 ± 0.053
5.397ThrLeu: 5.397 ± 0.076
1.047ThrMet: 1.047 ± 0.03
2.871ThrAsn: 2.871 ± 0.057
2.763ThrPro: 2.763 ± 0.055
1.905ThrGln: 1.905 ± 0.044
2.153ThrArg: 2.153 ± 0.044
3.376ThrSer: 3.376 ± 0.055
3.559ThrThr: 3.559 ± 0.071
4.161ThrVal: 4.161 ± 0.067
0.644ThrTrp: 0.644 ± 0.025
2.44ThrTyr: 2.44 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
5.045ValAla: 5.045 ± 0.066
0.626ValCys: 0.626 ± 0.023
3.472ValAsp: 3.472 ± 0.05
3.294ValGlu: 3.294 ± 0.056
3.096ValPhe: 3.096 ± 0.055
3.783ValGly: 3.783 ± 0.066
1.062ValHis: 1.062 ± 0.031
4.896ValIle: 4.896 ± 0.068
4.799ValLys: 4.799 ± 0.067
6.17ValLeu: 6.17 ± 0.08
1.6ValMet: 1.6 ± 0.04
3.931ValAsn: 3.931 ± 0.062
2.557ValPro: 2.557 ± 0.046
2.095ValGln: 2.095 ± 0.04
2.363ValArg: 2.363 ± 0.047
4.211ValSer: 4.211 ± 0.058
4.279ValThr: 4.279 ± 0.073
4.435ValVal: 4.435 ± 0.066
0.697ValTrp: 0.697 ± 0.025
2.618ValTyr: 2.618 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.811TrpAla: 0.811 ± 0.032
0.13TrpCys: 0.13 ± 0.011
0.65TrpAsp: 0.65 ± 0.025
0.588TrpGlu: 0.588 ± 0.02
0.622TrpPhe: 0.622 ± 0.025
0.822TrpGly: 0.822 ± 0.027
0.272TrpHis: 0.272 ± 0.014
0.688TrpIle: 0.688 ± 0.024
0.744TrpLys: 0.744 ± 0.025
1.222TrpLeu: 1.222 ± 0.033
0.347TrpMet: 0.347 ± 0.019
0.59TrpAsn: 0.59 ± 0.02
0.382TrpPro: 0.382 ± 0.019
0.584TrpGln: 0.584 ± 0.026
0.532TrpArg: 0.532 ± 0.021
0.577TrpSer: 0.577 ± 0.022
0.571TrpThr: 0.571 ± 0.021
0.721TrpVal: 0.721 ± 0.027
0.181TrpTrp: 0.181 ± 0.012
0.447TrpTyr: 0.447 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.959TyrAla: 2.959 ± 0.055
0.339TyrCys: 0.339 ± 0.018
2.291TyrAsp: 2.291 ± 0.049
1.933TyrGlu: 1.933 ± 0.042
2.188TyrPhe: 2.188 ± 0.044
2.9TyrGly: 2.9 ± 0.054
1.005TyrHis: 1.005 ± 0.031
2.628TyrIle: 2.628 ± 0.054
2.676TyrLys: 2.676 ± 0.05
4.056TyrLeu: 4.056 ± 0.066
0.815TyrMet: 0.815 ± 0.023
2.596TyrAsn: 2.596 ± 0.044
1.67TyrPro: 1.67 ± 0.036
1.782TyrGln: 1.782 ± 0.044
2.046TyrArg: 2.046 ± 0.036
2.559TyrSer: 2.559 ± 0.049
2.631TyrThr: 2.631 ± 0.052
2.302TyrVal: 2.302 ± 0.046
0.525TyrTrp: 0.525 ± 0.023
2.025TyrTyr: 2.025 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.001
0.001XaaThr: 0.001 ± 0.001
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3800 proteins (1281753 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski