Tortoise microvirus 81
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6Y4|A0A4P8W6Y4_9VIRU Major capsid protein OS=Tortoise microvirus 81 OX=2583189 PE=4 SV=1
MM1 pKa = 7.93 AYY3 pKa = 10.41 YY4 pKa = 10.16 DD5 pKa = 4.56 LKK7 pKa = 11.21 SISDD11 pKa = 3.63 WGVVASGEE19 pKa = 4.18 FHH21 pKa = 7.77 DD22 pKa = 5.92 LPFNNGRR29 pKa = 11.84 TTFNRR34 pKa = 11.84 TIITSHH40 pKa = 5.41 EE41 pKa = 4.17 VFVQVQGLNVDD52 pKa = 3.94 PEE54 pKa = 4.47 SGEE57 pKa = 4.0 VFAPLDD63 pKa = 3.51 FFMTVPPGMHH73 pKa = 7.4 DD74 pKa = 3.26 LTFNLDD80 pKa = 3.86 QPAKK84 pKa = 9.01 WGWYY88 pKa = 7.45 LQPGAPADD96 pKa = 3.49 GANYY100 pKa = 9.92 VRR102 pKa = 11.84 FRR104 pKa = 11.84 NNIRR108 pKa = 11.84 DD109 pKa = 3.78 QVIVPPAEE117 pKa = 4.9 DD118 pKa = 3.48 SLTTIEE124 pKa = 4.09 PAAPRR129 pKa = 11.84 NLEE132 pKa = 3.79 ADD134 pKa = 4.0 RR135 pKa = 11.84 QAMLQRR141 pKa = 11.84 YY142 pKa = 8.11 NLAMLQRR149 pKa = 11.84 TLNEE153 pKa = 3.77 EE154 pKa = 3.89 LDD156 pKa = 3.4 ALRR159 pKa = 11.84 AQRR162 pKa = 11.84 AAAAEE167 pKa = 4.14 APPVVEE173 pKa = 5.01 DD174 pKa = 4.03 TPEE177 pKa = 4.22 GQSS180 pKa = 3.1
Molecular weight: 20.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.538
IPC2_protein 4.469
IPC_protein 4.393
Toseland 4.228
ProMoST 4.52
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.24
Rodwell 4.228
Grimsley 4.139
Solomon 4.355
Lehninger 4.304
Nozaki 4.469
DTASelect 4.647
Thurlkill 4.24
EMBOSS 4.266
Sillero 4.507
Patrickios 3.923
IPC_peptide 4.355
IPC2_peptide 4.495
IPC2.peptide.svr19 4.441
Protein with the highest isoelectric point:
>tr|A0A4P8W7S8|A0A4P8W7S8_9VIRU Uncharacterized protein OS=Tortoise microvirus 81 OX=2583189 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 TYY6 pKa = 11.03 PMARR10 pKa = 11.84 KK11 pKa = 9.45 GKK13 pKa = 10.03 RR14 pKa = 11.84 NRR16 pKa = 11.84 TQKK19 pKa = 7.28 TTVRR23 pKa = 11.84 AGGQTLTATVVRR35 pKa = 11.84 ATPEE39 pKa = 3.55 PMKK42 pKa = 10.2 PRR44 pKa = 11.84 RR45 pKa = 11.84 SLTLARR51 pKa = 11.84 DD52 pKa = 3.58 RR53 pKa = 11.84 DD54 pKa = 4.05 AGAHH58 pKa = 4.69 VPRR61 pKa = 11.84 TISPAPFAVPAVATRR76 pKa = 11.84 TTRR79 pKa = 11.84 DD80 pKa = 2.74 IQAQQKK86 pKa = 9.68 AVKK89 pKa = 9.42 QRR91 pKa = 11.84 RR92 pKa = 11.84 AAQNTQQDD100 pKa = 3.92 NPSSKK105 pKa = 9.31 RR106 pKa = 11.84 TRR108 pKa = 11.84 GVCKK112 pKa = 9.55 PQPDD116 pKa = 3.8 PAKK119 pKa = 10.25 APRR122 pKa = 11.84 GGGGSRR128 pKa = 11.84 PFVPWCLL135 pKa = 3.44
Molecular weight: 14.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.19
IPC2_protein 10.394
IPC_protein 11.886
Toseland 12.062
ProMoST 12.544
Dawson 12.062
Bjellqvist 12.047
Wikipedia 12.53
Rodwell 11.798
Grimsley 12.106
Solomon 12.544
Lehninger 12.457
Nozaki 12.062
DTASelect 12.047
Thurlkill 12.062
EMBOSS 12.559
Sillero 12.062
Patrickios 11.52
IPC_peptide 12.544
IPC2_peptide 11.535
IPC2.peptide.svr19 9.286
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1833
74
539
229.1
25.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.274 ± 0.894
0.927 ± 0.428
5.292 ± 0.624
6.219 ± 0.563
4.255 ± 0.443
8.238 ± 1.421
2.019 ± 0.277
4.474 ± 0.428
4.801 ± 0.56
7.256 ± 0.9
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.346 ± 0.233
4.146 ± 0.595
6.11 ± 0.64
3.71 ± 0.463
7.911 ± 0.855
5.237 ± 0.858
6.274 ± 0.618
6.765 ± 0.691
1.582 ± 0.324
3.164 ± 0.715
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here