Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina; Methanosarcina acetivorans

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4468 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8TII5|Q8TII5_METAC Corrinoid protein OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=MA_4164 PE=3 SV=1
MM1 pKa = 7.45EE2 pKa = 5.58RR3 pKa = 11.84FNMKK7 pKa = 8.87LTRR10 pKa = 11.84KK11 pKa = 9.82KK12 pKa = 9.84FTGNLLRR19 pKa = 11.84SLAVLLLFSLVVCTAAGEE37 pKa = 4.11EE38 pKa = 5.24LYY40 pKa = 10.81FEE42 pKa = 4.37PQEE45 pKa = 4.55AVFSDD50 pKa = 3.71TGSVASLSLYY60 pKa = 10.47LDD62 pKa = 3.56QAPSGLAGYY71 pKa = 9.64KK72 pKa = 10.39LNLSISDD79 pKa = 3.77PSVAQITGVEE89 pKa = 4.52FPSWSGINNSSSLPADD105 pKa = 3.96LVQIKK110 pKa = 10.35AVDD113 pKa = 3.84LEE115 pKa = 4.3EE116 pKa = 5.16KK117 pKa = 10.57INAGDD122 pKa = 3.95LDD124 pKa = 4.16VFLGTVTVEE133 pKa = 4.11SLASGEE139 pKa = 4.36ANLTVSVDD147 pKa = 3.51RR148 pKa = 11.84LDD150 pKa = 5.06DD151 pKa = 4.42DD152 pKa = 4.25SEE154 pKa = 4.23NTISVTVRR162 pKa = 11.84EE163 pKa = 4.53AKK165 pKa = 9.11LTSGTTSEE173 pKa = 4.38NPVVSILPAEE183 pKa = 4.37STLSSGASQVFEE195 pKa = 3.66IRR197 pKa = 11.84ADD199 pKa = 3.37KK200 pKa = 9.45FTEE203 pKa = 5.06GISGYY208 pKa = 10.7DD209 pKa = 3.41FNLTLEE215 pKa = 4.38NPAVGKK221 pKa = 7.7FTSVEE226 pKa = 4.01YY227 pKa = 9.91PSWVGLSEE235 pKa = 4.06NSSMPNTSIKK245 pKa = 10.49VKK247 pKa = 10.67AVDD250 pKa = 3.49TNDD253 pKa = 3.03VVKK256 pKa = 11.07AGDD259 pKa = 3.96EE260 pKa = 4.18NVLLANVTFVAEE272 pKa = 4.4TPGEE276 pKa = 4.23SEE278 pKa = 4.29ISLTFNRR285 pKa = 11.84LDD287 pKa = 4.57DD288 pKa = 5.0DD289 pKa = 4.09SGSNINAATEE299 pKa = 3.85ASSIKK304 pKa = 10.18VQGSSTLPVAKK315 pKa = 9.39FTATPTSGDD324 pKa = 3.37SPLTVQFTDD333 pKa = 3.65EE334 pKa = 4.5STGSPTAWAWDD345 pKa = 3.58FDD347 pKa = 3.88NDD349 pKa = 3.97GNVDD353 pKa = 3.53STEE356 pKa = 3.96QNPSYY361 pKa = 9.86TYY363 pKa = 10.89SDD365 pKa = 3.0AGIYY369 pKa = 7.29TVKK372 pKa = 10.01LTVSNEE378 pKa = 3.76NGSDD382 pKa = 3.42VLEE385 pKa = 4.24ILEE388 pKa = 4.63MITVKK393 pKa = 9.6EE394 pKa = 4.33TVVSEE399 pKa = 4.07DD400 pKa = 2.44AWYY403 pKa = 10.4QFHH406 pKa = 7.95KK407 pKa = 10.6DD408 pKa = 3.68AQHH411 pKa = 6.21SGYY414 pKa = 10.25SSSDD418 pKa = 3.36APDD421 pKa = 3.54SANLAWIAEE430 pKa = 4.36PLNNTYY436 pKa = 11.18SLVPSSSVVIAEE448 pKa = 3.76GMVFGLCNGPVDD460 pKa = 4.47EE461 pKa = 5.13YY462 pKa = 11.62GNPLTSEE469 pKa = 4.28GQLVAFDD476 pKa = 4.38EE477 pKa = 4.63EE478 pKa = 4.49TGEE481 pKa = 4.36EE482 pKa = 3.87IWNVTVMAPEE492 pKa = 4.18WGSWSCPAYY501 pKa = 10.52DD502 pKa = 4.28DD503 pKa = 4.46GKK505 pKa = 11.12VFASAGKK512 pKa = 8.23NTYY515 pKa = 9.56CVNASTGDD523 pKa = 4.0IIWTFQNPSEE533 pKa = 4.11LASCNGGPSIGDD545 pKa = 3.32GKK547 pKa = 11.05VFASDD552 pKa = 3.14WDD554 pKa = 3.72GGNYY558 pKa = 9.61YY559 pKa = 10.69CLDD562 pKa = 3.88EE563 pKa = 4.38NTGEE567 pKa = 4.31LLWTFKK573 pKa = 10.52IDD575 pKa = 3.34GLYY578 pKa = 10.5AQSTPAYY585 pKa = 9.06KK586 pKa = 10.01DD587 pKa = 3.13DD588 pKa = 3.61RR589 pKa = 11.84VYY591 pKa = 11.31LSGWTTVNAVYY602 pKa = 10.19CVNATTGEE610 pKa = 4.42LIWEE614 pKa = 4.14NEE616 pKa = 4.06EE617 pKa = 5.08LSSNPCGSITVTEE630 pKa = 4.14EE631 pKa = 3.55GLYY634 pKa = 10.7LSIYY638 pKa = 10.78SFGTEE643 pKa = 3.7DD644 pKa = 4.02GFYY647 pKa = 10.95KK648 pKa = 10.66LDD650 pKa = 3.54LTDD653 pKa = 3.63GHH655 pKa = 6.55EE656 pKa = 3.74IWGRR660 pKa = 11.84PDD662 pKa = 3.15IPPTDD667 pKa = 3.61STPAVVNGKK676 pKa = 9.95VYY678 pKa = 10.73LSAGTAGYY686 pKa = 10.65SDD688 pKa = 5.15LNTYY692 pKa = 9.69CLNASDD698 pKa = 5.71GKK700 pKa = 8.81TIWVTDD706 pKa = 3.42SSEE709 pKa = 4.66NIGDD713 pKa = 4.16WVCSPAVADD722 pKa = 4.09GKK724 pKa = 11.11VFTGGAAEE732 pKa = 4.22GLFTGSSTLYY742 pKa = 11.0AFDD745 pKa = 4.96AEE747 pKa = 4.4TGAVIWSYY755 pKa = 10.61KK756 pKa = 9.57GCGGSPAVADD766 pKa = 4.07DD767 pKa = 4.17MVFSTGSGKK776 pKa = 10.73LYY778 pKa = 10.35AFKK781 pKa = 10.26EE782 pKa = 4.34AEE784 pKa = 4.19VLLPEE789 pKa = 4.96AKK791 pKa = 10.07FSSNVSSGEE800 pKa = 3.81APLTVGFTDD809 pKa = 3.45EE810 pKa = 4.4STGEE814 pKa = 4.87GITAWEE820 pKa = 3.95WDD822 pKa = 3.46FDD824 pKa = 3.86NDD826 pKa = 3.98GNVDD830 pKa = 3.55STEE833 pKa = 3.89QNPVHH838 pKa = 6.46TYY840 pKa = 11.42DD841 pKa = 3.18NAGSYY846 pKa = 7.95TVNLTVTSAEE856 pKa = 4.25GSDD859 pKa = 4.01SEE861 pKa = 5.52VKK863 pKa = 9.5MDD865 pKa = 4.24YY866 pKa = 10.95INVSEE871 pKa = 4.51SSTPGEE877 pKa = 4.32PEE879 pKa = 3.49PVAAFIANVTSGTAPLTVNFTDD901 pKa = 3.66QSTGSPTSWEE911 pKa = 3.65WDD913 pKa = 3.09FDD915 pKa = 3.72NDD917 pKa = 3.98GNVDD921 pKa = 3.55STEE924 pKa = 3.89QNPVHH929 pKa = 6.94TYY931 pKa = 8.49TAAGNYY937 pKa = 7.27TVSLTVTNAEE947 pKa = 4.26GSDD950 pKa = 3.81SEE952 pKa = 4.85VKK954 pKa = 10.45ADD956 pKa = 3.84YY957 pKa = 9.24ITVSEE962 pKa = 4.38ASTPVEE968 pKa = 4.27PEE970 pKa = 3.4PVAAFTADD978 pKa = 3.7VTSGTAPLTVNFTDD992 pKa = 3.66QSTGSPTAWAWDD1004 pKa = 3.58FDD1006 pKa = 3.88NDD1008 pKa = 3.97GNVDD1012 pKa = 3.53STEE1015 pKa = 3.96QNPSYY1020 pKa = 10.15TYY1022 pKa = 11.23NDD1024 pKa = 3.46AGSYY1028 pKa = 8.11TVNLTVTNAEE1038 pKa = 4.12GSNSEE1043 pKa = 4.22VKK1045 pKa = 9.78TEE1047 pKa = 4.16YY1048 pKa = 9.41ITVEE1052 pKa = 4.16EE1053 pKa = 4.59ASSGSQNGSSSVSLNVTIVPVISLEE1078 pKa = 4.14VSPSALDD1085 pKa = 3.64FGEE1088 pKa = 4.85LYY1090 pKa = 10.1PGKK1093 pKa = 9.05TSEE1096 pKa = 4.51LQYY1099 pKa = 10.48LTLKK1103 pKa = 10.63NRR1105 pKa = 11.84GSCDD1109 pKa = 3.14INVTAVVCDD1118 pKa = 4.63CSAEE1122 pKa = 4.34DD1123 pKa = 3.66EE1124 pKa = 4.78LFSQGLLLDD1133 pKa = 3.76SQLWNNYY1140 pKa = 6.04WKK1142 pKa = 10.9VVGKK1146 pKa = 10.21NSQEE1150 pKa = 4.02NTSVALQVPADD1161 pKa = 3.82YY1162 pKa = 10.99AGSGNKK1168 pKa = 9.51KK1169 pKa = 10.42GSITFWAEE1177 pKa = 3.08AAEE1180 pKa = 4.12

Molecular weight: 125.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8TIW5|Q8TIW5_METAC Uncharacterized protein OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=MA_4025 PE=4 SV=1
MM1 pKa = 6.8SHH3 pKa = 6.05NMKK6 pKa = 10.11GQKK9 pKa = 9.42KK10 pKa = 9.38RR11 pKa = 11.84LAKK14 pKa = 10.01AHH16 pKa = 5.8KK17 pKa = 9.35QNTRR21 pKa = 11.84VPVWVIVKK29 pKa = 7.36TNRR32 pKa = 11.84KK33 pKa = 8.65VVSHH37 pKa = 6.09PRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.43WRR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84SLDD49 pKa = 3.3VKK51 pKa = 11.01

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4468

0

4468

1392316

41

4226

311.6

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.926 ± 0.044

1.251 ± 0.018

5.35 ± 0.026

7.966 ± 0.048

4.434 ± 0.031

7.263 ± 0.041

1.659 ± 0.015

7.35 ± 0.038

6.498 ± 0.052

9.406 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.021

4.474 ± 0.038

4.006 ± 0.023

2.533 ± 0.019

4.465 ± 0.039

6.907 ± 0.042

5.442 ± 0.049

6.843 ± 0.034

1.052 ± 0.013

3.718 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski