Amino acid dipepetide frequency for Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.714AlaAla: 5.714 ± 0.098	0.924AlaCys: 0.924 ± 0.028	3.676AlaAsp: 3.676 ± 0.056	5.424AlaGlu: 5.424 ± 0.08	3.11AlaPhe: 3.11 ± 0.064	6.032AlaGly: 6.032 ± 0.089	1.044AlaHis: 1.044 ± 0.03	4.714AlaIle: 4.714 ± 0.065	3.779AlaLys: 3.779 ± 0.06	7.16AlaLeu: 7.16 ± 0.118	1.662AlaMet: 1.662 ± 0.036	2.461AlaAsn: 2.461 ± 0.052	2.247AlaPro: 2.247 ± 0.047	1.587AlaGln: 1.587 ± 0.036	3.235AlaArg: 3.235 ± 0.053	4.559AlaSer: 4.559 ± 0.07	3.221AlaThr: 3.221 ± 0.056	5.575AlaVal: 5.575 ± 0.073	0.735AlaTrp: 0.735 ± 0.031	2.4AlaTyr: 2.4 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.747CysAla: 0.747 ± 0.025	0.231CysCys: 0.231 ± 0.017	0.619CysAsp: 0.619 ± 0.024	0.738CysGlu: 0.738 ± 0.027	0.563CysPhe: 0.563 ± 0.02	1.225CysGly: 1.225 ± 0.033	0.243CysHis: 0.243 ± 0.015	0.836CysIle: 0.836 ± 0.027	0.728CysLys: 0.728 ± 0.026	1.006CysLeu: 1.006 ± 0.027	0.349CysMet: 0.349 ± 0.016	0.49CysAsn: 0.49 ± 0.018	0.764CysPro: 0.764 ± 0.029	0.297CysGln: 0.297 ± 0.017	0.718CysArg: 0.718 ± 0.027	0.897CysSer: 0.897 ± 0.032	0.747CysThr: 0.747 ± 0.03	0.727CysVal: 0.727 ± 0.027	0.113CysTrp: 0.113 ± 0.008	0.467CysTyr: 0.467 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	3.463AspAla: 3.463 ± 0.062	0.655AspCys: 0.655 ± 0.025	2.227AspAsp: 2.227 ± 0.058	3.97AspGlu: 3.97 ± 0.06	2.876AspPhe: 2.876 ± 0.045	3.93AspGly: 3.93 ± 0.099	0.794AspHis: 0.794 ± 0.021	4.386AspIle: 4.386 ± 0.06	2.836AspLys: 2.836 ± 0.05	5.209AspLeu: 5.209 ± 0.078	1.365AspMet: 1.365 ± 0.032	2.138AspAsn: 2.138 ± 0.048	2.579AspPro: 2.579 ± 0.046	1.194AspGln: 1.194 ± 0.031	2.603AspArg: 2.603 ± 0.047	3.998AspSer: 3.998 ± 0.073	2.794AspThr: 2.794 ± 0.056	3.653AspVal: 3.653 ± 0.058	0.613AspTrp: 0.613 ± 0.023	2.215AspTyr: 2.215 ± 0.045	0.001AspXaa: 0.001 ± 0.001
Glu	5.674GluAla: 5.674 ± 0.09	0.737GluCys: 0.737 ± 0.026	4.118GluAsp: 4.118 ± 0.049	7.33GluGlu: 7.33 ± 0.103	2.975GluPhe: 2.975 ± 0.056	4.824GluGly: 4.824 ± 0.061	1.148GluHis: 1.148 ± 0.03	6.491GluIle: 6.491 ± 0.09	7.187GluLys: 7.187 ± 0.117	7.488GluLeu: 7.488 ± 0.106	2.116GluMet: 2.116 ± 0.046	4.788GluAsn: 4.788 ± 0.088	2.39GluPro: 2.39 ± 0.045	2.004GluGln: 2.004 ± 0.045	3.255GluArg: 3.255 ± 0.067	4.375GluSer: 4.375 ± 0.069	4.306GluThr: 4.306 ± 0.06	4.9GluVal: 4.9 ± 0.059	0.774GluTrp: 0.774 ± 0.025	2.775GluTyr: 2.775 ± 0.049	0.001GluXaa: 0.001 ± 0.001
Phe	2.807PheAla: 2.807 ± 0.055	0.67PheCys: 0.67 ± 0.024	2.544PheAsp: 2.544 ± 0.052	3.343PheGlu: 3.343 ± 0.068	2.224PhePhe: 2.224 ± 0.057	3.27PheGly: 3.27 ± 0.057	0.751PheHis: 0.751 ± 0.022	2.949PheIle: 2.949 ± 0.056	2.188PheLys: 2.188 ± 0.044	4.51PheLeu: 4.51 ± 0.071	1.031PheMet: 1.031 ± 0.029	1.674PheAsn: 1.674 ± 0.041	1.778PhePro: 1.778 ± 0.039	1.068PheGln: 1.068 ± 0.027	2.166PheArg: 2.166 ± 0.049	3.762PheSer: 3.762 ± 0.063	2.601PheThr: 2.601 ± 0.062	2.908PheVal: 2.908 ± 0.049	0.475PheTrp: 0.475 ± 0.017	1.617PheTyr: 1.617 ± 0.038	0.001PheXaa: 0.001 ± 0.001
Gly	4.718GlyAla: 4.718 ± 0.081	1.136GlyCys: 1.136 ± 0.03	3.775GlyAsp: 3.775 ± 0.071	5.061GlyGlu: 5.061 ± 0.068	3.424GlyPhe: 3.424 ± 0.058	4.842GlyGly: 4.842 ± 0.096	1.175GlyHis: 1.175 ± 0.03	6.244GlyIle: 6.244 ± 0.074	5.595GlyLys: 5.595 ± 0.074	6.127GlyLeu: 6.127 ± 0.069	2.027GlyMet: 2.027 ± 0.043	3.659GlyAsn: 3.659 ± 0.079	2.021GlyPro: 2.021 ± 0.045	1.58GlyGln: 1.58 ± 0.034	2.913GlyArg: 2.913 ± 0.05	5.096GlySer: 5.096 ± 0.092	4.61GlyThr: 4.61 ± 0.093	4.799GlyVal: 4.799 ± 0.065	0.819GlyTrp: 0.819 ± 0.027	3.009GlyTyr: 3.009 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.059HisAla: 1.059 ± 0.026	0.267HisCys: 0.267 ± 0.015	0.87HisAsp: 0.87 ± 0.028	1.136HisGlu: 1.136 ± 0.032	0.802HisPhe: 0.802 ± 0.025	1.171HisGly: 1.171 ± 0.03	0.381HisHis: 0.381 ± 0.022	1.204HisIle: 1.204 ± 0.031	0.843HisLys: 0.843 ± 0.024	1.472HisLeu: 1.472 ± 0.038	0.427HisMet: 0.427 ± 0.018	0.674HisAsn: 0.674 ± 0.023	0.965HisPro: 0.965 ± 0.024	0.428HisGln: 0.428 ± 0.019	0.907HisArg: 0.907 ± 0.027	1.1HisSer: 1.1 ± 0.035	0.946HisThr: 0.946 ± 0.03	1.105HisVal: 1.105 ± 0.031	0.201HisTrp: 0.201 ± 0.013	0.628HisTyr: 0.628 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.122IleAla: 5.122 ± 0.075	0.994IleCys: 0.994 ± 0.029	3.945IleAsp: 3.945 ± 0.061	5.573IleGlu: 5.573 ± 0.075	3.591IlePhe: 3.591 ± 0.062	5.259IleGly: 5.259 ± 0.074	1.27IleHis: 1.27 ± 0.035	5.022IleIle: 5.022 ± 0.077	4.452IleLys: 4.452 ± 0.072	6.827IleLeu: 6.827 ± 0.093	1.624IleMet: 1.624 ± 0.033	3.042IleAsn: 3.042 ± 0.05	3.509IlePro: 3.509 ± 0.054	1.898IleGln: 1.898 ± 0.045	3.445IleArg: 3.445 ± 0.054	5.627IleSer: 5.627 ± 0.064	4.069IleThr: 4.069 ± 0.06	4.758IleVal: 4.758 ± 0.068	0.69IleTrp: 0.69 ± 0.023	2.783IleTyr: 2.783 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	4.651LysAla: 4.651 ± 0.065	0.679LysCys: 0.679 ± 0.026	3.359LysAsp: 3.359 ± 0.055	5.878LysGlu: 5.878 ± 0.09	2.11LysPhe: 2.11 ± 0.043	4.151LysGly: 4.151 ± 0.061	1.049LysHis: 1.049 ± 0.03	5.083LysIle: 5.083 ± 0.076	5.591LysLys: 5.591 ± 0.106	5.617LysLeu: 5.617 ± 0.082	1.732LysMet: 1.732 ± 0.04	3.6LysAsn: 3.6 ± 0.058	2.288LysPro: 2.288 ± 0.043	1.715LysGln: 1.715 ± 0.048	2.846LysArg: 2.846 ± 0.057	3.7LysSer: 3.7 ± 0.053	3.725LysThr: 3.725 ± 0.054	4.25LysVal: 4.25 ± 0.06	0.638LysTrp: 0.638 ± 0.024	2.312LysTyr: 2.312 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	6.733LeuAla: 6.733 ± 0.078	1.026LeuCys: 1.026 ± 0.031	5.105LeuAsp: 5.105 ± 0.069	7.792LeuGlu: 7.792 ± 0.121	4.233LeuPhe: 4.233 ± 0.072	6.504LeuGly: 6.504 ± 0.085	1.543LeuHis: 1.543 ± 0.04	6.695LeuIle: 6.695 ± 0.083	6.847LeuLys: 6.847 ± 0.093	9.17LeuLeu: 9.17 ± 0.122	2.279LeuMet: 2.279 ± 0.048	4.019LeuAsn: 4.019 ± 0.063	3.866LeuPro: 3.866 ± 0.052	2.539LeuGln: 2.539 ± 0.046	4.018LeuArg: 4.018 ± 0.057	6.843LeuSer: 6.843 ± 0.079	4.956LeuThr: 4.956 ± 0.077	5.866LeuVal: 5.866 ± 0.079	0.881LeuTrp: 0.881 ± 0.026	3.142LeuTyr: 3.142 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	1.646MetAla: 1.646 ± 0.044	0.247MetCys: 0.247 ± 0.013	1.408MetAsp: 1.408 ± 0.031	1.908MetGlu: 1.908 ± 0.044	0.919MetPhe: 0.919 ± 0.026	1.89MetGly: 1.89 ± 0.044	0.447MetHis: 0.447 ± 0.02	1.668MetIle: 1.668 ± 0.038	2.02MetLys: 2.02 ± 0.043	2.35MetLeu: 2.35 ± 0.047	0.652MetMet: 0.652 ± 0.023	1.127MetAsn: 1.127 ± 0.024	0.986MetPro: 0.986 ± 0.026	0.799MetGln: 0.799 ± 0.025	1.151MetArg: 1.151 ± 0.028	1.475MetSer: 1.475 ± 0.034	1.301MetThr: 1.301 ± 0.041	1.603MetVal: 1.603 ± 0.031	0.201MetTrp: 0.201 ± 0.014	0.786MetTyr: 0.786 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.856AsnAla: 2.856 ± 0.056	0.616AsnCys: 0.616 ± 0.03	1.815AsnAsp: 1.815 ± 0.043	3.028AsnGlu: 3.028 ± 0.052	2.0AsnPhe: 2.0 ± 0.049	3.117AsnGly: 3.117 ± 0.075	0.63AsnHis: 0.63 ± 0.022	3.449AsnIle: 3.449 ± 0.059	2.33AsnLys: 2.33 ± 0.045	4.454AsnLeu: 4.454 ± 0.07	1.132AsnMet: 1.132 ± 0.033	2.25AsnAsn: 2.25 ± 0.081	2.572AsnPro: 2.572 ± 0.049	1.1AsnGln: 1.1 ± 0.028	2.119AsnArg: 2.119 ± 0.045	3.402AsnSer: 3.402 ± 0.062	2.507AsnThr: 2.507 ± 0.06	3.086AsnVal: 3.086 ± 0.064	0.48AsnTrp: 0.48 ± 0.022	1.798AsnTyr: 1.798 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	3.022ProAla: 3.022 ± 0.053	0.465ProCys: 0.465 ± 0.021	2.84ProAsp: 2.84 ± 0.054	4.917ProGlu: 4.917 ± 0.082	1.698ProPhe: 1.698 ± 0.037	3.117ProGly: 3.117 ± 0.056	0.651ProHis: 0.651 ± 0.024	2.255ProIle: 2.255 ± 0.043	1.897ProLys: 1.897 ± 0.041	3.661ProLeu: 3.661 ± 0.059	0.853ProMet: 0.853 ± 0.024	1.201ProAsn: 1.201 ± 0.031	1.294ProPro: 1.294 ± 0.033	1.029ProGln: 1.029 ± 0.028	1.408ProArg: 1.408 ± 0.041	2.433ProSer: 2.433 ± 0.05	1.857ProThr: 1.857 ± 0.04	3.577ProVal: 3.577 ± 0.07	0.384ProTrp: 0.384 ± 0.017	1.505ProTyr: 1.505 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	1.963GlnAla: 1.963 ± 0.045	0.28GlnCys: 0.28 ± 0.016	1.189GlnAsp: 1.189 ± 0.031	2.127GlnGlu: 2.127 ± 0.047	0.997GlnPhe: 0.997 ± 0.033	1.613GlnGly: 1.613 ± 0.037	0.425GlnHis: 0.425 ± 0.02	2.0GlnIle: 2.0 ± 0.043	2.112GlnLys: 2.112 ± 0.039	2.322GlnLeu: 2.322 ± 0.049	0.667GlnMet: 0.667 ± 0.023	1.39GlnAsn: 1.39 ± 0.04	0.86GlnPro: 0.86 ± 0.026	0.735GlnGln: 0.735 ± 0.026	1.1GlnArg: 1.1 ± 0.03	1.393GlnSer: 1.393 ± 0.036	1.295GlnThr: 1.295 ± 0.034	1.783GlnVal: 1.783 ± 0.041	0.212GlnTrp: 0.212 ± 0.012	0.863GlnTyr: 0.863 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	2.979ArgAla: 2.979 ± 0.055	0.59ArgCys: 0.59 ± 0.026	2.475ArgAsp: 2.475 ± 0.043	3.825ArgGlu: 3.825 ± 0.071	1.987ArgPhe: 1.987 ± 0.037	2.678ArgGly: 2.678 ± 0.048	0.772ArgHis: 0.772 ± 0.025	3.704ArgIle: 3.704 ± 0.06	3.765ArgLys: 3.765 ± 0.07	3.916ArgLeu: 3.916 ± 0.069	1.194ArgMet: 1.194 ± 0.026	2.084ArgAsn: 2.084 ± 0.039	1.426ArgPro: 1.426 ± 0.037	1.281ArgGln: 1.281 ± 0.032	2.069ArgArg: 2.069 ± 0.04	2.458ArgSer: 2.458 ± 0.041	2.176ArgThr: 2.176 ± 0.044	3.009ArgVal: 3.009 ± 0.051	0.468ArgTrp: 0.468 ± 0.019	1.795ArgTyr: 1.795 ± 0.041	0.001ArgXaa: 0.001 ± 0.001
Ser	4.372SerAla: 4.372 ± 0.079	0.764SerCys: 0.764 ± 0.022	3.737SerAsp: 3.737 ± 0.067	5.299SerGlu: 5.299 ± 0.073	3.139SerPhe: 3.139 ± 0.056	6.152SerGly: 6.152 ± 0.081	1.164SerHis: 1.164 ± 0.032	4.885SerIle: 4.885 ± 0.066	3.787SerLys: 3.787 ± 0.063	6.235SerLeu: 6.235 ± 0.071	1.65SerMet: 1.65 ± 0.036	2.76SerAsn: 2.76 ± 0.066	3.011SerPro: 3.011 ± 0.053	1.707SerGln: 1.707 ± 0.033	3.303SerArg: 3.303 ± 0.057	5.503SerSer: 5.503 ± 0.102	3.86SerThr: 3.86 ± 0.097	4.503SerVal: 4.503 ± 0.066	0.891SerTrp: 0.891 ± 0.034	2.351SerTyr: 2.351 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	4.064ThrAla: 4.064 ± 0.075	0.652ThrCys: 0.652 ± 0.024	3.149ThrAsp: 3.149 ± 0.073	3.922ThrGlu: 3.922 ± 0.061	2.216ThrPhe: 2.216 ± 0.042	5.086ThrGly: 5.086 ± 0.094	0.894ThrHis: 0.894 ± 0.023	3.853ThrIle: 3.853 ± 0.066	2.564ThrLys: 2.564 ± 0.042	5.237ThrLeu: 5.237 ± 0.063	1.057ThrMet: 1.057 ± 0.025	2.106ThrAsn: 2.106 ± 0.056	2.519ThrPro: 2.519 ± 0.043	1.258ThrGln: 1.258 ± 0.03	2.093ThrArg: 2.093 ± 0.04	3.619ThrSer: 3.619 ± 0.095	2.745ThrThr: 2.745 ± 0.065	4.625ThrVal: 4.625 ± 0.113	0.547ThrTrp: 0.547 ± 0.022	2.211ThrTyr: 2.211 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	4.56ValAla: 4.56 ± 0.065	0.949ValCys: 0.949 ± 0.028	3.73ValAsp: 3.73 ± 0.06	5.023ValGlu: 5.023 ± 0.072	3.179ValPhe: 3.179 ± 0.05	4.254ValGly: 4.254 ± 0.067	1.304ValHis: 1.304 ± 0.031	4.834ValIle: 4.834 ± 0.061	4.056ValLys: 4.056 ± 0.058	6.857ValLeu: 6.857 ± 0.082	1.625ValMet: 1.625 ± 0.033	2.914ValAsn: 2.914 ± 0.049	3.024ValPro: 3.024 ± 0.049	1.796ValGln: 1.796 ± 0.036	3.034ValArg: 3.034 ± 0.057	5.347ValSer: 5.347 ± 0.082	3.98ValThr: 3.98 ± 0.1	4.67ValVal: 4.67 ± 0.075	0.73ValTrp: 0.73 ± 0.037	2.56ValTyr: 2.56 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.696TrpAla: 0.696 ± 0.028	0.126TrpCys: 0.126 ± 0.009	0.772TrpAsp: 0.772 ± 0.035	0.797TrpGlu: 0.797 ± 0.027	0.403TrpPhe: 0.403 ± 0.017	0.709TrpGly: 0.709 ± 0.027	0.209TrpHis: 0.209 ± 0.013	0.764TrpIle: 0.764 ± 0.024	0.756TrpLys: 0.756 ± 0.027	0.885TrpLeu: 0.885 ± 0.028	0.268TrpMet: 0.268 ± 0.014	0.67TrpAsn: 0.67 ± 0.029	0.259TrpPro: 0.259 ± 0.014	0.335TrpGln: 0.335 ± 0.019	0.393TrpArg: 0.393 ± 0.017	0.629TrpSer: 0.629 ± 0.026	0.608TrpThr: 0.608 ± 0.022	0.657TrpVal: 0.657 ± 0.024	0.152TrpTrp: 0.152 ± 0.011	0.427TrpTyr: 0.427 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.41TyrAla: 2.41 ± 0.056	0.498TyrCys: 0.498 ± 0.022	2.145TyrAsp: 2.145 ± 0.056	2.564TyrGlu: 2.564 ± 0.05	1.892TyrPhe: 1.892 ± 0.044	2.803TyrGly: 2.803 ± 0.056	0.69TyrHis: 0.69 ± 0.023	2.465TyrIle: 2.465 ± 0.048	1.643TyrLys: 1.643 ± 0.042	3.553TyrLeu: 3.553 ± 0.064	0.878TyrMet: 0.878 ± 0.03	1.694TyrAsn: 1.694 ± 0.042	1.702TyrPro: 1.702 ± 0.036	0.976TyrGln: 0.976 ± 0.027	1.874TyrArg: 1.874 ± 0.037	2.858TyrSer: 2.858 ± 0.056	2.114TyrThr: 2.114 ± 0.064	2.369TyrVal: 2.369 ± 0.047	0.514TyrTrp: 0.514 ± 0.021	1.539TyrTyr: 1.539 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4468 proteins (1392316 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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