Cellulophaga lytica (strain ATCC 23178 / DSM 7489 / JCM 8516 / NBRC 14961 / NCIMB 1423 / VKM B-1433 / Cy l20)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Cellulophaga; Cellulophaga lytica

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0RCN7|F0RCN7_CELLC Peptidase S41 OS=Cellulophaga lytica (strain ATCC 23178 / DSM 7489 / JCM 8516 / NBRC 14961 / NCIMB 1423 / VKM B-1433 / Cy l20) OX=867900 GN=Celly_1911 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.05IKK4 pKa = 10.5QLGSFKK10 pKa = 10.46CIKK13 pKa = 9.76KK14 pKa = 9.8QRR16 pKa = 11.84TLSLLVMFFCFSFFAFGQNVSVQRR40 pKa = 11.84TNDD43 pKa = 3.36AEE45 pKa = 4.36EE46 pKa = 4.27TGQVAGSFRR55 pKa = 11.84ISRR58 pKa = 11.84TGNTFTGLRR67 pKa = 11.84VNYY70 pKa = 8.61TITGTATNGTDD81 pKa = 3.38YY82 pKa = 11.61ASIGGSILIPVFNNSINLDD101 pKa = 3.04IDD103 pKa = 3.98GVVDD107 pKa = 3.5DD108 pKa = 4.93TLIEE112 pKa = 4.22GDD114 pKa = 3.59EE115 pKa = 4.36TVIITLSEE123 pKa = 3.72NSEE126 pKa = 4.27YY127 pKa = 11.05NINPLAASARR137 pKa = 11.84IIIVDD142 pKa = 3.95NEE144 pKa = 4.47PVCATAPDD152 pKa = 3.95APEE155 pKa = 4.02LRR157 pKa = 11.84TGEE160 pKa = 4.15EE161 pKa = 4.11TTFCDD166 pKa = 5.51SFTKK170 pKa = 10.62DD171 pKa = 3.11LNDD174 pKa = 3.67YY175 pKa = 9.43VASATPAGTVLTWSSSDD192 pKa = 4.22DD193 pKa = 3.85LLDD196 pKa = 4.3DD197 pKa = 3.53SSYY200 pKa = 11.06YY201 pKa = 10.55DD202 pKa = 3.19SSIIGFSGEE211 pKa = 3.95FYY213 pKa = 11.11GFYY216 pKa = 10.5LDD218 pKa = 4.07EE219 pKa = 5.25ANNCVSPPLKK229 pKa = 9.62ITIVQNSSPTVTGTSDD245 pKa = 3.3SRR247 pKa = 11.84CGEE250 pKa = 3.99GSVTLTGTTTDD261 pKa = 5.67GSLLWYY267 pKa = 8.08DD268 pKa = 3.89APSGGTLVHH277 pKa = 6.66TGLNFDD283 pKa = 4.13TPSLSVTTSYY293 pKa = 11.18YY294 pKa = 10.87VEE296 pKa = 4.17ATANGCTSDD305 pKa = 3.42RR306 pKa = 11.84VEE308 pKa = 4.29VVATINTQPSSGTGTDD324 pKa = 3.19ATACSLPGNGRR335 pKa = 11.84VTVVNLDD342 pKa = 3.81DD343 pKa = 3.98QLTGADD349 pKa = 3.39TGEE352 pKa = 4.17WTLTSQPASSSITIDD367 pKa = 3.28ANSVDD372 pKa = 4.96FANQPLGEE380 pKa = 4.15YY381 pKa = 10.1QFTYY385 pKa = 7.56TTTGFVAPCTAEE397 pKa = 3.91STTITITVLDD407 pKa = 4.56CAPTDD412 pKa = 3.48TDD414 pKa = 4.16LSVTKK419 pKa = 10.37IVDD422 pKa = 3.32RR423 pKa = 11.84DD424 pKa = 3.66EE425 pKa = 4.7VFTGEE430 pKa = 4.53DD431 pKa = 3.57VVFTITVEE439 pKa = 4.04NLTGDD444 pKa = 3.75PVTAIEE450 pKa = 4.25ITDD453 pKa = 3.74VLDD456 pKa = 3.39EE457 pKa = 4.48TTGFEE462 pKa = 4.41YY463 pKa = 10.91VSNIAQLGSFNSATGIWSIAEE484 pKa = 3.99LAAGEE489 pKa = 4.42STSLEE494 pKa = 3.68ITATVIDD501 pKa = 4.8GGTHH505 pKa = 6.23INTAEE510 pKa = 4.06ITSSNPVDD518 pKa = 3.13TATANNQSSATVAIKK533 pKa = 10.45QIDD536 pKa = 3.67MSISKK541 pKa = 10.16SVNKK545 pKa = 8.68VTATVGDD552 pKa = 3.8VVVFTITLTNEE563 pKa = 3.68TDD565 pKa = 3.67GLVTDD570 pKa = 4.81ISVTDD575 pKa = 3.85ILNSTTGFTHH585 pKa = 6.77VSNTTTTGNYY595 pKa = 8.91DD596 pKa = 2.99ATTGVWTLDD605 pKa = 3.53EE606 pKa = 4.19ILGNEE611 pKa = 4.24TQVLEE616 pKa = 4.08ITAMVLEE623 pKa = 5.09EE624 pKa = 4.32GTHH627 pKa = 5.31VNTVEE632 pKa = 4.14ITDD635 pKa = 3.93SFPIDD640 pKa = 3.62EE641 pKa = 5.12ANVDD645 pKa = 3.63NNIASVAVSIEE656 pKa = 3.58PRR658 pKa = 11.84SNDD661 pKa = 2.63EE662 pKa = 4.1CGFMFNQFSPNSDD675 pKa = 2.88GVNDD679 pKa = 4.29FLRR682 pKa = 11.84INCIEE687 pKa = 4.18TYY689 pKa = 10.3PNNTLTIFNRR699 pKa = 11.84LGSEE703 pKa = 4.48VYY705 pKa = 10.68SVTNYY710 pKa = 9.46QNNWDD715 pKa = 4.1GTWKK719 pKa = 10.74KK720 pKa = 11.07GDD722 pKa = 4.39LPNGTYY728 pKa = 10.09FYY730 pKa = 11.46VLDD733 pKa = 4.46LADD736 pKa = 3.86GSEE739 pKa = 4.29VKK741 pKa = 10.59KK742 pKa = 10.93GWIQIIRR749 pKa = 3.64

Molecular weight: 79.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0RGR8|F0RGR8_CELLC 50S ribosomal protein L32 OS=Cellulophaga lytica (strain ATCC 23178 / DSM 7489 / JCM 8516 / NBRC 14961 / NCIMB 1423 / VKM B-1433 / Cy l20) OX=867900 GN=rpmF PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 10.15RR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.29NKK16 pKa = 9.34HH17 pKa = 4.03GFRR20 pKa = 11.84EE21 pKa = 4.05RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.08GRR40 pKa = 11.84KK41 pKa = 8.3KK42 pKa = 9.93LTVSSEE48 pKa = 3.9LRR50 pKa = 11.84HH51 pKa = 5.93KK52 pKa = 10.53KK53 pKa = 10.04

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3281

0

3281

1137891

31

4782

346.8

38.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.67 ± 0.048

0.717 ± 0.016

5.582 ± 0.054

6.311 ± 0.043

5.02 ± 0.034

6.315 ± 0.051

1.609 ± 0.019

7.691 ± 0.042

8.344 ± 0.077

9.202 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.022

6.678 ± 0.044

3.286 ± 0.027

3.152 ± 0.024

3.022 ± 0.026

6.319 ± 0.034

6.421 ± 0.073

6.458 ± 0.036

1.014 ± 0.015

4.174 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski