Amino acid dipepetide frequency for Cellulophaga lytica (strain ATCC 23178 / DSM 7489 / JCM 8516 / NBRC 14961 / NCIMB 1423 / VKM B-1433 / Cy l20)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.757AlaAla: 4.757 ± 0.089
0.54AlaCys: 0.54 ± 0.026
3.673AlaAsp: 3.673 ± 0.081
4.073AlaGlu: 4.073 ± 0.077
3.186AlaPhe: 3.186 ± 0.055
3.978AlaGly: 3.978 ± 0.074
1.062AlaHis: 1.062 ± 0.035
5.613AlaIle: 5.613 ± 0.086
5.348AlaLys: 5.348 ± 0.097
6.334AlaLeu: 6.334 ± 0.096
1.462AlaMet: 1.462 ± 0.037
4.121AlaAsn: 4.121 ± 0.091
2.017AlaPro: 2.017 ± 0.057
2.361AlaGln: 2.361 ± 0.052
1.737AlaArg: 1.737 ± 0.047
4.29AlaSer: 4.29 ± 0.072
4.413AlaThr: 4.413 ± 0.112
4.42AlaVal: 4.42 ± 0.074
0.618AlaTrp: 0.618 ± 0.023
2.7AlaTyr: 2.7 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.465CysAla: 0.465 ± 0.022
0.088CysCys: 0.088 ± 0.01
0.381CysAsp: 0.381 ± 0.023
0.406CysGlu: 0.406 ± 0.018
0.416CysPhe: 0.416 ± 0.022
0.568CysGly: 0.568 ± 0.026
0.155CysHis: 0.155 ± 0.013
0.55CysIle: 0.55 ± 0.024
0.521CysLys: 0.521 ± 0.024
0.598CysLeu: 0.598 ± 0.025
0.122CysMet: 0.122 ± 0.01
0.491CysAsn: 0.491 ± 0.022
0.281CysPro: 0.281 ± 0.019
0.175CysGln: 0.175 ± 0.011
0.147CysArg: 0.147 ± 0.011
0.535CysSer: 0.535 ± 0.026
0.451CysThr: 0.451 ± 0.031
0.473CysVal: 0.473 ± 0.025
0.066CysTrp: 0.066 ± 0.008
0.279CysTyr: 0.279 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
4.188AspAla: 4.188 ± 0.142
0.39AspCys: 0.39 ± 0.02
3.047AspAsp: 3.047 ± 0.067
3.476AspGlu: 3.476 ± 0.069
3.539AspPhe: 3.539 ± 0.067
3.907AspGly: 3.907 ± 0.138
0.789AspHis: 0.789 ± 0.029
4.386AspIle: 4.386 ± 0.072
4.402AspLys: 4.402 ± 0.076
4.964AspLeu: 4.964 ± 0.064
0.994AspMet: 0.994 ± 0.032
3.594AspAsn: 3.594 ± 0.075
1.69AspPro: 1.69 ± 0.048
1.308AspGln: 1.308 ± 0.035
1.623AspArg: 1.623 ± 0.042
3.116AspSer: 3.116 ± 0.074
3.129AspThr: 3.129 ± 0.075
3.842AspVal: 3.842 ± 0.07
0.722AspTrp: 0.722 ± 0.028
2.712AspTyr: 2.712 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
4.146GluAla: 4.146 ± 0.069
0.299GluCys: 0.299 ± 0.016
3.862GluAsp: 3.862 ± 0.065
5.009GluGlu: 5.009 ± 0.102
2.805GluPhe: 2.805 ± 0.055
3.412GluGly: 3.412 ± 0.06
1.055GluHis: 1.055 ± 0.031
5.081GluIle: 5.081 ± 0.077
5.668GluLys: 5.668 ± 0.089
6.105GluLeu: 6.105 ± 0.079
1.397GluMet: 1.397 ± 0.038
4.44GluAsn: 4.44 ± 0.069
1.519GluPro: 1.519 ± 0.038
2.305GluGln: 2.305 ± 0.052
2.24GluArg: 2.24 ± 0.048
3.015GluSer: 3.015 ± 0.053
3.575GluThr: 3.575 ± 0.066
4.155GluVal: 4.155 ± 0.058
0.592GluTrp: 0.592 ± 0.025
2.431GluTyr: 2.431 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
2.955PheAla: 2.955 ± 0.056
0.446PheCys: 0.446 ± 0.02
2.81PheAsp: 2.81 ± 0.065
2.716PheGlu: 2.716 ± 0.051
2.636PhePhe: 2.636 ± 0.057
3.415PheGly: 3.415 ± 0.067
0.656PheHis: 0.656 ± 0.025
3.775PheIle: 3.775 ± 0.066
4.116PheLys: 4.116 ± 0.071
4.63PheLeu: 4.63 ± 0.079
1.097PheMet: 1.097 ± 0.033
3.637PheAsn: 3.637 ± 0.059
1.556PhePro: 1.556 ± 0.044
1.141PheGln: 1.141 ± 0.028
1.361PheArg: 1.361 ± 0.037
3.956PheSer: 3.956 ± 0.065
3.521PheThr: 3.521 ± 0.071
3.026PheVal: 3.026 ± 0.054
0.524PheTrp: 0.524 ± 0.026
2.223PheTyr: 2.223 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
4.304GlyAla: 4.304 ± 0.086
0.582GlyCys: 0.582 ± 0.04
3.543GlyAsp: 3.543 ± 0.077
3.622GlyGlu: 3.622 ± 0.062
3.474GlyPhe: 3.474 ± 0.061
4.442GlyGly: 4.442 ± 0.093
0.997GlyHis: 0.997 ± 0.031
5.117GlyIle: 5.117 ± 0.079
5.035GlyLys: 5.035 ± 0.09
5.699GlyLeu: 5.699 ± 0.076
1.392GlyMet: 1.392 ± 0.039
3.68GlyAsn: 3.68 ± 0.079
1.283GlyPro: 1.283 ± 0.035
1.68GlyGln: 1.68 ± 0.045
1.893GlyArg: 1.893 ± 0.042
3.962GlySer: 3.962 ± 0.073
4.229GlyThr: 4.229 ± 0.112
4.627GlyVal: 4.627 ± 0.07
0.711GlyTrp: 0.711 ± 0.025
2.873GlyTyr: 2.873 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
0.823HisAla: 0.823 ± 0.028
0.154HisCys: 0.154 ± 0.012
0.605HisAsp: 0.605 ± 0.022
0.66HisGlu: 0.66 ± 0.027
1.017HisPhe: 1.017 ± 0.032
0.874HisGly: 0.874 ± 0.029
0.379HisHis: 0.379 ± 0.022
1.431HisIle: 1.431 ± 0.039
1.416HisLys: 1.416 ± 0.041
1.679HisLeu: 1.679 ± 0.044
0.323HisMet: 0.323 ± 0.019
1.128HisAsn: 1.128 ± 0.034
0.754HisPro: 0.754 ± 0.025
0.649HisGln: 0.649 ± 0.026
0.57HisArg: 0.57 ± 0.024
0.96HisSer: 0.96 ± 0.032
1.021HisThr: 1.021 ± 0.033
0.729HisVal: 0.729 ± 0.026
0.196HisTrp: 0.196 ± 0.014
0.721HisTyr: 0.721 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.805IleAla: 5.805 ± 0.079
0.621IleCys: 0.621 ± 0.024
4.634IleAsp: 4.634 ± 0.069
4.878IleGlu: 4.878 ± 0.067
3.326IlePhe: 3.326 ± 0.06
5.086IleGly: 5.086 ± 0.075
1.18IleHis: 1.18 ± 0.032
5.555IleIle: 5.555 ± 0.093
6.271IleLys: 6.271 ± 0.099
6.833IleLeu: 6.833 ± 0.101
1.245IleMet: 1.245 ± 0.039
4.99IleAsn: 4.99 ± 0.087
3.017IlePro: 3.017 ± 0.049
2.157IleGln: 2.157 ± 0.045
2.129IleArg: 2.129 ± 0.041
5.33IleSer: 5.33 ± 0.074
5.565IleThr: 5.565 ± 0.114
4.83IleVal: 4.83 ± 0.079
0.661IleTrp: 0.661 ± 0.026
2.792IleTyr: 2.792 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
5.095LysAla: 5.095 ± 0.089
0.33LysCys: 0.33 ± 0.017
4.935LysAsp: 4.935 ± 0.082
6.93LysGlu: 6.93 ± 0.099
3.035LysPhe: 3.035 ± 0.053
4.734LysGly: 4.734 ± 0.075
1.467LysHis: 1.467 ± 0.039
6.426LysIle: 6.426 ± 0.097
7.911LysLys: 7.911 ± 0.117
7.096LysLeu: 7.096 ± 0.111
2.054LysMet: 2.054 ± 0.041
6.285LysAsn: 6.285 ± 0.106
2.564LysPro: 2.564 ± 0.055
2.987LysGln: 2.987 ± 0.061
2.765LysArg: 2.765 ± 0.052
4.663LysSer: 4.663 ± 0.079
5.254LysThr: 5.254 ± 0.078
4.861LysVal: 4.861 ± 0.083
0.87LysTrp: 0.87 ± 0.029
3.174LysTyr: 3.174 ± 0.069
0.0LysXaa: 0.0 ± 0.0
Leu
6.081LeuAla: 6.081 ± 0.093
0.677LeuCys: 0.677 ± 0.026
5.345LeuAsp: 5.345 ± 0.079
6.191LeuGlu: 6.191 ± 0.08
4.653LeuPhe: 4.653 ± 0.077
5.787LeuGly: 5.787 ± 0.078
1.558LeuHis: 1.558 ± 0.044
6.418LeuIle: 6.418 ± 0.097
8.104LeuLys: 8.104 ± 0.117
8.828LeuLeu: 8.828 ± 0.137
1.878LeuMet: 1.878 ± 0.042
5.745LeuAsn: 5.745 ± 0.084
3.483LeuPro: 3.483 ± 0.05
3.352LeuGln: 3.352 ± 0.062
2.836LeuArg: 2.836 ± 0.06
6.132LeuSer: 6.132 ± 0.1
5.336LeuThr: 5.336 ± 0.079
5.644LeuVal: 5.644 ± 0.081
0.772LeuTrp: 0.772 ± 0.028
3.203LeuTyr: 3.203 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
1.53MetAla: 1.53 ± 0.04
0.145MetCys: 0.145 ± 0.011
0.951MetAsp: 0.951 ± 0.033
1.142MetGlu: 1.142 ± 0.037
0.849MetPhe: 0.849 ± 0.026
1.319MetGly: 1.319 ± 0.038
0.412MetHis: 0.412 ± 0.019
1.219MetIle: 1.219 ± 0.035
1.911MetLys: 1.911 ± 0.038
1.958MetLeu: 1.958 ± 0.048
0.47MetMet: 0.47 ± 0.022
1.106MetAsn: 1.106 ± 0.033
0.801MetPro: 0.801 ± 0.027
0.89MetGln: 0.89 ± 0.027
0.768MetArg: 0.768 ± 0.028
1.386MetSer: 1.386 ± 0.044
0.983MetThr: 0.983 ± 0.031
1.381MetVal: 1.381 ± 0.036
0.17MetTrp: 0.17 ± 0.012
0.767MetTyr: 0.767 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
4.462AsnAla: 4.462 ± 0.083
0.475AsnCys: 0.475 ± 0.024
3.284AsnAsp: 3.284 ± 0.078
3.593AsnGlu: 3.593 ± 0.067
3.323AsnPhe: 3.323 ± 0.064
4.508AsnGly: 4.508 ± 0.107
0.992AsnHis: 0.992 ± 0.03
5.062AsnIle: 5.062 ± 0.071
5.325AsnLys: 5.325 ± 0.087
5.805AsnLeu: 5.805 ± 0.081
1.222AsnMet: 1.222 ± 0.034
4.956AsnAsn: 4.956 ± 0.091
2.668AsnPro: 2.668 ± 0.051
2.005AsnGln: 2.005 ± 0.044
2.007AsnArg: 2.007 ± 0.044
4.188AsnSer: 4.188 ± 0.077
4.709AsnThr: 4.709 ± 0.097
4.094AsnVal: 4.094 ± 0.071
0.84AsnTrp: 0.84 ± 0.032
3.266AsnTyr: 3.266 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
1.938ProAla: 1.938 ± 0.057
0.184ProCys: 0.184 ± 0.014
1.903ProAsp: 1.903 ± 0.052
2.577ProGlu: 2.577 ± 0.049
1.776ProPhe: 1.776 ± 0.042
1.802ProGly: 1.802 ± 0.045
0.569ProHis: 0.569 ± 0.022
2.623ProIle: 2.623 ± 0.054
2.817ProLys: 2.817 ± 0.054
2.839ProLeu: 2.839 ± 0.052
0.674ProMet: 0.674 ± 0.022
2.418ProAsn: 2.418 ± 0.056
0.721ProPro: 0.721 ± 0.032
1.0ProGln: 1.0 ± 0.035
0.794ProArg: 0.794 ± 0.029
2.124ProSer: 2.124 ± 0.04
2.146ProThr: 2.146 ± 0.057
2.257ProVal: 2.257 ± 0.047
0.335ProTrp: 0.335 ± 0.018
1.368ProTyr: 1.368 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
1.868GlnAla: 1.868 ± 0.046
0.156GlnCys: 0.156 ± 0.011
1.579GlnAsp: 1.579 ± 0.041
2.271GlnGlu: 2.271 ± 0.047
1.506GlnPhe: 1.506 ± 0.039
1.763GlnGly: 1.763 ± 0.064
0.548GlnHis: 0.548 ± 0.021
2.492GlnIle: 2.492 ± 0.046
2.877GlnLys: 2.877 ± 0.062
3.194GlnLeu: 3.194 ± 0.056
0.72GlnMet: 0.72 ± 0.024
2.123GlnAsn: 2.123 ± 0.05
0.977GlnPro: 0.977 ± 0.03
1.41GlnGln: 1.41 ± 0.041
1.075GlnArg: 1.075 ± 0.029
1.653GlnSer: 1.653 ± 0.033
1.865GlnThr: 1.865 ± 0.042
1.841GlnVal: 1.841 ± 0.04
0.345GlnTrp: 0.345 ± 0.018
1.258GlnTyr: 1.258 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
1.961ArgAla: 1.961 ± 0.049
0.168ArgCys: 0.168 ± 0.011
1.593ArgAsp: 1.593 ± 0.038
1.881ArgGlu: 1.881 ± 0.048
1.606ArgPhe: 1.606 ± 0.037
1.89ArgGly: 1.89 ± 0.045
0.47ArgHis: 0.47 ± 0.021
2.43ArgIle: 2.43 ± 0.051
2.558ArgLys: 2.558 ± 0.055
2.921ArgLeu: 2.921 ± 0.051
0.753ArgMet: 0.753 ± 0.025
1.876ArgAsn: 1.876 ± 0.041
1.0ArgPro: 1.0 ± 0.03
0.923ArgGln: 0.923 ± 0.028
1.054ArgArg: 1.054 ± 0.034
1.749ArgSer: 1.749 ± 0.045
1.672ArgThr: 1.672 ± 0.042
1.991ArgVal: 1.991 ± 0.047
0.334ArgTrp: 0.334 ± 0.017
1.39ArgTyr: 1.39 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
3.91SerAla: 3.91 ± 0.06
0.633SerCys: 0.633 ± 0.027
3.201SerAsp: 3.201 ± 0.056
3.37SerGlu: 3.37 ± 0.059
3.698SerPhe: 3.698 ± 0.065
4.228SerGly: 4.228 ± 0.083
0.884SerHis: 0.884 ± 0.027
5.118SerIle: 5.118 ± 0.074
5.301SerLys: 5.301 ± 0.084
5.958SerLeu: 5.958 ± 0.073
1.09SerMet: 1.09 ± 0.028
4.147SerAsn: 4.147 ± 0.069
1.961SerPro: 1.961 ± 0.046
1.72SerGln: 1.72 ± 0.046
1.959SerArg: 1.959 ± 0.051
4.344SerSer: 4.344 ± 0.081
3.945SerThr: 3.945 ± 0.085
4.174SerVal: 4.174 ± 0.067
0.666SerTrp: 0.666 ± 0.025
2.887SerTyr: 2.887 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
4.517ThrAla: 4.517 ± 0.119
0.352ThrCys: 0.352 ± 0.025
3.791ThrAsp: 3.791 ± 0.112
3.833ThrGlu: 3.833 ± 0.058
3.129ThrPhe: 3.129 ± 0.064
4.162ThrGly: 4.162 ± 0.092
0.982ThrHis: 0.982 ± 0.028
5.338ThrIle: 5.338 ± 0.112
4.799ThrLys: 4.799 ± 0.072
5.646ThrLeu: 5.646 ± 0.074
0.984ThrMet: 0.984 ± 0.028
4.205ThrAsn: 4.205 ± 0.103
2.678ThrPro: 2.678 ± 0.07
1.823ThrGln: 1.823 ± 0.049
1.555ThrArg: 1.555 ± 0.039
4.045ThrSer: 4.045 ± 0.086
4.561ThrThr: 4.561 ± 0.111
4.447ThrVal: 4.447 ± 0.1
0.538ThrTrp: 0.538 ± 0.026
2.822ThrTyr: 2.822 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
4.587ValAla: 4.587 ± 0.062
0.503ValCys: 0.503 ± 0.024
3.906ValAsp: 3.906 ± 0.067
3.82ValGlu: 3.82 ± 0.066
3.4ValPhe: 3.4 ± 0.058
3.982ValGly: 3.982 ± 0.066
0.967ValHis: 0.967 ± 0.029
4.752ValIle: 4.752 ± 0.07
4.661ValLys: 4.661 ± 0.078
6.246ValLeu: 6.246 ± 0.086
1.305ValMet: 1.305 ± 0.035
3.973ValAsn: 3.973 ± 0.064
2.203ValPro: 2.203 ± 0.049
1.759ValGln: 1.759 ± 0.036
1.833ValArg: 1.833 ± 0.042
4.469ValSer: 4.469 ± 0.069
4.396ValThr: 4.396 ± 0.128
4.756ValVal: 4.756 ± 0.08
0.591ValTrp: 0.591 ± 0.024
2.468ValTyr: 2.468 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.606TrpAla: 0.606 ± 0.027
0.083TrpCys: 0.083 ± 0.01
0.638TrpAsp: 0.638 ± 0.023
0.617TrpGlu: 0.617 ± 0.024
0.526TrpPhe: 0.526 ± 0.024
0.667TrpGly: 0.667 ± 0.024
0.207TrpHis: 0.207 ± 0.014
0.66TrpIle: 0.66 ± 0.024
0.772TrpLys: 0.772 ± 0.026
0.985TrpLeu: 0.985 ± 0.024
0.279TrpMet: 0.279 ± 0.016
0.638TrpAsn: 0.638 ± 0.024
0.253TrpPro: 0.253 ± 0.015
0.415TrpGln: 0.415 ± 0.022
0.386TrpArg: 0.386 ± 0.021
0.631TrpSer: 0.631 ± 0.028
0.536TrpThr: 0.536 ± 0.024
0.639TrpVal: 0.639 ± 0.027
0.152TrpTrp: 0.152 ± 0.013
0.449TrpTyr: 0.449 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.705TyrAla: 2.705 ± 0.052
0.342TyrCys: 0.342 ± 0.018
2.136TyrAsp: 2.136 ± 0.047
2.048TyrGlu: 2.048 ± 0.047
2.298TyrPhe: 2.298 ± 0.052
2.62TyrGly: 2.62 ± 0.054
0.76TyrHis: 0.76 ± 0.029
2.858TyrIle: 2.858 ± 0.056
3.619TyrLys: 3.619 ± 0.072
3.707TyrLeu: 3.707 ± 0.058
0.694TyrMet: 0.694 ± 0.028
3.228TyrAsn: 3.228 ± 0.058
1.439TyrPro: 1.439 ± 0.036
1.462TyrGln: 1.462 ± 0.037
1.49TyrArg: 1.49 ± 0.041
2.645TyrSer: 2.645 ± 0.057
2.902TyrThr: 2.902 ± 0.07
2.392TyrVal: 2.392 ± 0.052
0.438TyrTrp: 0.438 ± 0.019
1.957TyrTyr: 1.957 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3281 proteins (1137891 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski