Mesorhizobium sp. Root554

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7WF03|A0A0Q7WF03_9RHIZ 30S ribosomal protein S3 OS=Mesorhizobium sp. Root554 OX=1736557 GN=rpsC PE=3 SV=1
MM1 pKa = 7.66SSTITLVWYY10 pKa = 9.36LIVASPQGGMVVLPSTFDD28 pKa = 3.13TRR30 pKa = 11.84EE31 pKa = 3.81QCDD34 pKa = 3.68SAIVEE39 pKa = 4.43YY40 pKa = 10.2QKK42 pKa = 11.21KK43 pKa = 8.62PAPSGWQVQCVPSASPYY60 pKa = 10.36VDD62 pKa = 4.39DD63 pKa = 6.39LGAEE67 pKa = 4.27DD68 pKa = 5.19QPEE71 pKa = 3.95QPAQQ75 pKa = 3.25

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7WMB2|A0A0Q7WMB2_9RHIZ Acetyl-CoA acetyltransferase OS=Mesorhizobium sp. Root554 OX=1736557 GN=ASD44_17050 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3514

0

3514

1103888

28

2850

314.1

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.395 ± 0.057

0.769 ± 0.011

5.738 ± 0.033

5.726 ± 0.035

3.944 ± 0.027

8.692 ± 0.033

1.936 ± 0.018

5.434 ± 0.029

3.817 ± 0.038

9.843 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.018

2.694 ± 0.023

5.017 ± 0.03

2.985 ± 0.023

6.715 ± 0.038

5.487 ± 0.031

5.171 ± 0.026

7.495 ± 0.034

1.282 ± 0.017

2.263 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski