Amino acid dipepetide frequency for Mesorhizobium sp. Root554

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.09AlaAla: 17.09 ± 0.162	0.958AlaCys: 0.958 ± 0.029	7.017AlaAsp: 7.017 ± 0.089	7.705AlaGlu: 7.705 ± 0.104	4.735AlaPhe: 4.735 ± 0.074	11.265AlaGly: 11.265 ± 0.11	2.051AlaHis: 2.051 ± 0.043	6.78AlaIle: 6.78 ± 0.081	4.528AlaLys: 4.528 ± 0.082	12.71AlaLeu: 12.71 ± 0.133	3.747AlaMet: 3.747 ± 0.067	3.071AlaAsn: 3.071 ± 0.057	4.965AlaPro: 4.965 ± 0.085	3.56AlaGln: 3.56 ± 0.069	8.143AlaArg: 8.143 ± 0.091	6.682AlaSer: 6.682 ± 0.091	5.841AlaThr: 5.841 ± 0.074	9.069AlaVal: 9.069 ± 0.102	1.4AlaTrp: 1.4 ± 0.038	2.631AlaTyr: 2.631 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.905CysAla: 0.905 ± 0.03	0.091CysCys: 0.091 ± 0.01	0.481CysAsp: 0.481 ± 0.022	0.375CysGlu: 0.375 ± 0.021	0.322CysPhe: 0.322 ± 0.017	0.901CysGly: 0.901 ± 0.032	0.206CysHis: 0.206 ± 0.013	0.372CysIle: 0.372 ± 0.018	0.164CysLys: 0.164 ± 0.013	0.723CysLeu: 0.723 ± 0.025	0.152CysMet: 0.152 ± 0.01	0.194CysAsn: 0.194 ± 0.013	0.378CysPro: 0.378 ± 0.02	0.206CysGln: 0.206 ± 0.015	0.524CysArg: 0.524 ± 0.022	0.432CysSer: 0.432 ± 0.019	0.381CysThr: 0.381 ± 0.021	0.572CysVal: 0.572 ± 0.022	0.119CysTrp: 0.119 ± 0.01	0.188CysTyr: 0.188 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.812AspAla: 6.812 ± 0.088	0.445AspCys: 0.445 ± 0.021	3.085AspAsp: 3.085 ± 0.07	3.511AspGlu: 3.511 ± 0.059	2.423AspPhe: 2.423 ± 0.056	5.278AspGly: 5.278 ± 0.076	1.154AspHis: 1.154 ± 0.035	3.44AspIle: 3.44 ± 0.062	2.102AspLys: 2.102 ± 0.046	5.669AspLeu: 5.669 ± 0.083	1.547AspMet: 1.547 ± 0.035	1.471AspAsn: 1.471 ± 0.036	3.317AspPro: 3.317 ± 0.051	1.572AspGln: 1.572 ± 0.044	4.351AspArg: 4.351 ± 0.074	2.257AspSer: 2.257 ± 0.041	2.543AspThr: 2.543 ± 0.049	3.955AspVal: 3.955 ± 0.067	0.929AspTrp: 0.929 ± 0.028	1.518AspTyr: 1.518 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	7.641GluAla: 7.641 ± 0.093	0.346GluCys: 0.346 ± 0.017	2.783GluAsp: 2.783 ± 0.051	3.238GluGlu: 3.238 ± 0.056	1.904GluPhe: 1.904 ± 0.039	4.245GluGly: 4.245 ± 0.067	1.15GluHis: 1.15 ± 0.033	3.671GluIle: 3.671 ± 0.056	2.919GluLys: 2.919 ± 0.057	5.034GluLeu: 5.034 ± 0.074	1.591GluMet: 1.591 ± 0.042	1.751GluAsn: 1.751 ± 0.033	2.815GluPro: 2.815 ± 0.06	2.045GluGln: 2.045 ± 0.052	4.635GluArg: 4.635 ± 0.084	2.454GluSer: 2.454 ± 0.044	3.64GluThr: 3.64 ± 0.061	3.671GluVal: 3.671 ± 0.054	0.702GluTrp: 0.702 ± 0.027	1.025GluTyr: 1.025 ± 0.033	0.001GluXaa: 0.001 ± 0.001
Phe	4.857PheAla: 4.857 ± 0.074	0.399PheCys: 0.399 ± 0.017	2.757PheAsp: 2.757 ± 0.052	2.297PheGlu: 2.297 ± 0.049	1.613PhePhe: 1.613 ± 0.04	3.975PheGly: 3.975 ± 0.065	0.797PheHis: 0.797 ± 0.026	1.926PheIle: 1.926 ± 0.048	1.102PheLys: 1.102 ± 0.034	3.672PheLeu: 3.672 ± 0.065	0.888PheMet: 0.888 ± 0.028	1.043PheAsn: 1.043 ± 0.03	1.625PhePro: 1.625 ± 0.042	1.097PheGln: 1.097 ± 0.032	2.322PheArg: 2.322 ± 0.045	2.482PheSer: 2.482 ± 0.053	1.951PheThr: 1.951 ± 0.04	3.059PheVal: 3.059 ± 0.053	0.602PheTrp: 0.602 ± 0.025	0.972PheTyr: 0.972 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	9.147GlyAla: 9.147 ± 0.098	0.811GlyCys: 0.811 ± 0.03	4.518GlyAsp: 4.518 ± 0.06	5.007GlyGlu: 5.007 ± 0.078	3.838GlyPhe: 3.838 ± 0.057	7.556GlyGly: 7.556 ± 0.138	1.848GlyHis: 1.848 ± 0.045	5.047GlyIle: 5.047 ± 0.072	4.05GlyLys: 4.05 ± 0.062	8.841GlyLeu: 8.841 ± 0.1	2.473GlyMet: 2.473 ± 0.05	2.402GlyAsn: 2.402 ± 0.046	3.402GlyPro: 3.402 ± 0.06	2.752GlyGln: 2.752 ± 0.055	5.85GlyArg: 5.85 ± 0.074	4.848GlySer: 4.848 ± 0.068	4.596GlyThr: 4.596 ± 0.064	6.232GlyVal: 6.232 ± 0.077	1.338GlyTrp: 1.338 ± 0.033	2.364GlyTyr: 2.364 ± 0.051	0.001GlyXaa: 0.001 ± 0.001
His	2.22HisAla: 2.22 ± 0.048	0.204HisCys: 0.204 ± 0.014	1.194HisAsp: 1.194 ± 0.035	1.052HisGlu: 1.052 ± 0.029	0.827HisPhe: 0.827 ± 0.03	1.88HisGly: 1.88 ± 0.046	0.533HisHis: 0.533 ± 0.027	0.998HisIle: 0.998 ± 0.034	0.542HisLys: 0.542 ± 0.021	1.84HisLeu: 1.84 ± 0.038	0.533HisMet: 0.533 ± 0.026	0.428HisAsn: 0.428 ± 0.02	1.216HisPro: 1.216 ± 0.036	0.543HisGln: 0.543 ± 0.024	1.317HisArg: 1.317 ± 0.032	0.908HisSer: 0.908 ± 0.033	0.784HisThr: 0.784 ± 0.029	1.483HisVal: 1.483 ± 0.041	0.315HisTrp: 0.315 ± 0.019	0.544HisTyr: 0.544 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	7.552IleAla: 7.552 ± 0.092	0.523IleCys: 0.523 ± 0.022	3.898IleAsp: 3.898 ± 0.066	3.677IleGlu: 3.677 ± 0.059	1.936IlePhe: 1.936 ± 0.049	5.298IleGly: 5.298 ± 0.074	0.955IleHis: 0.955 ± 0.032	2.412IleIle: 2.412 ± 0.048	1.629IleLys: 1.629 ± 0.038	4.731IleLeu: 4.731 ± 0.073	1.112IleMet: 1.112 ± 0.034	1.432IleAsn: 1.432 ± 0.034	2.37IlePro: 2.37 ± 0.053	1.265IleGln: 1.265 ± 0.035	3.315IleArg: 3.315 ± 0.055	3.036IleSer: 3.036 ± 0.054	2.493IleThr: 2.493 ± 0.05	4.896IleVal: 4.896 ± 0.073	0.602IleTrp: 0.602 ± 0.026	1.203IleTyr: 1.203 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.991LysAla: 4.991 ± 0.071	0.159LysCys: 0.159 ± 0.013	2.106LysAsp: 2.106 ± 0.043	1.851LysGlu: 1.851 ± 0.044	1.099LysPhe: 1.099 ± 0.034	2.975LysGly: 2.975 ± 0.055	0.636LysHis: 0.636 ± 0.026	2.026LysIle: 2.026 ± 0.042	1.784LysLys: 1.784 ± 0.044	3.828LysLeu: 3.828 ± 0.064	0.939LysMet: 0.939 ± 0.029	1.082LysAsn: 1.082 ± 0.031	2.569LysPro: 2.569 ± 0.059	1.141LysGln: 1.141 ± 0.033	2.602LysArg: 2.602 ± 0.042	2.161LysSer: 2.161 ± 0.046	2.275LysThr: 2.275 ± 0.048	2.823LysVal: 2.823 ± 0.055	0.404LysTrp: 0.404 ± 0.02	0.72LysTyr: 0.72 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	13.296LeuAla: 13.296 ± 0.126	0.813LeuCys: 0.813 ± 0.026	5.901LeuAsp: 5.901 ± 0.083	5.08LeuGlu: 5.08 ± 0.079	3.796LeuPhe: 3.796 ± 0.071	8.135LeuGly: 8.135 ± 0.092	1.65LeuHis: 1.65 ± 0.042	5.047LeuIle: 5.047 ± 0.082	4.153LeuLys: 4.153 ± 0.065	9.173LeuLeu: 9.173 ± 0.124	2.326LeuMet: 2.326 ± 0.042	2.513LeuAsn: 2.513 ± 0.047	5.309LeuPro: 5.309 ± 0.062	2.618LeuGln: 2.618 ± 0.047	6.047LeuArg: 6.047 ± 0.079	6.422LeuSer: 6.422 ± 0.099	5.325LeuThr: 5.325 ± 0.067	7.548LeuVal: 7.548 ± 0.106	1.11LeuTrp: 1.11 ± 0.035	2.17LeuTyr: 2.17 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	3.376MetAla: 3.376 ± 0.054	0.134MetCys: 0.134 ± 0.013	1.241MetAsp: 1.241 ± 0.031	1.219MetGlu: 1.219 ± 0.035	0.772MetPhe: 0.772 ± 0.03	1.861MetGly: 1.861 ± 0.046	0.456MetHis: 0.456 ± 0.02	1.424MetIle: 1.424 ± 0.036	1.188MetLys: 1.188 ± 0.035	2.599MetLeu: 2.599 ± 0.042	0.713MetMet: 0.713 ± 0.03	0.919MetAsn: 0.919 ± 0.029	1.615MetPro: 1.615 ± 0.039	0.832MetGln: 0.832 ± 0.029	1.802MetArg: 1.802 ± 0.042	1.697MetSer: 1.697 ± 0.04	1.836MetThr: 1.836 ± 0.042	1.791MetVal: 1.791 ± 0.041	0.207MetTrp: 0.207 ± 0.014	0.304MetTyr: 0.304 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.313AsnAla: 3.313 ± 0.056	0.236AsnCys: 0.236 ± 0.016	1.48AsnAsp: 1.48 ± 0.04	1.313AsnGlu: 1.313 ± 0.033	1.04AsnPhe: 1.04 ± 0.032	2.545AsnGly: 2.545 ± 0.048	0.54AsnHis: 0.54 ± 0.023	1.498AsnIle: 1.498 ± 0.035	0.842AsnLys: 0.842 ± 0.032	2.496AsnLeu: 2.496 ± 0.05	0.672AsnMet: 0.672 ± 0.026	0.661AsnAsn: 0.661 ± 0.026	1.952AsnPro: 1.952 ± 0.042	0.782AsnGln: 0.782 ± 0.028	1.846AsnArg: 1.846 ± 0.041	1.316AsnSer: 1.316 ± 0.037	1.226AsnThr: 1.226 ± 0.036	2.029AsnVal: 2.029 ± 0.043	0.425AsnTrp: 0.425 ± 0.018	0.724AsnTyr: 0.724 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.244ProAla: 6.244 ± 0.101	0.265ProCys: 0.265 ± 0.014	3.518ProAsp: 3.518 ± 0.064	3.543ProGlu: 3.543 ± 0.064	2.065ProPhe: 2.065 ± 0.042	4.369ProGly: 4.369 ± 0.06	0.982ProHis: 0.982 ± 0.029	2.247ProIle: 2.247 ± 0.045	1.978ProLys: 1.978 ± 0.045	4.707ProLeu: 4.707 ± 0.069	1.222ProMet: 1.222 ± 0.039	1.371ProAsn: 1.371 ± 0.036	2.207ProPro: 2.207 ± 0.066	1.614ProGln: 1.614 ± 0.043	2.748ProArg: 2.748 ± 0.057	2.647ProSer: 2.647 ± 0.05	2.236ProThr: 2.236 ± 0.043	4.323ProVal: 4.323 ± 0.069	0.662ProTrp: 0.662 ± 0.028	1.221ProTyr: 1.221 ± 0.033	0.001ProXaa: 0.001 ± 0.001
Gln	3.894GlnAla: 3.894 ± 0.062	0.178GlnCys: 0.178 ± 0.014	1.467GlnAsp: 1.467 ± 0.038	1.512GlnGlu: 1.512 ± 0.04	1.054GlnPhe: 1.054 ± 0.032	2.29GlnGly: 2.29 ± 0.048	0.588GlnHis: 0.588 ± 0.026	1.755GlnIle: 1.755 ± 0.039	1.304GlnLys: 1.304 ± 0.036	2.632GlnLeu: 2.632 ± 0.052	0.879GlnMet: 0.879 ± 0.028	0.855GlnAsn: 0.855 ± 0.027	1.718GlnPro: 1.718 ± 0.044	1.075GlnGln: 1.075 ± 0.039	2.182GlnArg: 2.182 ± 0.043	1.713GlnSer: 1.713 ± 0.044	1.708GlnThr: 1.708 ± 0.04	2.07GlnVal: 2.07 ± 0.05	0.392GlnTrp: 0.392 ± 0.019	0.585GlnTyr: 0.585 ± 0.025	0.001GlnXaa: 0.001 ± 0.001
Arg	7.131ArgAla: 7.131 ± 0.099	0.423ArgCys: 0.423 ± 0.018	3.911ArgAsp: 3.911 ± 0.065	3.904ArgGlu: 3.904 ± 0.066	3.07ArgPhe: 3.07 ± 0.051	4.56ArgGly: 4.56 ± 0.072	1.666ArgHis: 1.666 ± 0.037	4.039ArgIle: 4.039 ± 0.055	2.633ArgLys: 2.633 ± 0.044	7.44ArgLeu: 7.44 ± 0.1	1.797ArgMet: 1.797 ± 0.039	1.907ArgAsn: 1.907 ± 0.05	3.291ArgPro: 3.291 ± 0.059	2.535ArgGln: 2.535 ± 0.051	5.256ArgArg: 5.256 ± 0.077	3.553ArgSer: 3.553 ± 0.068	3.274ArgThr: 3.274 ± 0.059	4.325ArgVal: 4.325 ± 0.062	0.846ArgTrp: 0.846 ± 0.032	1.589ArgTyr: 1.589 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.405SerAla: 6.405 ± 0.071	0.368SerCys: 0.368 ± 0.02	3.038SerAsp: 3.038 ± 0.05	2.798SerGlu: 2.798 ± 0.05	2.385SerPhe: 2.385 ± 0.051	5.811SerGly: 5.811 ± 0.077	1.103SerHis: 1.103 ± 0.031	2.965SerIle: 2.965 ± 0.055	1.766SerLys: 1.766 ± 0.045	5.476SerLeu: 5.476 ± 0.07	1.403SerMet: 1.403 ± 0.036	1.479SerAsn: 1.479 ± 0.037	2.712SerPro: 2.712 ± 0.052	1.648SerGln: 1.648 ± 0.047	3.621SerArg: 3.621 ± 0.059	2.988SerSer: 2.988 ± 0.059	2.688SerThr: 2.688 ± 0.052	4.192SerVal: 4.192 ± 0.066	0.737SerTrp: 0.737 ± 0.029	1.283SerTyr: 1.283 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	6.068ThrAla: 6.068 ± 0.082	0.361ThrCys: 0.361 ± 0.019	2.764ThrAsp: 2.764 ± 0.044	2.655ThrGlu: 2.655 ± 0.045	2.002ThrPhe: 2.002 ± 0.042	5.253ThrGly: 5.253 ± 0.07	0.958ThrHis: 0.958 ± 0.031	2.967ThrIle: 2.967 ± 0.06	1.583ThrLys: 1.583 ± 0.039	5.549ThrLeu: 5.549 ± 0.074	1.251ThrMet: 1.251 ± 0.031	1.267ThrAsn: 1.267 ± 0.035	3.035ThrPro: 3.035 ± 0.065	1.324ThrGln: 1.324 ± 0.039	3.0ThrArg: 3.0 ± 0.054	2.693ThrSer: 2.693 ± 0.045	2.702ThrThr: 2.702 ± 0.05	4.539ThrVal: 4.539 ± 0.07	0.589ThrTrp: 0.589 ± 0.025	1.144ThrTyr: 1.144 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	9.312ValAla: 9.312 ± 0.105	0.591ValCys: 0.591 ± 0.025	4.182ValAsp: 4.182 ± 0.064	4.734ValGlu: 4.734 ± 0.074	3.076ValPhe: 3.076 ± 0.055	5.792ValGly: 5.792 ± 0.079	1.314ValHis: 1.314 ± 0.037	4.08ValIle: 4.08 ± 0.065	2.623ValLys: 2.623 ± 0.05	7.494ValLeu: 7.494 ± 0.095	1.888ValMet: 1.888 ± 0.044	1.994ValAsn: 1.994 ± 0.045	3.797ValPro: 3.797 ± 0.069	1.963ValGln: 1.963 ± 0.042	4.819ValArg: 4.819 ± 0.061	4.587ValSer: 4.587 ± 0.064	4.4ValThr: 4.4 ± 0.064	5.872ValVal: 5.872 ± 0.086	0.862ValTrp: 0.862 ± 0.027	1.568ValTyr: 1.568 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.163TrpAla: 1.163 ± 0.033	0.13TrpCys: 0.13 ± 0.01	0.564TrpAsp: 0.564 ± 0.026	0.534TrpGlu: 0.534 ± 0.02	0.552TrpPhe: 0.552 ± 0.023	0.814TrpGly: 0.814 ± 0.03	0.319TrpHis: 0.319 ± 0.017	0.652TrpIle: 0.652 ± 0.023	0.52TrpLys: 0.52 ± 0.022	1.61TrpLeu: 1.61 ± 0.043	0.362TrpMet: 0.362 ± 0.018	0.476TrpAsn: 0.476 ± 0.021	0.686TrpPro: 0.686 ± 0.023	0.567TrpGln: 0.567 ± 0.027	1.064TrpArg: 1.064 ± 0.033	0.785TrpSer: 0.785 ± 0.029	0.704TrpThr: 0.704 ± 0.025	0.8TrpVal: 0.8 ± 0.029	0.238TrpTrp: 0.238 ± 0.017	0.279TrpTyr: 0.279 ± 0.015	0.002TrpXaa: 0.002 ± 0.001
Tyr	2.53TyrAla: 2.53 ± 0.05	0.249TyrCys: 0.249 ± 0.015	1.473TyrAsp: 1.473 ± 0.045	1.254TyrGlu: 1.254 ± 0.033	0.927TyrPhe: 0.927 ± 0.03	2.119TyrGly: 2.119 ± 0.047	0.454TyrHis: 0.454 ± 0.021	0.989TyrIle: 0.989 ± 0.031	0.763TyrLys: 0.763 ± 0.028	2.204TyrLeu: 2.204 ± 0.045	0.489TyrMet: 0.489 ± 0.023	0.62TyrAsn: 0.62 ± 0.026	1.191TyrPro: 1.191 ± 0.031	0.712TyrGln: 0.712 ± 0.026	1.706TyrArg: 1.706 ± 0.041	1.207TyrSer: 1.207 ± 0.034	1.105TyrThr: 1.105 ± 0.032	1.7TyrVal: 1.7 ± 0.04	0.342TyrTrp: 0.342 ± 0.016	0.598TyrTyr: 0.598 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3514 proteins (1103888 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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