Paraburkholderia fungorum

Taxonomy:

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7450 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1IWB0|A0A1H1IWB0_9BURK Threonine/homoserine/homoserine lactone efflux protein OS=Paraburkholderia fungorum OX=134537 GN=SAMN05443245_5765 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 4.46NFYY5 pKa = 10.6IAATSTSPEE14 pKa = 3.24VDD16 pKa = 3.72FRR18 pKa = 11.84FDD20 pKa = 3.74QNVLTLRR27 pKa = 11.84GEE29 pKa = 4.49SYY31 pKa = 10.68PEE33 pKa = 3.49NAAAFYY39 pKa = 11.02APVIEE44 pKa = 4.42QLRR47 pKa = 11.84TYY49 pKa = 10.83LAGCNDD55 pKa = 3.07SSITVDD61 pKa = 3.16VTLTYY66 pKa = 10.64FNSSSTKK73 pKa = 9.07MLFSVFDD80 pKa = 4.41ALDD83 pKa = 3.69EE84 pKa = 4.47AASSGNRR91 pKa = 11.84VLVNWFRR98 pKa = 11.84DD99 pKa = 3.86AEE101 pKa = 4.31DD102 pKa = 3.41EE103 pKa = 4.51TILEE107 pKa = 4.4FGEE110 pKa = 4.02EE111 pKa = 3.93LQADD115 pKa = 4.73FTAIQFTDD123 pKa = 4.02CPVATT128 pKa = 4.69

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1CHG1|A0A1H1CHG1_9BURK Hydroxymethylpyrimidine kinase /phosphomethylpyrimidine kinase OS=Paraburkholderia fungorum OX=134537 GN=SAMN05443245_2154 PE=4 SV=1
MM1 pKa = 6.8MASGRR6 pKa = 11.84RR7 pKa = 11.84VVLVTARR14 pKa = 11.84RR15 pKa = 11.84VVMTAIVVRR24 pKa = 11.84VVTLGIVPSEE34 pKa = 3.7RR35 pKa = 11.84HH36 pKa = 5.27AASKK40 pKa = 10.23VVQIVASALRR50 pKa = 11.84VALVTVRR57 pKa = 11.84RR58 pKa = 11.84VATRR62 pKa = 11.84ATVVRR67 pKa = 11.84AVTLGIAPNALRR79 pKa = 11.84AASATVRR86 pKa = 11.84RR87 pKa = 11.84VVMKK91 pKa = 9.64VIVVRR96 pKa = 11.84VVTPVIAPTGLRR108 pKa = 11.84AASVTVRR115 pKa = 11.84RR116 pKa = 11.84VAMKK120 pKa = 9.9VIVVRR125 pKa = 11.84AVTSVIAPSALRR137 pKa = 11.84AASVTVRR144 pKa = 11.84RR145 pKa = 11.84VVMTTAVRR153 pKa = 11.84AVTPVIAPNALRR165 pKa = 11.84VASATARR172 pKa = 11.84PVVMTAIVVRR182 pKa = 11.84VVTPVIAPNAHH193 pKa = 6.29PAVSKK198 pKa = 10.87AVASALRR205 pKa = 11.84VVTTANVARR214 pKa = 11.84SAARR218 pKa = 11.84ARR220 pKa = 11.84AMAVRR225 pKa = 11.84PKK227 pKa = 10.35AVRR230 pKa = 11.84AAAIAPVSATTAVPRR245 pKa = 11.84RR246 pKa = 11.84IAVAAKK252 pKa = 9.95ARR254 pKa = 11.84RR255 pKa = 11.84AASKK259 pKa = 10.84ANAVRR264 pKa = 11.84PSVANALPAASTNRR278 pKa = 11.84CRR280 pKa = 11.84PRR282 pKa = 11.84RR283 pKa = 11.84AVSATTVRR291 pKa = 11.84HH292 pKa = 5.55EE293 pKa = 4.15PTNRR297 pKa = 11.84AARR300 pKa = 11.84HH301 pKa = 5.68PLPVPNAVSLTTTTPHH317 pKa = 6.7RR318 pKa = 11.84ARR320 pKa = 11.84RR321 pKa = 11.84VAIMKK326 pKa = 7.74TRR328 pKa = 11.84RR329 pKa = 11.84ACCVCRR335 pKa = 11.84NN336 pKa = 3.55

Molecular weight: 35.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7450

0

7450

2406725

29

5743

323.1

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.415 ± 0.039

0.903 ± 0.01

5.444 ± 0.021

4.959 ± 0.03

3.765 ± 0.018

8.119 ± 0.033

2.279 ± 0.014

4.818 ± 0.021

3.147 ± 0.026

10.15 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.014

2.991 ± 0.019

5.018 ± 0.022

3.705 ± 0.02

6.609 ± 0.036

6.123 ± 0.029

5.651 ± 0.03

7.725 ± 0.025

1.367 ± 0.012

2.452 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski