Salmonella phage f18SE

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; Salmonella virus f18SE

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1Y821|A0A0K1Y821_9CAUD Uncharacterized protein OS=Salmonella phage f18SE OX=1611545 PE=4 SV=1
MM1 pKa = 7.19ATYY4 pKa = 10.6FEE6 pKa = 5.55DD7 pKa = 3.3LTKK10 pKa = 11.12AFDD13 pKa = 3.37TALAAFGTDD22 pKa = 2.97NDD24 pKa = 3.73IMVALEE30 pKa = 4.82NIDD33 pKa = 4.61APTSTDD39 pKa = 3.09TPYY42 pKa = 10.68LASYY46 pKa = 9.19MLLSDD51 pKa = 4.41TEE53 pKa = 4.16QADD56 pKa = 4.43LFWTEE61 pKa = 3.54QRR63 pKa = 11.84AGVYY67 pKa = 9.61QVDD70 pKa = 3.97INVGSALGSAPINRR84 pKa = 11.84LADD87 pKa = 3.56KK88 pKa = 11.03LNAAFAAGNCFSRR101 pKa = 11.84NEE103 pKa = 3.63ICAEE107 pKa = 4.01VQSVSLGPLIVEE119 pKa = 4.42NGWAKK124 pKa = 10.54RR125 pKa = 11.84PLSINFIAFTARR137 pKa = 11.84IRR139 pKa = 3.58

Molecular weight: 15.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1Y861|A0A0K1Y861_9CAUD Putative primase-helicase OS=Salmonella phage f18SE OX=1611545 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.7ILKK9 pKa = 10.24HH10 pKa = 6.28AGINLAPPSSAVTKK24 pKa = 10.47KK25 pKa = 7.25VTRR28 pKa = 11.84PKK30 pKa = 10.94EE31 pKa = 3.46EE32 pKa = 4.49DD33 pKa = 2.93KK34 pKa = 11.01RR35 pKa = 11.84KK36 pKa = 10.0RR37 pKa = 11.84KK38 pKa = 9.06PKK40 pKa = 10.02PRR42 pKa = 11.84VKK44 pKa = 9.94PANEE48 pKa = 3.96MPDD51 pKa = 3.59VYY53 pKa = 10.79PRR55 pKa = 11.84IPGVHH60 pKa = 4.6QPKK63 pKa = 9.16YY64 pKa = 10.53CVGKK68 pKa = 10.16GLWRR72 pKa = 11.84AHH74 pKa = 6.43SYY76 pKa = 10.54DD77 pKa = 3.01GKK79 pKa = 10.98KK80 pKa = 10.05VVNLGEE86 pKa = 4.35FSSQARR92 pKa = 11.84AHH94 pKa = 6.01MAVKK98 pKa = 10.46LYY100 pKa = 10.67KK101 pKa = 9.52LWRR104 pKa = 11.84KK105 pKa = 9.53RR106 pKa = 11.84GHH108 pKa = 6.29SDD110 pKa = 3.02IPHH113 pKa = 6.34KK114 pKa = 10.53PSIRR118 pKa = 11.84LYY120 pKa = 8.83TFRR123 pKa = 6.0

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12379

51

1032

233.6

25.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.936 ± 0.604

0.913 ± 0.12

6.212 ± 0.203

6.786 ± 0.393

3.813 ± 0.142

7.699 ± 0.352

1.591 ± 0.164

4.96 ± 0.218

6.002 ± 0.338

7.585 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.152

4.395 ± 0.227

3.942 ± 0.248

3.748 ± 0.218

5.752 ± 0.207

5.937 ± 0.26

6.285 ± 0.331

7.141 ± 0.328

1.398 ± 0.145

3.272 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski