Brevibacterium phage LuckyBarnes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Luckybarnesvirus; Brevibacterium virus LuckyBarnes

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XNW0|A0A249XNW0_9CAUD Uncharacterized protein OS=Brevibacterium phage LuckyBarnes OX=2027888 GN=SEA_LUCKYBARNES_43 PE=4 SV=1
MM1 pKa = 7.29TLNDD5 pKa = 5.26IIPAKK10 pKa = 9.57YY11 pKa = 9.77RR12 pKa = 11.84KK13 pKa = 7.71PTYY16 pKa = 10.05VVYY19 pKa = 10.84ALVGVALGAIATAYY33 pKa = 10.54GPDD36 pKa = 4.11NVPDD40 pKa = 3.46WHH42 pKa = 6.91AVTSNVYY49 pKa = 10.32LYY51 pKa = 10.41IGGAFGLTAAANTITQKK68 pKa = 10.79RR69 pKa = 11.84VGDD72 pKa = 3.75TDD74 pKa = 3.82DD75 pKa = 4.34SEE77 pKa = 5.1EE78 pKa = 4.04VDD80 pKa = 4.83PEE82 pKa = 4.31DD83 pKa = 5.72FDD85 pKa = 7.0DD86 pKa = 5.28IPLDD90 pKa = 3.34EE91 pKa = 5.8DD92 pKa = 3.62MYY94 pKa = 11.35EE95 pKa = 4.14EE96 pKa = 4.83EE97 pKa = 4.49FDD99 pKa = 3.8EE100 pKa = 4.91TPVPDD105 pKa = 5.15DD106 pKa = 3.56YY107 pKa = 11.64QPRR110 pKa = 11.84HH111 pKa = 5.21

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XNX9|A0A249XNX9_9CAUD DNA primase OS=Brevibacterium phage LuckyBarnes OX=2027888 GN=SEA_LUCKYBARNES_63 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84RR3 pKa = 11.84FGKK6 pKa = 10.53ALGLTLALAISAAGLAIASIAVGTVDD32 pKa = 4.35PIGWAVISFGLGVFGIGAALTMMWMEE58 pKa = 4.19VHH60 pKa = 7.09RR61 pKa = 11.84GG62 pKa = 3.24

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

16384

38

2154

244.5

26.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.229 ± 0.445

0.568 ± 0.115

6.641 ± 0.218

6.915 ± 0.36

2.899 ± 0.172

8.313 ± 0.481

1.776 ± 0.174

4.291 ± 0.164

4.73 ± 0.34

8.063 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.267

3.223 ± 0.154

4.779 ± 0.318

4.077 ± 0.174

6.281 ± 0.336

6.091 ± 0.3

6.891 ± 0.345

6.903 ± 0.259

1.917 ± 0.132

2.826 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski