Amino acid dipepetide frequency for Brevibacterium phage LuckyBarnes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.208AlaAla: 12.208 ± 1.471
0.244AlaCys: 0.244 ± 0.162
7.325AlaAsp: 7.325 ± 0.873
7.691AlaGlu: 7.691 ± 0.705
3.113AlaPhe: 3.113 ± 0.475
9.278AlaGly: 9.278 ± 1.287
1.465AlaHis: 1.465 ± 0.34
4.822AlaIle: 4.822 ± 0.496
4.578AlaLys: 4.578 ± 0.738
7.63AlaLeu: 7.63 ± 0.891
3.113AlaMet: 3.113 ± 0.572
2.93AlaAsn: 2.93 ± 0.677
3.784AlaPro: 3.784 ± 0.442
4.7AlaGln: 4.7 ± 0.555
6.104AlaArg: 6.104 ± 0.64
6.226AlaSer: 6.226 ± 0.75
6.775AlaThr: 6.775 ± 0.853
6.043AlaVal: 6.043 ± 0.602
1.831AlaTrp: 1.831 ± 0.367
2.442AlaTyr: 2.442 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
0.671CysAla: 0.671 ± 0.275
0.0CysCys: 0.0 ± 0.0
0.183CysAsp: 0.183 ± 0.109
0.183CysGlu: 0.183 ± 0.115
0.122CysPhe: 0.122 ± 0.077
0.671CysGly: 0.671 ± 0.217
0.061CysHis: 0.061 ± 0.065
0.244CysIle: 0.244 ± 0.141
0.183CysLys: 0.183 ± 0.099
0.305CysLeu: 0.305 ± 0.184
0.122CysMet: 0.122 ± 0.132
0.183CysAsn: 0.183 ± 0.101
0.488CysPro: 0.488 ± 0.216
0.366CysGln: 0.366 ± 0.159
0.732CysArg: 0.732 ± 0.281
0.427CysSer: 0.427 ± 0.197
0.183CysThr: 0.183 ± 0.1
0.244CysVal: 0.244 ± 0.127
0.183CysTrp: 0.183 ± 0.113
0.122CysTyr: 0.122 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
6.043AspAla: 6.043 ± 0.516
0.061AspCys: 0.061 ± 0.06
4.273AspAsp: 4.273 ± 0.59
5.31AspGlu: 5.31 ± 0.697
2.258AspPhe: 2.258 ± 0.424
4.944AspGly: 4.944 ± 0.486
1.282AspHis: 1.282 ± 0.263
2.991AspIle: 2.991 ± 0.324
3.235AspLys: 3.235 ± 0.474
6.409AspLeu: 6.409 ± 0.559
1.709AspMet: 1.709 ± 0.512
1.77AspAsn: 1.77 ± 0.514
4.029AspPro: 4.029 ± 0.533
3.052AspGln: 3.052 ± 0.437
3.601AspArg: 3.601 ± 0.415
3.113AspSer: 3.113 ± 0.334
4.578AspThr: 4.578 ± 0.451
4.334AspVal: 4.334 ± 0.615
1.221AspTrp: 1.221 ± 0.312
2.197AspTyr: 2.197 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
7.935GluAla: 7.935 ± 0.991
0.488GluCys: 0.488 ± 0.253
4.395GluAsp: 4.395 ± 0.724
5.188GluGlu: 5.188 ± 0.642
2.075GluPhe: 2.075 ± 0.384
5.066GluGly: 5.066 ± 0.447
1.16GluHis: 1.16 ± 0.29
2.808GluIle: 2.808 ± 0.408
3.418GluLys: 3.418 ± 0.642
6.409GluLeu: 6.409 ± 0.758
1.77GluMet: 1.77 ± 0.297
2.625GluAsn: 2.625 ± 0.313
1.892GluPro: 1.892 ± 0.418
2.075GluGln: 2.075 ± 0.326
3.784GluArg: 3.784 ± 0.511
5.005GluSer: 5.005 ± 0.712
4.578GluThr: 4.578 ± 0.58
4.639GluVal: 4.639 ± 0.754
1.709GluTrp: 1.709 ± 0.409
2.136GluTyr: 2.136 ± 0.358
0.0GluXaa: 0.0 ± 0.0
Phe
2.442PheAla: 2.442 ± 0.417
0.366PheCys: 0.366 ± 0.184
2.075PheAsp: 2.075 ± 0.387
1.831PheGlu: 1.831 ± 0.323
0.794PhePhe: 0.794 ± 0.282
3.113PheGly: 3.113 ± 0.501
0.671PheHis: 0.671 ± 0.207
0.855PheIle: 0.855 ± 0.218
1.404PheLys: 1.404 ± 0.25
2.319PheLeu: 2.319 ± 0.387
0.549PheMet: 0.549 ± 0.151
1.343PheAsn: 1.343 ± 0.234
1.526PhePro: 1.526 ± 0.328
0.916PheGln: 0.916 ± 0.217
1.587PheArg: 1.587 ± 0.371
2.075PheSer: 2.075 ± 0.333
1.953PheThr: 1.953 ± 0.192
1.526PheVal: 1.526 ± 0.324
0.61PheTrp: 0.61 ± 0.163
1.038PheTyr: 1.038 ± 0.257
0.0PheXaa: 0.0 ± 0.0
Gly
6.897GlyAla: 6.897 ± 0.917
0.61GlyCys: 0.61 ± 0.276
5.432GlyAsp: 5.432 ± 0.699
4.883GlyGlu: 4.883 ± 0.562
2.442GlyPhe: 2.442 ± 0.427
8.24GlyGly: 8.24 ± 1.085
1.282GlyHis: 1.282 ± 0.356
4.7GlyIle: 4.7 ± 0.665
4.639GlyLys: 4.639 ± 0.482
5.616GlyLeu: 5.616 ± 0.676
2.258GlyMet: 2.258 ± 0.35
2.503GlyAsn: 2.503 ± 0.371
3.052GlyPro: 3.052 ± 0.418
3.296GlyGln: 3.296 ± 0.406
5.371GlyArg: 5.371 ± 0.657
5.249GlySer: 5.249 ± 0.766
6.104GlyThr: 6.104 ± 0.834
6.531GlyVal: 6.531 ± 0.766
1.526GlyTrp: 1.526 ± 0.286
2.503GlyTyr: 2.503 ± 0.319
0.0GlyXaa: 0.0 ± 0.0
His
1.831HisAla: 1.831 ± 0.322
0.183HisCys: 0.183 ± 0.102
0.977HisAsp: 0.977 ± 0.298
1.038HisGlu: 1.038 ± 0.28
0.61HisPhe: 0.61 ± 0.199
1.77HisGly: 1.77 ± 0.342
0.488HisHis: 0.488 ± 0.156
0.794HisIle: 0.794 ± 0.275
0.427HisLys: 0.427 ± 0.217
1.465HisLeu: 1.465 ± 0.343
0.305HisMet: 0.305 ± 0.13
0.366HisAsn: 0.366 ± 0.122
0.977HisPro: 0.977 ± 0.296
0.794HisGln: 0.794 ± 0.244
1.526HisArg: 1.526 ± 0.285
0.855HisSer: 0.855 ± 0.216
1.282HisThr: 1.282 ± 0.366
1.343HisVal: 1.343 ± 0.309
0.305HisTrp: 0.305 ± 0.134
0.427HisTyr: 0.427 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.09IleAla: 4.09 ± 0.589
0.183IleCys: 0.183 ± 0.118
3.601IleAsp: 3.601 ± 0.512
3.113IleGlu: 3.113 ± 0.403
0.671IlePhe: 0.671 ± 0.243
2.686IleGly: 2.686 ± 0.66
0.855IleHis: 0.855 ± 0.177
2.197IleIle: 2.197 ± 0.408
2.136IleLys: 2.136 ± 0.482
3.418IleLeu: 3.418 ± 0.453
0.855IleMet: 0.855 ± 0.211
1.831IleAsn: 1.831 ± 0.374
2.136IlePro: 2.136 ± 0.4
1.526IleGln: 1.526 ± 0.293
2.075IleArg: 2.075 ± 0.335
2.747IleSer: 2.747 ± 0.405
4.334IleThr: 4.334 ± 0.503
2.808IleVal: 2.808 ± 0.441
0.488IleTrp: 0.488 ± 0.185
1.16IleTyr: 1.16 ± 0.348
0.0IleXaa: 0.0 ± 0.0
Lys
5.921LysAla: 5.921 ± 0.784
0.061LysCys: 0.061 ± 0.055
2.991LysAsp: 2.991 ± 0.458
3.113LysGlu: 3.113 ± 0.64
1.343LysPhe: 1.343 ± 0.311
3.052LysGly: 3.052 ± 0.48
0.855LysHis: 0.855 ± 0.23
2.197LysIle: 2.197 ± 0.349
2.747LysLys: 2.747 ± 0.392
4.578LysLeu: 4.578 ± 0.51
1.16LysMet: 1.16 ± 0.293
1.16LysAsn: 1.16 ± 0.248
2.381LysPro: 2.381 ± 0.46
1.465LysGln: 1.465 ± 0.364
2.869LysArg: 2.869 ± 0.434
3.235LysSer: 3.235 ± 0.666
2.808LysThr: 2.808 ± 0.487
2.686LysVal: 2.686 ± 0.407
0.671LysTrp: 0.671 ± 0.188
2.014LysTyr: 2.014 ± 0.291
0.0LysXaa: 0.0 ± 0.0
Leu
8.79LeuAla: 8.79 ± 0.821
0.549LeuCys: 0.549 ± 0.206
6.47LeuAsp: 6.47 ± 0.685
4.7LeuGlu: 4.7 ± 0.77
1.709LeuPhe: 1.709 ± 0.294
6.592LeuGly: 6.592 ± 0.811
1.648LeuHis: 1.648 ± 0.437
2.503LeuIle: 2.503 ± 0.521
3.54LeuLys: 3.54 ± 0.316
5.86LeuLeu: 5.86 ± 0.757
1.526LeuMet: 1.526 ± 0.395
2.747LeuAsn: 2.747 ± 0.405
4.334LeuPro: 4.334 ± 0.586
5.432LeuGln: 5.432 ± 0.874
6.226LeuArg: 6.226 ± 0.649
4.456LeuSer: 4.456 ± 0.474
6.165LeuThr: 6.165 ± 0.65
5.066LeuVal: 5.066 ± 0.605
1.099LeuTrp: 1.099 ± 0.216
1.221LeuTyr: 1.221 ± 0.271
0.0LeuXaa: 0.0 ± 0.0
Met
3.296MetAla: 3.296 ± 0.484
0.122MetCys: 0.122 ± 0.091
1.221MetAsp: 1.221 ± 0.339
1.709MetGlu: 1.709 ± 0.391
1.099MetPhe: 1.099 ± 0.276
1.953MetGly: 1.953 ± 0.352
0.305MetHis: 0.305 ± 0.15
1.343MetIle: 1.343 ± 0.302
1.404MetLys: 1.404 ± 0.342
1.587MetLeu: 1.587 ± 0.645
0.732MetMet: 0.732 ± 0.203
0.855MetAsn: 0.855 ± 0.221
1.465MetPro: 1.465 ± 0.296
0.61MetGln: 0.61 ± 0.199
1.709MetArg: 1.709 ± 0.361
2.258MetSer: 2.258 ± 0.284
1.953MetThr: 1.953 ± 0.327
1.343MetVal: 1.343 ± 0.306
0.366MetTrp: 0.366 ± 0.227
0.549MetTyr: 0.549 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
3.052AsnAla: 3.052 ± 0.366
0.122AsnCys: 0.122 ± 0.083
2.197AsnAsp: 2.197 ± 0.395
1.526AsnGlu: 1.526 ± 0.307
0.732AsnPhe: 0.732 ± 0.226
4.395AsnGly: 4.395 ± 0.547
0.488AsnHis: 0.488 ± 0.154
0.977AsnIle: 0.977 ± 0.232
1.038AsnLys: 1.038 ± 0.252
2.136AsnLeu: 2.136 ± 0.343
0.855AsnMet: 0.855 ± 0.242
1.099AsnAsn: 1.099 ± 0.223
2.014AsnPro: 2.014 ± 0.433
0.916AsnGln: 0.916 ± 0.295
2.319AsnArg: 2.319 ± 0.355
2.319AsnSer: 2.319 ± 0.344
2.442AsnThr: 2.442 ± 0.489
2.075AsnVal: 2.075 ± 0.253
0.794AsnTrp: 0.794 ± 0.244
0.732AsnTyr: 0.732 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
4.517ProAla: 4.517 ± 0.531
0.244ProCys: 0.244 ± 0.156
3.662ProAsp: 3.662 ± 0.648
4.09ProGlu: 4.09 ± 0.489
1.16ProPhe: 1.16 ± 0.243
4.639ProGly: 4.639 ± 0.557
0.794ProHis: 0.794 ± 0.216
1.648ProIle: 1.648 ± 0.325
2.503ProLys: 2.503 ± 0.466
3.54ProLeu: 3.54 ± 0.646
0.855ProMet: 0.855 ± 0.2
2.136ProAsn: 2.136 ± 0.333
2.258ProPro: 2.258 ± 0.489
1.831ProGln: 1.831 ± 0.437
2.381ProArg: 2.381 ± 0.433
2.442ProSer: 2.442 ± 0.436
3.174ProThr: 3.174 ± 0.473
3.723ProVal: 3.723 ± 0.606
0.855ProTrp: 0.855 ± 0.271
1.343ProTyr: 1.343 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
5.127GlnAla: 5.127 ± 0.568
0.305GlnCys: 0.305 ± 0.162
1.709GlnAsp: 1.709 ± 0.354
2.869GlnGlu: 2.869 ± 0.365
1.404GlnPhe: 1.404 ± 0.292
2.564GlnGly: 2.564 ± 0.465
1.221GlnHis: 1.221 ± 0.338
1.465GlnIle: 1.465 ± 0.265
1.465GlnLys: 1.465 ± 0.257
4.273GlnLeu: 4.273 ± 0.412
1.465GlnMet: 1.465 ± 0.307
1.465GlnAsn: 1.465 ± 0.291
1.953GlnPro: 1.953 ± 0.444
1.831GlnGln: 1.831 ± 0.441
2.991GlnArg: 2.991 ± 0.439
2.381GlnSer: 2.381 ± 0.424
1.953GlnThr: 1.953 ± 0.321
2.564GlnVal: 2.564 ± 0.506
0.794GlnTrp: 0.794 ± 0.283
0.916GlnTyr: 0.916 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
5.249ArgAla: 5.249 ± 0.802
0.488ArgCys: 0.488 ± 0.2
3.662ArgAsp: 3.662 ± 0.463
4.517ArgGlu: 4.517 ± 0.822
2.075ArgPhe: 2.075 ± 0.34
3.845ArgGly: 3.845 ± 0.469
1.16ArgHis: 1.16 ± 0.263
3.052ArgIle: 3.052 ± 0.386
2.991ArgLys: 2.991 ± 0.358
5.616ArgLeu: 5.616 ± 0.755
2.136ArgMet: 2.136 ± 0.261
1.587ArgAsn: 1.587 ± 0.311
2.258ArgPro: 2.258 ± 0.554
2.625ArgGln: 2.625 ± 0.378
4.456ArgArg: 4.456 ± 0.643
4.334ArgSer: 4.334 ± 0.57
3.723ArgThr: 3.723 ± 0.446
5.188ArgVal: 5.188 ± 0.649
1.465ArgTrp: 1.465 ± 0.374
2.381ArgTyr: 2.381 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
5.432SerAla: 5.432 ± 0.52
0.244SerCys: 0.244 ± 0.104
3.235SerAsp: 3.235 ± 0.419
4.09SerGlu: 4.09 ± 0.527
2.625SerPhe: 2.625 ± 0.399
6.043SerGly: 6.043 ± 0.661
0.916SerHis: 0.916 ± 0.232
2.564SerIle: 2.564 ± 0.376
2.442SerLys: 2.442 ± 0.445
4.639SerLeu: 4.639 ± 0.595
2.014SerMet: 2.014 ± 0.326
2.075SerAsn: 2.075 ± 0.37
2.686SerPro: 2.686 ± 0.496
2.197SerGln: 2.197 ± 0.347
3.54SerArg: 3.54 ± 0.49
3.784SerSer: 3.784 ± 0.58
3.845SerThr: 3.845 ± 0.618
5.493SerVal: 5.493 ± 0.538
1.221SerTrp: 1.221 ± 0.232
1.831SerTyr: 1.831 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
8.423ThrAla: 8.423 ± 1.053
0.488ThrCys: 0.488 ± 0.162
4.151ThrAsp: 4.151 ± 0.676
4.822ThrGlu: 4.822 ± 0.652
2.136ThrPhe: 2.136 ± 0.327
4.883ThrGly: 4.883 ± 0.543
0.855ThrHis: 0.855 ± 0.237
2.808ThrIle: 2.808 ± 0.256
3.357ThrLys: 3.357 ± 0.509
5.249ThrLeu: 5.249 ± 0.576
1.465ThrMet: 1.465 ± 0.311
2.136ThrAsn: 2.136 ± 0.447
4.334ThrPro: 4.334 ± 0.747
2.564ThrGln: 2.564 ± 0.457
3.296ThrArg: 3.296 ± 0.464
4.578ThrSer: 4.578 ± 0.636
5.066ThrThr: 5.066 ± 0.939
4.883ThrVal: 4.883 ± 0.656
1.526ThrTrp: 1.526 ± 0.256
1.892ThrTyr: 1.892 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
5.86ValAla: 5.86 ± 0.605
0.732ValCys: 0.732 ± 0.207
5.616ValAsp: 5.616 ± 0.506
5.677ValGlu: 5.677 ± 0.792
1.648ValPhe: 1.648 ± 0.418
5.31ValGly: 5.31 ± 0.679
1.099ValHis: 1.099 ± 0.252
2.808ValIle: 2.808 ± 0.395
3.723ValLys: 3.723 ± 0.386
5.371ValLeu: 5.371 ± 0.557
1.465ValMet: 1.465 ± 0.368
2.014ValAsn: 2.014 ± 0.404
4.029ValPro: 4.029 ± 0.68
2.747ValGln: 2.747 ± 0.378
4.151ValArg: 4.151 ± 0.474
3.052ValSer: 3.052 ± 0.432
5.066ValThr: 5.066 ± 0.565
5.066ValVal: 5.066 ± 0.49
1.465ValTrp: 1.465 ± 0.35
2.136ValTyr: 2.136 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
1.892TrpAla: 1.892 ± 0.477
0.061TrpCys: 0.061 ± 0.068
0.977TrpAsp: 0.977 ± 0.285
1.099TrpGlu: 1.099 ± 0.2
0.244TrpPhe: 0.244 ± 0.151
1.831TrpGly: 1.831 ± 0.267
0.61TrpHis: 0.61 ± 0.193
0.732TrpIle: 0.732 ± 0.224
1.038TrpLys: 1.038 ± 0.2
1.709TrpLeu: 1.709 ± 0.362
0.916TrpMet: 0.916 ± 0.265
0.794TrpAsn: 0.794 ± 0.218
0.977TrpPro: 0.977 ± 0.272
0.549TrpGln: 0.549 ± 0.151
1.221TrpArg: 1.221 ± 0.291
1.16TrpSer: 1.16 ± 0.402
1.16TrpThr: 1.16 ± 0.335
1.099TrpVal: 1.099 ± 0.256
0.488TrpTrp: 0.488 ± 0.188
0.61TrpTyr: 0.61 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.625TyrAla: 2.625 ± 0.371
0.122TyrCys: 0.122 ± 0.084
2.258TyrAsp: 2.258 ± 0.344
1.709TyrGlu: 1.709 ± 0.346
0.732TyrPhe: 0.732 ± 0.206
2.258TyrGly: 2.258 ± 0.363
0.549TyrHis: 0.549 ± 0.163
1.404TyrIle: 1.404 ± 0.252
1.038TyrLys: 1.038 ± 0.247
2.503TyrLeu: 2.503 ± 0.446
0.549TyrMet: 0.549 ± 0.164
0.61TyrAsn: 0.61 ± 0.18
1.221TyrPro: 1.221 ± 0.305
1.282TyrGln: 1.282 ± 0.35
2.869TyrArg: 2.869 ± 0.453
1.221TyrSer: 1.221 ± 0.251
1.77TyrThr: 1.77 ± 0.45
2.381TyrVal: 2.381 ± 0.365
0.549TyrTrp: 0.549 ± 0.246
0.61TyrTyr: 0.61 ± 0.211
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (16384 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski