Apis mellifera associated microvirus 60

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5E5|A0A3Q8U5E5_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 60 OX=2494792 PE=3 SV=1
MM1 pKa = 8.46LEE3 pKa = 3.89EE4 pKa = 3.93QGILEE9 pKa = 4.37IVLQKK14 pKa = 10.7NCNITTTPKK23 pKa = 9.6QHH25 pKa = 6.28PTGGAASATEE35 pKa = 4.18AEE37 pKa = 4.45SPEE40 pKa = 3.89YY41 pKa = 10.39LYY43 pKa = 11.42YY44 pKa = 11.2LLIIFRR50 pKa = 11.84TDD52 pKa = 2.57TFYY55 pKa = 10.56LTKK58 pKa = 10.26SEE60 pKa = 4.26KK61 pKa = 9.8VSKK64 pKa = 10.57NIQQEE69 pKa = 4.59VVV71 pKa = 2.93

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTG4|A0A3S8UTG4_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 60 OX=2494792 PE=4 SV=1
MM1 pKa = 7.01CTKK4 pKa = 10.11PLKK7 pKa = 10.39AYY9 pKa = 9.32QNPEE13 pKa = 3.25GGRR16 pKa = 11.84PIFGYY21 pKa = 9.99QGVRR25 pKa = 11.84EE26 pKa = 4.73GLPEE30 pKa = 3.85MQLPCGKK37 pKa = 10.23CSEE40 pKa = 4.48CVKK43 pKa = 10.56DD44 pKa = 5.79YY45 pKa = 9.08YY46 pKa = 9.6TAWATRR52 pKa = 11.84GFRR55 pKa = 11.84EE56 pKa = 4.06LVQWEE61 pKa = 4.04NSVFITLTYY70 pKa = 11.01DD71 pKa = 3.42NEE73 pKa = 4.26NLPGDD78 pKa = 3.81YY79 pKa = 10.37SLKK82 pKa = 10.81KK83 pKa = 10.17KK84 pKa = 10.38DD85 pKa = 3.36VQDD88 pKa = 4.41FIKK91 pKa = 10.34RR92 pKa = 11.84VKK94 pKa = 10.1KK95 pKa = 9.82HH96 pKa = 5.23FRR98 pKa = 11.84SHH100 pKa = 6.46KK101 pKa = 8.84EE102 pKa = 3.55NPVRR106 pKa = 11.84QIYY109 pKa = 9.91CGEE112 pKa = 4.09YY113 pKa = 10.05GKK115 pKa = 8.77KK116 pKa = 7.61TLRR119 pKa = 11.84PHH121 pKa = 4.81YY122 pKa = 10.12HH123 pKa = 6.15VILFNVDD130 pKa = 3.71FKK132 pKa = 11.52DD133 pKa = 3.54KK134 pKa = 10.22KK135 pKa = 9.06KK136 pKa = 10.31HH137 pKa = 4.93YY138 pKa = 9.19MSDD141 pKa = 2.75GGKK144 pKa = 9.51QVFTSEE150 pKa = 3.71ILSRR154 pKa = 11.84LWGKK158 pKa = 9.2GNCDD162 pKa = 3.14FGYY165 pKa = 10.96AEE167 pKa = 4.92PEE169 pKa = 4.04SIAYY173 pKa = 8.92LFKK176 pKa = 11.15YY177 pKa = 9.51VLKK180 pKa = 10.7KK181 pKa = 9.27KK182 pKa = 7.6TRR184 pKa = 11.84KK185 pKa = 8.95EE186 pKa = 3.87RR187 pKa = 11.84EE188 pKa = 3.56KK189 pKa = 10.72PLYY192 pKa = 9.79HH193 pKa = 6.54EE194 pKa = 5.33HH195 pKa = 7.48DD196 pKa = 3.56GCIYY200 pKa = 9.55EE201 pKa = 4.73CEE203 pKa = 4.26HH204 pKa = 6.36EE205 pKa = 4.86FIEE208 pKa = 4.92ASRR211 pKa = 11.84NPGIGACAKK220 pKa = 10.09DD221 pKa = 3.58SPSIRR226 pKa = 11.84KK227 pKa = 9.07GFLVTNGVKK236 pKa = 10.33SKK238 pKa = 10.67LPKK241 pKa = 10.4YY242 pKa = 10.25YY243 pKa = 10.56LEE245 pKa = 4.49HH246 pKa = 6.68LKK248 pKa = 9.07KK249 pKa = 9.18TDD251 pKa = 3.38LPKK254 pKa = 10.57FLEE257 pKa = 4.5IQDD260 pKa = 3.71KK261 pKa = 10.48RR262 pKa = 11.84ATFALEE268 pKa = 3.93KK269 pKa = 10.28TPEE272 pKa = 3.83TGKK275 pKa = 10.26RR276 pKa = 11.84RR277 pKa = 11.84EE278 pKa = 4.13QKK280 pKa = 10.72EE281 pKa = 4.01EE282 pKa = 3.84ALKK285 pKa = 11.15LLTDD289 pKa = 3.99TKK291 pKa = 11.19RR292 pKa = 11.84RR293 pKa = 11.84MM294 pKa = 3.39

Molecular weight: 34.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1345

71

552

224.2

25.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.361 ± 1.511

1.19 ± 0.523

5.13 ± 0.566

6.691 ± 1.542

3.569 ± 0.804

7.138 ± 0.663

1.859 ± 0.537

5.502 ± 0.706

7.435 ± 2.343

8.476 ± 0.456

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.412

4.312 ± 0.664

5.056 ± 0.394

5.651 ± 0.889

5.13 ± 0.999

6.32 ± 0.85

6.989 ± 0.977

4.833 ± 0.486

0.892 ± 0.217

3.941 ± 0.76
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski